pSilent-Dual1

LOCUS       pSilient-Dual1                5567 bp ds-DNA     circular SYN 31-AUG-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSilient-Dual1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, August 31, 2016 from SnapGene Viewer 3.1.4
            http://www.biofeng.com/
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /note="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1041..1835
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="NeoR/KanR"
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    2328..2700
                     /note="PtrpC"
     primer_bind     complement(2732..2748)
                     /note="SK primer"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(2757..3360)
                     /note="PgpdA"
     promoter        complement(3379..3397)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3418..3434)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3442..3458
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3466..3496)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    3511..3532
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3820..4408)
                     /direction=LEFT
                     /note="pUC ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4579..5439)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5440..5544)
                     /gene="bla"
                     /note="AmpR promoter"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggacgtta actgatattg aaggagcact ttttgggctt ggctggagct
      721 agtggaggtc aacaatgaat gcctattttg gtttagtcgt ccaggcggtg agcacaaaat
      781 ttgtgtcgtt tgacaagatg gttcatttag gcaactggtc agatcagccc cacttgtagc
      841 agtagcggcg gcgctcgaag tgtgactctt attagcagac aggaacgagg acattattat
      901 catctgctgc ttggtgcacg ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc
      961 cggtcatacc ttcttaagtt cgcccttcct ccctttattt cagattcaat ctgacttacc
     1021 tattctaccc aagcatcgat atgattgaac aagatggatt gcacgcaggt tctccggccg
     1081 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     1141 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     1201 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     1261 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     1321 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     1381 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     1441 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     1501 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     1561 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     1621 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     1681 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     1741 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     1801 tcgccttcta tcgccttctt gacgagttct tctgaggatc cacttaacgt tactgaaatc
     1861 atcaaacagc ttgacgaatc tggatataag atcgttggtg tcgatgtcag ctccggagtt
     1921 gagacaaatg gtgttcagga tctcgataag atacgttcat ttgtccaagc agcaaagagt
     1981 gccttctagt gatttaatag ctccatgtca acaagaataa aacgcgtttc gggtttacct
     2041 cttccagata cagctcatct gcaatgcatt aatgcattgg acctcgcaac cctagtacgc
     2101 ccttcaggct ccggcgaagc agaagaatag cttagcagag tctattttca ttttcgggag
     2161 acgagatcaa gcagatcaac ggtcgtcaag agacctacga gactgaggaa tccgctcttg
     2221 gctccacgcg actatatatt tgtctctaat tgtactttga catgctcctc ttctttactc
     2281 tgatagcttg actatgaaaa ttccgtcacc agccctgggt tctcgaggtc gacgacgtta
     2341 actgagattg aaggagcact ttttgggctt ggctggagct agtggaggtc aacaatgaat
     2401 gcctattttg gtttagtcgt ccaggcggtg agcacaaaat ttgtgtcgtt tgacaagatg
     2461 gttcatttag gcaactggtc agatcagccc cacttgtagc agtagcggcg gcgctcgaag
     2521 tgtgactctt attagcagac aggaacgagg acattattat catctgctgc ttggtgcacg
     2581 ataacttggt gcgtttgtca agcaaggtaa gtgaacgacc cggtcatacc ttcttaagtt
     2641 cgcccttcct ccctttattt cagattcaat ctgacttacc tattctaccc aagcatcgat
     2701 aagcttgata tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgcaaat
     2761 aaaggttctt ggatgggaag atgaatatac tgaagatggg aaaagaaaga gaaaagaaaa
     2821 gagcagctgg tggggagagc aggaaaatat ggcaacaaat gttggactga cgcaacgacc
     2881 ttgtcaaccc cgccgacaca ccgggcggac agacggggca aagctgccta ccagggactg
     2941 agggacctca gcaggtcgag tgcagagcac cggatgggtc gactgccagc ttgtgttccc
     3001 ggtctgcgcc gctggccagc tcctgagcgg cctttccggt ttcatacacc gggcaaagca
     3061 ggagaggcac gatatttgga cgccctacag atgccggatg ggccaattag ggagcttacg
     3121 cgccgggtac tcgctctacc tacttcggag aaggtactat ctcgtgaatc ttttaccaga
     3181 tcggaagcaa ttggacttct gtacctaggt taatggcatg ctatttcgcc gacggctata
     3241 cacccctggc ttcacattct ccttcgctta ctgccggtga ttcgatgaag ctccatattc
     3301 tccgatgatg caatagattc ttggtcaacg aggggcacac cagcctttcc acttcggggc
     3361 gagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc
     3421 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     3481 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     3541 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3601 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3661 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3721 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3781 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3841 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3901 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3961 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4021 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4081 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4141 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4201 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4261 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4321 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4381 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4441 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     4501 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     4561 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     4621 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     4681 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     4741 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     4801 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     4861 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     4921 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     4981 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5041 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5101 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5161 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5221 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     5281 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     5341 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     5401 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     5461 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5521 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccac
//