pLVX-rHom-Nuc1

价格:4560元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-rHom-Nuc1
质粒类型: 慢病毒载体;双顺反子载体;信号通路载体;亚细胞定位载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子: CMV
载体大小: 9160 bp
5' 测序引物及序列: CMV Forward: CGCAAATGGGCGGTAGGCGTG
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢毒载体pLVX-rHom-Nuc1载体表达DmrD融合蛋白;
利用信号受体的可逆二聚化作用研究细胞信号通路;
DmrDN端带有核定位信号序列。
产品目录号: 635077
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-rHom-Nuc1载体图谱



pLVX-rHom-Nuc1 多克隆位点

pLVX-rHom-Nuc1 载体特征

载体简介

载体序列

LOCUS       pLVX-rHom-Nuc1   9160 bp    DNA   circular            10-NOV-2011
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Catalog Nos. 635077, 635076
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    2028..2151
                     /label=CTS/cppt\(DNA\flap)
     promoter        2185..2788
                     /label=pCMV
     misc_feature    5082..5673
                     /label=WPRE
     rep_origin      complement(6983..7653)
                     /label=pUC\origin
     CDS             complement(7798..8794)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     LTR             5877..6513
                     /label=3'\LTR
     misc_feature    3861..4436
                     /label=IRES
     CDS             4469..5068
                     /label=Puro(R)
     misc_feature    2822..2845
                     /label=NLS
     CDS             2849..3172
                     /label=DmrD
     CDS             3176..3499
                     /label=DmrD
     CDS             3503..3826
                     /label=DmrD
BASE COUNT     2353 a      2281 c      2388 g      2138 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc 
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 
     2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 
     2761 tccatagaag acaccgactc tagctagagg atctatttcc ggtgaattcc tcgagactag 
     2821 tatgcctaag aagaagagaa aggtgtctag aggagtgcag gtggagacta tctccccagg 
     2881 agacgggcgc accttcccca agcgcggcca gacctgcgtg gtgcactaca ccgggatgct 
     2941 tgaagatgga aagaaaatgg attcctcccg ggacagaaac aagcccttta agtttatgct 
     3001 aggcaagcag gaggtgatcc gaggctggga agaaggggtt gcccagatga gtgtgggtca 
     3061 gagagccaaa ctgactatat ctccagatta tgcctatggt gccactgggc acccaggcat 
     3121 catcccacca catgccactc tcgtcttcga tgtggagctt ctaaaactgg aaactagagg 
     3181 agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc gcggccagac 
     3241 ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaaatggatt cctcccggga 
     3301 cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag gctgggaaga 
     3361 aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc cagattatgc 
     3421 ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg tcttcgatgt 
     3481 ggagcttcta aaactggaaa ctagaggagt gcaggtggag actatctccc caggagacgg 
     3541 gcgcaccttc cccaagcgcg gccagacctg cgtggtgcac tacaccggga tgcttgaaga 
     3601 tggaaagaaa atggattcct cccgggacag aaacaagccc tttaagttta tgctaggcaa 
     3661 gcaggaggtg atccgaggct gggaagaagg ggttgcccag atgagtgtgg gtcagagagc 
     3721 caaactgact atatctccag attatgccta tggtgccact gggcacccag gcatcatccc 
     3781 accacatgcc actctcgtct tcgatgtgga gcttctaaaa ctggaaacta gttatagcgg 
     3841 ccgcggatcc tacgtatgac cgcccctctc cctccccccc ccctaacgtt actggccgaa 
     3901 gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc atattgccgt 
     3961 cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc attcctaggg 
     4021 gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag gaagcagttc 
     4081 ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg cagcggaacc 
     4141 ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat acacctgcaa 
     4201 aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga gtcaaatggc 
     4261 tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc cattgtatgg 
     4321 gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg ttaaaaaaac 
     4381 gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga tgataagctt 
     4441 gccacaaccc acaaggagac gaccttccat gaccgagtac aagcccacgg tgcgcctcgc 
     4501 cacccgcgac gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     4561 cgccacgcgc cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     4621 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     4681 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     4741 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     4801 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     4861 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     4921 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     4981 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 
     5041 gtgcatgacc cgcaagcccg gtgcctagac gcgtctggaa caatcaacct ctggattaca 
     5101 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     5161 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     5221 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     5281 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     5341 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     5401 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     5461 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     5521 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     5581 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     5641 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 
     5701 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 
     5761 agaagcacaa gaggaggagg aggtgggttt ttccagtcac acctcaggta cctttaagac 
     5821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag aggggactgg 
     5881 aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac 
     5941 aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga tatccactga 
     6001 cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata 
     6061 aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat gacccggaga 
     6121 gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc 
     6181 tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga ctttccgctg 
     6241 gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatcct 
     6301 gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc 
     6361 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 
     6421 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 
     6481 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 
     6541 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggccttgaca ttgctagcgt 
     6601 tttaccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 
     6661 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 
     6721 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 
     6781 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 
     6841 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     6901 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     6961 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     7021 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     7081 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     7141 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     7201 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     7261 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     7321 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     7381 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     7441 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 
     7501 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     7561 aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 
     7621 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 
     7681 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 
     7741 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 
     7801 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 
     7861 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 
     7921 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 
     7981 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 
     8041 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 
     8101 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 
     8161 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 
     8221 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 
     8281 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 
     8341 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 
     8401 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 
     8461 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 
     8521 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 
     8581 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 
     8641 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 
     8701 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 
     8761 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc aacttgttta 
     8821 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat 
     8881 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct 
     8941 ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta 
     9001 ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta 
     9061 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agtttttaaa 
     9121 gaaattgtat ttgttaaata tgtactacaa acttagtagt 
//

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