| 质粒名称: | pcDNA3.1-WT-VWF |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 124794 |
| 存储实验室: | Sriram Neelamegham |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 完整质粒大小: | 13893 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素,100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VWF |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 8480 bp |
| 启动子: | CMV |
| 5’测序引物 | taggcgtgtacggtgggaggtct |
|
文献引用方法 |
|
| 材料和方法部分: | pcDNA3.1-WT-VWF was a gift from Sriram Neelamegham (Addgene plasmid # 124794 ; http://n2t.net/addgene:124794 ; RRID:Addgene_124794) |
| 参考文献部分: | von Willebrand factor self-association is regulated by the shear-dependent unfolding of the A2 domain. Zhang C, Kelkar A, Neelamegham S. Blood Adv. 2019 Apr 9;3(7):957-968. doi: 10.1182/bloodadvances.2018030122. 10.1182/bloodadvances.2018030122 PubMed 30936056 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pcDNA3.1-WT-VWF 13893 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses multimeric human VWF with C-terminal c-Myc tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1-WT-VWF
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13893)
AUTHORS Zhang C, Kelkar A, Neelamegham S
TITLE von Willebrand factor self-association is regulated by the
shear-dependent unfolding of the A2 domain.
JOURNAL Blood Adv. 2019 Apr 9;3(7):957-968. doi:
10.1182/bloodadvances.2018030122.
PUBMED 30936056
REFERENCE 2 (bases 1 to 13893)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..13893
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 74..453
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 454..657
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 608..628
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 702..721
/label=T7
/note="T7 promoter, forward primer"
promoter 702..720
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 2384..2395
/codon_start=1
/product="WELQut protease recognition and cleavage site"
/label=WELQut site
/translation="WELQ"
CDS 9264..9293
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
primer_bind complement(9321..9338)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 9327..9551
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9597..10025
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9684..9703)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9894..9915
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(10034..10054)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 10039..10368
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10219..10354
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 10281..10300
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 10435..11229
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(10489..10508)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 11099..11118
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 11405..11526
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(11442..11461)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 11496..11515
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(11575..11591)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11575..11591)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11588..11610)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11599..11615
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11623..11653)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11668..11689
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(11806..11823)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(11977..12565)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(12057..12076)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(12736..13596)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 13359..13378
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(13597..13701)
/gene="bla"
/label=AmpR promoter
