pMSP3545-dCas9Str

LOCUS       pMSP3545-dCas9Str               12621 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expresses dCas9str under nisin-inducible nisA promoter in pMSP3545 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    pMSP3545-dCas9Str
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Afonina I, Ong J, Chua J, Lu T,  Kline KA
  TITLE     Multiplex CRISPRi System Enables the Study of Stage-Specific Biofilm
            Genetic Requirements in Enterococcus faecalis
  JOURNAL   mBio. 2020
REFERENCE   2  (bases 1 to 12621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      7090..7678
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7579..7598
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7832..7849
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     7936..7955
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7936..7954
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(8318..12424)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 agatctgatc cgtagcggtt ttcaaaattt gcaaccagga atgaattact atccctttta
       61 tcaagaagcg cacaaaaaga aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata
      121 atgggagata agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca
      181 gcaaagaatg gcggaaacgt aaaagaagtt atggaaataa gacttagaag caaacttaag
      241 agtgtgttga tagtgcagta tcttaaaatt ttgtataata ggaattgaag ttaaattaga
      301 tgctaaaaat ttgtaattaa gaaggagtga ttacatgaac aaaaatataa aatattctca
      361 aaacttttta acgagtgaaa aagtactcaa ccaaataata aaacaattga atttaaaaga
      421 aaccgatacc gtttacgaaa ttggaacagg taaagggcat ttaacgacga aactggctaa
      481 aataagtaaa caggtaacgt ctattgaatt agacagtcat ctattcaact tatcgtcaga
      541 aaaattaaaa ctgaatactc gtgtcacttt aattcaccaa gatattctac agtttcaatt
      601 ccctaacaaa cagaggtata aaattgttgg gagtattcct taccatttaa gcacacaaat
      661 tattaaaaaa gtggtttttg aaagccatgc gtctgacatc tatctgattg ttgaagaagg
      721 attctacaag cgtaccttgg atattcaccg aacactaggg ttgctcttgc acactcaagt
      781 ctcgattcag caattgctta agctgccagc ggaatgcttt catcctaaac caaaagtaaa
      841 cagtgtctta ataaaactta cccgccatac cacagatgtt ccagataaat attggaagct
      901 atatacgtac tttgtttcaa aatgggtcaa tcgagaatat cgtcaactgt ttactaaaaa
      961 tcagtttcat caagcaatga aacacgccaa agtaaacaat ttaagtaccg ttacttatga
     1021 gcaagtattg tctattttta atagttatct attatttaac gggaggaaat aattctatga
     1081 gtcgcttttg taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag
     1141 gtatactact gacagcttcc aaggagctaa agaggtccct agcgcttaga atcgctttag
     1201 gaaacacgat ccagtccaat aatcgtcgat aaaaactttt gaaaaaggtt ggtgaaatta
     1261 cctacttttg gaataatcac aaatcacaag tgattaatca caaatcacaa gtgattaatc
     1321 acttgtttat taagatatta aaagctataa tttaaataaa gcgtgaattt tattacacaa
     1381 aaagaggggg gagaaacttg gaactagcat ttagagaaag cttaaaaaag atgagaggta
     1441 ccaaatcaaa agaaaaattc tcccaagaat tagaaatgag tagatcaaat tattcacgaa
     1501 tagaatcagg aaaatcagat ccaaccataa aaacactaga acaaattgca aagttaacta
     1561 actcaacgct agtagtggat ttaatcccaa atgagccaac agaaccagaa ccagaaacag
     1621 aatcagaaca agtaacattg gatttagaaa tggaagaaga aaaaagcaat gacttcgtgt
     1681 gaataatgca cgaaatcgtt gcttattttt tttaaaagcg gtatactaga tataacgaaa
     1741 caacgaactg aatagaaacg aaaaaagagc catgacacat ttataaaatg tttgacgaca
