pAAV-cFos3-mAvicFP1-iPAK4

LOCUS       pAAV-cFos3-mAvicFP1-iPAK4              6210 bp DNA     circular SYN 23-APR-2024
DEFINITION  Express FlicR1 preferentially in neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul 
            A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE
  TITLE     A Bright and Fast Red Fluorescent Protein Voltage Indicator That 
            Reports Neuronal Activity in Organotypic Brain Slices.
  JOURNAL   J Neurosci. 2016 Feb 24;36(8):2458-72. doi: 
            10.1523/JNEUROSCI.3484-15.2016.
  PUBMED    26911693
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3484-15"; journalName: "J Neurosci"; date: 
            "2016-02-24- 24"; volume: "36"; issue: "8"; pages: "2458-72"
FEATURES             Location/Qualifiers
     source          1..6210
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          577..894
                     /lab_host="."
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1631..2707
                     /mol_type="genomic DNA"
                     /organism="."
     protein_bind    229..250
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        265..295
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    303..319
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     308..330
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     327..343
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     327..343
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   381..510
                     /label=AAV2 ITR
     primer_bind     555..605
                     /label=PstI-cfos-shortF
     promoter        577..894
                     /label=putative_fos
                     /note="/created_by=xiuyuanli
                     https://www.ncbi.nlm.nih.gov/nucleotide/V00727.1?report=gen
                     bank&log$=nuclalign&blast_rank=3&RID=55DC32WT016: 1-1618"
     promoter        577..894
                     /label=putative_fos_from34856
                     /note="/created_by=xiuyuanli"
                     /note="/modified_by=xiuyuanli"
     primer_bind     complement(872..916)
                     /label=MCS-shortCfos-R
     CDS             914..1630
                     /codon_start=1
                     /product="https://www.addgene.org/browse/article/28203833/"
                     /label=mAvicFP1
                     /translation="MVSKGEELFNGIVPILIELNGDVHGHKFSVRGEGEGDAGSGKIEI
                     KFVCTTGTLPVPWPTLVTTLCYGVQCFTRYPEHMKQHDFYKSAMPDGYIQKRTISFQDD
                     GHYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGNKMEYNYNSHSVYVLSDKANNGIK
                     VNFKIRHNLKGEGIQLADHDQQNIPIGDGPVLLPDYHYLSTQTKITKDPNEKRDHMNLV
                     EFVTACGITHGMDELYK"
     regulatory      914..917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1610..1656
                     /label=GFP-iPAK4-F
     primer_bind     1610..1656
                     /label=Primer 1a
     CDS             1643..1756
                     /codon_start=1
                     /label=INKA-box
                     /translation="EAEDWTAALLNRGRSRQPLVLGDNCFADLVHNWMELPE"
     CDS             1763..2704
                     /codon_start=1
                     /label=Pak4cat
                     /translation="PAAPAVPGPPGPRSPQREPQRVSHEQFRAALQLVVDPGDPRSYLD
                     NFIKIGEGSTGIVCIATVRSSGKLVAVKKMDLRKQQRRELLFNEVVIMRDYQHENVVEM
                     YNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIAAVCLAVLQALSVLHAQGVIHRD
                     IKSDSILLTHDGRVKLSDFGFCAQVSKEVPRRKSLVGTPYWMAPELISRLPYGPEVDIW
                     SLGIMVIEMVDGEPPYFNEPPLKAMKMIRDNLPPRLKNLHKVSPSLKGFLDRLLVRDPA
                     QRATAAELLKHPFLAKAGPPASIVPLMRQNRTR"
     primer_bind     complement(1856..1874)
                     /label=PAK4-R
     primer_bind     complement(2687..2729)
                     /label=HindIII-PAK4-R
     misc_feature    2720..3308
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2773..2793)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3191..3202)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3311..3327)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3312..3328)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3357..3478
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3368..3387)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3422..3441
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3460..3478)
                     /label=SV40-polyA
     primer_bind     complement(3581..3595)
                     /label=PstI-cfos-shortF
     repeat_region   3640..3769
                     /label=AAV2 ITR
     primer_bind     complement(3788..3805)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3788..3804)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3797..3819)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3946..4401
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4033..4052)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4243..4264
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4427..4531
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4532..5392
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4750..4769)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5563..6151
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6052..6071
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
       61 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
      121 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
      181 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
      241 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
      301 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccagatt
      361 taattaaggc cttaattagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc
      421 ccgggcgtcg ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg
      481 gagtggccaa ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact
      541 tatctacgta gccatgctct aggaagatct ctgcagggat gtccatatta ggacatctgc
      601 gtcagcaggt ttccacggcc ggtccctgtt gttctggggg ggggaccatc tccgaaatcc
      661 tacacgcgga aggtctagga gaccccctaa gatcccaaat gtgaacactc ataggtgaaa
