| 质粒名称: | LRG-2.1T-neo-sgROSA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 105587 |
| 存储实验室: | Christopher Vakoc |
|
载体骨架基本信息 |
|
| 载体名称: | LRG 2.1T(hU6-sgRNA- EFS-GFP-P2A-Neo) |
| 载体抗性: | 氨苄青霉素 |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 新霉素（G418） |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | gRNA targeting rosa26 locus |
| 物种来源: | M. musculus (mouse) |
| 启动子 | hU6 |
| 克隆方法: | Ligation Independent克隆 |
| 5' 测序引物 | AACCGGTGCCTAGAGAAGGT |
|
文献引用方法 |
|
| 材料和方法部分: | LRG-2.1T-neo-sgROSA was a gift from Christopher Vakoc (Addgene plasmid # 105587 ; http://n2t.net/addgene:105587 ; RRID:Addgene_105587) |
| 参考文献部分: | A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia. Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander EL, Wilkinson JE, Challen GA, Vakoc CR. Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi: 10.1016/j.ccell.2017.12.002. 10.1016/j.ccell.2017.12.002 PubMed 29316427 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS LRG-2.1T-neo-sgROSA 8307 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION lentivirally express gRNA targeting ROSA26 locus with GFP marker and
neo resistance.
ACCESSION .
VERSION .
KEYWORDS LRG-2.1T-neo-sgROSA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8307)
AUTHORS Xu Y, Milazzo JP, Somerville TDD, Tarumoto Y, Huang YH, Ostrander
EL, Wilkinson JE, Challen GA, Vakoc CR
TITLE A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute
Myeloid Leukemia.
JOURNAL Cancer Cell. 2018 Jan 8;33(1):13-28.e8. doi:
10.1016/j.ccell.2017.12.002.
PUBMED 29316427
REFERENCE 2 (bases 1 to 8307)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8307
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 5..231
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 232..412
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 459..584
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1077..1310
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1495..1539
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
promoter 1717..1957
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 1717..1737
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 1888..1907
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_feature 2130..2247
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2316..2527
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 2547..2556
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 2556..2579
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 2580..3296
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
CDS 2580..3296
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2625..2646)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2886..2905)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 3233..3254
/label=EGFP-C
/note="EGFP, forward primer"
CDS 3306..3362
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 3363..4157
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MLEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3417..3436)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4027..4046
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
misc_feature 4164..4752
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4217..4237)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4635..4646)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4824..5057
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5135..5256
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5172..5191)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5226..5245
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5296..5431
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 5358..5377
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(5451..5470)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5452..5470)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5480..5497)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5480..5496)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5489..5511)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 5638..6093
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5725..5744)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5935..5956
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6119..6223
/gene="bla"
/label=AmpR promoter
CDS 6224..7084
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6442..6461)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7255..7843
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7744..7763
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 7997..8014
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8131..8152
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8167..8197
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8205..8221
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8210..8232
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8229..8245
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8229..8245
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 8264..8284
/label=T3
/note="T3 promoter, forward primer"
promoter 8266..8284
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
1681 tttcagaccc acctcccaac cccgagggga cccagagagg gcctatttcc catgattcct
1741 tcatatttgc atatacgata caaggctgtt agagagataa ttagaattaa tttgactgta
1801 aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt
1861 gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat
1921 ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgaaga tgggcgggag
1981 tcttcgttta agagctatgc tggaaacagc atagcaagtt taaataaggc tagtccgtta
2041 tcaactttgc tggaaacagc aaagtggcac cgagtcggtg ctttttttga attctagatc
2101 ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
2161 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
2221 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt
2281 tggcgccggc tcgagtggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt
2341 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg
2401 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga
2461 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag
2521 aacacaggtg tcgtgacgcg ggatccgcca ccatggatta caaagacgat gacgataaga
2581 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
2641 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
2701 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
2761 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
2821 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
2881 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
2941 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
3001 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
3061 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
3121 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
3181 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
3241 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagggca
3301 gcggcgccac caacttcagc ctgctgaagc aggccggcga cgtggaggag aaccccggcc
3361 ccatgcttga acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat
3421 tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt
3481 cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac
3541 tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg
3601 tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc
3661 aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa
3721 tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc
3781 gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg
3841 aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg
3901 acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa
3961 atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg
4021 acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct
4081 tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc
4141 ttgacgagtt cttctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
4201 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
4261 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
4321 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
4381 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
4441 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
4501 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
4561 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
4621 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
4681 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
4741 ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca
4801 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
4861 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
4921 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
4981 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
5041 gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata
5101 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg
5161 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
5221 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg
5281 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
5341 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
5401 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg
5461 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
5521 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
5581 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg
5641 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
5701 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
5761 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
5821 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
5881 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
5941 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
6001 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
6061 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg
6121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
6181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
6241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
6301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
6361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
6421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
6481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
6541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
6601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
6661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
6721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
6781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
6841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
6901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
6961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
7021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
7081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
7141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
7201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
7261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
7321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
7381 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
7441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
7501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
7561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
7621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
7681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
7741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
7801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
7861 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
7921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
7981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
8041 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
8101 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
8161 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
8221 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
8281 aagggaacaa aagctggagc tgcaagc
//
