| 质粒名称: | pAAV-CAG-miRFP680-P2A-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000009 |
| 产品用途: | AAV, Expresses miiRFP680 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
DH5a, TOP10
|
|
插入基因信息 |
|
| 插入基因名称: | miRFP680, EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pAAV-CAG-miRFP680-P2A-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-miRFP680-P2A-GFP载体质粒基本信息
pAAV-CAG-miRFP680-P2A-GFP质粒图谱载体图谱和pAAV-CAG-miRFP680-P2A-GFP载体序列质粒序列多克隆位点信息
pAAV-CAG-miRFP680-P2A-GFP质粒载体简介
pAAV-CAG-miRFP680-P2A-GFP质粒序列载体序列
LOCUS pAAV-CAG-miRFP680-P2A-GFP 6481 bp DNA circular SYN 26-OCT-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6481)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jun 22, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6481
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 983..1930
/codon_start=1
/label=miRFP680
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGL
IVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE"
primer_bind complement(1909..1953)
/label=0117-miRFP680-R
CDS 1955..2011
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 2024..2740
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2765..3353
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3236..3247)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3396..3517
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4045..4633)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4804..5664)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5665..5769)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5795..6250)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggcgga aggctccgtc gccaggcagc ctgacctctt
1021 gacctgcgac gatgagccga tccatatccc cggtgccatc caaccgcatg gactgctgct
1081 cgccctcgcc gccgacatga cgatcgttgc cggcagcgac aaccttcccg aactcaccgg
1141 actggcgatc ggcgccctga tcggccgctc tgcggccgat gtcttcgact cggagacgca
1201 caaccgtctg acgatcgcct tggccgagcc cggggcggcc gtcggagcac cgatcactgt
1261 cggcttcacg atgcgaaagg acgcaggctt catcggctcc tggcatcgcc atgatcagct
1321 catcttcctc gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg
1381 ccgcaccaac agcgccatcc gccgccttca ggccgccgaa accttggaaa gcgcctgcgc
1441 cgccgcggcg caagaggtgc ggaagattac cggcttcgat cgggtgatga tctatcgctt
1501 cgcctccgac ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa
1561 actaggcctg cactatcctg cctcaaccgt gccggcgcag gcccgtcggc tctataccat
1621 caacccggta cggatcattc ccgatatcaa ttatcggccg gtgccggtca ccccagacct
1681 caatccggtc accgggcggc cgattgatct tagcttcgcc atcctgcgca gcgtctcgcc
1741 ctgccatctg gagttcatgc gcaacatagg catgcacggc acgatgtcga tctcgatttt
1801 gcgcggcgag cgactgtggg gattgatcgt ttgccatcac cgaacgccgt actacgtcga
1861 tctcgatggc cgccaagcct gcaagagggt cgccgagagg ctggccactc agatcggcgt
1921 gatggaagag cgaccggttc acgtgggaag tggggctaca aacttctcac tgctgaaaca
1981 ggccggggat gtggaggaaa acccaggacc acacgtgact agtgtgagca agggcgagga
2041 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
2101 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
2161 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
2221 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
2281 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
2341 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
2401 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
2461 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
2521 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
2581 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
2641 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
2701 cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcgata tcaagcttat
2761 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
2821 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
2881 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
2941 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
3001 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
3061 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
3121 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
3181 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
3241 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
3301 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
3361 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
3421 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
3481 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
3541 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
3601 atgtggtaaa atcgataagg atcttcctag agcatggcta cgtagataag tagcatggcg
3661 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
3721 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
3781 cggcctcagt gagcgagcga gcgcgcagct gcattaatga atcggccaac gcgcggggag
3841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
3901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
3961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
4021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
4081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
4141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
4201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
4261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
4321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
4381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
4441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
4501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
4561 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
4621 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
4681 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
4741 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
4801 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
4861 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
4921 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
4981 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
5041 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
5101 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
5161 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
5221 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
5281 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
5341 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
5401 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
5461 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
5521 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
5581 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
5641 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
5701 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
5761 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
5821 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
5881 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
5941 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
6001 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
6061 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
6121 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
6181 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
6241 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
6301 gcctcttcgc tattacgcca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag
6361 cccgggcgtc gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag
6421 ggagtggcca actccatcac taggggttcc ttgtagttaa tgattaaccc gccatgctac
6481 t
//
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