      721 gatgtatgcc aagacggggg ttgaaagcct ggggcgtaga gttgacgaca gagcgcccgc
      781 agagggcctt ggggcgcgct tcccccccct tccagttccg cccagtgacg taggaagtcc
      841 atccattcac agcgcttcta taaaggcgcc agctgaggcg cctactactc caactcgaat
      901 tcggggatcc accatggtga gcaagggcga ggagctgttc aacggtatcg ttcctatcct
      961 gatcgaactg aacggtgacg ttcatggtca taagttctct gttcgtggtg aaggtgaagg
     1021 tgacgctggt tctggtaaga tcgaaatcaa gttcgtttgc accaccggta ccctgcctgt
     1081 tccttggcct accctggtta ccaccttatg ctacggtgtt cagtgcttca cccgttaccc
     1141 tgaacatatg aagcagcatg acttctacaa gtctgctatg cctgacggtt acatccagaa
     1201 gcgtaccatc tctttccagg acgacggtca ttacaagacc cgtgctgaag ttaagttcga
     1261 aggtgacacc ctggttaacc gtatcgaact gaagggtatc gacttcaagg aagacggtaa
     1321 catcctgggt aacaagatgg aatacaacta caactctcat tctgtttacg ttctgtctga
     1381 caaggctaac aacggtatca aggttaactt caagatccgt cataacctga agggtgaagg
     1441 tatccagctg gctgaccatg accagcagaa catccctatc ggtgacggtc ctgttctgct
     1501 gcctgactac cattacctgt ctacccagac caagatcacc aaggacccta acgaaaagcg
     1561 tgaccatatg aacctggttg aattcgttac cgcttgcggt atcacccatg gcatggacga
     1621 gctgtacaag tcaggtggat ccgaagcaga ggactggaca gcagccctac tgaacagggg
     1681 tcgcagtcgc cagcccctgg tactagggga caattgcttt gctgacttgg tgcacaactg
     1741 gatggagctg cctgaggaat tccccgccgc ccctgctgtt cctgggcccc ctggcccccg
     1801 ctcaccacag cgggagccac agcgagtatc ccatgagcag ttccgggctg ccctgcagct
     1861 ggtggtggac ccaggcgacc cccgctccta cctggacaac ttcatcaaga ttggcgaggg
     1921 ctccacgggc atcgtgtgca tcgccaccgt gcgcagctcg ggcaagctgg tggccgtcaa
     1981 gaagatggac ctgcgcaagc agcagaggcg cgagctgctc ttcaacgagg tggtaatcat
     2041 gagggactac cagcacgaga atgtggtgga gatgtacaac agctacctgg tgggggacga
     2101 gctctgggtg gtcatggagt tcctggaagg aggcgccctc accgacatcg tcacccacac
     2161 caggatgaac gaggagcaga tcgcagccgt gtgccttgca gtgctgcagg ccctgtcggt
     2221 gctccacgcc cagggcgtca tccaccggga catcaagagc gactcgatcc tgctgaccca
     2281 tgatggcagg gtgaagctgt cagactttgg gttctgcgcc caggtgagca aggaagtgcc
     2341 ccgaaggaag tcgctggtcg gcacgcccta ctggatggcc ccagagctca tctcccgcct
     2401 tccctacggg ccagaggtag acatctggtc gctggggata atggtgattg agatggtgga
     2461 cggagagccc ccctacttca acgagccacc cctcaaagcc atgaagatga ttcgggacaa
     2521 cctgccaccc cgactgaaga acctgcacaa ggtgtcgcca tccctgaagg gcttcctgga
     2581 ccgcctgctg gtgcgagacc ctgcccagcg ggccacggca gccgagctgc tgaagcaccc
     2641 attcctggcc aaggcagggc cgcctgccag catcgtgccc ctcatgcgcc agaaccgcac
     2701 cagatgaaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2761 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2821 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2881 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2941 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3001 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3061 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3121 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3181 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3241 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     3301 ctccccgcat cgataccgtc gacctcgacc cgggcggccg cttcgagcag acatgataag
     3361 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3421 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     3481 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     3541 aagcaagtaa aacctctaca aatgtggtaa aatcgataag gatcttccta gagcatggct
     3601 acgtagataa gtagcatggc gggttaatca ttaactacaa ggaaccccta gtgatggagt
     3661 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc
     3721 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc cttaattaac
     3781 ctaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3841 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3901 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3961 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     4021 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     4081 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     4141 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     4201 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     4261 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     4321 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4381 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4441 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4501 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4561 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4621 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4681 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4741 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4801 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4861 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4921 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4981 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     5041 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     5101 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     5161 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     5221 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     5281 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     5341 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5401 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5461 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5521 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5581 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5641 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5701 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5761 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5821 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5881 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5941 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6001 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6061 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6121 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6181 cctggccttt tgctggcctt ttgctcacat
//