pGL3-control

价格:1000元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Promega
载体名称: pGL3-control
质粒类型: 哺乳细胞表达;启动子增强子报告系统
高拷贝/低拷贝: --
启动子: SV40
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5256bp
5' 测序引物及序列: RVprimer3 Fwd: CTAGCAAAATAGGCTGTCCC
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素(Ampicillin)
筛选标记: --
备注: pGL3-control是荧光素酶报告载体,含有SV40 增强子和启动子,增强荧光素酶的表达。
产品目录号: E1741
稳定性: --
组成型: --
病毒/非病毒: 非病毒

载体质粒图谱和多克隆位点信息

pGL3-control载体图谱



pGL3-control多克隆位点

pGL3-control载体特征


载体简介

载体序列

LOCUS       pGL3-Control	5256 bp 	DNA	SYN
DEFINITION  pGL3-Control
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5256
                     /organism="pGL3-Control"
                     /mol_type="other DNA"
     promoter        48..250
                     /label="SV40_promoter"
     rep_origin      95..172
                     /label="SV40_origin"
     misc_feature    157..176
                     /label="SV40pro_F_primer"
     gene            280..1932
                     /label="firefly_Luciferase"
                     /gene="firefly_Luciferase"
     CDS             280..1932
                     /label="ORF frame 1"
     misc_feature    complement(346..364)
                     /label="LucNrev_primer"
     misc_feature    complement(1984..2003)
                     /label="EBV_rev_primer"
     misc_feature    2209..2436
                     /label="SV40_enhancer"
     misc_feature    2416..2436
                     /label="pBABE_3_primer"
     rep_origin      complement(2744..3363)
                     /label="pBR322_origin"
     gene            complement(3518..4378)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(3518..4378)
                     /label="ORF frame 3"
     promoter        complement(4420..4448)
                     /label="AmpR_promoter"
     rep_origin      4527..4833
                     /label="f1_origin"
     misc_feature    5096..5249
                     /label="TB"
     terminator      5096..5144
                     /label="synth_PA_terminator"
     terminator      5096..5249
                     /label="up_PA_terminator"
     misc_feature    5198..5217
                     /label="RVprimer3_primer"
ORIGIN
    1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctgc atctcaatta
   61 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
  121 cgcccattct ccgccccatc gctgactaat tttttttatt tatgcagagg ccgaggccgc
  181 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
  241 caaaaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
  301 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
  361 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
  421 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
  481 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
  541 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
  601 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
  661 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
  721 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
  781 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
  841 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
  901 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
  961 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
 1021 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
 1081 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
 1141 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
 1201 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
 1261 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
 1321 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
 1381 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
 1441 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
 1501 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
 1561 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
 1621 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
 1681 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
 1741 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
 1801 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
 1861 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
 1921 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
 1981 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
 2041 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
 2101 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
 2161 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc tgaacgatgg agcggagaat
 2221 gggcggaact gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg
 2281 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
 2341 cacacctggt tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc
 2401 ctggggactt tccacaccct aactgacaca cattccacag cggatccgtc gaccgatgcc
 2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc
 2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt
 2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
 2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
 2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
 2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
 2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
 2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
 2941 tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
 3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
 3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
 3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
 3181 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
 3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
 3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
 3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
 3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
 3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
 3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
 3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
 3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
 3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
 3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
 3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
 3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
 3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
 4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
 4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
 4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
 4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
 4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
 4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
 4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
 4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
 4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
 4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
 4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
 4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
 4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
 4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
 4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
 4921 aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat tcgccattca
 4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca
 5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa
 5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
 5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
 5221 tgcaagtgca ggtgccagaa catttctcta tcgata
//

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