hCas9

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: hCas9 
出品公司: 爱迪基因
目录编号: 41815
存储实验室: George Church
用途: 表达人的密码子优化的Cas9核酸酶的基因工程

载体骨架基本信息
载体名称: pcDNA3.3-TOPO
载体出品公司: Invitrogen
载体抗性: 氨苄霉素
载体类型: 哺乳细胞表达,CRISPR
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Cas9
插入大小: 4140 bp
基因突变: human codon-optimized
启动子 CMV
克隆方法: TOPO Cloning
5' 测序引物 CMV-F

文献引用方法
材料和方法部分: hCas9 was a gift from George Church (Addgene plasmid # 41815)
参考文献部分: RNA-Guided Human Genome Engineering via Cas9. Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM. Science. 2013 Jan 3. 10.1126/science.1232033 PubMed 23287722

载体图谱质粒图谱和多克隆位点信息

hCas9载体图谱

载体简介

载体序列

LOCUS       hCas9 Sequencing Result	9553 bp 	DNA	SYN
DEFINITION  hCas9 Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9553
                     /organism="hCas9 Sequencing Result"
                     /mol_type="other DNA"
     promoter        51..627
                     /label="CMV_immearly_promoter"
     misc_feature    130..417
                     /label="CAG_enhancer"
     misc_feature    584..604
                     /label="CMV_fwd_primer"
     promoter        585..654
                     /label="CMV_promoter"
     CDS             589..4887
                     /label="ORF frame 1"
     promoter        597..716
                     /label="CMV2_promoter"
     misc_feature    628..647
                     /label="pCEP_fwd_primer"
     misc_feature    630..654
                     /label="LNCX_primer"
     CDS             complement(724..1227)
                     /label="ORF frame 2"
     CDS             complement(2152..2670)
                     /label="ORF frame 2"
     CDS             complement(2734..3267)
                     /label="ORF frame 2"
     CDS             complement(4261..4794)
                     /label="ORF frame 2"
     misc_feature    4864..4884
                     /label="SV40 NLS"
     misc_feature    4870..4884
                     /label="NLS"
     terminator      4926..5196
                     /label="TK_PA_terminator"
     rep_origin      5250..5556
                     /label="f1_origin"
     misc_feature    complement(5670..5690)
                     /label="pBABE_3_primer"
     misc_feature    complement(5676..5891)
                     /label="SV40_enhancer"
     promoter        5688..5956
                     /label="SV40_promoter"
     rep_origin      5855..5932
                     /label="SV40_origin"
     misc_feature    5917..5936
                     /label="SV40pro_F_primer"
     CDS             6071..6865
                     /label="ORF frame 2"
     gene            6074..6862
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
     terminator      7044..7163
                     /label="SV40_PA_terminator"
     misc_feature    7132..7151
                     /label="EBV_rev_primer"
     promoter        complement(7207..7225)
                     /label="M13_reverse_primer"
     misc_feature    complement(7224..7246)
                     /label="M13_pUC_rev_primer"
     promoter        complement(7260..7289)
                     /label="lac_promoter"
     gene            complement(8372..9232)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(8372..9232)
                     /label="ORF frame 1"
     promoter        complement(9274..9302)
                     /label="AmpR_promoter"
ORIGIN
    1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA
   61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
