pCMV-SPORT6.1

价格:2500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Invitrogen
载体名称: pCMV-SPORT6.1
质粒类型: 哺乳动物表达载体;cDNA克隆载体;I.M.A.G.E.载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点;Gateway载体
启动子: CMV
载体大小: 4177 bp
5' 测序引物及序列: CMV fwd 5’CGCAAATGGGCGGTAGGCGTG 3’
3' 测序引物及序列: SV40pA rev 5'GAAATTTGTGATGCTATTGC'
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: --
克隆菌株: DH5α
宿主细胞(系): 哺乳动物细胞
备注:
pCMV-SPORT6.1有多个限制性酶切位点,又可用于Gateway克隆,用于cDNA文库的构建和表达。
 
产品目录号: --
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pCMV-SPORT6.1载体图谱
pCMV-SPORT6.1多克隆位点

载体简介

载体序列

LOCUS       Exported                4177 bp ds-DNA     circular SYN 12-AUG-2016
DEFINITION  Gateway(R) library vector for cloning and transient mammalian cell 
            expression of cDNAs. See pCMV SPORT6 for an older version of this 
            vector.
KEYWORDS    pCMV SPORT6.1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Invitrogen (Life Technologies)
COMMENT     The cDNA is inserted between bases 781-782, destroying an EcoRV site
            present in the original vector.
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     polyA_signal    125..259
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     primer_bind     664..680
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        691..709
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    711..735
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB2"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    736..806
                     /note="MCS"
     protein_bind    complement(807..831)
                     /gene="mutant version of attB"
                     /bound_moiety="BP Clonase(TM)"
                     /note="attB1"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(832..850)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(861..877)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1003..1206)
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        1207..1510
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(1970..2558)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2729..3589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3590..3694)
                     /gene="bla"
                     /note="AmpR promoter"
     rep_origin      complement(3721..4176)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      121 gatcgatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
      181 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
      241 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
      301 ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta tggctgatta
      361 tgatcatgaa cagactgtga ggactgaggg gcctgaaatg agccttggga ctgtgaatct
      421 aaaatacaca aacaattaga atcactagct cctgtgtata atattttcat aaatcatact
      481 cagtaagcaa aactctcaag cagcaagcat atgcagctag tttaacacat tatacactta
      541 aaaattttat atttacctta gagctttaaa tctctgtagg tagtttgtcc aattatgtca
      601 caccacagaa gtaaggttcc ttcacaaaga tcccaagcta gcagttttcc cagtcacgac
      661 gttgtaaaac gacggccagt gcctagctta taatacgact cactataggg accactttgt
      721 acaagaaagc tgggtacgcg taagcttggg cccctcgagg gatactctag agcggccgcc
      781 catcccggga attccggacc ggtaccagcc tgcttttttg tacaaacttg ttctatagtg
      841 tcacctaaat aggcctaatg gtcatagctg tttcctgtgt gaaattgtta tccgctccgc
      901 ggcctaggct agagtccgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca
      961 gcgtggatgg cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac
     1021 ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt
     1081 tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag
     1141 acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg
     1201 catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca
     1261 taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg
     1321 gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt accgtaaata
     1381 ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat
     1441 tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag
     1501 ttatgtaacg acatgcatct aatgagtgaa agggcctcgt actacgccta tttttatagg
     1561 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     1621 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1681 aataaccctg ataaatgctt caataatatt gaaaaacgcg cgaattgcaa gctctgcatt
     1741 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     1801 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     1861 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     1921 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1981 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2041 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2101 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2161 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2221 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2281 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2341 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2401 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2461 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2521 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2581 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     2641 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     2701 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     2761 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     2821 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     2881 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     2941 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     3001 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     3061 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     3121 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     3181 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     3241 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     3301 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     3361 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     3421 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     3481 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     3541 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     3601 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     3661 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     3721 aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     3781 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     3841 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     3901 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     3961 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4021 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4081 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4141 cacccgccgc gcttaatgcg ccgctacagg gcgcgtc
//

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