pLKO.1-GFP-shRNA

价格：1500元

联系方式：I47-825O-882O

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载体基本信息

出品公司:	爱迪基因
载体名称:	pLKO.1-GFP-shRNA
质粒类型:	慢病毒载体；RNAi载体
高拷贝/低拷贝:	低拷贝
克隆方法:	gateway
启动子:	U6
载体大小:	7091 bp
5' 测序引物及序列:	LKO.1 5' ：GACTATCATATGCTTACCGT
3' 测序引物及序列:	SV40pA-R ：GAAATTTGTGATGCTATTGC
载体标签:	--
载体抗性:	氨苄青霉素、氯霉素
筛选标记:	Puromycin
克隆菌株:	Stbl3
宿主细胞（系）:	哺乳动物细胞
备注:	shRNA directed against GFP (Target sequence: 5'GCAAGCTGACCCTGAAGTTCAT3'). Used as control shRNA.
产品目录号:	30323
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	慢病毒

载体图谱质粒图谱和多克隆位点信息

载体简介

载体序列

LOCUS       pLKO.1 GFP shRNA Sequencing Result	7091 bp 	DNA	SYN
DEFINITION  pLKO.1 GFP shRNA Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..7091
                     /organism="pLKO.1 GFP shRNA Sequencing Result"
                     /mol_type="other DNA"
     promoter        446..475
                     /label="lac_promoter"
     misc_feature    489..511
                     /label="M13_pUC_rev_primer"
     promoter        510..528
                     /label="M13_reverse_primer"
     promoter        545..564
                     /label="T3_promoter"
     misc_feature    818..998
                     /label="HIV-1_5_LTR"
     misc_feature    818..998
                     /label="truncHIV-1_3_LTR"
     misc_feature    1109..1153
                     /label="HIV-1_psi_pack"
     CDS             1541..2398
                     /label="ORF frame 2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    1663..1896
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     promoter        2432..2664
                     /label="hU6_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    2594..2613
                     /label="LKO1_5_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     CDS             2707..3201
                     /label="ORF frame 1"
                     /translation="MNFRVSLLFEFSTSRQMAVFIHNFKRKGGIGGYSAGERIVDIIA
                     TDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRQPWVCAGTRLLWAWF
                     RETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVTNGS
                     RTSH*"
     misc_feature    2779..2794
                     /label="cPPT"
                     /translation="MNFRVSLLFEFSTSRQMAVFIHNFKRKGGIGGYSAGERIVDIIA
                     TDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRQPWVCAGTRLLWAWF
                     RETQRRRPWVSHILHVRSQRHPDLRRYPCGPPGDASCSAPKSGRFLAVRGVPDVTNGS
                     RTSH*"
     CDS             complement(2860..3474)
                     /label="ORF frame 3"
                     /translation="MVSSGGSPWGERSVIRSTREPTADVRSGGLQNAEHRAGRNRAHT
                     TAPHPASRTAPSRPLLSARPAEQPLLATAHRGRRAAIAPWRCPSARVLVRRAASVCHV
                     RHAANRKEPSRLRGGAGSVAGGPTRVAAKIRVTLRTDVKNVRDPGSAPLRFPEPRPEQ
                     PRPCANPGLPWKRRNPNPLEPRPKWISAVPVINPKILNFCNLFL*"
     CDS             3233..4072
                     /label="ORF frame 2"
                     /translation="MAARRPRWAVANSGCSAGRAESSGREGAVREAGCGAVVWALFLP
                     ARCSAFCKPPERTSAVGSLVDRITDLSPQGDPPELTMTEYKPTVRLATRDDVPRAVRT
                     LAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPE
                     SVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKG
                     LGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRK
                     PGA*"
     CDS             complement(3278..4123)
                     /label="ORF frame 2"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     gene            3473..4072
                     /label="puro (variant)"
                     /gene="puro (variant)"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4183..4204
                     /label="U3PPT"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4183..4198
                     /label="cPPT"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4200..4252
                     /label="delta_U3"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4253..4433
                     /label="HIV-1_5_LTR"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4253..4433
                     /label="truncHIV-1_3_LTR"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     terminator      4508..4627
                     /label="SV40_PA_terminator"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4596..4615
                     /label="EBV_rev_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     rep_origin      4645..4743
                     /label="SV40_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4728..4747
                     /label="SV40pro_F_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        complement(4822..4840)
                     /label="T7_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        complement(4850..4866)
                     /label="M13_forward20_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    4859..5007
                     /label="lacZ_a"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     misc_feature    complement(4859..4881)
                     /label="M13_pUC_fwd_primer"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     rep_origin      5025..5331
                     /label="f1_origin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     promoter        5524..5552
                     /label="AmpR_promoter"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     gene            5594..6454
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGSCAPFGRALRVVGRASGTGLAGHAPGARSFGHLDVGGDGEAE
                     PLVEGEVAGRGGLQEGGHPGALGRLHSGEHDGAAQTLALVVGRDADGGQEPRGLLGPV
                     RRQEAFHLLLRGQPGTAQLGHARADLGEHRPRFDALRRGPDRHRGAVVRDPHLADVEP
                     DAREEEFLQLGDPLDVAVRIDGVARGGVVGERGGEGAYGPGDVVAGGEAHRGLVLGHG
                     KLRWIPLGREVGDSVNEGADCRRALRRLAECGTPRGQEQGPHYRPTPRLPHRPFPAAA
                     LGAPC*"
     CDS             5594..6454
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6609..137
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
   61 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
  121 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
  181 TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG
  241 CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG
  301 AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT
  361 GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT
  421 AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG
  481 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC
  541 GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA
  601 TGCAATACTC TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG
  661 AGAGAAAAAG CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT
  721 AGGAAGGCAA CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG
  781 AGATATTGTA TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT
  841 CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT
