pGL4.50[luc2/CMV/Hygro]

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pGL4.50载体基本信息

出品公司: Promega
载体名称: pGL4.50 ; pGL4.50[luc2/CMV/Hygro]
质粒类型: 信号通路报告载体;哺乳动物载体;荧光素酶报告载体
高拷贝/低拷贝: --
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 6600 bp
5' 测序引物及序列: CMV-F : TCTAAAAGCTGCGGAATTGT
3' 测序引物及序列: --
载体标签: luc2
载体抗性: 氨苄青霉素
筛选标记: 潮霉素(Hygromycin)
克隆菌株: TOP10等常规菌株
宿主细胞(系): HEK293等
备注: pGL4.50[luc2P]载体是信号通路报告载体;含荧光素酶报告基因luc2。
产品目录号: E1310
稳定性: 瞬表达或稳表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒

pGL4.50载体质粒图谱和多克隆位点信息

pGL4.50载体图谱



pGL4.50 多克隆位点

pGL4.50 载体特征

pGL4.50载体简介

pGL4.50载体简介
The pGL4.50[luc2/CMV/Hygro] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis), which has been codon optimized for mammalian expression. This vector also is engineered with fewer consensus regulatory sequences for reduced backgrounds and a decreased risk of anomalous transcription. This vector contains the following features: luc2 reporter gene for expression in mammalian cells; CMV promoter for high translational expression; SV40 late poly(A) signal sequence is positioned downstream of luc2 to provide efficient transcription termination and mRNA polyadenylation; Binding region for RV primer 3 and RV primer 4; Synthetic poly(A) signal/transcription start site; Synthetic Hygromycin B-resistance gene for mammalian cell selection of the plasmid; Plasmid replication origin; Ampr gene for bacterial selection for vector amplification.

