pLOX-CW-CRE

价格:1000元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pLOX-CW-CRE
质粒类型: 慢病毒系统载体
高拷贝/低拷贝: 高拷贝
启动子: CMV
克隆方法: 多克隆位点,限制性内切酶
载体大小: 9886bp
5' 测序引物及序列: CMV forward : CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: SP6 : ATTTAGGTGACACTATAG
载体标签: --
载体抗性: 氨苄青霉素(Ampicillian)
筛选标记: --
备注: 克隆菌株为 Stbl3或HB101。
产品目录号: 12238
稳定性: --
组成型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLOX-CW-CRE载体图谱



载体简介

载体序列

LOCUS       pLOX-CW-CRE	9886 bp 	DNA	SYN
DEFINITION  pLOX-CW-CRE
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="pLOX-CW-CRE"
                     /mol_type="other DNA"
     misc_feature    457..637
                     /label="HIV-1_5_LTR"
     misc_feature    457..637
                     /label="truncHIV-1_3_LTR"
     misc_feature    748..792
                     /label="HIV-1_psi_pack"
     CDS             1180..2028
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    1302..1535
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     promoter        2040..2544
                     /label="CMV_immearly_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2047..2334
                     /label="CAG_enhancer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2501..2521
                     /label="CMV_fwd_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     promoter        2502..2571
                     /label="CMV_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2545..2564
                     /label="pCEP_fwd_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2547..2571
                     /label="LNCX_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     CDS             2640..3692
                     /label="ORF frame 3"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2643..2663
                     /label="SV40 NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2649..3692
                     /label="CRE_NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2649..2663
                     /label="NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     CDS             3682..4368
                     /label="ORF frame 1"
                     /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP
                     LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL
                     LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL
                     RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK
                     TNDLQGSCRS*"
     misc_feature    3720..4312
                     /label="WPRE"
                     /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP
                     LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL
                     LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL
                     RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK
                     TNDLQGSCRS*"
     CDS             complement(3805..4308)
                     /label="ORF frame 2"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4378..4399
                     /label="U3PPT"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4378..4393
                     /label="cPPT"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4450..4483
                     /label="loxP"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4503..4683
                     /label="HIV-1_5_LTR"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4503..4683
                     /label="truncHIV-1_3_LTR"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    complement(4709..4726)
                     /label="Sp6_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     gene            4964..5227
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    complement(5016..5035)
                     /label="pBRrevBam_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    5368..5390
                     /label="pGEX_3_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     promoter        5549..5577
                     /label="AmpR_promoter"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     gene            5619..6479
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     CDS             5619..6479
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6634..7253
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7479..7499)
                     /label="pBABE_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7485..7938)
                     /label="SV40_enhancer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        7497..7765
                     /label="SV40_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      7664..7741
                     /label="SV40_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    7726..7745
                     /label="SV40pro_F_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    8946..8961
                     /label="SV40_int"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    8967..9014
                     /label="SV40_3_splice"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             9091..9564
                     /label="ORF frame 1"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     misc_feature    9142..9162
                     /label="SV40 NLS"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     misc_feature    9148..9162
                     /label="NLS"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     terminator      9590..9721
                     /label="SV40_PA_terminator"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
     misc_feature    9678..9697
                     /label="EBV_rev_primer"
                     /translation="MPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFS
                     NRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINN
                     YAQKLCTFSFLICKGVNKEYLMYSALTRDHNQPYHICRGFTCFKKPPTPPPEPET*"
ORIGIN
    1 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
   61 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
  121 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
  181 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
  241 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
  301 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
  361 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
  421 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
  481 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
  541 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
  601 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
  661 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
  721 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
  781 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
  841 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
  901 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
  961 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
 1021 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
 1081 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
 1141 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
 1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
 1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
 1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
 1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
 1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
 1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
 1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
 1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
 1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
 1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
 1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
 1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
 1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
 1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgataagc ttgggagttc
 2041 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
 2101 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