  901 GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC
  961 CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG
 1021 AAAGCGAAAG GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC
 1081 ACGGCAAGAG GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC
 1141 TAGAAGGAGA GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA
 1201 TGGGAAAAAA TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA
 1261 TGGGCAAGCA GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA
 1321 GGCTGTAGAC AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT
 1381 AGATCATTAT ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA
 1441 GACACCAAGG AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA
 1501 CAGCAAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG
 1561 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC
 1621 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG
 1681 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC
 1741 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC
 1801 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT
 1861 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA
 1921 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA
 1981 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT
 2041 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT
 2101 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA
 2161 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT
 2221 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT
 2281 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA
 2341 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGATCAC GAGACTAGCC
 2401 TCGAGCGGCC GCCCCCTTCA CCGAGGGCCT ATTTCCCATG ATTCCTTCAT ATTTGCATAT
 2461 ACGATACAAG GCTGTTAGAG AGATAATTGG AATTAATTTG ACTGTAAACA CAAAGATATT
 2521 AGTACAAAAT ACGTGACGTA GAAAGTAATA ATTTCTTGGG TAGTTTGCAG TTTTAAAATT
 2581 ATGTTTTAAA ATGGACTATC ATATGCTTAC CGTAACTTGA AAGTATTTCG ATTTCTTGGC
 2641 TTTATATATC TTGTGGAAAG GACGAAACAC CGGGCCCGCA AGCTGACCCT GAAGTTCATT
 2701 CAAGAGATGA ACTTCAGGGT CAGCTTGCTT TTTGAATTCT CGACCTCGAG ACAAATGGCA
 2761 GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA
 2821 ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA
 2881 ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGATC CACTTTGGCC GCGGCTCGAG
 2941 GGGGTTGGGG TTGCGCCTTT TCCAAGGCAG CCCTGGGTTT GCGCAGGGAC GCGGCTGCTC
 3001 TGGGCGTGGT TCCGGGAAAC GCAGCGGCGC CGACCCTGGG TCTCGCACAT TCTTCACGTC
 3061 CGTTCGCAGC GTCACCCGGA TCTTCGCCGC TACCCTTGTG GGCCCCCCGG CGACGCTTCC
 3121 TGCTCCGCCC CTAAGTCGGG AAGGTTCCTT GCGGTTCGCG GCGTGCCGGA CGTGACAAAC
 3181 GGAAGCCGCA CGTCTCACTA GTACCCTCGC AGACGGACAG CGCCAGGGAG CAATGGCAGC
 3241 GCGCCGACCG CGATGGGCTG TGGCCAATAG CGGCTGCTCA GCAGGGCGCG CCGAGAGCAG
 3301 CGGCCGGGAA GGGGCGGTGC GGGAGGCGGG GTGTGGGGCG GTAGTGTGGG CCCTGTTCCT
 3361 GCCCGCGCGG TGTTCCGCAT TCTGCAAGCC TCCGGAGCGC ACGTCGGCAG TCGGCTCCCT
 3421 CGTTGACCGA ATCACCGACC TCTCTCCCCA GGGGGATCCA CCGGAGCTTA CCATGACCGA
 3481 GTACAAGCCC ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT
 3541 CGCCGCCGCG TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT
 3601 CGAGCGGGTC ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA
 3661 GGTGTGGGTC GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA
 3721 AGCGGGGGCG GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT
 3781 GGCCGCGCAG CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG
 3841 GTTCCTGGCC ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT
 3901 CGTGCTCCCC GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC
 3961 CGCGCCCCGC AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA
 4021 GGTGCCCGAA GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACGCCCGCC
 4081 CCACGACCCG CAGCGCCCGA CCGAAAGGAG CGCACGACCC CATGCATCGG TACCTTTAAG
 4141 ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT
 4201 GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT
 4261 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA
 4321 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC
 4381 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG
 4441 TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA
 4501 GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
 4561 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
 4621 ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT
 4681 CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG
 4741 GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC
 4801 CTAGGGACGT ACCCAATTCG CCCTATAGTG AGTCGTATTA CGCGCGCTCA CTGGCCGTCG
 4861 TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC CTTGCAGCAC
 4921 ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC CCTTCCCAAC
 4981 AGTTGCGCAG CCTGAATGGC GAATGGGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG
 5041 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT
 5101 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC
 5161 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG
 5221 ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA
 5281 CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC
 5341 CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA
 5401 AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTTAA CAAAATATTA ACGCTTACAA
 5461 TTTAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT
 5521 ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG
 5581 AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC
 5641 ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA
 5701 TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA
 5761 GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG
 5821 CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC
 5881 TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC
 5941 AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT
 6001 TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA
 6061 TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG
 6121 TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT
 6181 ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG
 6241 ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG
 6301 TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT
 6361 CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC
 6421 TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT
 6481 ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT
 6541 TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC
 6601 CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT
 6661 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC
 6721 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT
 6781 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT
 6841 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA
 6901 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC
 6961 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG
 7021 AGAAAGCGCC ACGCTTCCCg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
 7081 cggaacagga g
//

