| 质粒名称: | pAAV-AscI-CAG-dMito2-NeIle |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000342 |
| 产品用途: | Mitochondrial expression of green Nelle Isoleucin sensor in mammalian cells |
| 存储实验室: | Laboratory of Molecular Design |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-CAG |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | COX8A, Nelle |
| 物种来源: | Human, Synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
NeIle, a Genetically Encoded Indicator for Branched-Chain Amino Acids Based on mNeonGreen Fluorescent Protein and LIVBP Protein
Asanova A.N., Subach O.M., Myachina S.A., Evteeva M.A., Gunitseva N.M., Borisova A.A., Patrushev M.V., Vlaskina A.V., Nikolaeva A.Y., Yang L., Gabdulkhakov A., Dronova E., Samygina V.R., Xiao X., Zhao H., Piatkevich K.D., Subach F.V.
ACS Sensors 9, 10, 5135–5147 (2024)
|
pAAV-AscI-CAG-dMito2-NeIle
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-AscI-CAG-dMito2-NeIle载体质粒基本信息
pAAV-AscI-CAG-dMito2-NeIle质粒图谱载体图谱和pAAV-AscI-CAG-dMito2-NeIle载体序列质粒序列多克隆位点信息
pAAV-AscI-CAG-dMito2-NeIle质粒载体简介
pAAV-AscI-CAG-dMito2-NeIle质粒序列载体序列
LOCUS pAAV-AscI-CAG-dMito2-NeIle 6763 bp DNA circular SYN 15-OCT-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAAV-AscI-CAG-dMito2-NeIle
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6763)
AUTHORS Vax
TITLE Direct Submission
JOURNAL Exported Jul 7, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6763
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(941..1801)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(1802..1906)
/gene="bla"
/label=AmpR promoter
rep_origin complement(1932..2387)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
misc_feature 2661..2691
/label=AscI-OxE insert
/label=AscI-OxE\insert
/note="/vntifkey=21"
misc_feature 2692..2935
/label=CMV enhancer
/label=CMV\enhancer
/note="/vntifkey=21"
promoter 2942..3219
/label=chicken beta-actin promoter
CDS 3646..3720
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="MSVLTPLLLRGLTGSARRLPVPRAK"
CDS 3748..3819
/codon_start=1
/product="mitochondrial presequence of human cytochrome c
oxidase subunit VIII (Rizutto et al., 1989)"
/label=COX8 presequence
/translation="SVLTPLLLRGLTGSARRLPVPRAK"
CDS 3847..5634
/codon_start=1
/label=NeIle
/note="/vntifkey=21"
/translation="MEDIKVAVVGAMSAGADWRISKEACPNGKTLISTLKWSYTTGDGK
RYRSTARTTFTFAKPMAANYLKNQPMYVFRRTELKHSKTEMDFKEWQKAFTDVVGMDEL
YKVDGGSGGTGMVSEGEVDNMASLPATHELHIFGSINGVGFDMVGQGSGNPNVGYEELN
LKSTKGELQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTVRFEDGAS
LTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAFHIGPVTQYGDQEFTGAEQAV
ADINARGGIKGNKLQIVKYDDACDPKQAVAVANRVVNDGIKYVIGHLCSSSAQPASDIY
EDAGILMITPAATAPELTARGYQLILRTTGLDSDQGLTAAKYILEKVKPQRIAIVHDKQ
QYGEGLARAVQGGLKKGNANVVFFDGITAGEKDFSTLVARLKKENIDFVYYGGYHPEMG
QILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYDQVPANKHIVDAVK
AKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDTVMGPLTWDEKGDLK
GFEFGVFDWHANGTATDAK"
misc_feature 5665..6253
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
polyA_signal 6296..6417
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature join(6763,1..940)
/label=ColE1
/note="ColE1"
ORIGIN
1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
1921 aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1981 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
2041 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
2101 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
2161 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
2221 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
2281 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
2341 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccatt
2401 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct
2461 gcgcgctcgc tcgctcactg aggccgcccg ggcaaagccc gggcgtcggg cgacctttgg
2521 tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga gtggccaact ccatcactag
2581 gggttccttg tagttaatga ttaacccgcc atgctactta tctacgtagc catgctctag
2641 gaagatcgta ggttaattaa ggcgcgcccc taggggagat ggccagccag cccattgacg
2701 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
2761 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
2821 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
2881 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