  121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
  181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
  241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT
  301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC
  361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
  421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
  481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
  541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
  601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG
  661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG
  721 ACTCTAGAGG ATCGAACCCT TGCCACCATG GACAAGAAGT ACTCCATTGG GCTCGATATC
  781 GGCACAAACA GCGTCGGCTG GGCCGTCATT ACGGACGAGT ACAAGGTGCC GAGCAAAAAA
  841 TTCAAAGTTC TGGGCAATAC CGATCGCCAC AGCATAAAGA AGAACCTCAT TGGCGCCCTC
  901 CTGTTCGACT CCGGGGAGAC GGCCGAAGCC ACGCGGCTCA AAAGAACAGC ACGGCGCAGA
  961 TATACCCGCA GAAAGAATCG GATCTGCTAC CTGCAGGAGA TCTTTAGTAA TGAGATGGCT
 1021 AAGGTGGATG ACTCTTTCTT CCATAGGCTG GAGGAGTCCT TTTTGGTGGA GGAGGATAAA
 1081 AAGCACGAGC GCCACCCAAT CTTTGGCAAT ATCGTGGACG AGGTGGCGTA CCATGAAAAG
 1141 TACCCAACCA TATATCATCT GAGGAAGAAG CTTGTAGACA GTACTGATAA GGCTGACTTG
 1201 CGGTTGATCT ATCTCGCGCT GGCGCATATG ATCAAATTTC GGGGACACTT CCTCATCGAG
 1261 GGGGACCTGA ACCCAGACAA CAGCGATGTC GACAAACTCT TTATCCAACT GGTTCAGACT
 1321 TACAATCAGC TTTTCGAAGA GAACCCGATC AACGCATCCG GAGTTGACGC CAAAGCAATC
 1381 CTGAGCGCTA GGCTGTCCAA ATCCCGGCGG CTCGAAAACC TCATCGCACA GCTCCCTGGG
 1441 GAGAAGAAGA ACGGCCTGTT TGGTAATCTT ATCGCCCTGT CACTCGGGCT GACCCCCAAC
 1501 TTTAAATCTA ACTTCGACCT GGCCGAAGAT GCCAAGCTTC AACTGAGCAA AGACACCTAC
 1561 GATGATGATC TCGACAATCT GCTGGCCCAG ATCGGCGACC AGTACGCAGA CCTTTTTTTG
 1621 GCGGCAAAGA ACCTGTCAGA CGCCATTCTG CTGAGTGATA TTCTGCGAGT GAACACGGAG
 1681 ATCACCAAAG CTCCGCTGAG CGCTAGTATG ATCAAGCGCT ATGATGAGCA CCACCAAGAC
 1741 TTGACTTTGC TGAAGGCCCT TGTCAGACAG CAACTGCCTG AGAAGTACAA GGAAATTTTC
 1801 TTCGATCAGT CTAAAAATGG CTACGCCGGA TACATTGACG GCGGAGCAAG CCAGGAGGAA
 1861 TTTTACAAAT TTATTAAGCC CATCTTGGAA AAAATGGACG GCACCGAGGA GCTGCTGGTA
 1921 AAGCTTAACA GAGAAGATCT GTTGCGCAAA CAGCGCACTT TCGACAATGG AAGCATCCCC
 1981 CACCAGATTC ACCTGGGCGA ACTGCACGCT ATCCTCAGGC GGCAAGAGGA TTTCTACCCC
 2041 TTTTTGAAAG ATAACAGGGA AAAGATTGAG AAAATCCTCA CATTTCGGAT ACCCTACTAT
 2101 GTAGGCCCCC TCGCCCGGGG AAATTCCAGA TTCGCGTGGA TGACTCGCAA ATCAGAAGAG
 2161 ACCATCACTC CCTGGAACTT CGAGGAAGTC GTGGATAAGG GGGCCTCTGC CCAGTCCTTC
 2221 ATCGAAAGGA TGACTAACTT TGATAAAAAT CTGCCTAACG AAAAGGTGCT TCCTAAACAC
 2281 TCTCTGCTGT ACGAGTACTT CACAGTTTAT AACGAGCTCA CCAAGGTCAA ATACGTCACA
 2341 GAAGGGATGA GAAAGCCAGC ATTCCTGTCT GGAGAGCAGA AGAAAGCTAT CGTGGACCTC
 2401 CTCTTCAAGA CGAACCGGAA AGTTACCGTG AAACAGCTCA AAGAAGACTA TTTCAAAAAG
 2461 ATTGAATGTT TCGACTCTGT TGAAATCAGC GGAGTGGAGG ATCGCTTCAA CGCATCCCTG
 2521 GGAACGTATC ACGATCTCCT GAAAATCATT AAAGACAAGG ACTTCCTGGA CAATGAGGAG
 2581 AACGAGGACA TTCTTGAGGA CATTGTCCTC ACCCTTACGT TGTTTGAAGA TAGGGAGATG
 2641 ATTGAAGAAC GCTTGAAAAC TTACGCTCAT CTCTTCGACG ACAAAGTCAT GAAACAGCTC
 2701 AAGAGGCGCC GATATACAGG ATGGGGGCGG CTGTCAAGAA AACTGATCAA TGGGATCCGA
 2761 GACAAGCAGA GTGGAAAGAC AATCCTGGAT TTTCTTAAGT CCGATGGATT TGCCAACCGG
 2821 AACTTCATGC AGTTGATCCA TGATGACTCT CTCACCTTTA AGGAGGACAT CCAGAAAGCA
 2881 CAAGTTTCTG GCCAGGGGGA CAGTCTTCAC GAGCACATCG CTAATCTTGC AGGTAGCCCA
 2941 GCTATCAAAA AGGGAATACT GCAGACCGTT AAGGTCGTGG ATGAACTCGT CAAAGTAATG
 3001 GGAAGGCATA AGCCCGAGAA TATCGTTATC GAGATGGCCC GAGAGAACCA AACTACCCAG
 3061 AAGGGACAGA AGAACAGTAG GGAAAGGATG AAGAGGATTG AAGAGGGTAT AAAAGAACTG
 3121 GGGTCCCAAA TCCTTAAGGA ACACCCAGTT GAAAACACCC AGCTTCAGAA TGAGAAGCTC
