pLZ368

LOCUS       pLZ368                3276 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..3276
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    19..153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    23..214
                     /label=SV40 late polyA
     rep_origin      complement(498..1126)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(516..1104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1573..1951)
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     promoter        complement(2136..2240)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(2177..2205)
                     /label=Amp prom
     misc_feature    2272..2321
                     /label=2XSTAT3
     misc_feature    2334..2383
                     /label=2XSTAT3
     misc_feature    2390..2439
                     /label=2XSTAT3
     misc_feature    2446..2495
                     /label=2XSTAT3
     misc_feature    2510..2658
                     /label=P-hCMVmin
     promoter        2532..2570
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2643..2658)
                     /label=oYW11
     primer_bind     2650..2695
                     /label=oLH179 Nluc F
     misc_feature    2672..2740
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     primer_bind     2672..2696
                     /label=olyl291
     primer_bind     2672..2695
                     /label=olyl052
     primer_bind     2672..2695
                     /label=olyl054
     primer_bind     2672..2691
                     /label=oYS11
     primer_bind     2759..2783
                     /label=oLH200 Nluc F
     misc_feature    2762..3271
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     misc_feature    2792..2794
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(join(3254..3276,1..6))
                     /label=oLH180 Nluc R
     primer_bind     complement(3254..3274)
                     /label=oLH201 Nluc R
     primer_bind     complement(3254..3271)
                     /label=oYS12
ORIGIN
        1 ggccgcaaag ctagcttcga tccagacatg ataagataca ttgatgagtt tggacaaacc
       61 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
      121 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
      181 tttcaggttc agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
      241 ggtatggctg attatgatcc ggctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
      301 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
      361 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
      421 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
      481 gagagtgcac catatcgggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
      541 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
      601 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
      661 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
      721 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
      781 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
      841 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
      901 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
      961 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     1021 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     1081 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     1141 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     1201 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     1261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1861 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     1921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgtcgacatt
     2281 tcccgtaaat cgtcgagtcg acatttcccg taaatcgtcg agggcccatc gatgtcgaca
     2341 tttcccgtaa atcgtcgagt cgacatttcc cgtaaatcgt cgagggcccg tcgacatttc
     2401 ccgtaaatcg tcgagtcgac atttcccgta aatcgtcgaa tcgatgtcga catttcccgt
     2461 aaatcgtcga gtcgacattt cccgtaaatc gtcgaacgcg tcctgcaggt cgagctcggt
     2521 acccgggtcg aggtaggcgt gtacggtggg aggcctatat aagcagagct cgtttagtga
     2581 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
     2641 accgatccag cctccgcgga attcgccacc tatgactagt gagacagaca cactcctgct
     2701 atgggtactg ctgctctggg ttccaggttc cactggtgac gctagtggtg gttctggtat
     2761 ggtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct acaacctgga
     2821 ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg tgtccgtaac
     2881 tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca tccatgtcat
     2941 catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa tttttaaggt
     3001 ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca cactggtaat
     3061 cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag gcatcgccgt
     3121 gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca aaattatcga
     3181 cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca acggagtgac
     3241 cggctggcgg ctgtgcgaac gcattctggc gtaagc
//