pGL4.50载体序列

LOCUS       EU921840                6600 bp    DNA     linear   SYN 28-AUG-2008
DEFINITION  Luciferase reporter vector pGL4.50[luc2/CMV/Hygro], complete
            sequence.
ACCESSION   EU921840
VERSION     EU921840.1  GI:197215832
KEYWORDS    .
SOURCE      Luciferase reporter vector pGL4.50[luc2/CMV/Hygro]
  ORGANISM  Luciferase reporter vector pGL4.50[luc2/CMV/Hygro]
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Brouette,C., Hung,L. and Kopish,K.
  TITLE     pGL4 Luciferase Reporter Vectors Technical Manual, TM259
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Brouette,C., Hung,L. and Kopish,K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-JUL-2008) SciComm, Promega, 5500 E Cheryl Parkway,
            Madison, WI 53711, USA
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Luciferase reporter vector
                     pGL4.50[luc2/CMV/Hygro]"
                     /mol_type="other DNA"
                     /db_xref="taxon:554672"
     regulatory      14..755
                     /regulatory_class="promoter"
                     /note="CMV immediate early enhancer/promoter"
     gene            859..2511
                     /gene="luc2"
     CDS             859..2511
                     /gene="luc2"
                     /note="luminescent reporter protein"
                     /codon_start=1
                     /transl_table=11
                     /product="firefly luciferase"
                     /protein_id="ACH53163.1"
                     /db_xref="GI:197215833"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
                     AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
                     APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
                     FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
                     FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
                     LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
                     GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
                     RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
                     PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
                     AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    2546..2767
                     /note="SV40 late poly(A) region"
     regulatory      2815..3233
                     /regulatory_class="promoter"
                     /note="SV40 early enhancer/promoter"
     gene            3258..4295
                     /gene="HygroR"
     CDS             3258..4295
                     /gene="HygroR"
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin resistant protein"
                     /protein_id="ACH53164.1"
                     /db_xref="GI:197215834"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKEVGRV"
     misc_feature    4319..4367
                     /note="poly(A) region"
     primer_bind     complement(4434..4453)
                     /note="RV primer 4 binding region"
     gene            complement(5482..6342)
                     /gene="AmpR"
     CDS             complement(5482..6342)
                     /gene="AmpR"
                     /note="confers resistance to ampicillin"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ACH53165.1"
                     /db_xref="GI:197215835"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     misc_feature    6447..6600
                     /note="poly(A) signal/transcription pause region"
     primer_bind     6549..6568
                     /note="RV primer 3 binding region"
ORIGIN      
        1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
       61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
      121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
      181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
      241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
      301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
      361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
      421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
      481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
      541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
      601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
      661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
      721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
      781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
      841 actgttggta aagccaccat ggaagatgcc aaaaacatta agaagggccc agcgccattc
      901 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc
      961 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc
     1021 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca
     1081 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt
     1141 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg
     1201 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa
     1261 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc
     1321 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc
     1381 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg
     1441 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc
     1501 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac
     1561 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc
     1621 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg
     1681 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc
     1741 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc
     1801 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca
     1861 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa
     1921 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg
     1981 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc
     2041 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag
     2101 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc
     2161 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg
     2221 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac
     2281 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc
     2341 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt
     2401 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag
     2461 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataattctag
     2521 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     2581 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     2641 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     2701 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     2761 tgtggtaaaa tcgataagga tccgtttgcg tattgggcgc tcttccgctg atctgcgcag
     2821 caccatggcc tgaaataacc tctgaaagag gaacttggtt agctaccttc tgaggcggaa
     2881 agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     2941 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     3001 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     3061 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     3121 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     3181 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcgattctt
     3241 ctgacactag cgccaccatg aagaagcccg aactcaccgc taccagcgtt gaaaaatttc
     3301 tcatcgagaa gttcgacagt gtgagcgacc tgatgcagtt gtcggagggc gaagagagcc
     3361 gagccttcag cttcgatgtc ggcggacgcg gctatgtact gcgggtgaat agctgcgctg
     3421 atggcttcta caaagaccgc tacgtgtacc gccacttcgc cagcgctgca ctacccatcc
     3481 ccgaagtgtt ggacatcggc gagttcagcg agagcctgac atactgcatc agtagacgcg
     3541 cccaaggcgt tactctccaa gacctccccg aaacagagct gcctgctgtg ttacagcctg
     3601 tcgccgaagc tatggatgct attgccgccg ccgacctcag tcaaaccagc ggcttcggcc
     3661 cattcgggcc ccaaggcatc ggccagtaca caacctggcg ggatttcatt tgcgccattg
     3721 ctgatcccca tgtctaccac tggcagaccg tgatggacga caccgtgtcc gccagcgtag
     3781 ctcaagccct ggacgaactg atgctgtggg ccgaagactg tcccgaggtg cgccacctcg
     3841 tccatgccga cttcggcagc aacaacgtcc tgaccgacaa cggccgcatc accgccgtaa
     3901 tcgactggtc cgaagctatg ttcggggaca gtcagtacga ggtggccaac atcttcttct
     3961 ggcggccctg gctggcttgc atggagcagc agactcgcta cttcgagcgc cggcatcccg
     4021 agctggccgg cagccctcgt ctgcgagcct acatgctgcg catcggcctg gatcagctct
     4081 accagagcct cgtggacggc aacttcgacg atgctgcctg ggctcaaggc cgctgcgatg
     4141 ccatcgtccg cagcggggcc ggcaccgtcg gtcgcacaca aatcgctcgc cggagcgcag
     4201 ccgtatggac cgacggctgc gtcgaggtgc tggccgacag cggcaaccgc cggcccagta
     4261 cacgaccgcg cgctaaggag gtaggtcgag tttaaactct agaaccggtc atggccgcaa
     4321 taaaatatct ttattttcat tacatctgtg tgttggtttt ttgtgtgttc gaactagatg
     4381 ctgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     4441 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     4501 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     4561 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     4621 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     4681 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     4741 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     4801 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     4861 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     4921 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     4981 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     5041 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     5101 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     5161 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     5221 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     5281 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     5341 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     5401 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca
     5461 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat
     5521 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg
     5581 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc
     5641 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc
     5701 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc
     5761 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc
     5821 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc
     5881 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa
     5941 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat
     6001 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta
     6061 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca
     6121 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag
     6181 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc
     6241 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc
     6301 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata
     6361 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa
     6421 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg
     6481 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     6541 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//

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