2941 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
3001 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
3061 gggcgcgcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
3121 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
3181 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
3241 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
3301 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
3361 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
3421 ggccctttgt gcggggggag cggctcgggg ctgtccgcgg ggggacggct gccttcgggg
3481 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
3541 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
3601 gctgtctcat cattttggca aagaattgga tccaccggtg acaccatgtc cgtcctgacg
3661 ccgctgctgc tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag
3721 atccattcgt tggggcctgc aagatccagt gtattaacac ccctgctgct gcggggcttg
3781 acaggctcgg cccggcggct cccagtgccg cgcgccaaga ttcacagtct tggtccagct
3841 agatctatgg aagatattaa agtcgcggtc gtgggcgcaa tgtccgctgg tgctgactgg
3901 cgtatatcca aggaggcttg ccccaatggt aagactctta tttccacact taagtggtct
3961 tataccaccg gcgacggcaa acgctaccgg tctactgctc gcaccacatt cacattcgcc
4021 aaaccaatgg ccgcaaatta cctcaaaaat cagccgatgt atgtcttccg gagaacagag
4081 ctgaaacatt ctaagaccga aatggatttt aaagagtggc aaaaggcctt tacggacgtg
4141 gtgggcatgg acgagctgta caaggtggat ggcggcagcg gtggcaccgg catggtctca
4201 gagggagagg tggacaacat ggctagtctg cctgctaccc acgaactgca tatttttggc
4261 tccataaatg gcgtcggttt cgacatggta ggccaaggct ccggtaatcc aaacgtcggt
4321 tatgaggagc tgaacctgaa gtccaccaag ggcgaactgc agttcagtcc ctggatcctg
4381 gtgccgcaca ttgggtatgg gtttcatcag tatcttccct acccggacgg gatgtcgcca
4441 tttcaggctg caatggtcga cgggtccgga tatcaggtgc accgcactgt gcggtttgag
4501 gacggtgctt cactgaccgt gaattacaga tacacctacg aaggatcaca tatcaaaggg
4561 gaagctcagg tgaagggcac tgggttcccg gcagacggcc ctgtgatgac aaattccctg
4621 acagcctttc atattggtcc ggtgacccag tacggtgacc aggagtttac cggcgcagag
4681 caggcggttg cggatatcaa cgctagaggc ggcattaagg gcaacaaact gcaaatcgta
4741 aaatatgacg atgcctgtga cccgaaacag gctgttgcgg tggcgaacag agtcgttaac
4801 gacggcatta aatatgtgat tggtcacctc tgttcttcat cagcgcagcc tgcgtctgac
4861 atctacgaag acgcaggcat tttaatgatc accccagcgg caaccgcgcc ggagctgacc
4921 gcccgtggct atcagctgat cctgcgcacc accggcctgg actccgacca ggggctgacg
4981 gcggcgaaat atattcttga gaaagtgaaa ccgcagcgta ttgctatcgt tcacgacaaa
5041 cagcagtacg gcgaaggtct ggcgcgagcg gtgcagggtg gcctgaagaa aggcaatgca
5101 aacgtggtgt tctttgatgg catcaccgcc ggggaaaaag atttctcaac gctggtggcg
5161 cgtctgaaaa aagagaatat cgacttcgtt tactacggcg gttatcaccc ggaaatgggg
5221 caaatcctgc gtcaggcacg cgcggcaggg ctgaaaactc agtttatggg gccggagggt
5281 gtggctaacg tttcgctgtc taacattgcg ggcgaatcag cggaagggct gctggtgacc
5341 aagccgaaga actacgatca ggttccggcg aacaaacaca ttgttgacgc agtcaaagcg
5401 aaaaaacagg acccaagtgg cgcattcgtt tggaccacct acgccgcgct gcaatctttg
5461 caggcgggcc tgaatcagtc tgacgatccg gctgaaatcg ccaaatacct gaaagcgaac
5521 tccgtggata ccgtaatggg accgctgacc tgggatgaga aaggcgatct gaaaggcttt
5581 gagttcggcg tatttgactg gcacgccaac ggcacggcga ccgatgcgaa gtaaactagt
5641 gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg
5701 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
5761 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
5821 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
5881 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
5941 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
6001 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
6061 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
6121 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
6181 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
6241 gccgcctccc cgcatcgata ccgtcgaccc gggcggccgc ttcgagcaga catgataaga
6301 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
6361 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
6421 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
6481 agcaagtaaa acctctacaa atgtggtaaa atcgataagg atcttcctag agcatggcta
6541 cgtagataag tagcatggcg ggttaatcat taactacaag gaacccctag tgatggagtt
6601 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
6661 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcattaatga
6721 atcggccaac gcgcggggag aggcggtttg cgtattgggc gct
//
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