ND6-L1397-N_UGI-NES_right

LOCUS       ND6-L1397-N_UGI-NES_right               5447 bp DNA     circular SYN 02-APR-2026
DEFINITION  expresses the right-side half ND6-UGI-NES in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    ND6-L1397-N_UGI-NES_right
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Lei Z, Meng H, Liu L, Zhao H, Rao X, Yan Y, Wu H, Liu M, He A, Yi C
  TITLE     Mitochondrial base editor induces substantial nuclear off-target 
            mutations.
  JOURNAL   Nature. 2022 May 12. pii: 10.1038/s41586-022-04836-5. doi: 
            10.1038/s41586-022-04836-5.
  PUBMED    35551512
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2022 May 12. pii: 10.1038/s41586-022-04836-5. doi: 
            10.1038/s41586-022-04836-5."
FEATURES             Location/Qualifiers
     source          1..5447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     377..396
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        377..395
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..486
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MASVLTPLLLRGLTGSARRLPVPRAK"
     CDS             499..564
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1987..2235
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             2248..2274
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(2502..2519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2508..2732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2803..2819)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2803..2819)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2816..2838)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2827..2843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2851..2881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2896..2917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    3023..3963
                     /label=ColE1
                     /note="ColE1"
     primer_bind     complement(3034..3051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3205..3793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3285..3304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3964..4824)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4587..4606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4825..4929)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5008..5027)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(5199..5447,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat ggccagcgtg
      421 ctcacaccac tgctgctgag aggcctgaca ggaagcgcgc ggagactgcc tgtccccaga
      481 gccaagatcc acagcctgga ttataaggac cacgacggag attacaaaga ccacgatatc
      541 gactacaagg atgacgacga taagatggac atcgcggacc tcaggacgct gggctacagc
      601 cagcaacagc aagagaagat caagcccaaa gtaaggagca ccgtggccca gcaccacgag
      661 gccctggtgg gtcacggctt cacccacgcg catatcgttg ctctgagcca acatcccgca
      721 gctctgggta ccgttgcggt gaagtatcag gacatgatcg cggcactgcc tgaagctaca
      781 cacgaagcca tagtgggcgt tggcaagaga ggtgctggtg ccagagcgct tgaggcactg
      841 ttgacggtgg ctggcgagct gaggggaccg ccactgcaac tggacaccgg ccaactgctg
      901 aagatcgcca agaggggagg cgtgacggcg gtggaggccg tgcatgcctg gaggaacgca
      961 ctgaccggcg cgcccctgaa cctgacacca gagcaagtag tggctattgc aagtaacaac
     1021 ggtggcaaac aagcgctgga gaccgtgcag aggctccttc cggtgctctg ccaagcacac
     1081 ggtctcactc cggaacaggt ggtcgcaatc gcgagcaaca tcggcggcaa gcaagccctt
     1141 gagacagtcc aaagactttt gcctgtcctt tgtcaggcgc atggccttac gcctgagcaa
     1201 gtcgttgcga tcgcctccaa cggcggcgga aaacaggctt tggaaaccgt gcagcggttg
     1261 ctgcccgttt tgtgccaagc ccacggattg acccccgaac aggttgtagc catagcttct
     1321 aacaacggag gtaagcaggc actggaaacc gtgcagcgcc tgctcccagt actgtgtcag
     1381 gctcatgggc tgacaccaga gcaagtagtg gctattgcaa gtaacaacgg tggcaaacaa
     1441 gcgctggaga ccgtgcagag gctccttccg gtgctctgcc aagcacacgg tctcactccg
     1501 gaacaggtgg tcgcaatcgc gagccacgac ggcggcaagc aagcccttga gacagtccaa
     1561 agacttttgc ctgtcctttg tcaggcgcat ggccttacgc ctgagcaagt cgttgcgatc
     1621 gcctccaacg gcggcggaaa acaggctttg gaaaccgtgc agcggttgct gcccgttttg
     1681 tgccaagccc acggattgac ccccgaacag gttgtagcca tagcttctaa cggcggaggt
     1741 agacctgcac tggaaagcat cgtggcccag ctgagcagac ccgaccctgc cctcgccgct
     1801 ctgaccaacg accacctggt ggccctggct tgcctcgggg gcaggccagc tcttgacgcc
     1861 gtgaagaagg gccttggcgg atccgccatc cccgtgaagc ggggcgctac aggcgagaca
     1921 aaagttttca ccggcaactc caacagcccc aagtccccaa ccaagggcgg ttgtagcggc
     1981 ggcagcacca acctgagcga catcatcgag aaggaaaccg gcaagcagct ggtgatccag
     2041 gagagcatcc tgatgctgcc tgaagaggtg gaagaagtga tcggcaacaa gcctgagagc
     2101 gacatcctgg tgcacaccgc ctacgacgag tctaccgacg agaacgtgat gctgctgaca
     2161 agcgatgccc ctgagtacaa gccctgggcc ctggtgatcc aagactccaa tggcgagaac
     2221 aagatcaaga tgctgggatc tctgcagctg cctccacttg aaagactgac actgtaatag
     2281 accacgatcg ttatgctgat cataccctaa tgatcccagc aagataatgt cctgtcttct
     2341 aagatgtgca tcaagcctgg tacatactga aaaccctata aggtcctgga taatttttgt
     2401 ttgattattc attgaagaaa catttatttt ccaattgtgt gaagtttttg actgttaata
     2461 aaagaatctg tcaaccatca aagtttaaac ccgctgatca gcctcgactg tgccttctag
     2521 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2581 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2641 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2701 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     2761 ctcaataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     2821 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2881 agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2941 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     3001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     3061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     3121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     3181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     3241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     3301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     3661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacactc agtggaacga
     3841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     4021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     4081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     4141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     4201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     4261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     4321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4921 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
     4981 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
     5041 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
     5101 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
     5161 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
     5221 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     5281 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     5341 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     5401 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//