pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO1-FLAG                7082 bp DNA     circular SYN 19-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3795
                     /codon_start=1
                     /label=ROBO1
                     /translation="VLEQPVGSSSWSSASGFIDTARRETDLLVPTRVSEKSRLLPSHQQ
                     *LTREEAKLSAVEGGLDFSTSVNLPRSHGWQTRGLILATTTMTAPSAAARQAMETATAT
                     SLPTVAQLIV*QIITTNWITNKQI*CSLSQLFMVMWTLVTKSMR*KPSIAQI*RMGVLS
                     IHQGSLLLTPPLSSSSQTSATT*TMAAGTLARSTGNHWDSRNKKWHQFSTTSWSKTS*T
                     KIIEQMTQFLQLSHTTNHTTRTQEDPTTAQTGAVVHLGVRGTRKGQEHPRYQNRVA*TG
                     QTCFLLPQHILLHTAIAKSTTFL*MKAMTKKCHVPCHQQGCICNKMN*KRRKMNEAPLP
                     LFGEQLLLQLPCPIAISPLPL*LPPHRKNSSPCYRIVQRRLATCSTSPTGDGSL*VLLH
                     HHGRSPLHIPMATFQDPWSQIWIRMRQKRKKTKPTWR*PRCKPEGFCYVGLSRHLPPVL
                     GTWRALSRGP*STAGAQPQRRTTFPADAPVLVLRTAPFSLMLTLPRQSQQRQSMLV*K*
                     HDGKCRMLLAVDIFMRLSALGPQVPCLQTAT*VPP*CRKPDQPRN*NTSQDICAEKPTQ
                     MIFHHLLCRHLL*SHLLPNPRHSWKYDL*WCQNSLLWMQEQTDHQTEKEAVTRGEKCWM
                     EDRLLTCEQIQVIPEKHRNSKMTGKDVETRQQNETFHQQRLISSKRIFYLIVDLLFQHQ
                     IIPEIPVPQAQCHQEDQEADKENKQM*VEEILQKCRYLEDMKEEKIIMKN*RKLK"
     misc_feature    1695..1700
                     /label=SDS
     misc_feature    2280..2285
                     /label=SDS
     misc_feature    2401..2406
                     /label=SDS
     misc_feature    2419..2424
                     /label=SDS
     misc_feature    2537..2542
                     /label=SDS
     misc_feature    2734..2739
                     /label=SDS
     misc_feature    2978..2983
                     /label=SDS
     misc_feature    3289..3294
                     /label=SDS
     CDS             3802..3867
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3871..4302
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4015..4020
                     /label=SDS
     repeat_region   4345..4485
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4345..4474
                     /label=AAV2 ITR
     rep_origin      4560..5015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5297..5401
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5402..6262
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6433..7021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtat tggagcagcc
     1561 tgttggatca tcctcatggt cttcagcatc tggctttatc gacaccgcaa gaagagaaac
     1621 ggacttacta gtacctacgc gggtatcaga aaagtcccgt cttttacctt cacaccaaca
     1681 gtaacttacc agagaggagg cgaagctgtc agcagtggag ggaggcctgg acttctcaac
     1741 atcagtgaac ctgccgcgca gccatggctg gcagacacgt ggcctaatac tggcaacaac
     1801 cacaatgact gctccatcag ctgctgcacg gcaggcaatg gaaacagcga cagcaacctc
     1861 actacctaca gtcgcccagc tgattgtata gcaaattata acaaccaact ggataacaaa
     1921 caaacaaatc tgatgctccc tgagtcaact gtttatggtg atgtggacct tagtaacaaa
     1981 atcaatgaga tgaaaacctt caatagccca aatctgaagg atgggcgttt tgtcaatcca
     2041 tcagggcagc ctactcctta cgccaccact cagctcatcc agtcaaacct cagcaacaac
     2101 atgaacaatg gcagcgggga ctctggcgag aagcactgga aaccactggg acagcagaaa
     2161 caagaagtgg caccagttca gtacaacatc gtggagcaaa acaagctgaa caaagattat
     2221 cgagcaaatg acacagttcc tccaactatc ccatacaacc aatcatacga ccagaacaca
     2281 ggaggatcct acaacagctc agaccggggc agtagtacat ctgggagtca ggggcacaag
     2341 aaaggggcaa gaacacccaa ggtaccaaaa cagggtggca tgaactgggc agacctgctt
     2401 cctcctcccc cagcacatcc tcctccacac agcaatagcg aagagtacaa catttctgta
     2461 gatgaaagct atgaccaaga aatgccatgt cccgtgccac cagcaaggat gtatttgcaa
     2521 caagatgaat tagaagagga ggaagatgaa cgaggcccca ctccccctgt tcggggagca
     2581 gcttcttctc cagctgccgt gtcctatagc catcagtcca ctgccactct gactccctcc
     2641 ccacaggaag aactccagcc catgttacag gattgtccag aggagactgg ccacatgcag
     2701 caccagcccg acaggagacg gcagcctgtg agtcctcctc caccaccacg gccgatctcc
     2761 cctccacata cctatggcta catttcagga cccctggtct cagatatgga tacggatgcg
     2821 ccagaagagg aagaagacga agccgacatg gaggtagcca agatgcaaac cagaaggctt
     2881 ttgttacgtg ggcttgagca gacacctgcc tccagtgttg gggacctgga gagctctgtc
     2941 acggggtcca tgatcaacgg ctggggctca gcctcagagg aggacaacat ttccagcgga
