PCDH-CMV-mCherry-TRIM21

LOCUS       PCDH-CMV-mCherry-TRIM21              8580 bp DNA     circular SYN 30-JUL-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8580)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 7, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             234..414
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    458..583
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1076..1309
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1798..1915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1904..1953
                     /label=Primer 4
     enhancer        1938..2241
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2242..2445
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(2444..2491)
                     /label=Primer 1
     primer_bind     2466..2500
                     /label=Primer 2
     primer_bind     2466..2492
                     /label=Primer 5
     CDS             2496..3203
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3025..3046
                     /label=mcherry-F
     misc_feature    3204..3232
                     /label=MCS
                     /note="multiple cloning site"
     primer_bind     3218..3257
                     /label=mcherry-TRIM21-F
     misc_feature    3240..4667
                     /label=TRIM21
     primer_bind     complement(4647..4692)
                     /label=mcherry-TRIM21-R
     misc_feature    4677..5264
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(5148..5159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5337..5570
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5642..5763
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5782..5917
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5955..5971)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5979..5995
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6003..6033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6048..6069
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6357..6945)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7116..7976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7977..8081)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8450..8463)
                     /label=Primer 5
     primer_bind     8555..8571
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
     1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
     1921 tactagttgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
     1981 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
     2041 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
     2101 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
     2161 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
     2221 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
     2281 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
     2341 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
     2401 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt
     2461 cagattctag accgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
     2521 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     2581 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     2641 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     2701 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     2761 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     2821 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     2881 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     2941 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     3001 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     3061 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     3121 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     3181 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcggaattca
     3241 tggcttcagc agcacgcttg acaatgatgt gggaggaggt cacatgccct atctgcctgg
     3301 accccttcgt ggagcctgtg agcatcgagt gtggccacag cttctgccag gaatgcatct
     3361 ctcaggttgg gaaaggtggg ggcagcgtct gtcctgtgtg ccggcagcgc tttctgctca
     3421 agaatctccg gcccaatcga cagctagcca acatggtgaa caaccttaaa gaaatcagcc
     3481 aggaggccag agagggcaca cagggggaac ggtgtgcagt gcatggagag agacttcacc
     3541 tgttctgtga gaaagatggg aaggcccttt gctgggtatg tgcccagtct cggaaacacc
     3601 gtgaccacgc catggtccct cttgaggagg ctgcacagga gtaccaggag aagctccagg
     3661 tggcattagg ggaactgaga agaaagcagg agttggctga gaagttggaa gtggaaattg
     3721 caataaagag agcagactgg aagaaaacag tggaaacaca gaaatctagg attcacgcag
     3781 agtttgtgca gcaaaaaaac ttcctggttg aagaagaaca gaggcagctg caggagctgg
     3841 agaaggatga gagggagcag ctgagaatcc tgggggagaa agaggccaag ctggcccagc
     3901 agagccaggc cctacaggag ctcatctcag agctagatcg aaggtgccac agctcagcac
     3961 tggaactgct gcaggaggtg ataattgtcc tggaaaggag tgagtcctgg aacctgaagg
     4021 acctggatat tacctctcca gaactcagga gtgtgtgcca tgtgccaggg ctgaagaaga
     4081 tgctgaggac atgtgcagtc cacatcactc tggatccaga cacagccaat ccgtggctga
     4141 tactttcaga agatcggaga caagtgaggc ttggagacac ccagcagagc atacctggaa
     4201 atgaagagag atttgatagt tatcctatgg tcctgggtgc ccagcacttt cactctggaa
     4261 aacattactg ggaggtagat gtgacaggaa aggaggcctg ggacctgggt gtctgcagag
     4321 actctgtgcg caggaagggg cactttttgc ttagttccaa gagtggcttc tggacaattt
     4381 ggttgtggaa caaacaaaaa tatgaggctg gcacctaccc ccagactccc ctccaccttc
     4441 aggtgcctcc atgccaagtt gggattttcc tggactatga ggctggcatg gtctccttct
     4501 acaacatcac tgaccatggc tccctcatct actccttctc tgaatgtgcc tttacaggac
     4561 ctctgcggcc cttcttcagt cctggtttca atgatggagg aaaaaacaca gcccctctaa
     4621 ccctctgtcc actgaatatt ggatcacaag gatccactga ctattgatag ggatccaatc
     4681 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4741 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4801 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4861 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4921 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4981 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5041 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5101 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5161 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc
     5221 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgccggtac ctttaagacc
     5281 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     5341 agggctaatt cactcccaac gaaaataaga tctgcttttt gcttgtactg ggtctctctg
     5401 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     5461 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     5521 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     5581 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     5641 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5701 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5761 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccagttccgc ccattctccg
     5821 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5881 ctattccaga agtagtgagg aggctttttt ggaggcctag acttttgcag agacggccca
     5941 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6001 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6061 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6121 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6181 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6241 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6301 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6361 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6421 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6481 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6541 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6601 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6661 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6721 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6781 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6841 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6901 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6961 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7021 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7081 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7141 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7201 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7261 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7321 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7381 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7441 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7501 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7561 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7621 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7681 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7741 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7801 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7861 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7921 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7981 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8041 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8101 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8161 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8221 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8281 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8341 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8401 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     8461 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     8521 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
//