     1801 ttttataaat gcatagcccg ataagattgc caaaccaacg cttatcagtt agtcagatga
     1861 actcttccct cgtaagaagt tatttaatta actttgtttg aagacggtat ataaccgtac
     1921 tatcattata tagggaaatc agagagtttt caagtatcta agctactgaa tttaagaatt
     1981 gttaagcaat caatcggaaa tcgtttgatt gctttttttg tattcattta tagaaggtgg
     2041 agtttgtatg aatcatgatg aatgtaaaac ttatataaaa aatagtttat tggagataag
     2101 aaaattagca aatatctata cactagaaac gtttaagaaa gagttagaaa agagaaatat
     2161 ctacttagaa acaaaatcag ataagtattt ttcttcggag ggggaagatt atatatataa
     2221 gttaatagaa aataacaaaa taatttattc gattagtgga aaaaaattga cttataaagg
     2281 aaaaaaatct ttttcaaaac atgcaatatt gaaacagttg aatgaaaaag caaaccaagt
     2341 taattaaaca acctatttta taggatttat aggaaaggag aacagctgaa tgaatatccc
     2401 ttttgttgta gaaactgtgc ttcatgacgg cttgttaaag tacaaattta aaaatagtaa
     2461 aattcgctca atcactacca agccaggtaa aagcaaaggg gccatttttg cgtatcgctc
     2521 aaaatcaagc atgattggcg gtcgtggtgt tgttctgact tccgaggaag cgattcaaga
     2581 aaatcaagat acatttacac attggacacc caacgtttat cgttatggaa cgtatgcaga
     2641 cgaaaaccgt tcatacacga aaggacattc tgaaaacaat ttaagacaaa tcaatacctt
     2701 ctttattgat tttgatattc acacggcaaa agaaactatt tcagcaagcg atattttaac
     2761 aaccgctatt gatttaggtt ttatgcctac tatgattatc aaatctgata aaggttatca
     2821 agcatatttt gttttagaaa cgccagtcta tgtgacttca aaatcagaat ttaaatctgt
     2881 caaagcagcc aaaataattt cgcaaaatat ccgagaatat tttggaaagt ctttgccagt
     2941 tgatctaacg tgtaatcatt ttggtattgc tcgcatacca agaacggaca atgtagaatt
     3001 ttttgatcct aattaccgtt attctttcaa agaatggcaa gattggtctt tcaaacaaac
     3061 agataataag ggccttactc gttcaagtct aacggtttta agcggtacag aaggcaaaaa
     3121 acaagtagat gaaccctggt ttaatctctt attgcacgaa acgaaatttt caggagaaaa
     3181 gggtttaata gggcgtaata acgtcatgtt taccctctct ttagcctact ttagttcagg
     3241 ctattcaatc gaaacgtgcg aatataatat gtttgagttt aataatcgat tagatcaacc
     3301 cttagaagaa aaagaagtaa tcaaaattgt tagaagtgcc tattcagaaa actatcaagg
     3361 ggctaatagg gaatacatta ccattctttg caaagcttgg gtatcaagtg atttaaccag
     3421 taaagattta tttgtccgtc aagggtggtt taaattcaag aaaaaaagaa gcgaacgtca
     3481 acgtgttcat ttgtcagaat ggaaagaaga tttaatggct tatattagcg aaaaaagcga
     3541 tgtatacaag ccttatttag tgacgaccaa aaaagagatt agagaagtgc taggcattcc
     3601 tgaacggaca ttagataaat tgctgaaggt actgaaggcg aatcaggaaa ttttctttaa
     3661 gatcaaacca ggaagaaatg gtggcattca acttgctagt gttaaatcat tgttgctatc
     3721 gatcattaaa gtaaaaaaag aagaaaaaga aagctatata aaggcgctga caaattcttt
     3781 tgacttagag catacattca ttcaagagac tttaaacaag ctagcagaac gccctaaaac
     3841 ggacacacaa ctcgatttgt ttagctatga tacaggctga aaataaaacc cgcactatgc
     3901 cattacattt atatctatga tacgtgtttg ttttttcttt gctgtttagc gaatgattag
     3961 cagaaatata cagagtaaga ttttaattaa ttattagggg gagaaggaga gagtagcccg
     4021 gaaactttta gttggcttgg actgaacgaa gtgagggaaa ggctactaaa acgtcgaggg
     4081 gcagtgagag cgaagcgaac acttgatttt ttaattttct atcttttata ggtcattaga
     4141 gtatacttat ttgtcctata aactatttag cagcataata gatttattga ataggtcatt
     4201 taagttgagc atattagagg aggaaaatct tggagaaata tttgaagaac ccgattacat
     4261 ggattggatt agttcttgtg gttacgtggt ttttaactaa aagtagtgaa tttttgattt
     4321 ttggtgtgtg tgtcttgttg ttagtatttg ctagtcaaag tgattaaata gaattctcat
     4381 gtttgacagc ttatcatcgg agctccgatg ataagctgtc aaacatgaga attcccggct
