pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX

LOCUS      pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX          8386 bp DNA     circular SYN 23-JAN-2024
DEFINITION  Expresses the genetically-encoded fluorescent dopamine (DA) sensor 
            GRAB_rDA2m and a membrane-localized EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pDisplay-GRAB-rDA2m-IRES-EGFP-CAAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8386)
  TITLE     Improved dual-color GRAB sensors for monitoring dopaminergic 
            activity in vivo
REFERENCE   2  (bases 1 to 8386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 9, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8386
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        12..391
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        392..595
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     546..566
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     640..659
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        640..658
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             739..2574
                     /codon_start=1
                     /label=rDA2m
                     /translation="METDTLLLWVLLLWVPGSTGDTSLYKKVGTTGINRLNYSVLVNLT
                     AYYDGGAGLNLSSVNCTSSIVAGGAVRPGECAGTAGVDEKSNANSWWALILVIVPCLTL
                     FGNVLVILAVVKERTLQTVTNYFIVSLAVADLLVAVLVMPFAVYVLVNGSWSLPGFVCD
                     FYIAMDVTCSTSSIFNLVAISIDRYIAVTQPIKYAKHKNNRRVWLTILLVWAISAAIGS
                     PIVLGLNNTPDRIPDACLFYNTDFIIYSSLSSFYIPCIIMVFLYYNIFKALRNRAKLFR
                     ASCVCSEVMYPEDGALKSEIWKGLRLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLD
                     IVSHNEDYTIVEQCERAEARHSTGGMDELYKGGTGGSLVSKGEEDNMAIIKEFMRFKVH
                     MEGSVNGHEFEIEGEGEGRPYEAFQTAKLKVTKGGPLPFTWDILSPQFMYGSKAYIKHP
                     ADIPDYFKLSFPEGFRWERVMNFEDGGIIHVNQDSSLQDGYFIYKVKLRGTNFPPDGPV
                     MQKKTMGWELTVLERKATKPLAIVLGVFLICWVPFFTCNIMDAICTKLTKACQPGVTAF
                     IVTSWLGYMNSFVNPVIYTVFNPEFRKAFRKLISI"
     sig_peptide     739..801
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTGD"
     protein_bind    802..826
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     2283..2302
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    2601..3152
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2745..2762)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2971..2990
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3164..3880
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3164..3880
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3209..3230)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3470..3489)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3817..3838
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3938..3967
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             3971..4117
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     complement(4139..4156)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4145..4369
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4501..5089)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4581..4600)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5418..5465
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5490..5509
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5697..6491)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5808..5827)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6418..6437
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     complement(6590..6609)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     enhancer        6688..6879
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6836..6856
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(6939..7799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7562..7581
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7800..7904)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7931..8386
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8018..8037)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8228..8249
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acgcgcgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
       61 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
      121 ctgaccgccc aacgacccct gcccattgac gtcaataatg acgtatgttc ccatagtaac
      181 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
      241 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
      301 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
      361 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
      421 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
      481 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
      541 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctctctg
      601 gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
      661 gacccaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt gctggaattc
      721 ggcttgggga tatccaccat ggagacagac acactcctgc tatgggtact gctgctctgg
      781 gttccaggtt ccactggtga cacaagtttg tacaaaaaag ttggcaccac cggtatcaac
      841 cgactgaact acagcgtcct cgtgaacctg acggcctact acgacggcgg cgcgggtctg
      901 aatctcagca gcgtcaattg cacgtcgtcg atagtcgccg ggggtgccgt tagaccgggc
      961 gagtgcgctg gaaccgccgg cgtcgacgag aagtctaacg cgaacagctg gtgggccctg
     1021 atacttgtga tcgtgccgtg tttgacgctc ttcggtaatg tgctcgtcat cctagcggtg
     1081 gtgaaagaac gaacgctgca gactgtcact aactacttca tcgtcagttt ggcggtcgcc
     1141 gatcttctcg tggcggtgct cgttatgccg ttcgcggtct acgtgctggt gaacggatcc
     1201 tggagtttac ccgggtttgt ttgtgatttt tatatcgcaa tggatgtgac ttgcagcacc
     1261 agctctatat tcaatctcgt ggctatttca atagacagat acatagcggt gacccagccg
     1321 ataaagtacg cgaagcacaa gaacaataga agagtatggt tgacgatact gttggtctgg
     1381 gcgatatcgg ccgcgatcgg cagcccgatt gtcctaggct tgaataacac tccggaccgg
     1441 atcccggacg catgtctgtt ctacaatacg gatttcatca tctactcgag cctgtccagc
     1501 ttctacatac cctgcatcat catggtattc ctttactaca atatattcaa ggctctacga
     1561 aacagagcga aactttttcg tgctagctgc gtgtgttccg aggttatgta ccccgaggac
     1621 ggcgccctga agagcgagat ctggaagggg ctgaggctga aggacggcgg ccactacgcc
     1681 gccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc
     1741 gtcgacatca agttggacat cgtgtcccac aacgaggact acaccatcgt ggaacagtgc
     1801 gaacgcgccg aggcccgcca ctccaccggc ggcatggacg agctgtacaa gggaggtaca
     1861 ggcgggagtc tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1921 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1981 ggcgagggcc gcccctacga ggcctttcag accgctaagc tgaaggtgac caagggtggc
     2041 cccctgccct tcacttggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     2101 attaagcacc cagccgacat ccccgactac ttcaagctgt ccttccccga gggcttcagg
     2161 tgggagcgcg tgatgaactt cgaggacggc ggcattattc acgttaacca ggactcctcc
