| 载体名称: | pBR327par |
|---|---|
| 载体又名: | pBR327par载体，pBR327par质粒，pBR327par载体质粒 |
| 英文名称: | pBR327par, pBR327par Vector, pBR327par Plasmid |
| 质粒类型: | 大肠杆菌克隆质粒 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 3.7 kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素，四环素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 备注: | |
| 产品目录号: | 217502369 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pBR327par载体质粒基本信息
pBR327par质粒图谱载体图谱和pBR327par载体序列质粒序列多克隆位点信息
pBR327par质粒载体简介
pBR327par质粒序列载体序列
LOCUS pBR327par 3668 bp DNA circular SYN 26-JUL-1993
DEFINITION pBR327par cloning vector (more stable derivative of pBR327).
ACCESSION L08857
VERSION L08857.1
KEYWORDS .
SOURCE synthetic construct
ORGANISM synthetic construct
other sequences; artificial sequences.
REFERENCE 1 (bases 1 to 3668)
AUTHORS Gilbert,W.
TITLE Obtained from VecBase 3.0
JOURNAL Unpublished
COMMENT These data and their annotation were supplied to GenBank by Will
Gilbert under the auspices of the GenBank Currator Program.
pBR327par - Cloning Vector, more stable derivative of pBR327 ENTRY
PBR327PAR #TYPE DNA CIRCULAR TITLE pBR327par -
Cloning Vector, more stable derivative of pBR327 DATE
27-MAR-1986
#sequence 16-DEC-1986
ACCESSION VB0006
SOURCE artificial
REFERENCE
#number 1
#authors Zurita M., Bolivar F. , Soberon X.
#journal Gene (1984) 28: 119-122
#title
Construction and characterization of new cloning vehicles:
VII. Construction of plasmid pBR327par, a completely
sequenced, stable derivatieve of pBR327 containing the par
locus of pSC101
REFERENCE
#number 2
#authors Miller C.A., Tucker W.T., Meacock P.A.,
Gustafsson P., Cohen S.N.
#journal Gene (1983) 24: 309-315
#title
nucleotide sequence of the partition locus of escherichia
coli plasmid psc101
REFERENCE
#number 3
#authors Pouwels P.H., Enger-Valk B.E., Brammar W.J.
#book Cloning Vectors, Elsvier 1985 and supplements
#comment vector I-A-iv-3
COMMENT
Assembled by F. Pfeiffer, MPI, Martinsried
Revised 16-DEC-1986 by F. Pfeiffer:
2036/7 'AT' to 'TA' to match revised sequence of PBR322 COMMENT
Plasmid pBR327par was constructed by inserting a DNA fragment
derived from plasmid pSC101 containing the partition (par)
region
into the AvaI site of plasmid pBR327. The new cloning vehicle
has
all the cloning properties of the parental plasmid, and is more
stable than pBR327.
COMMENT
For the sequences of the pBR327/pSC101 borders see GenBank
entries pBR327p1 and pBR327p2.
KEYWORDS
CROSSREFERENCE
#parent
VecBase(3):pBR327,
GenBank(50):pBR327p1, GenBank(50):pBR327p2,
GenBank(50):pSC101C, GenBank(50):M12801
PARENT
Features of pBR327par (3668 bp)
residue source
1-1430 1-1430 pBR327
1445-1812 4524-4893 pSC101 (complete) (GenBank(50):pSC101C)
1445-1811 1- 373 pSC101par (GenBank(50):M12801)
1817-3668 1423-3274 pBR327
Conflict (cfl) and Mutations (mut):
pBR327par source
cfl 1568-69 C-G CTG 4047-49 pSC101 (complete)
cfl 1624-25 G-A GAA 4704-06 pSC101 (complete)
FEATURE
86-1276 1-1191 Tc-R; tetracycline resistance protein
2603-3391 789- 1 (c) Ap-R; b-lactamase
POLYLINKER none
SELECTION #resistance Ap, Tc
SUMMARY pBR327par #length 3668 #checksum 2179.
FEATURES Location/Qualifiers
source 1..3668
/organism="synthetic construct"
/mol_type="genomic DNA"
/db_xref="taxon:32630"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
421 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
481 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
541 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
601 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
661 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
721 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
781 ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
841 tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
901 acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
961 cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
1021 cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
1081 tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
1141 gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
1201 tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
1261 ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
1321 attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
1381 atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg atccgtcagc
1441 ttgggacagt aagacgggta agcctgttga tgataccgct gccttactgg gtgcattagc
1501 cagtctgaat gacctgtcac gggataatcc gaagtggtca gactggaaaa tcagagggca
1561 ggaactgcga acagcaaaaa gtcagatagc accacatagc agacccgcca taaaacgccc
1621 tgagagcccg tgacgggctt ttcttgtatt atgggtagtt tccttgcatg aatccataaa
1681 aggcgcctgt agtgccattt acccccattc actgccagag ccgtgagcgc agcgaactga
1741 atgtcacgaa aaagacagcg actcaggtgc ctgatggtcg gagacaaaag gaatattcag
1801 cgatttgccc gaacggatct cgggccgcgt tgctggcgtt tttccatagg ctccgccccc
1861 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
1921 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
1981 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
2041 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
2101 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
2161 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
2221 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2281 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2341 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2401 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2461 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
2521 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
2581 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
2641 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2701 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
2761 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
2821 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
2881 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt
2941 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
3001 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
3061 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
3121 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
3181 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata
3241 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3301 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3361 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3421 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3481 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
3541 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
3601 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
3661 ttcaagaa
//
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