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载体质粒应用举例

上一篇：pLKO.1-puro-GFP-siRNA
下一篇：pCMV-dR8.2-dvpr

pSC101 and pKG2	pYES2-EGFP
pAX01-ComK	pME12::Tn5-259
pDEST10	pSW510
IR2	pHC79-2cos/tk
pPZP122	pcDNA3.1+/N-GST(TEV)
pIB/V5-His	pcDNA6.2/V5-PL-DEST
pRS426	pFB-hrGFP
pSilencer5.1-H1 Retro	pOS1
pCambia1201	pTALETF_v2 (NG)
pIAβ8	pFastBac-GST-C
pIJ702-87del(78-86, 88-132)	pcDNA3.1/His B
pCMV5	pKO11
pDR540	pKLAC2
pCTAP-Shuttle-A	pLenti CMV/TO Neo empty (w215-1)
pCas-Guide-EF1a-GFP	pET-28a-EGFP
pG5luc	pGL4.77[hRlucP/Hygro]
pMSCG19	pcDNA4/V5-His B
pLVX-Myc-MCS-Strep-IRES-Puro	pZeoSV2 (+)
pTYB2	pGL4.25[luc2CP/minP]
DR2	pCB1535
pCAMBIA3200	pTT5
pCR4-35	pFtsH_TA
pAc5.1a	pBT-5
pB-gyrase_TA	pVW5JI
pEF1/His B	pBApo-CMV-Pur
pDP5rs	pE
pHIC-PI	pMKO.1-GFP
pECFP-Nuc	pFN31K Nluc-CMV-neo-Flexi
pWW60-1	pMIB/V5-His A
pET302/NT-His	pGFP22
pJW342	pTCK303
PX391	pCas
pFA1	pYES2-kan
p53-Luc	pEF4/His B
pGL4.83[hRlucP/Puro]	pUCDM
pLVX-rHom-Sec1	VaD1
pXIS11	pET-12c(+)
pTrcHis2 C	pLKO-DEST-EGFP
phCMV1	pMMB33
p3xFLAG-KV2.1	pGL101
pET-32 EK/LIC	pCold-GST
pPH3	pQE-32
pCMV-3Tag-3a	pAc5.1-V5-His A
pMV261	pcDNA3.1+P2A-eGFP
pSRE-Luc	pCDF1-MCS2-EF1-Puro
pSinRep5	pcDNA4/HisMax A
pMYs-IRES-GFP	pBHR68
pNIT-3	pFastBac1
pacAd5 9.2-100	pGL4.39[luc2P/ATF6 RE/Hygro]
pFastBac Dual	pBad/Myc-His A

