| 质粒名称: | pSBbi G418R mbIL2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 194329 |
| 存储实验室: | Alexander McLellan |
|
载体骨架基本信息 |
|
| 载体名称: | pSBbi |
| 载体出品公司: | -- |
| 空载体大小: | 5835 bp |
| 完整质粒大小: | 7040 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达 |
| 筛选标记: | 新霉素（G418筛选） |
| 载体抗性: | 氨苄青霉素，100μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | mbIL-21 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1206 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | EF1-a |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | TCAAGCCTCAGACAGTGGTTC |
| 3’测序引物 | TAGAAGGCACAGTCGAGG |
|
文献引用方法 |
|
| 材料和方法部分: | pSBbi G418R mbIL21 was a gift from Alexander McLellan (Addgene plasmid # 194329 ; http://n2t.net/addgene:194329 ; RRID:Addgene_194329) |
| 参考文献部分: | -- |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pSBbi G418R mbIL2 7040 bp ds-DNA circular SYN 30-JAN-2023 DEFINITION Consitiutive mammalian expression of membrane bound human IL-21. ACCESSION . VERSION . KEYWORDS pSBbi G418R mbIL21 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7040) TITLE McLellan lab plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 7040) AUTHORS . TITLE Direct Submission JOURNAL Exported Jan 30, 2023 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..7040 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(1268..1285) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1274..1498 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1930..2034 /gene="bla" /label=AmpR promoter CDS 2035..2895 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(2253..2272) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 3066..3654 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3555..3574 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3808..3825 /label=L4440 /note="L4440 vector, forward primer" primer_bind 4199..4218 /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" polyA_signal 4217..4272 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind 4318..4337 /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" CDS complement(4412..5206) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4523..4542) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 5133..5152 /label=Neo-R /note="Neomycin resistance gene, reverse primer" promoter 5839..7020 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" primer_bind 6968..6988 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" ORIGIN 1 ctgaggccac catgtggctt caatctctgt tgctcttggg cacggttgcg tgcagtatca 61 gtcaagggca agatagacac atgattcgga tgcggcagtt gattgatatt gtggaccaac 121 tcaaaaacta tgtcaatgat ctcgtccctg agtttctccc tgcgccggaa gacgttgaaa 181 ctaattgtga gtggagtgcc ttttcttgct ttcaaaaggc tcagcttaag agtgccaata 241 ctgggaacaa tgagcgaatc atcaatgtca gcatcaaaaa actgaagcga aagcctcctt 301 caacgaatgc cggtcgaagg caaaagcacc ggttgacgtg tcccagttgt gatagctacg 361 aaaagaagcc ccctaaagaa tttctcgaaa gattcaaaag tctgttgcaa aagatgatcc 421 accagcatct cagttctcgg acgcatggca gtgaagactc cgagtccaaa tatgggcctc 481 cgtgtccacc atgtcccgct ccagaatttt tgggaggacc tagcgtattt ctgtttcctc 541 cgaaaccaaa ggatacgttg atgatttctc ggactccgga ggtgacctgt gtcgtagtcg 601 atgtatcaca agaggacccc gaggtgcagt tcaattggta cgtcgatggc gtcgaagtac 661 acaatgccaa gaccaagcct agagaggaac agtttaactc tacgtacagg gtagtatcag 721 ttttgacagt gctgcatcag gattggctca acggcaagga gtacaaatgt aaagtttcca 781 ataagggtct cccctcatcc attgagaaga ctatctcaaa ggctaaaggg cagcccagag 841 aaccacaggt atatacactg ccccctagtc aggaagagat gacgaaaaat caagtatcct 901 tgacgtgctt ggttaaagga ttctatccta gcgatattgc tgtagaatgg gagtctaatg 961 ggcagcccga gaataattat aagaccactc ccccagtgtt ggattcagac gggtccttct 1021 ttctttactc ccgccttacc gttgataaga gtcggtggca ggaagggaat gtattttcat 1081 gttccgtgat gcacgaggca cttcataacc attacactca gaagtctttg agtttgagct 1141 tgggcaagat ggcgttgatc gtacttggcg gtgtcgcggg tctgttgctt ttcatcggac 1201 tgggaatatt tttctaaggc ctgtcaggcc aagcttgggg atcaattctc tagagctcgc 1261 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg 1321 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt 1381 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc 1441 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct 1501 tctgaggcgg aaagaaccag ctgggcctac tagatagcgg accccttacc gaaacatcgc 1561 cgcattctgc agaggagtcg aacttagtgt atgtaaactt ctgacccact ggaattgtga 1621 tacagtgaat tataagtgaa ataatctgtc tgtaaacaat tgttggaaaa atgacttgtg 1681 tcatgcacaa agtagatgtc ctaactgact tgccaaaact attgtttgtt aacaagaaat 1741 ttgtggagta gttgaaaaac gagttttaat gactccaact taagtgtatg taaacttccg 1801 acttcaactg tatagggatc caattttaac aaaatattaa cgcttacaat ttcctgatgc 1861 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catcaggtgg cacttttcgg 1921 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 1981 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 2041 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 2101 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 2161 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 2221 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 2281 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 