| 质粒名称: | pORI28 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 71595 |
| 存储实验室: | Todd Klaenhammer, Jan Kok |
|
载体骨架基本信息 |
|
| 载体名称: | pWV01 |
| 载体出品公司: | -- |
| 空载体大小: | 2200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | RepA- |
| 载体类型: | 细菌整合载体 |
| 筛选标记: | -- |
| 载体抗性: | 红霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | E. coli EC1000 provides RepA in trans |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pORI28 was a gift from Todd Klaenhammer & Jan Kok (Addgene plasmid # 71595 ; http://n2t.net/addgene:71595 ; RRID:Addgene_71595) |
| 参考文献部分: | A general system for generating unlabelled gene replacements in bacterial chromosomes. Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I, Dabrowska M, Venema G, Kok J. Mol Gen Genet. 1996 Nov 27;253(1-2):217-24. PubMed 9003306 |
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载体基本信息
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载体简介
载体序列
LOCUS pORI28 1690 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION EC1000 containing pORI28. Used as the source of plasmid pORI28. With
pTRK669, part of a system for chromosomal integration in gram
positive bacteria..
ACCESSION .
VERSION .
KEYWORDS pORI28
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 1690)
AUTHORS Leenhouts K, Buist G, Bolhuis A, ten Berge A, Kiel J, Mierau I,
Dabrowska M, Venema G, Kok J
TITLE A general system for generating unlabelled gene replacements in
bacterial chromosomes.
JOURNAL Mol Gen Genet. 1996 Nov 27;253(1-2):217-24.
PUBMED 9003306
REFERENCE 2 (bases 1 to 1690)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..1690
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 193..204
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 actcggcact taatgggggg tcgtagtacg gaagcaaaat tcgcttcctt tccccccatt
61 tttttccaaa ttccaaattt ttttcaaaaa ttttccagcg ctaccgctcg gcaaaattgc
121 aagcaatttt taaaatcaaa cccatgaggg aatttcattc cctcatactc ccttgagcct
181 cctccaaccg aaatagaagg gcgctgcgct tattatttca ttcagtcatc ggctttcata
241 atctaacaga caacatcttc gctgcaaagc cacgctacgc tcaagggctt ttacgctacg
301 ataacgcctg ttttaacgat tatgccgata actaaacgaa ataaacgcta aaacgtctca
361 gaaacgattt tgagacgttt taataaaaaa tcgctagtcc gaggcctcga cccgattcac
421 aaaaaatagg cacacgaaaa acaagttaag ggatgcagtt tatgcatccc ttaacttact
481 tattaaataa tttatagcta ttgaaaagag ataagaattg ttcaaagcta atattgttta
541 aatcgtcaat tcctgcatgt tttaaggaat tgttaaattg attttttgta aatattttct
601 tgtattcttt gttaacccat ttcataacga aataattata cttttgttta tctttgtgtg
661 atattcttga tttttttcta cttaatctga taagtgagct attcacttta ggtttaggat
721 gaaaatattc tcttggaacc atacttaata tagaaatatc aacttctgcc attaaaagta
781 atgccaatga gcgttttgta tttaataatc ttttagcaaa cccgtattcc acgattaaat
841 aaatctcatt agctatacta tcaaaaacaa ttttgcgtat tatatccgta cttatgttat
901 aaggtatatt accatatatt ttataggatt ggtttttagg aaatttaaac tgcaatatat
961 ccttgtttaa aacttggaaa ttatcgtgat caacaagttt attttctgta gttttgcata
1021 atttatggtc tatttcaatg gcagttacga aattacacct ctttactaat tcaagggtaa
1081 aatggccttt tcctgagccg atttcaaaga tattatcatg ttcatttaat cttatatttg
1141 tcattatttt atctatatta tgttttgaag taataaagtt ttgactgtgt tttatatttt
1201 tctcgttcat tataaccctc tttaatttgg ttatatgaat tttgcttatt aacgattcat
1261 tataaccact tattttttgt ttggttgata atgaactgtg ctgattacaa aaatactaaa
1321 aatgcccata ttttttcctc cttataaaat tagtataatt atagcacggt cgagatctat
1381 cgatgcatgc catggtaccc gggagctcga attcgaagct tctgcagacg cgtcgacgtc
1441 atatggatcc gatatcgccg tggcggccgc tctagaacta gcgattctga aatcaccatt
1501 taaaaaactc caatcaaata attttataaa gttagtgtat cactttgtaa tcataaaaac
1561 aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg ttggcgattc
1621 gttggcgatt gaaaaacccc ttaaaccctt gagccagttg ggatagagcg tttttggcac
1681 aaaaattggc
//
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