| 质粒名称: | pLVPT-rtTR-KRAB-2SM2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11652 |
| 存储实验室: | Patrick Aebischer, Didier Trono |
|
载体骨架基本信息 |
|
| 载体名称: | -- |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 11645 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mPGK, GFP, rtTR-KRAB-2SM2, Tet-off |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLVPT-rtTR-KRAB-2SM2 was a gift from Patrick Aebischer & Didier Trono (Addgene plasmid # 11652 ; http://n2t.net/addgene:11652 ; RRID:Addgene_11652) |
| 参考文献部分: | A versatile tool for conditional gene expression and knockdown. Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P. Nat Methods. 2006 Feb . 3(2):109-16. 10.1038/nmeth846 PubMed 16432520 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVPT-rtTR-KRAB-2SM2 11645 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Tet-regulated (Tet-off) lentiviral vector for transgene (hPGK
promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM
(Addgene#12247)) - 2nd generation.
ACCESSION .
VERSION .
KEYWORDS pLVPT-rtTR-KRAB-2SM2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11645)
AUTHORS Szulc J, Wiznerowicz M, Sauvain MO, Trono D, Aebischer P
TITLE A versatile tool for conditional gene expression and knockdown.
JOURNAL Nat Methods. 2006 Feb . 3(2):109-16.
PUBMED 16432520
REFERENCE 2 (bases 1 to 11645)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11645
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 12..645
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 692..817
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1310..1543
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2038..2155
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2194..2693
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2229..2252)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2626..2645
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
regulatory 2744..2753
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2750..3466
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2795..2816)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(3056..3075)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3403..3424
/label=EGFP-C
/note="EGFP, forward primer"
misc_feature 3562..4113
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3706..3723)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3933..3952
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 4737..4757
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 4860..5054
/codon_start=1
/gene="ZNF10"
/gene_synonym="KOX1"
/product="Kruppel-associated box (KRAB) transcriptional
repression domain from the human zinc finger protein ZNF10
(Margolin et al., 1994)"
/label=KRAB
/translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
QLTKPDVILRLEKGEEPWLV"
primer_bind complement(5192..5208)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5193..5209)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_feature 5235..5823
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(5288..5308)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(5706..5717)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
protein_bind 5961..6231
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
LTR 6270..6450
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
promoter complement(6475..6493)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(6476..6493)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(6783..6802)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(7016..7035)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7135..7157
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7195..7213)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7281..7385
/gene="bla"
/label=AmpR promoter
CDS 7386..8246
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7604..7623)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8417..9005
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8906..8925
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9159..9176
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(9246..9266)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9251..9580
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9431..9566
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9493..9512
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 10714..10779
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 10909..10929
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 11354..11488
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11391..11410)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11445..11464
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
61 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
121 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
181 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
241 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
301 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
361 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
421 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
481 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
541 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
601 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
661 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
721 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
781 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
841 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
901 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
961 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
1021 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
1081 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
1141 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
1201 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
1261 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
1321 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg acgctgacgg
1381 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
1441 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
1501 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
1561 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
1621 tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
1681 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
1741 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
1801 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
1861 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
1921 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
1981 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc ggttaacttt
2041 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
2101 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
2161 tcacgagact agcctcgacg atggtcgagt accgggtagg ggaggcgctt ttcccaaggc
2221 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
2281 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
2341 ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
2401 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
2461 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
2521 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
2581 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
2641 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctcta
2701 gcgggatcca ggcctaagct tacgcgtcct agcgctaccg gtcgccacca tggtgagcaa
2761 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
2821 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
2881 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
2941 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
3001 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
3061 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
3121 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
3181 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
3241 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
3301 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
3361 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
3421 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtccg gactcagatc
3481 tcgactagct agtagctagc tagctagtcg agctcaagct tcgaattccc gggctgcagg
3541 aattccgccc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
3601 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
3661 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
3721 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
3781 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
3841 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
3901 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
3961 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
4021 catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga accacgggga
4081 cgtggttttc ctttgaaaaa cacgatgata ataccatggc tagactggac aagagcaaag
4141 tcataaacgg cgctctggaa ttactcaatg gagtcggtat cgaaggcctg acgacaagga
4201 aactcgctca aaagctggga gttgagcagc ctaccctgta ctggcacgtg aagaacaagc
4261 gggccctgct cgatgccctg ccaatcgaga tgctggacag gcatcatacc cacttctgcc
4321 ccctggaagg cgagtcatgg caagactttc tgcggaacaa cgccaagtca ttccgctgtg
4381 ctctcctctc acatcgcgac ggggctaaag tgcatctcgg cacccgccca acagagaaac
4441 agtacgaaac cctggaaaat cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga
4501 acgcactgta cgctctgtcc gccgtgggcc actttacact gggctgcgta ttggaggaac
4561 aggagcatca agtagcaaaa gaggaaagag agacacctac caccgattct atgcccccac
4621 ttctgagaca agcaattgag ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg
4681 gcctggaact aatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtcgccaa
4741 aaaagaagag aaaggtcgac ggcggtggtg ctttgtctcc tcagcactct gctgtcactc
4801 aaggaagtat catcaagaac aaggagggca tggatgctaa gtcactaact gcctggtccc
4861 ggacactggt gaccttcaag gatgtatttg tggacttcac cagggaggag tggaagctgc
4921 tggacactgc tcagcagatc gtgtacagaa atgtgatgct ggagaactat aagaacctgg
4981 tttccttggg ttatcagctt actaagccag atgtgatcct ccggttggag aagggagaag
5041 agccctggct ggtggagaga gaaattcacc aagagaccca tcctgattca gagactgcat
5101 ttgaaatcaa atcatcagtt taaggatctc gactagctag tagctagcta gctagtcgag
5161 ctcaacttcg aattcgatat caagcttatc gcgataccgt cgacctcgac ctcgacctcg
5221 actagtcata tgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
5281 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
5341 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
5401 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
5461 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
5521 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
5581 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
5641 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
5701 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
5761 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
5821 cgcatcggta cgtatggcca ggtaccttta agaccaatga cttacaaggc agctgtagat
5881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
5941 caagatctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
6001 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
6061 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
6121 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
6181 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
6241 gtcggatcga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
6301 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
6361 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
6421 ccttttagtc agtgtggaaa atctctagca gcatctagaa ttaattccgt gtattctata
6481 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
6541 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
6601 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
6661 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
6721 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
6781 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
6841 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
6901 ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
6961 ctgcttccta atgcaggagt cgcataaggg agagcgtcga atggtgcact ctcagtacaa
7021 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
7081 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
7141 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
7201 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
7261 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
7321 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
7381 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
7441 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
7501 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
7561 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
7621 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
7681 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
7741 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
7801 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
7861 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
7921 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
7981 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
8041 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
8101 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
8161 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
8221 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
8281 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
8341 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
8401 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
8461 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
8521 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
8581 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
8641 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
8701 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
8761 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg
8821 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
8881 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
8941 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
9001 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
9061 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
9121 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
9181 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
9241 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
9301 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
9361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
9421 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
9481 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
9541 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
9601 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
9661 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
9721 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
9781 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
9841 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
9901 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
9961 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
10021 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
10081 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
10141 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
10201 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
10261 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
10321 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
10381 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
10441 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
10501 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
10561 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
10621 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
10681 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
10741 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
10801 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
10861 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
10921 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
10981 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
11041 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
11101 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
11161 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
11221 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
11281 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
11341 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
11401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
11461 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
11521 caaacttccc accccatacc ctattaccac tgccaattac ctagtggttt catttactct
11581 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
11641 ctaca
//
