| 质粒名称: | p3TP-lux |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 11767 |
| 存储实验室: | Joan Massague, Jeff Wrana |
|
载体骨架基本信息 |
|
| 载体名称: | pGL2-Basic |
| 载体出品公司: | Promega |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,荧光素酶 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 3TP |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI (未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | LucNrev |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p3TP-lux was a gift from Joan Massague & Jeff Wrana (Addgene plasmid # 11767 ; http://n2t.net/addgene:11767 ; RRID:Addgene_11767) |
| 参考文献部分: | TGF beta signals through a heteromeric protein kinase receptor complex. Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang XF, Massague J. Cell. 1992 Dec 11. 71(6):1003-14. 10.1016/0092-8674(92)90395-S PubMed 1333888 |
买家导航
p3TPlux载体基本信息
p3TPlux载体图谱质粒图谱和多克隆位点信息
p3TPlux载体简介
p3TPlux载体序列
LOCUS p3TP-lux 5905 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p3TP-lux
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5905)
AUTHORS Wrana JL, Attisano L, Carcamo J, Zentella A, Doody J, Laiho M, Wang
XF, Massague J
TITLE TGF beta signals through a heteromeric protein kinase receptor
complex.
JOURNAL Cell. 1992 Dec 11. 71(6):1003-14.
PUBMED 1333888
REFERENCE 2 (bases 1 to 5905)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5905
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 383..2035
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
primer_bind complement(449..467)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
intron 2276..2341
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2471..2491
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2916..3050
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3192..3209)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3363..3951)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3443..3462)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4122..4982)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4745..4764
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4983..5087)
/gene="bla"
/label=AmpR promoter
rep_origin 5114..5569
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5201..5220)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5411..5432
/label=F1ori-F
/note="F1 origin, forward primer"
polyA_signal 5759..5880
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
ORIGIN
1 cccgggaggt accgagctct tacgcgtgct agctcgagca gctgaagctc ccttccagaa
61 agcagaggtg tctgactcat atgagtcaga cacctctggc tgtctggaag ggatgagtca
121 gacacctctg gctttctgga agggagcttg catgccctag aggatccaac ctcagccaga
181 caaggttgtt gacacaagag agccctcagg ggcacagaga gagtctggac acgtgggggt
241 cagccgtgta tcatcggagg atccccagtc ctatatatac tcgctctgca cttggccctt
301 ttttacactg tgactgattg agctggtgcc gtgtcggggt accgagctcg aattagcttg
361 gcattccggt actgttggta aaatggaaga cgccaaaaac ataaagaaag gcccggcgcc
421 attctatcct ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata
481 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga acatcacgta
541 cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
601 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
661 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
721 attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca aaaaggggtt
781 gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta ttatcatgga
841 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
901 tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca aaacaattgc
961 actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc ttccgcatag
1021 aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
1081 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
1141 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
1201 tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa ccctattttc
1261 attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
1321 ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac gcttccatct
1381 tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc tgattacacc
1441 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
1501 tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat tatgtgtcag
1561 aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
1621 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
1681 catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg cccccgctga
1741 attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg caggtcttcc
1801 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
1861 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
1921 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
1981 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat tgtaaaatgt
2041 aactgtattc agcgatgacg aaattcttag ctattgtaat actgcgatga gtggcagggc
2101 ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg ctacgcctga
2161 ataagtgata ataagcggat gaatggcaga aattcgccgg atctttgtga aggaacctta
2221 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
2281 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
2341 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
2401 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
2461 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
2521 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
2581 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
2641 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
2701 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
2761 ttgtaaaggg gttaataagg aatatttgat gtattgtgcc ttgactagag atcataatca
2821 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
2881 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
2941 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
3001 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgtcgaccga
3061 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg
3121 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc
3181 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
3241 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
3301 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
3361 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
3421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
3481 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
3541 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
3601 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
3661 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
3721 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
3781 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
3841 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
3901 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
3961 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
4021 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
4081 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
4141 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
4201 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
4261 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
4321 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
4381 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
4441 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
4501 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
4561 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
4621 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
4681 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
4741 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
4801 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
4861 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
4921 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
4981 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
5041 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
5101 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
5161 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
5221 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
5281 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
5341 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
5401 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
5461 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
5521 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca
5581 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
5641 cccaagctac catgataagt aagtaatatt aaggtacgtg gaggttttac ttgctttaaa
5701 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
5761 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
5821 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
5881 tggtactgta actgagctaa cataa
//
