PCDH-CMV-mCherry-TRIM22

LOCUS      PCDH-CMV-mCherry-TRIM22           8646 bp DNA     circular SYN 04-SEP-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8646)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8646
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3220..3258
                     /label=TRIM22-F
     primer_bind     3220..3243
                     /label=TRIM20-F
     misc_feature    3240..4736
                     /label=TRIM22
     primer_bind     complement(4718..4755)
                     /label=TRIM22-R
     primer_bind     complement(4733..4755)
                     /label=TRIM20-R
     misc_feature    4743..5330
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5214..5225)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5403..5636
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5708..5829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5848..5983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6021..6037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6045..6061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6069..6099)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6114..6135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6423..7011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7182..8042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8043..8147)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8516..8529)
                     /label=Primer 5
     primer_bind     8621..8637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggatttctc agtaaaggta gacatagaga aggaggtgac ctgccccatc tgcctggagc
     3301 tcctgacaga acctctgagc ctagattgtg gccacagctt ctgccaagcc tgcatcactg
     3361 caaagatcaa ggagtcagtg atcatctcaa gaggggaaag cagctgtcct gtgtgtcaga
     3421 ccagattcca gcctgggaac ctccgaccta atcggcatct ggccaacata gttgagagag
     3481 tcaaagaggt caagatgagc ccacaggagg ggcagaagag agatgtctgt gagcaccatg
     3541 gaaaaaaact ccagatcttc tgtaaggagg atggaaaagt catttgctgg gtttgtgaac
     3601 tgtctcagga acaccaaggt caccaaacat tccgcataaa cgaggtggtc aaggaatgtc
     3661 aggaaaagct gcaggtagcc ctgcagaggc tgataaagga ggatcaagag gctgagaagc
     3721 tggaagatga catcagacaa gagagaaccg cctggaagaa ttatatccag atcgagagac
     3781 agaagattct gaaagggttc aatgaaatga gagtcatctt ggacaatgag gagcagagag
     3841 agctgcaaaa gctggaggaa ggtgaggtga atgtgctgga taacctggca gcagctacag
     3901 accagctggt ccagcagagg caggatgcca gcacgctcat ctcagatctc cagcggaggt
     3961 tgaggggatc gtcagtagag atgctgcagg atgtgattga cgtcatgaaa aggagtgaaa
     4021 gctggacatt gaagaagcca aaatctgttt ccaagaaact aaagagtgta ttccgagtac
     4081 cagatctgag tgggatgctg caagttctta aagagctgac agatgtccag tactactggg
     4141 tggacgtgat gctgaatcca ggcagtgcca cttcgaatgt tgctatttct gtggatcaga
     4201 gacaagtgaa aactgtacgc acctgcacat ttaagaattc aaatccatgt gatttttctg
     4261 cttttggtgt cttcggctgc caatatttct cttcggggaa atattactgg gaagtagatg
     4321 tgtctggaaa gattgcctgg atcctgggcg tacacagtaa aataagtagt ctgaataaaa
     4381 ggaagagctc tgggtttgct tttgatccaa gtgtaaatta ttcaaaagtt tactccagat
     4441 atagacctca atatggctac tgggttatag gattacagaa tacatgtgaa tataatgctt
     4501 ttgaggactc ctcctcttct gatcccaagg ttttgactct ctttatggct gtgcctccct
     4561 gtcgtattgg ggttttccta gactatgagg caggcattgt ctcatttttc aatgtcacaa
     4621 accacggagc actcatctac aagttctctg gatgtcgctt ttctcgacct gcttatccgt
     4681 atttcaatcc ttggaactgc ctagtcccca tgactgtgtg cccaccgagc tcctgaggat
     4741 ccaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     4801 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     4861 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     4921 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     4981 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     5041 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     5101 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     5161 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     5221 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     5281 tccgccttcg ccctcagacg agtcggatct ccctttggcc gcctccccgc cggtaccttt
     5341 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5401 actggaaggg ctaattcact cccaacgaaa ataagatctg ctttttgctt gtactgggtc
     5461 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5521 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5581 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag
     5641 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     5701 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     5761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     5821 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccag ttccgcccat
     5881 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5941 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac
     6001 ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     6061 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     6121 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     6181 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6241 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6301 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6361 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6421 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6481 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6541 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6601 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6661 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6721 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6781 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6841 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6901 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6961 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7021 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7081 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7141 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7201 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7261 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7321 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7381 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7441 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7501 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7561 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7621 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7681 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7741 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7801 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7861 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7921 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7981 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8041 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8101 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8161 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     8221 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     8281 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     8341 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     8401 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     8461 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8521 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8581 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
     8641 aagctg
//