primer_bind complement(13776..13795)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
61 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
121 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
181 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
241 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
301 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
361 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
421 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
481 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
541 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
601 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
661 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
721 gagacccaag ctggctagtt aagcttggta ccgagctcgg atccactagt ccagtgtggt
781 ggaattcccg cagccctcat ttgcagggga agatgattcc tgccagattt gccggggtgc
841 tgcttgctct ggccctcatt ttgccaggga ccctttgtgc agaaggaact cgcggcaggt
901 catccacggc ccgatgcagc cttttcggaa gtgacttcgt caacaccttt gatgggagca
961 tgtacagctt tgcgggatac tgcagttacc tcctggcagg gggctgccag aaacgctcct
1021 tctcgattat tggggacttc cagaatggca agagagtgag cctctccgtg tatcttgggg
1081 aattttttga catccatttg tttgtcaatg gtaccgtgac acagggggac caaagagtct
1141 ccatgcccta tgcctccaaa gggctgtatc tagaaactga ggctgggtac tacaagctgt
1201 ccggtgaggc ctatggcttt gtggccagga tcgatggcag cggcaacttt caagtcctgc
1261 tgtcagacag atacttcaac aagacctgcg ggctgtgtgg caactttaac atctttgctg
1321 aagatgactt tatgacccaa gaagggacct tgacctcgga cccttatgac tttgccaact
1381 catgggctct gagcagtgga gaacagtggt gtgaacgggc atctcctccc agcagctcat
1441 gcaacatctc ctctggggaa atgcagaagg gcctgtggga gcagtgccag cttctgaaga
1501 gcacctcggt gtttgcccgc tgccaccctc tggtggaccc cgagcctttt gtggccctgt
1561 gtgagaagac tttgtgtgag tgtgctgggg ggctggagtg cgcctgccct gccctcctgg
1621 agtacgcccg gacctgtgcc caggagggaa tggtgctgta cggctggacc gaccacagcg
1681 cgtgcagccc agtgtgccct gctggtatgg agtataggca gtgtgtgtcc ccttgcgcca
1741 ggacctgcca gagcctgcac atcaatgaaa tgtgtcagga gcgatgcgtg gatggctgca
1801 gctgccctga gggacagctc ctggatgaag gcctctgcgt ggagagcacc gagtgtccct
1861 gcgtgcattc cggaaagcgc taccctcccg gcacctccct ctctcgagac tgcaacacct
1921 gcatttgccg aaacagccag tggatctgca gcaatgaaga atgtccaggg gagtgccttg
1981 tcacaggtca atcacacttc aagagctttg acaacagata cttcaccttc agtgggatct
2041 gccagtacct gctggcccgg gattgccagg accactcctt ctccattgtc attgagactg
2101 tccagtgtgc tgatgaccgc gacgctgtgt gcacccgctc cgtcaccgtc cggctgcctg
2161 gcctgcacaa cagccttgtg aaactgaagc atggggcagg agttgccatg gatggccagg
2221 acgtccagct ccccctcctg aaaggtgacc tccgcatcca gcatacagtg acggcctccg
2281 tgcgcctcag ctacggggag gacctgcaga tggactggga tggccgcggg aggctgctgg
2341 tgaagctgtc ccccgtctat gccgggaaga cctgcggcct gtgtgggaat tacaatggca
2401 accagggcga cgacttcctt accccctctg ggctggcgga gccccgggtg gaggacttcg
2461 ggaacgcctg gaagctgcac ggggactgcc aggacctgca gaagcagcac agcgatccct
2521 gcgccctcaa cccgcgcatg accaggttct ccgaggaggc gtgcgcggtc ctgacgtccc
2581 ccacattcga ggcctgccat cgtgccgtca gcccgctgcc ctacctgcgg aactgccgct
2641 acgacgtgtg ctcctgctcg gacggccgcg agtgcctgtg cggcgccctg gccagctatg
2701 ccgcggcctg cgcggggaga ggcgtgcgcg tcgcgtggcg cgagccaggc cgctgtgagc
2761 tgaactgccc gaaaggccag gtgtacctgc agtgcgggac cccctgcaac ctgacctgcc
2821 gctctctctc ttacccggat gaggaatgca atgaggcctg cctggagggc tgcttctgcc
2881 ccccagggct ctacatggat gagagggggg actgcgtgcc caaggcccag tgcccctgtt
2941 actatgacgg tgagatcttc cagccagaag acatcttctc agaccatcac accatgtgct
3001 actgtgagga tggcttcatg cactgtacca tgagtggagt ccccggaagc ttgctgcctg
3061 acgctgtcct cagcagtccc ctgtctcatc gcagcaaaag gagcctatcc tgtcggcccc
3121 ccatggtcaa gctggtgtgt cccgctgaca acctgcgggc tgaagggctc gagtgtacca
3181 aaacgtgcca gaactatgac ctggagtgca tgagcatggg ctgtgtctct ggctgcctct
3241 gccccccggg catggtccgg catgagaaca gatgtgtggc cctggaaagg tgtccctgct
3301 tccatcaggg caaggagtat gcccctggag aaacagtgaa gattggctgc aacacttgtg
3361 tctgtcggga ccggaagtgg aactgcacag accatgtgtg tgatgccacg tgctccacga
