| 质粒名称: | pAAV-AscI-CAG-NES-NeIle |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000341 |
| 产品用途: | Cytoplasmic expression of green Nelle Isoleucin sensor in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-CAG |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | Nelle |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
NeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein
Asanova A.N., Subach O.M., Myachina S.A., Evteeva M.A., Gunitseva N.M., Borisova A.A., Patrushev M.V., Vlaskina A.V., Nikolaeva A.Y., Yang L., Gabdulkhakov A., Dronova E., Samygina V.R., Xiao X., Zhao H., Piatkevich K.D., Subach F.V.
ACS Sensors 9, 10, 5135–5147 (2024)
|
pAAV-AscI-CAG-NES-NeIle
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-AscI-CAG-NES-NeIle载体质粒基本信息
pAAV-AscI-CAG-NES-NeIle质粒图谱载体图谱和pAAV-AscI-CAG-NES-NeIle载体序列质粒序列多克隆位点信息
pAAV-AscI-CAG-NES-NeIle质粒载体简介
pAAV-AscI-CAG-NES-NeIle质粒序列载体序列
LOCUS pAAV-AscI-CAG-NES-NeIle 6601 bp DNA circular SYN 15-OCT-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-AscI-CAG-NES-NeIle
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6601)
AUTHORS Vax
TITLE Direct Submission
JOURNAL Exported Jul 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6601
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin complement(182..770)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 2661..2691
/label=AscI-OxE insert
/label=AscI-OxE\insert
/note="/vntifkey=21"
misc_feature 2692..2935
/label=CMV enhancer
/label=CMV\enhancer
/note="/vntifkey=21"
misc_feature 2942..3219
/label=chicken b-actin promoter
/label=chicken\b-actin\promoter
/note="/vntifkey=21"
CDS 3643..3678
/codon_start=1
/label=NES
/note="/vntifkey=21"
/translation="LQLPPLERLTLS"
CDS 3685..5472
/codon_start=1
/label=NeIle
/note="/vntifkey=21"
/translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
QILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKHIVDAVK
AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
GFEFGVFDWHANGTATDAK"
misc_feature 5503..6091
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 6134..6255
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature join(6601,1..940)
/label=ColE1
/note="ColE1"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
3601 gctgtctcat cattttggca aagaattgga tccgacacca tgcttcaact tcctcctctt
3661 gaacgtctta ctctttcgag atctatggaa gatattaaag tcgcggtcgt gggcgcaatg
3721 tccgctggtg ctgactggcg tatatccaag gaggcttgcc ccaatggtaa gactcttatt
3781 tccacactta agtggtctta taccaccggc gacggcaaac gctaccggtc tactgctcgc
3841 accacattca cattcgccaa accaatggcc gcaaattacc tcaaaaatca gccgatgtat
3901 gtcttccgga gaacagagct gaaacattct aagaccgaaa tggattttaa agagtggcaa
3961 aaggccttta cggacgtggt gggcatggac gagctgtaca aggtggatgg cggcagcggt
4021 ggcaccggca tggtctcaga gggagaggtg gacaacatgg ctagtctgcc tgctacccac
4081 gaactgcata tttttggctc cataaatggc gtcggtttcg acatggtagg ccaaggctcc
4141 ggtaatccaa acgtcggtta tgaggagctg aacctgaagt ccaccaaggg cgaactgcag
4201 ttcagtccct ggatcctggt gccgcacatt gggtatgggt ttcatcagta tcttccctac
4261 ccggacggga tgtcgccatt tcaggctgca atggtcgacg ggtccggata tcaggtgcac
4321 cgcactgtgc ggtttgagga cggtgcttca ctgaccgtga attacagata cacctacgaa
4381 ggatcacata tcaaagggga agctcaggtg aagggcactg ggttcccggc agacggccct
4441 gtgatgacaa attccctgac agcctttcat attggtccgg tgacccagta cggtgaccag
4501 gagtttaccg gcgcagagca ggcggttgcg gatatcaacg ctagaggcgg cattaagggc
4561 aacaaactgc aaatcgtaaa atatgacgat gcctgtgacc cgaaacaggc tgttgcggtg
4621 gcgaacagag tcgttaacga cggcattaaa tatgtgattg gtcacctctg ttcttcatca
4681 gcgcagcctg cgtctgacat ctacgaagac gcaggcattt taatgatcac cccagcggca
4741 accgcgccgg agctgaccgc ccgtggctat cagctgatcc tgcgcaccac cggcctggac
4801 tccgaccagg ggctgacggc ggcgaaatat attcttgaga aagtgaaacc gcagcgtatt
4861 gctatcgttc acgacaaaca gcagtacggc gaaggtctgg cgcgagcggt gcagggtggc
4921 ctgaagaaag gcaatgcaaa cgtggtgttc tttgatggca tcaccgccgg ggaaaaagat
4981 ttctcaacgc tggtggcgcg tctgaaaaaa gagaatatcg acttcgttta ctacggcggt
5041 tatcacccgg aaatggggca aatcctgcgt caggcacgcg cggcagggct gaaaactcag
5101 tttatggggc cggagggtgt ggctaacgtt tcgctgtcta acattgcggg cgaatcagcg
5161 gaagggctgc tggtgaccaa gccgaagaac tacgatcagg ttccggcgaa caaacacatt
5221 gttgacgcag tcaaagcgaa aaaacaggac ccaagtggcg cattcgtttg gaccacctac
5281 gccgcgctgc aatctttgca ggcgggcctg aatcagtctg acgatccggc tgaaatcgcc
5341 aaatacctga aagcgaactc cgtggatacc gtaatgggac cgctgacctg ggatgagaaa
5401 ggcgatctga aaggctttga gttcggcgta tttgactggc acgccaacgg cacggcgacc
5461 gatgcgaagt aaactagtga attcgatatc aagcttatcg ataatcaacc tctggattac
5521 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
5581 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
5641 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
5701 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
5761 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
5821 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
5881 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
5941 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
6001 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
6061 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacccgg gcggccgctt
6121 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
6181 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
6241 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
6301 atgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
6361 cttcctagag catggctacg tagataagta gcatggcggg ttaatcatta actacaagga
6421 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
6481 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
6541 gcgcagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
6601 t
//