     4441 ttaggtatag tgtgtatctc aatccttggt atattgaaaa gaaagactaa aaattgatag
     4501 attatatttc ttcagaatga atggtataat gaagtaatga gtactaaaca atcggaggta
     4561 aagtggtgta taaaatttta atagttgatg atgatcagga aattttaaaa ttaatgaaga
     4621 cagcattaga aatgagaaac tatgaagttg cgacgcatca aaacatttca cttcccttgg
     4681 atattactga ttttcaggga tttgatttga ttttgttaga tatcatgatg tcaaatattg
     4741 aagggacaga aatttgtaaa aggattcgca gagaaatatc aactccaatt atctttgtta
     4801 gtgcgaaaga tacagaagag gatattataa acggcttagg tattggtggg gatgactata
     4861 ttactaagcc ttttagcctt aaacagttgg ttgcaaaagt ggaagcaaat ataaagcgag
     4921 aggaacgcaa taaacatgca gttcatgttt tttcagagat tcgtagagat ttaggaccaa
     4981 ttacatttta tttagaagaa aggcgagtct gtgtcaatgg tcaaacaatt ccactgactt
     5041 gtcgcgaata cgatattctt gaattactat cacaacgaac ttctaaagtt tatacgagag
     5101 aggatattta tgatgacgta tatgatgaat attctaatgc actttttcgg tcaatctcgg
     5161 aatatattta tcagattagg agtaagtttg caccatacga tattaatccg ataaaaacgg
     5221 ttcggggact tgggtatcag tggcatgggt aaaaaatatt caatgcgtcg acggatatgg
     5281 caagctgtca ttgaaattat cataggtact tgtctactta tcctgttgtt actgggcttg
     5341 actttctttc tacgacaaat tggacaaatc agtggttcag aaactattcg tttatcttta
     5401 gattcagata atttaactat ttctgatatc gaacgtgata tgaaacacta cccatatgat
     5461 tatattattt ttgacaatga tacaagtaaa attttgggag gacattatgt caagtcggat
     5521 gtacctagtt ttgtagcttc aaaacagtct tcacataata ttacagaagg agaaattact
     5581 tatacttatt caagcaataa gcatttttca gttgttttaa gacaaaacag tatgcctgaa
     5641 tttacaaatc atacgcttcg ttcaatttct tataatcaat ttacttacct tttctttttt
     5701 cttggtgaaa taatactcat tattttttct gtctatcatc tcattagaga attttctaag
     5761 aattttcaag ccgttcaaaa gattgcattg aagatggggg aaataactac ttttcctgaa
     5821 caagaggaat caaaaattat tgaatttgat caggttctga ataacttata ttcgaaaagt
     5881 aaggagttag ctttccttat tgaagcggag cgtcatgaaa aacatgattt atccttccag
     5941 gttgctgcac tttcacatga tgttaagaca cctttaacag tattaaaagg aaatattgaa
     6001 ctgctagaga tgactgaagt aaatgaacaa caagctgatt ttattgagtc aatgaaaaat
     6061 agtttgactg tttttgacaa gtattttaac acaatgatta gttatacaaa acttttgaat
     6121 gatgaaaatg attacaaagc gataatctcc ctggaggatt ttttgataga tttatcagtt
     6181 gagttggaag agttgtcaac aacttatcaa gtggattatc agctagttaa aaaaacagat
     6241 ttaaccactt tttacggaaa tacattagct ttaagtcgag cacttatcaa tatctttgtt
     6301 aatgcctgtc agtatgctaa agagggtgaa aaaatagtcg gtttgagtat ttatgatgat
     6361 gaaaaatatc tctattttga aatctggaat aatggtcatc ctttttctga acaagcaaaa
     6421 aaaaatgctg gaaaactatt tttcacagaa gatactggac gtagtgggaa acactatggg
     6481 attggactat cttttgctca aggtgtagct ttaaaacatc aaggaaactt aattctcagt
     6541 aatcctcaaa aaggtggggc agaagttatc ctaaaaataa aaaagtaatt tagtaatctc
     6601 taaggattac tttttttgtt tcggcaaact attaactggc gaactactta ctctagcttc
     6661 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6721 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6781 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6841 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6901 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6961 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     7021 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     7081 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7141 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7201 