3421 tcggcatggc ccactacctc accttcgacg ggctcaaata cctgttcccc ggggagtgcc
3481 agtacgttct ggtgcaggat tactgcggca gtaaccctgg gacctttcgg atcctagtgg
3541 ggaataaggg atgcagccac ccctcagtga aatgcaagaa acgggtcacc atcctggtgg
3601 agggaggaga gattgagctg tttgacgggg aggtgaatgt gaagaggccc atgaaggatg
3661 agactcactt tgaggtggtg gagtctggcc ggtacatcat tctgctgctg ggcaaagccc
3721 tctccgtggt ctgggaccgc cacctgagca tctccgtggt cctgaagcag acataccagg
3781 agaaagtgtg tggcctgtgt gggaattttg atggcatcca gaacaatgac ctcaccagca
3841 gcaacctcca agtggaggaa gaccctgtgg actttgggaa ctcctggaaa gtgagctcgc
3901 agtgtgctga caccagaaaa gtgcctctgg actcatcccc tgccacctgc cataacaaca
3961 tcatgaagca gacgatggtg gattcctcct gtagaatcct taccagtgac gtcttccagg
4021 actgcaacaa gctggtggac cccgagccat atctggatgt ctgcatttac gacacctgct
4081 cctgtgagtc cattggggac tgcgcctgct tctgcgacac cattgctgcc tatgcccacg
4141 tgtgtgccca gcatggcaag gtggtgacct ggaggacggc cacattgtgc ccccagagct
4201 gcgaggagag gaatctccgg gagaacgggt atgagtgtga gtggcgctat aacagctgtg
4261 cacctgcctg tcaagtcacg tgtcagcacc ctgagccact ggcctgccct gtgcagtgtg
4321 tggagggctg ccatgcccac tgccctccag ggaaaatcct ggatgagctt ttgcagacct
4381 gcgttgaccc tgaagactgt ccagtgtgtg aggtggctgg ccggcgtttt gcctcaggaa
4441 agaaagtcac cttgaatccc agtgaccctg agcactgcca gatttgccac tgtgatgttg
4501 tcaacctcac ctgtgaagcc tgccaggagc cgggaggcct ggtggtgcct cccacagatg
4561 ccccggtgag ccccaccact ctgtatgtgg aggacatctc ggaaccgccg ttgcacgatt
4621 tctactgcag caggctactg gacctggtct tcctgctgga tggctcctcc aggctgtccg
4681 aggctgagtt tgaagtgctg aaggcctttg tggtggacat gatggagcgg ctgcgcatct
4741 cccagaagtg ggtccgcgtg gccgtggtgg agtaccacga cggctcccac gcctacatcg
4801 ggctcaagga ccggaagcga ccgtcagagc tgcggcgcat tgccagccag gtgaagtatg
4861 cgggcagcca ggtggcctcc accagcgagg tcttgaaata cacactgttc caaatcttca
4921 gcaagatcga ccgccctgaa gcctcccgca tcgccctgct cctgatggcc agccaggagc
4981 cccaacggat gtcccggaac tttgtccgct acgtccaggg cctgaagaag aagaaggtca
5041 ttgtgatccc ggtgggcatt gggccccatg ccaacctcaa gcagatccgc ctcatcgaga
5101 agcaggcccc tgagaacaag gccttcgtgc tgagcagtgt ggatgagctg gagcagcaaa
5161 gggacgagat cgttagctac ctctgtgacc ttgcccctga agcccctcct cctactctgc
5221 ccccccacat ggcacaagtc actgtgggcc cggggctctt gggggtttcg accctggggc
5281 ccaagaggaa ctccatggtt ctggatgtgg cgttcgtcct ggaaggatcg gacaaaattg
5341 gtgaagccga cttcaacagg agcaaggagt tcatggagga ggtgattcag cggatggatg
5401 tgggccagga cagcatccac gtcacggtgc tgcagtactc ctacatggtg accgtggagt
5461 accccttcag cgaggcacag tccaaagggg acatcctgca gcgggtgcga gagatccgct
5521 accagggcgg caacaggacc aacactgggc tggccctgcg gtacctctct gaccacagct
5581 tcttggtcag ccagggtgac cgggagcagg cgcccaacct ggtctacatg gtcaccggaa
5641 atcctgcctc tgatgagatc aagaggctgc ctggagacat ccaggtggtg cccattggag
5701 tgggccctaa tgccaacgtg caggagctgg agaggattgg ctggcccaat gcccctatcc
5761 tcatccagga ctttgagacg ctcccccgag aggctcctga cctggtgctg cagaggtgct
5821 gctccggaga ggggctgcag atccccaccc tctcccctgc acctgactgc agccagcccc
5881 tggacgtgat ccttctcctg gatggctcct ccagtttccc agcttcttat tttgatgaaa
5941 tgaagagttt cgccaaggct ttcatttcaa aagccaatat agggcctcgt ctcactcagg
6001 tgtcagtgct gcagtatgga agcatcacca ccattgacgt gccatggaac gtggtcccgg
6061 agaaagccca tttgctgagc cttgtggacg tcatgcagcg ggagggaggc cccagccaaa
6121 tcggggatgc cttgggcttt gctgtgcgat acttgacttc agaaatgcat ggtgccaggc
6181 cgggagcctc aaaggcggtg gtcatcctgg tcacggacgt ctctgtggat tcagtggatg
6241 cagcagctga tgccgccagg tccaacagag tgacagtgtt ccctattgga attggagatc
6301 gctacgatgc agcccagcta cggatcttgg caggcccagc aggcgactcc aacgtggtga
6361 agctccagcg aatcgaagac ctccctacca tggtcacctt gggcaattcc ttcctccaca
6421 aactgtgctc tggatttgtt aggatttgca tggatgagga tgggaatgag aagaggcccg
6481 gggacgtctg gaccttgcca gaccagtgcc acaccgtgac ttgccagcca gatggccaga
6541 ccttgctgaa gagtcatcgg gtcaactgtg accgggggct gaggccttcg tgccctaaca
6601 gccagtcccc tgttaaagtg gaagagacct gtggctgccg ctggacctgc ccctgcgtgt