2341 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 2401 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 2461 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 2521 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 2581 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 2641 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 2701 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 2761 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 2821 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 2881 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 2941 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 3001 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 3061 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 3121 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 3181 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 3241 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 3301 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 3361 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 3421 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 3481 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 3541 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 3601 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 3661 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 3721 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 3781 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggatccctat 3841 acagttgaag tcggaagttt acatacacct tagccaaata catttaaact cactttttca 3901 caattcctga catttaatcc tagtaaaaat tccctgtctt aggtcagtta ggatcaccac 3961 tttattttaa gaatgtgaaa tatcagaata atagtagaga gaatgattca tttcagcttt 4021 tatttctttc atcacattcc cagtgggtca gaagtttaca tacactcgac tcctctgcag 4081 aatgcggcga tgtttcggta aggggtcccg agtagctagt tcatggcagc cagcatatgg 4141 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc 4201 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa 4261 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt 4321 tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagcca 4381 ccaccttctg ataggcagcc tgcacctgag gtcagaagaa ctcgtcaaga aggcgataga 4441 aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc 4501 attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt 4561 ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga 4621 tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg 4681 ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat 4741 cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt 4801 ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca 4861 tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt 4921 cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag 4981 gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg 5041 caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca 5101 cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca 5161 cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatggtg gcctcaggtg 5221 cagaggtttc tacagggaaa gagagaaagg ctacagttag tttgctgtga tgatgctaag 5281 tgccgaggtc agatgtctat ggattgtaca cggaatccca acgcaggaac caaaaaacac 5341 tcacccaaaa aaatggcgct aaggccgaga gaaaggcgaa gttccgtcta cggctatata 5401 ccttgaaaca ggggcggcag aagtgacgca acgcggttgc gcggacggct tcctgtgttt 5461 tcattggtcg aagcgttctg cgcgtgcgca gagattcccc agggaaggcg ggccctcttc 5521 cgaatgtcca atccggacat tcttccggtt ggacctccgc ggtcagcgct gtgcccggcg 5581 agagatcacg tggggcgcgg aggcggtgct gctggggcac ggccgtccag cctcggcggc 5641 catatttttg aggggctgtt catctcgttc acacgctctg tccgccatgt ttgtgagtgg 5701 aagcgccatt accccttcaa gcgactgaag gctgcagggc ctctggtggc ccgcatgggg 5761 agaccagacc cgccaggccc gcctttccgc actcagtccg ggcttacttt attttgtgag 5821 acagggtctc gcctagaggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag 5881 tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga aggtggcgcg 5941 gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag 6001 aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca 6061 gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg ggttatggcc 6121 cttgcgtgcc ttgaattact tccacgcccc tggctgcagt acgtgattct tgatcccgag 6181 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct 6241 cgtgcttgag ttgaggcctg gcttgggcgc tggggccgcc gcgtgcgaat ctggtggcac 6301 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct 6361 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact 6421 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt 6481 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct 6541 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca 6601 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct 6661 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca 6721 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg 6781 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg 6841 ggggaggggt tttatgcgat ggagtttccc catactgagt gggtggagac tgaagttagg 6901 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg 6961 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttaa ggtgtcgtga 7021 aaactacccc aagctggcct //
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