     2221 ctgcaggacg gctacttcat ctacaaggtg aagctgcgcg gcaccaactt cccccccgac
     2281 ggccccgtaa tgcagaagaa gaccatgggc tgggagctga cggtcctcga gcgcaaggcc
     2341 acgaagcctc tggcgatcgt gttaggtgtc tttctaatat gctgggtgcc cttcttcact
     2401 tgtaacatca tggatgcaat ctgcacgaag ctgacgaagg cttgtcagcc tggcgttaca
     2461 gccttcatcg tcacttcctg gttgggctac atgaacagct tcgtaaatcc cgtaatatac
     2521 actgtgttca accccgaatt ccgtaaggcc ttccgcaagc tgatcagcat ataagctagc
     2581 gcccctctcc ctccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc
     2641 gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa
     2701 acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat
     2761 gcaaggtctg ttaaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac
     2821 aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg
     2881 cggccaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     2941 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     3001 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtacaca
     3061 tgctttacat gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac
     3121 gtggttttcc tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga
     3181 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3241 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3301 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3361 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3421 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3481 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     3541 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     3601 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     3661 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     3721 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     3781 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     3841 cgccgccggg atcactctcg gcatggacga gctgtacaag gatggtaaaa agaagaaaaa
     3901 gaagtcaaag acaaagtgtg taattatgta agtcgacgaa caaaaactca tctcagaaga
     3961 ggatctgaat gctgtgggcc aggacacgca ggaggtcatc gtggtgccac actccttgcc
     4021 ctttaaggtg gtggtgatct cagccatcct ggccctggtg gtgctcacca tcatctccct
     4081 tatcatcctc atcatgcttt ggcagaagaa gccacgttag gcggccgctc gagatcagcc
     4141 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     4201 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     4261 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     4321 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     4381 gaaagaacca gtggcggtaa tacggttatc cacagaatcg ggggataacg caggaaagaa
     4441 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     4501 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     4561 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     4621 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     4681 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4741 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4801 ctatcgtctt gagtccaacc cggtaagacg cgacttatcg ccactggcag cagccactgg
     4861 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4921 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4981 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     5041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5221 atcaatctaa agtatatatg agtaacctga ggctatggca gggcctgccg ccccgacgtt
     5281 ggctgcgagc cctgggcctt cacccgaact tggggggtgg ggtggggaaa aggaagaaac
     5341 gcgggcgtat tggccccaat ggggtctcgg tggggtatcg acagagtgcc agccctggga
     5401 ccgaaccccg cgtttatgaa caaacgaccc aacaccgtgc gttttattct gtctttttat
     5461 tgccgtcata gcgcgggttc cttccggtat tgtctccttc cgtgtttcag ttagcctccc
     5521 cctagggtgg gcgaagaact ccagcatgag atccccgcgc tggaggatca tccagccggc
     5581 gtcccggaaa acgattccga agcccaacct ttcatagaag gcggcggtgg aatcgaaatc
     5641 tcgtgatggc aggttgggcg tcgcttggtc ggtcatttcg aaccccagag tcccgctcag
     5701 aagaactcgt caagaaggcg atagaaggcg atgcgctgcg aatcgggagc ggcgataccg
     5761 taaagcacga ggaagcggtc agcccattcg ccgccaagct cttcagcaat atcacgggta
     5821 gccaacgcta tgtcctgata gcggtccgcc acacccagcc ggccacagtc gatgaatcca
     5881 gaaaagcggc cattttccac catgatattc ggcaagcagg catcgccatg ggtcacgacg
     5941 agatcctcgc cgtcgggcat gctcgccttg agcctggcga acagttcggc tggcgcgagc
     6001 ccctgatgct cttcgtccag atcatcctga tcgacaagac cggcttccat ccgagtacgt
     6061 gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc aggtagccgg atcaagcgta
     6121 tgcagccgcc gcattgcatc agccatgatg gatactttct cggcaggagc aaggtgagat
     6181 gacaggagat cctgccccgg cacttcgccc aatagcagcc agtcccttcc cgcttcagtg
     6241 acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg ccagccacga tagccgcgct
     6301 gcctcgtctt gcagttcatt cagggcaccg gacaggtcgg tcttgacaaa aagaaccggg
     6361 cgcccctgcg ctgacagccg gaacacggcg gcatcagagc agccgattgt ctgttgtgcc
     6421 cagtcatagc cgaatagcct ctccacccaa gcggccggag aacctgcgtg caatccatct
     6481 tgttcaatca tgcgaaacga tcctcatcct gtctcttgat cgatctttgc aaaagcctag
     6541 gcctccaaaa aagcctcctc actacttctg gaatagctca gaggccgggc ctcggcctct
     6601 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     6661 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     6721 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     6781 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     6841 aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt tttcaataaa
     6901 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6961 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     7021 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     7081 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     7141 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     7201 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     7261 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     7321 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     7381 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     7441 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7501 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7561 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7621 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7681 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7741 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7801 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7861 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7921 gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     7981 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8041 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8101 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8161 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     8221 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     8281 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     8341 caaaaattta acgcgaattt taacaaaata ttaacgctta caattt
//