6661 gcacaggcag ctccactcgg cacatcgtga cctttgatgg gcagaatttc aagctgactg
6721 gcagctgttc ttatgtccta tttcaaaaca aggagcagga cctggaggtg attctccata
6781 atggtgcctg cagccctgga gcaaggcagg gctgcatgaa atccatcgag gtgaagcaca
6841 gtgccctctc cgtcgagctg cacagtgaca tggaggtgac ggtgaatggg agactggtct
6901 ctgttcctta cgtgggtggg aacatggaag tcaacgttta tggtgccatc atgcatgagg
6961 tcagattcaa tcaccttggt cacatcttca cattcactcc acaaaacaat gagttccaac
7021 tgcagctcag ccccaagact tttgcttcaa agacgtatgg tctgtgtggg atctgtgatg
7081 agaacggagc caatgacttc atgctgaggg atggcacagt caccacagac tggaaaacac
7141 ttgttcagga atggactgtg cagcggccag ggcagacgtg ccagcccatc ctggaggagc
7201 agtgtcttgt ccccgacagc tcccactgcc aggtcctcct cttaccactg tttgctgaat
7261 gccacaaggt cctggctcca gccacattct atgccatctg ccagcaggac agttgccacc
7321 aggagcaagt gtgtgaggtg atcgcctctt atgcccacct ctgtcggacc aacggggtct
7381 gcgttgactg gaggacacct gatttctgtg ctatgtcatg cccaccatct ctggtctaca
7441 accactgtga gcatggctgt ccccggcact gtgatggcaa cgtgagctcc tgtggggacc
7501 atccctccga aggctgtttc tgccctccag ataaagtcat gttggaaggc agctgtgtcc
7561 ctgaagaggc ctgcactcag tgcattggtg aggatggagt ccagcaccag ttcctggaag
7621 cctgggtccc ggaccaccag ccctgtcaga tctgcacatg cctcagcggg cggaaggtca
7681 actgcacaac gcagccctgc cccacggcca aagctcccac gtgtggcctg tgtgaagtag
7741 cccgcctccg ccagaatgca gaccagtgct gccccgagta tgagtgtgtg tgtgacccag
7801 tgagctgtga cctgccccca gtgcctcact gtgaacgtgg cctccagccc acactgacca
7861 accctggcga gtgcagaccc aacttcacct gcgcctgcag gaaggaggag tgcaaaagag
7921 tgtccccacc ctcctgcccc ccgcaccgtt tgcccaccct tcggaagacc cagtgctgtg
7981 atgagtatga gtgtgcctgc aactgtgtca actccacagt gagctgtccc cttgggtact
8041 tggcctcaac cgccaccaat gactgtggct gtaccacaac cacctgcctt cccgacaagg
8101 tgtgtgtcca ccgaagcacc atctaccctg tgggccagtt ctgggaggag ggctgcgatg
8161 tgtgcacctg caccgacatg gaggatgccg tgatgggcct ccgcgtggcc cagtgctccc
8221 agaagccctg tgaggacagc tgtcggtcgg gcttcactta cgttctgcat gaaggcgagt
8281 gctgtggaag gtgcctgcca tctgcctgtg aggtggtgac tggctcaccg cggggggact
8341 cccagtcttc ctggaagagt gtcggctccc agtgggcctc cccggagaac ccctgcctca
8401 tcaatgagtg tgtccgagtg aaggaggagg tctttataca acaaaggaac gtctcctgcc
8461 cccagctgga ggtccctgtc tgcccctcgg gctttcagct gagctgtaag acctcagcgt
8521 gctgcccaag ctgtcgctgt gagcgcatgg aggcctgcat gctcaatggc actgtcattg
8581 ggcccgggaa gactgtgatg atcgatgtgt gcacgacctg ccgctgcatg gtgcaggtgg
8641 gggtcatctc tggattcaag ctggagtgca ggaagaccac ctgcaacccc tgccccctgg
8701 gttacaagga agaaaataac acaggtgaat gttgtgggag atgtttgcct acggcttgca
8761 ccattcagct aagaggagga cagatcatga cactgaagcg tgatgagacg ctccaggatg
8821 gctgtgatac tcacttctgc aaggtcaatg agagaggaga gtacttctgg gagaagaggg
8881 tcacaggctg cccacccttt gatgaacaca agtgtctggc tgagggaggt aaaattatga
8941 aaattccagg cacctgctgt gacacatgtg aggagcctga gtgcaacgac atcactgcca
9001 ggctgcagta tgtcaaggtg ggaagctgta agtctgaagt agaggtggat attcactact
9061 gccagggcaa atgtgccagc aaagccatgt actccattga catcaacgat gtgcaggacc
9121 agtgctcctg ctgctctccg acacggacgg agcccatgca ggtggccctg cactgcacca
9181 atggctctgt tgtgtaccat gaggttctca atgccatgga gtgcaaatgc tcccccagga
9241 agtgcagcaa ggatatcgct agcgaacaga agctaataag tgaagaggac ctaatctgag
9301 aattcaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
9361 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
9421 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
9481 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
9541 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
9601 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
9661 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
9721 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
9781 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
9841 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
9901 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
9961 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
10021 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
10081 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
10141 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
10201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
10261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
10321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
10381 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
10441 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
10501 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
10561 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
10621 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
10681 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
10741 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
10801 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
10861 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
10921 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
10981 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
11041 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
11101 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
11161 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
11221 ttcttctgag cgggactctg gggttcgcga aatgaccgac caagcgacgc ccaacctgcc
11281 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
11341 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
11401 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
11461 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
11521 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
11581 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
11641 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
11701 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
11761 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
11821 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
11881 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
11941 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
12001 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
12061 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
12121 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
12181 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
12241 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
12301 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
12361 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
12421 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
12481 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
12541 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
12601 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
12661 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
12721 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
12781 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
12841 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
12901 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
12961 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
13021 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
13081 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
13141 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
13201 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
13261 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
13321 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
13381 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
13441 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
13501 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
13561 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
13621 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
13681 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
13741 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
13801 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
13861 atttaagcta caacaaggca aggcttgacc gac
//
