| 质粒名称: | pHes1(467)-luc |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 41723 |
| 存储实验室: | Ryoichiro Kageyama, Raphael Kopan |
|
载体骨架基本信息 |
|
| 载体名称: | pGL2-Basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5597 bp |
| 完整质粒大小: | 6110 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Hes1 Promoter (-467 to +46),Hes1,hairy and enhancer of split 1 Promoter (-467 to +46),hairy and enhancer of split 1 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 513 bp |
| 基因突变: | 包含小鼠Hes1启动子（-467到+46） |
| GenBank编号 | NC_000082.6 |
| 启动子 | Hes1启动子片段（-467到+46） |
| 标签/融合蛋白: | Firefly luciferase（主干上的C末端） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | F1ori-F (GTGGACTCTTGTTCCAAACTGG) |
| 3' 测序引物 | LucNrev |
|
文献引用方法 |
|
| 材料和方法部分: | pHes1(467)-luc was a gift from Ryoichiro Kageyama & Raphael Kopan (Addgene plasmid # 41723 ; http://n2t.net/addgene:41723 ; RRID:Addgene_41723) |
| 参考文献部分: | Structure, chromosomal locus, and promoter of mouse Hes2 gene, a homologue of Drosophila hairy and Enhancer of split. Nishimura M, Isaka F, Ishibashi M, Tomita K, Tsuda H, Nakanishi S, Kageyama R. Genomics. 1998 Apr 1;49(1):69-75. 10.1006/geno.1998.5213 PubMed 9570950 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
-luc质粒图谱.png)
载体简介
载体序列
LOCUS pHes1(467)-luc 6082 bp ds-DNA circular SYN 26-AUG-2020
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHes1(467)-luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6082)
AUTHORS Nishimura M, Isaka F, Ishibashi M, Tomita K, Tsuda H, Nakanishi S,
Kageyama R
TITLE Structure, chromosomal locus, and promoter of mouse Hes2 gene, a
homologue of Drosophila hairy and Enhancer of split.
JOURNAL Genomics. 1998 Apr 1;49(1):69-75.
PUBMED 9570950
REFERENCE 2 (bases 1 to 6082)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 26, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6082
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..1653
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(67..85)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
intron 1894..1959
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2089..2109
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2534..2668
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2810..2827)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(2981..3569)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3061..3080)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3740..4600)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4363..4382
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4601..4705)
/gene="bla"
/label=AmpR promoter
rep_origin 4732..5187
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4819..4838)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5029..5050
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5377..5498
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatcctct agaggatgga
61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
121 gcttttacag atgcacatat cgaggtgaac atcacgtacg cggaatactt cgaaatgtcc
181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgaacatt
361 tcgcagccta ccgtagtgtt tgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
421 aaaaaattac caataatcca gaaaattatt atcatggatt ctaaaacgga ttaccaggga
481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
541 tttgtaccag agtcctttga tcgtgacaaa acaattgcac tgataatgaa ttcctctgga
601 tctactgggt tacctaaggg tgtggccctt ccgcatagaa ctgcctgcgt cagattctcg
661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
781 cgagtcgtct taatgtatag atttgaagaa gagctgtttt tacgatccct tcaggattac
841 aaaattcaaa gtgcgttgct agtaccaacc ctattttcat tcttcgccaa aagcactctg
901 attgacaaat acgatttatc taatttacac gaaattgctt ctgggggcgc acctctttcg
961 aaagaagtcg gggaagcggt tgcaaaacgc ttccatcttc cagggatacg acaaggatat
1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
1141 acgctgggcg ttaatcagag aggcgaatta tgtgtcagag gacctatgat tatgtccggt
1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
1261 ggagacatag cttactggga cgaagacgaa cacttcttca tagttgaccg cttgaagtct
1321 ttaattaaat acaaaggata tcaggtggcc cccgctgaat tggaatcgat attgttacaa
1381 caccccaaca tcttcgacgc gggcgtggca ggtcttcccg acgatgacgc cggtgaactt
1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
1621 aaggccaaga agggcggaaa gtccaaattg taaaatgtaa ctgtattcag cgatgacgaa
1681 attcttagct attgtaatac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
1741 gttattggtg cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga
1801 atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
1861 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
1921 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
1981 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
2041 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
2101 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
2161 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
2221 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
2281 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
2341 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
2401 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
2461 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
2521 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
2581 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
2641 catcaatgta tcttatcatg tctggatccg tcgaccgatg cccttgagag ccttcaaccc
2701 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
2761 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
2821 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
2881 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
2941 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
3001 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
3061 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
3121 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
3181 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
3241 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
3301 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
3361 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
3421 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
3481 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
3541 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
3601 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
3661 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
3721 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
3781 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
3841 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
3901 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
3961 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
4021 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
4081 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
4141 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
4201 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
4261 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
4321 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
4381 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
4441 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
4501 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
4561 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
4621 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
4681 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct
4741 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4801 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4861 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4921 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
4981 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
5041 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
5101 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
5161 ttaacaaaat attaacgctt acaatttgcc attcgccatt caggctgcgc aactgttggg
5221 aagggcgatc ggtgcgggcc tcttcgctat tacgccagcc caagctacca tgataagtaa
5281 gtaatattaa ggtacgtgga ggttttactt gctttaaaaa acctcccaca cctccccctg
5341 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat
5401 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
5461 tctagttgtg gtttgtccaa actcatcaat gtatcttatg gtactgtaac tgagctaaca
5521 taacccaatt ccggaagaca ttagagtaac acatcctctt taccttgttc cctccttttt
5581 tcaatcacta aattttgtct tggcctatat ctgttcaaaa tatttttcaa atgaacttat
5641 tatacaaagt agttatattg catgcagcaa gaacaataaa aaccaaaggc ctggccacaa
5701 aagaaataga ctagactaaa actaagcaaa gcccagagga aagagttagc aaagggttaa
5761 aatccttttg attgacgttg tagcctccgg tgccccgggc tcaggcgcgc gccattggcc
5821 gccagacctt gtgcctagcg gccaatgggg gggcgcagtc cacgagcggt gccgcgtgtc
5881 tcttcctccc attggctgaa agttactgtg ggaaagaaag tttgggaagt ttcacacgag
5941 ccgttcgcgt gcagtcccag atatatatag aggccgccag ggcctgcgga tcacacagga
6001 tctggagctg gtgctgataa cagcggaatc ccctgtctgg tcgagatcta agtaagcttg
6061 gcattccggt actgttggta aa
//
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