     3001 cgctccagtg ttagttcttc ggacggctcc tttttcactg atgctgactt tgcccaggca
     3061 gtcgcagcag cggcagagta tgctggtctg aaagtagcac gacggcaaat gcaggatgct
     3121 gctggccgtc gacattttca tgcgtctcag tgccctaggc ccacaagtcc cgtgtctaca
     3181 gacagcaaca tgagtgccgc cgtaatgcag aaaaccagac cagccaagaa actgaaacac
     3241 cagccaggac atctgcgcag agaaacctac acagatgatc ttccaccacc tcctgtgccg
     3301 ccacctgcta taaagtcacc tactgcccaa tccaagacac agctggaagt acgacctgta
     3361 gtggtgccaa aactcccttc tatggatgca agaacagaca gatcatcaga cagaaaagga
     3421 agcagttaca aggggagaga agtgttggat ggaagacagg ttgttgacat gcgaacaaat
     3481 ccaggtgatc ccagagaagc acaggaacag caaaatgacg ggaaaggacg tggaaacaag
     3541 gcagcaaaac gagaccttcc accagcaaag actcatctca tccaagagga tattctacct
     3601 tattgtagac ctacttttcc aacatcaaat aatcccagag atcccagttc ctcaagctca
     3661 atgtcatcaa gaggatcagg aagcagacaa agagaacaag caaatgtagg tcgaagaaat
     3721 attgcagaaa tgcaggtact tggaggatat gaaagaggag aagataataa tgaagaatta
     3781 gaggaaactg aaagctgtac agactacaaa gaccatgacg gtgattataa agatcatgac
     3841 atcgactaca aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa
     3901 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     3961 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     4021 tgtataaatc ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct
     4081 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt
     4141 gtgatgctat tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt
     4201 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4261 tttatgtttc aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt
     4321 ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     4381 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4441 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     4501 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4621 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4741 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4861 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
     4921 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4981 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     5041 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     5101 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     5161 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     5221 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     5281 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     5341 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     5401 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     5461 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     5521 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     5581 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     5641 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     5701 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     5761 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     5821 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     5881 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     5941 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     6001 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     6061 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     6121 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     6181 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     6241 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     6301 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     6361 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     6421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     6481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     6541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     6601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     6661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     6721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     6781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     6841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     6901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     7021 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     7081 gt
//