cytoplasmic EKAR (Cerulean-Venus)	pHIV-iRFP720-E2A-Luc
pRN11	pmGFP
FUGW-PercevalHR	pminiTol2
pCMVR8.74	mTert-pBabe-puro
pPH37	pHIV-Luc-ZsGreen
LentiGuide Cherry	pF151 pcDNA3.1(+)SOD1WT
pRK5-p18-HA	pC0061 PsmCas13 (B05) His6-TwinStrep-SUM
pAAV-EF1a-DIO-HTB	pSLQ9926: pHR-PGK-SV40_NLS-dCasMINI-V4-V
pCMV-HsPeg10rc3	Tcf/Lef:H2B-GFP
pLV-puro-N-3HA-SREBP1-C-Flag	mTagBFP2-TOMM20-N-10
Stat3 Flag pRc/CMV	pHyo094 (eMutaT7)
pHAGE2-TetOminiCMV-mCherry	pRJPaph-bjGFP
BECLIN-HA WT	pEGFP-NMHC II-C
pCMV-ABE7.9	pc DNA FRT TO- APPSwed/Ind
pET21a-BirA	xCas9(3.6)-BE3
pBabe-hygro PyMT	pAAV-EF1a-DIO-mCherry
pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T	MSP680
EK0285 SFFV-mCherry-SG(s70587)SG-EGFP (F	pGL4-ERSE2-luc2P-Hygro
pNLF1W	pSIN4-EF1a-LMO2-IRES-Puro
pUCmini-iCAP-AAV.CAP-B22	pSBbi-GN
CaV1.2	pCMVHA hEZH2
pBS-hBAF60a	pCMV-SynA
pGL3-mArg1 promoter/enhancer -31/-3810	pEcgRNA
pTK299	TrkC-GFP
pBAD18-Cm	SIRT3 Flag
Flag-TRIM24	pCas9
BCR/ABL P190 transgenic construct	pCMV-2NLS-Cas9-6XMS2
pMXs-hc-MYC	pCAS9-TPC
pmScarlet_Giantin_C1	pBabe-puro-miR-10b sponge
pcDNA3.1 myc-NL-GW	pDA077
pY001 (pFnCpf1_full)	pNI3
pGL3 2 kb prom. CD274	pMSCV-FlagMLL-pl-ENL(5613)
pGM1202	pJQ200mp18
pcDNA3.1-mCherry	Plasmid L5 attB::Pleft* mCherry
pHAtC	MSP2135
pMDIAI	pCI neo-hEST2
pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2	pTNT-B18R-6His
pYLCRISPR/Cas9pUbi-N	pBFC1207
hCas9_D10A	pLentipuro3/TO/V5-GW/EGFP-Firefly Lucife
DNMT3b KO Hyg	pHAGE NFkB-TA-LUC-UBC-GFP-W
pCRISPR-aBEST	pR26 GFP Dest
pRK GFP Paxillin	pSELECT-HA-mFOXO1
pPBbsr2-4031NES	pEJS654 All-in-One AAV-sgRNA-hNmeCas9
PG13-luc (wt p53 binding sites)	pLenti PGK Puro DEST (w529-2)
p415-GalL-Cas9-CYC1t	pMaster1
pFE-sfGFP	pBECKP-km
pCMVInt	pET15b_Bst-LF
pCAG Cas9-2A-Citrine	pcDNA3/Flag-METTL3
pGIPZ-PD-L1-EGFP	sfGFP-Cx43K258stop
pHAGE2-TetOminiCMV-SKM	jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcode

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pLKO.1-GFP-shRNA

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