 3181 TACCTGTACT ACCTGCAGAA CGGCAGGGAC ATGTACGTGG ATCAGGAACT GGACATCAAT
 3241 CGGCTCTCCG ACTACGACGT GGATCATATC GTGCCCCAGT CTTTTCTCAA AGATGATTCT
 3301 ATTGATAATA AAGTGTTGAC AAGATCCGAT AAAAATAGAG GGAAGAGTGA TAACGTCCCC
 3361 TCAGAAGAAG TTGTCAAGAA AATGAAAAAT TATTGGCGGC AGCTGCTGAA CGCCAAACTG
 3421 ATCACACAAC GGAAGTTCGA TAATCTGACT AAGGCTGAAC GAGGTGGCCT GTCTGAGTTG
 3481 GATAAAGCCG GCTTCATCAA AAGGCAGCTT GTTGAGACAC GCCAGATCAC CAAGCACGTG
 3541 GCCCAAATTC TCGATTCACG CATGAACACC AAGTACGATG AAAATGACAA ACTGATTCGA
 3601 GAGGTGAAAG TTATTACTCT GAAGTCTAAG CTGGTCTCAG ATTTCAGAAA GGACTTTCAG
 3661 TTTTATAAGG TGAGAGAGAT CAACAATTAC CACCATGCGC ATGATGCCTA CCTGAATGCA
 3721 GTGGTAGGCA CTGCACTTAT CAAAAAATAT CCCAAGCTTG AATCTGAATT TGTTTACGGA
 3781 GACTATAAAG TGTACGATGT TAGGAAAATG ATCGCAAAGT CTGAGCAGGA AATAGGCAAG
 3841 GCCACCGCTA AGTACTTCTT TTACAGCAAT ATTATGAATT TTTTCAAGAC CGAGATTACA
 3901 CTGGCCAATG GAGAGATTCG GAAGCGACCA CTTATCGAAA CAAACGGAGA AACAGGAGAA
 3961 ATCGTGTGGG ACAAGGGTAG GGATTTCGCG ACAGTCCGGA AGGTCCTGTC CATGCCGCAG
 4021 GTGAACATCG TTAAAAAGAC CGAAGTACAG ACCGGAGGCT TCTCCAAGGA AAGTATCCTC
 4081 CCGAAAAGGA ACAGCGACAA GCTGATCGCA CGCAAAAAAG ATTGGGACCC CAAGAAATAC
 4141 GGCGGATTCG ATTCTCCTAC AGTCGCTTAC AGTGTACTGG TTGTGGCCAA AGTGGAGAAA
 4201 GGGAAGTCTA AAAAACTCAA AAGCGTCAAG GAACTGCTGG GCATCACAAT CATGGAGCGA
 4261 TCAAGCTTCG AAAAAAACCC CATCGACTTT CTCGAGGCGA AAGGATATAA AGAGGTCAAA
 4321 AAAGACCTCA TCATTAAGCT TCCCAAGTAC TCTCTCTTTG AGCTTGAAAA CGGCCGGAAA
 4381 CGAATGCTCG CTAGTGCGGG CGAGCTGCAG AAAGGTAACG AGCTGGCACT GCCCTCTAAA
 4441 TACGTTAATT TCTTGTATCT GGCCAGCCAC TATGAAAAGC TCAAAGGGTC TCCCGAAGAT
 4501 AATGAGCAGA AGCAGCTGTT CGTGGAACAA CACAAACACT ACCTTGATGA GATCATCGAG
 4561 CAAATAAGCG AATTCTCCAA AAGAGTGATC CTCGCCGACG CTAACCTCGA TAAGGTGCTT
 4621 TCTGCTTACA ATAAGCACAG GGATAAGCCC ATCAGGGAGC AGGCAGAAAA CATTATCCAC
 4681 TTGTTTACTC TGACCAACTT GGGCGCGCCT GCAGCCTTCA AGTACTTCGA CACCACCATA
 4741 GACAGAAAGC GGTACACCTC TACAAAGGAG GTCCTGGACG CCACACTGAT TCATCAGTCA
 4801 ATTACGGGGC TCTATGAAAC AAGAATCGAC CTCTCTCAGC TCGGTGGAGA CAGCAGGGCT
 4861 GACCCCAAGA AGAAGAGGAA GGTGTGAAAG GGTTCGATCC CTACCGGTTA GTAATGAGTT
 4921 TAAACGGGGG AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT
 4981 GACGGCAATA AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG
 5041 GGGTTCGGTC CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT
 5101 ACGCCCGCGT TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC
 5161 TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA
 5221 GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC
 5281 GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT
 5341 CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG
 5401 GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT
 5461 CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT
 5521 TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT
 5581 CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT
 5641 AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC
 5701 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG
 5761 GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA
 5821 GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC
 5881 CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC
 5941 CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG
 6001 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
 6061 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
 6121 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
 6181 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
 6241 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
 6301 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
 6361 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
 6421 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
 6481 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
 6541 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
 6601 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
 6661 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
 6721 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC
 6781 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA
 6841 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG
 6901 CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG
 6961 GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC
 7021 TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA
 7081 ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT
 7141 CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG
 7201 CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA
 7261 ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA
 7321 CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC
 7381 ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT
 7441 CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT
 7501 CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG
 7561 CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA
 7621 GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC
 7681 CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG
 7741 TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC
 7801 TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG
 7861 GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC
 7921 TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA
 7981 TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG
 8041 GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA
 8101 AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG
 8161 TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT
 8221 CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT
 8281 TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT
 8341 AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA
 8401 TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA
 8461 CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC
 8521 GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA
 8581 GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG
 8641 TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG
 8701 TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG
 8761 TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG
 8821 TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC
 8881 TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT
 8941 TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA
 9001 CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA
 9061 AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA
 9121 ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC
 9181 AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC
 9241 TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
 9301 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
 9361 CTGACGTCGA CGGATCGGGA GATCTCCCGA TCCCCTATGG TGCACTCTCA GTACAATCTG
 9421 CTCTGATGCC GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA
 9481 GTAGTGCGCG AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA
 9541 GAATCTGCTT AGG
//

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