 2161 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
 2221 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
 2281 tacatgacct tacgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
 2341 accatggtga tgcggttttg gcagtacacc aatgggcgtg gatagcggtt tgactcacgg
 2401 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
 2461 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
 2521 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
 2581 cgccatccac gctgttttga cctccataga agacaccgac tctagctaga ggatctacca
 2641 tgcccaagaa gaagaggaag gtgtccaatt tactgaccgt acaccaaaat ttgcctgcat
 2701 taccggtcga tgcaacgagt gatgaggttc gcaagaacct gatggacatg ttcagggatc
 2761 gccaggcgtt ttctgagcat acctggaaaa tgcttctgtc cgtttgccgg tcgtgggcgg
 2821 catggtgcaa gttgaataac cggaaatggt ttcccgcaga acctgaagat gttcgcgatt
 2881 atcttctata tcttcaggcg cgcggtctgg cagtaaaaac tatccagcaa catttgggcc
 2941 agctaaacat gcttcatcgt cggtccgggc tgccacgacc aagtgacagc aatgctgttt
 3001 cactggttat gcggcggatc cgaaaagaaa acgttgatgc cggtgaacgt gcaaaacagg
 3061 ctctagcgtt cgaacgcact gatttcgacc aggttcgttc actcatggaa aatagcgatc
 3121 gctgccagga tatacgtaat ctggcatttc tggggattgc ttataacacc ctgttacgta
 3181 tagccgaaat tgccaggatc agggttaaag atatctcacg tactgacggt gggagaatgt
 3241 taatccatat tggcagaacg aaaacgctgg ttagcaccgc aggtgtagag aaggcactta
 3301 gcctgggggt aactaaactg gtcgagcgat ggatttccgt ctctggtgta gctgatgatc
 3361 cgaataacta cctgttttgc cgggtcagaa aaaatggtgt tgccgcgcca tctgccacca
 3421 gccagctatc aactcgcgcc ctggaaggga tttttgaagc aactcatcga ttgatttacg
 3481 gcgctaagga tgactctggt cagagatacc tggcctggtc tggacacagt gcccgtgtcg
 3541 gagccgcgcg agatatggcc cgcgctggag tttcaatacc ggagatcatg caagctggtg
 3601 gctggaccaa tgtaaatatt gtcatgaact atatccgtaa cctggatagt gaaacagggg
 3661 caatggtgcg cctgctggaa gatggcgatt aggtcgagct caagcttcga attcgataat
 3721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
 3781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
 3841 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
 3901 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
 3961 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
 4021 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
 4081 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
 4141 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
 4201 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
 4261 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ggaattcgag
 4321 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
 4381 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc
 4441 accatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta
 4501 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
 4561 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
 4621 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
 4681 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
 4741 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
 4801 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
 4861 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
 4921 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
 4981 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
 5041 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
 5101 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
 5161 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
 5221 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
 5281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
 5341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
 5401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
 5461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
 5521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
 5581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
 5641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
 5701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
 5761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
 5821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
 5881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
 5941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
 6001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
 6061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
 6121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
 6181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
 6241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
 6301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
 6361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
 6421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
 6481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
 6541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
 6601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
 6661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
 6721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
 6781 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
 6841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
 6901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
 6961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
 7021 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
 7081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
 7141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
 7201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
 7261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
 7321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
 7381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
 7441 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
 7501 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
 7561 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
 7621 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
 7681 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
 7741 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
 7801 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
 7861 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
 7921 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
 7981 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
 8041 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
 8101 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
 8161 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
 8221 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
 8281 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
 8341 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
 8401 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
 8461 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
 8521 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
 8581 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
 8641 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
 8701 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
 8761 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
 8821 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
 8881 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
 8941 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
 9001 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
 9061 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
 9121 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
 9181 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
 9241 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
 9301 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
 9361 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
 9421 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
 9481 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
 9541 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
 9601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
 9661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
 9721 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
 9781 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
 9841 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagt
//

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