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7261 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7321 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7381 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7441 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7501 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7561 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7621 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7681 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7741 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7801 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7861 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggttaac
     7921 ctggcttatc gaaattaata cgactcacta tagggagacc ggcctcgaga agctcgcgtt
     7981 atcggtcctt taattggtgg acaaatttac attagtctcg gacattctgc tcccgccctt
     8041 atgggattta tcttccttat tctcgctttg attgttctat cgaaagcgaa atcaaacgaa
     8101 taatttataa ataaaaaaac cacctctaaa aggtgatttt tatttataaa ttacaatttt
     8161 tcagcaatat cagtaattgc tttatcaact gctgcttttt ggctatcaat caaagcaaca
     8221 cgtgctgtaa tttgtttaat tgccatttca attgaacgtt tcaagccttg gttttctaat
     8281 tttggttcaa agaaagcttg agctctctag aactagttta gtcacctcct agctgactca
     8341 aatcaatgcg tgtttcataa agaccagtga tggattgatg gataagagtg gcatctaaaa
     8401 cttcttttgt agacgtatat cgtttacgat caattgttgt atcaaaatat ttaaaagcag
     8461 cgggagctcc aagattcgtc aacgtaaata aatgaataat attttctgct tgttcacgta
     8521 ttggtttgtc tctatgtttg ttatatgcac taagaacttt atctaaattg gcatctgcta
     8581 aaataacacg cttagaaaat tcactgattt gctcaataat ctcatctaaa taatgcttat
     8641 gctgctccac aaacaattgt ttttgttcgt tatcttctgg actacccttc aacttttcat
     8701 aatgactagc taaatataaa aaattcacat atttgcttgg cagagccagc tcatttcctt
     8761 tttgtaattc tccggcacta gccagcatcc gtttacgacc gttttctaac tcaaaaagac
     8821 tatatttagg tagtttaatg attaagtctt ttttaacttc cttatatcct ttagcttcta
     8881 aaaagtcaat cggatttttt tcaaaggaac ttctttccat aattgtgatc cctagtaact
     8941 ctttaacgga ttttaacttc ttcgatttcc ctttttccac cttagcaacc actaggactg
     9001 aataagctac cgttggacta tcaaaaccac catatttttt tggatcccag tcttttttac
     9061 gagcaataag cttgtccgaa tttctttttg gtaaaattga ctccttggag aatccgcctg
     9121 tctgtacttc tgttttcttg acaatattga cttggggcat ggacaatact ttgcgcactg
     9181 tggcaaaatc tcgcccttta tcccagacaa tttctccagt ttccccatta gtttcgatta
     9241 gagggcgttt gcgaatctct ccatttgcaa gtgtaatttc tgttttgaag aagttcatga
     9301 tattagagta aaagaaatat tttgcggttg ctttgcctat ttcttgctca gacttagcaa
     9361 tcattttacg aacatcataa actttataat caccatagac aaactccgat tcaagttttg
     9421 gatatttctt aatcaaagca gttccaacga cggcatttag atacgcatca tgggcatgat
     9481 ggtaattgtt aatctcacgt actttataga attggaaatc ttttcggaag tcagaaacta
     9541 atttagattt taaggtaatc actttaacct ctcgaataag tttatcattt tcatcgtatt
     9601 tagtattcat gcgactatcc aaaatttgtg ccacatgctt agtgatttgg cgagtttcaa
     9661 ccaattggcg tttgataaaa ccagctttat caagttcact caaacctcca cgttcagctt
     9721 tcgttaaatt atcaaactta cgttgagtga ttaacttggc gtttagaagt tgtctccaat
     9781 agtttttcat ctttttgact acttcttcac ttggaacgtt atccgattta ccacgatttt
     9841 tatcagaacg cgttaagacc ttattgtcta ttgaatcgtc tttaaggaaa ctttgtggaa
     9901 caatggcatc gacatcataa tcacttaaac gattaatatc taattcttgg tccacataca
     9961 tgtctcttcc attttggaga taatagagat agagcttttc attttgcaat tgagtatttt
