| 出品公司: | -- |
|---|---|
| 载体名称: | pBudCE4.1-hrGFP |
| 质粒类型: | 哺乳动物细胞载体 |
| 终止子: | bGH poly(A) signal |
| 复制子: | pUC |
| 启动子: | CMV |
| 载体大小: | 5303 bp |
| 5' 测序引物及序列: | CMV-F（CGCAAATGGGCGGTAGGCGTG） |
| 3' 测序引物及序列: | Bleo-R（CTGGATGATCCTCCAGCGC） |
| 载体标签: | C-His,C-V5 |
| 载体抗性: | Zeo |
| 筛选标记: | -- |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | 293T等哺乳细胞 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pBudCE4.1-hrGFP 5303 bp ds-DNA circular SYN 21-七月-2016
DEFINITION synthetic circular DNA
KEYWORDS pBudCE4.1-hrGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5303)
TITLE Direct Submission
JOURNAL Exported
http://www.biofeng.com/
FEATURES Location/Qualifiers
source 1..5303
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 10..389
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 390..593
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 638..656
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 676..1395
/codon_start=1
/product="humanized Renilla green fluorescent protein"
/note="hrGFP"
/note="mammalian codon-optimized"
/translation="MVSKQILKNTGLQEIMSFKVNLEGVVNNHVFTMEGCGKGNILFGN
QLVQIRVTKGAPLPFAFDILSPAFQYGNRTFTKYPEDISDFFIQSFPAGFVYERTLRYE
DGGLVEIRSDINLIEEMFVYRVEYKGRNFPNDGPVMKKTITGLQPSFEVVYMNDGVLVG
QVILVYRLNSGKFYSCHMRTLMKSKGVVKDFPEYHFIQHRLEKTYVEDGGFVEQHETAI
AQLTSLGKPLGSLHEWV"
CDS 1427..1456
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 1472..1489
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1520..1641
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(1771..2145)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
promoter complement(2164..2211)
/note="EM7 promoter"
/note="synthetic bacterial promoter "
promoter complement(2259..2575)
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2273..2408
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2597..3759
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 2828..3750
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
CDS 3835..3876
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 3886..3903
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 3932..4156
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin complement(4288..4876)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gcgcgcgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
61 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
121 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
181 caatagggac tttccattga cgtcaatggg tggactattt acggtaaact gcccacttgg
241 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
301 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
361 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
421 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
481 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
541 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc
601 taactagaga acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga
661 cccaagcttg ccaccatggt gagcaagcag atcctgaaga acaccggcct gcaggagatc
721 atgagcttca aggtgaacct ggagggcgtg gtgaacaacc acgtgttcac catggagggc
781 tgcggcaagg gcaacatcct gttcggcaac cagctggtgc agatccgcgt gaccaagggc
841 gcccccctgc ccttcgcctt cgacatcctg agccccgcct tccagtacgg caaccgcacc
901 ttcaccaagt accccgagga catcagcgac ttcttcatcc agagcttccc cgccggcttc
961 gtgtacgagc gcaccctgcg ctacgaggac ggcggcctgg tggagatccg cagcgacatc
1021 aacctgatcg aggagatgtt cgtgtaccgc gtggagtaca agggccgcaa cttccccaac
1081 gacggccccg tgatgaagaa gaccatcacc ggcctgcagc ccagcttcga ggtggtgtac
1141 atgaacgacg gcgtgctggt gggccaggtg atcctggtgt accgcctgaa cagcggcaag
1201 ttctacagct gccacatgcg caccctgatg aagagcaagg gcgtggtgaa ggacttcccc
1261 gagtaccact tcatccagca ccgcctggag aagacctacg tggaggacgg cggcttcgtg
1321 gagcagcacg agaccgccat cgcccagctg accagcctgg gcaagcccct gggcagcctg
1381 cacgagtggg tgtaaatcga tcttaagcag tacttctaga ggatccgaac aaaaactcat
1441 ctcagaagag gatctgaata tgcataccgg tcatcatcac catcaccatt gagtttgatc
1501 cccgggaatt cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
1561 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
1621 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
1681 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
1741 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
1801 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
1861 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
1921 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
1981 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
2041 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
2101 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
2161 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
2221 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
2281 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
2341 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
2401 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
2461 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
2521 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctcgt
2581 cgagctagct tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
2641 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
2701 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
2761 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
2821 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc
2881 ttgcgtgcct tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga
2941 gccaggggcg ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
3001 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
3061 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
3121 caagatagtc ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttgggccc
3181 gcggccggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
3241 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
3301 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
3361 gttgcgtgag cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg
3421 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
3481 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
3541 tagttctgga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
3601 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
3661 ttctcgttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
3721 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa cacgtggtcg cggccgcttc
3781 gaaggtacca gcacagtgga ctcgagagat ctggccggct gggcccgttt cgaaggtaag
3841 cctatcccta accctctcct cggtctcgat tctacgcgta ccggtcatca tcaccatcac
3901 cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
3961 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
4021 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
4081 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
4141 gcggtgggct ctatggcttc tgaggcggaa agaaccagtg gcggtaatac ggttatccac
4201 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
4261 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
4321 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
4381 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
4441 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
4501 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
4561 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
4621 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
4681 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
4741 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
4801 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
4861 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
4921 cgaaaactca cgttaaggga ttttggtcat gacattaacc tataaaaata ggcgtatcac
4981 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
5041 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
5101 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
5161 tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
5221 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
5281 cgggcctctt cgctattacg cca
//
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