    10021 caacaggatg ctctttaaga atctgacttc ctaattcttt gataccttct tcgattcgtt
    10081 tcatacgctc tcgcgaattt ttctggccct tttgagttgt ctgattttca cgtgccattt
    10141 caataacgat attttctggc ttatgccgcc ccattacttt gaccaattca tcaacaactt
    10201 ttacagtctg taaaatacct tttttaatag cagggctacc agctaaattt gcaatatgtt
    10261 catgtaaact atcgccttgt ccagacactt gtgctttttg aatgtcttct ttaaatgtca
    10321 aactatcatc atggatcagc tgcataaaat tgcgattggc aaaaccatct gatttcaaaa
    10381 aatctaatat tgttttgcca gattgcttat ccctaatacc attaatcaat tttcgagaca
    10441 aacgtcccca accagtataa cggcgacgtt taagctgttt catcacctta tcatcaaaga
    10501 ggtgagcata tgttttaagt ctttcctcaa tcatctccct atcttcaaat aaggtcaatg
    10561 ttaaaacaat atcctctaag atatcttcat tttcttcatt atccaaaaaa tctttatctt
    10621 taataatttt tagcaaatca tggtaggtac ctaatgaagc attaaatcta tcttcaactc
    10681 ctgaaatttc aacactatca aaacattcta tttttttgaa ataatcttct tttaattgct
    10741 taacggttac ttttcgattt gttttgaaga gtaaatcaac aatggctttc ttctgttcac
    10801 ctgaaagaaa tgctggtttt cgcattcctt cagtaacata tttgaccttt gtcaattcgt
    10861 tataaaccgt aaaatactca taaagcaaac tatgttttgg tagtactttt tcatttggaa
    10921 gatttttatc aaagtttgtc atgcgttcaa taaatgattg agctgaagca cctttatcga
    10981 caacttcttc aaaattccat ggggtaattg tttcttcaga cttccgagtc atccatgcaa
    11041 aacgactatt gccacgcgcc aatggaccaa cataataagg aattcgaaaa gtcaagattt
    11101 tttcaatctt ctcacgattg tcttttaaaa atggataaaa gtcttcttgt cttctcaaaa
    11161 tagcatgcag ctcacccaag tgaatttgat ggggaataga gccgttgtca aaggtccgtt
    11221 gcttgcgcag caaatcttca cgatttagtt tcaccaataa ttcctcagta ccatccattt
    11281 tttctaaaat tggtttgata aatttataaa attcttcttg gctagctccc ccatcaatat
    11341 aacctgcata tccgtttttt gattgatcaa aaaagatttc tttatacttt tctggaagtt
    11401 gttgtcgaac taaagctttt aaaagagtca agtcttgatg atgttcatcg tagcgtttaa
    11461 tcattgaagc tgatagggga gccttagtta tttcagtatt tactcttagg atatctgaaa
    11521 gtaaaatagc atctgataaa ttcttagctg ccaaaaacaa atcagcatat tgatctccaa
    11581 tttgcgccaa taaattatct aaatcatcat cgtaagtatc ttttgaaagc tgtaatttag
    11641 catcttctgc caaatcaaaa tttgatttaa aattaggggt caaacccaat gacaaagcaa
    11701 tgagattccc aaataagcca tttttcttct caccggggag ctgagcaatg agattttcta
    11761 atcgtcttga tttactcaat cgtgcagaaa gaatcgcttt agcatctact ccacttgcgt
    11821 taatagggtt ttcttcaaat aattgattgt aggtttgtac caactggata aatagtttgt
    11881 ccacatcact attatcagga tttaaatctc cctcaatcaa aaaatgacca cgaaacttaa
    11941 tcatatgcgc taaggccaaa tagattaagc gcaaatccgc tttatcagta gaatctacca
    12001 atttttttcg cagatgatag atagttggat atttctcatg ataagcaact tcatctacta
    12061 tatttccaaa aataggatga cgttcatgct tcttgtcttc ttccaccaaa aaagactctt
    12121 caagtcgatg aaagaaacta tcatctactt tcgccatctc atttgaaaaa atctcctgta
    12181 gataacaaat acgattcttc cgacgtgtat accttctacg agctgtccgt ttgagacgag
    12241 tcgcttccgc tgtctctcca ctgtcaaata aaagagcccc tataagattt tttttgatac
    12301 tgtggcggtc tgtatttccc agaaccttga actttttaga cggaacctta tattcatcag
    12361 tgatcaccgc ccatccgacg ctatttgtgc cgatagctaa gcctattgag tatttcttat
    12421 ccatggtgag tgcctcctta taatttattt tgtagttcct tcgaacgaaa tcattgtatc
    12481 taacaaactt cagaatttaa tcagagccgt ttattatgct cgcgttatcg acaataatat
    12541 tattaccaat actttctcaa gatagaatta agactgtttt agatttgtta atgtttctat
    12601 tgtcagtata gttataagac t
//