pLZ555

LOCUS       pLZ555          7408 bp DNA     circular SYN 14-JAN-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     234..250
                     /label=oMH18
     misc_feature    251..404
                     /label=Transcription blocker
                     /label=Transcription\blocker
     polyA_signal    251..299
                     /note="synthetic polyadenylation signal"
     misc_feature    313..404
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     misc_feature    438..524
                     /label=CRE reg_Regulatory_Seq
     misc_feature    449..456
                     /label=CRE
     misc_feature    485..492
                     /label=CRE*
     misc_feature    507..514
                     /label=CRE
     promoter        531..677
                     /label=P_TAL
     misc_recomb     693..726
                     /label=LoxP
     protein_bind    complement(693..726)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    776
                     /label=New Feature
     misc_feature    776
                     /label=New Feature
     misc_feature    785..1023
                     /label=GLP1-Fc
     CDS             926..1018
                     /codon_start=1
                     /label=GLP-1
                     /translation="HGEGTFTSDVSSYLEGQAAKEFIAWLVKGRG"
     misc_feature    929..931
                     /label=A-G
     misc_feature    971..973
                     /label=G-E
     misc_feature    1013..1015
                     /label=R-G
     CDS             1019..1732
                     /codon_start=1
                     /label=Fc
                     /translation="ISAMVRSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPE
                     VTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGK
                     EYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSD
                     IAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNH
                     YTQKSLSLSPGKASGS"
     primer_bind     1019..1042
                     /label=Hwtx-F
     CDS             1736..1792
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    1793..1861
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     CDS             1880..2395
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     misc_feature    1913..1915
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     2188..2217
                     /label=201-WKG-631-F2
     misc_feature    2402..2420
                     /label=SEAP
     polyA_signal    2452..2676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    2687..2808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     2906..2939
                     /label=LoxP
     protein_bind    complement(2906..2939)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    3005..3229
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3275..3703
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     primer_bind     complement(3289..3313)
                     /label=184-WKG-599-R
     primer_bind     3574..3593
                     /label=189-WKG-612-F
     promoter        3717..4046
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     misc_feature    3949..3998
                     /label=P_SV40 TSS-region
     CDS             4113..4907
                     /label=Translation 2136-2930
     misc_feature    4113..4904
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     polyA_signal    5081..5202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5247..5267)
                     /label=M13-rev
     primer_bind     complement(5251..5267)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_binding    complement(5273..5295)
                     /label=LacO
     protein_bind    5275..5291
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5299..5329)
                     /label=lac promoter
     protein_bind    5344..5365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    5471..6411
                     /label=ColE1
                     /note="ColE1"
     misc_feature    complement(6415..7272)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(7273..7377)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgtacttg
      241 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      301 atcgatagta ctaatatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
      361 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat aggtaccgag
      421 ctcttacgcg tgctagcgca ccagacagtg acgtcagctg ccagatccca tggccgtcat
      481 actgtgacgt ctttcagaca ccccattgac gtcaatggga gaacagatct gccgccccga
      541 ctgcatctgc gtgttcgaat tcgccaatga caagacgctg ggcggggttt gtgtcatcat
      601 agaactaaag acatgcaaat atatttcttc cggggacacc gccagcaaac gcgagcaacg
      661 ggccacgggg atgaagcaga aggctagcgc ttataacttc gtatagcata cattatacga
      721 agttatcggc gcgccgcggc cgcgatcaat tcggtaccga agttcctatt ccgaattcgc
      781 caccatgaag atcatcctgt ggctgtgtgt gttcggcctg ttcctggcca ccctgttccc
      841 catcagctgg cagatgcccg tggagtccgg cctgtcctcc gaggactccg ccagctccga
      901 gagcttcgcc aagcgcatca agcgccacgg cgagggcacc ttcaccagcg acgtgagcag
      961 ctacctggag ggccaggccg ccaaggagtt catcgcctgg ctggtgaagg gccgcggcat
     1021 atcggccatg gttagatctg acaaaactca cacatgccca ccgtgcccag cacctgaact
     1081 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
     1141 ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa
     1201 gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
     1261 gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct
     1321 gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa
     1381 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtacaccc tgcccccatc
     1441 ccgggaggag atgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
     1501 cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac
     1561 gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
     1621 gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcacgagg ctctgcacaa
     1681 ccactacacg cagaagagcc tctccctgtc tccgggtaaa gccagcggaa gtggagcaac
     1741 caacttttcc ctgctgaagc aggcaggcga cgtggaggag aatcctggac ccatgactag
     1801 tgagacagac acactcctgc tatgggtact gctgctctgg gttccaggtt ccactggtga
     1861 cgctagtggt ggttctggta tggtcttcac actcgaagat ttcgttgggg actggcgaca
     1921 gacagccggc tacaacctgg accaagtcct tgaacaggga ggtgtgtcca gtttgtttca
     1981 gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc ctgagcggtg aaaatgggct
     2041 gaagatcgac atccatgtca tcatcccgta tgaaggtctg agcggcgacc aaatgggcca
     2101 gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat catcacttta aggtgatcct
     2161 gcactatggc acactggtaa tcgacggggt tacgccgaac atgatcgact atttcggacg
     2221 gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc actgtaacag ggaccctgtg
     2281 gaacggcaac aaaattatcg acgagcgcct gatcaacccc gacggctccc tgctgttccg
     2341 agtaaccatc aacggagtga ccggctggcg gctgtgcgaa cgcattctgg cgtgaggccg
     2401 gccgcttcga gcagacatga tctagaggcc tgcagggctg atcagcctcg actgtgcctt
     2461 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     2521 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     2581 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     2641 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggataag atacattgat
     2701 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
     2761 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     2821 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcgcttgg
     2881 taccgtcttt ccagtggtta attaaataac ttcgtatagc atacattata cgaagttatg
     2941 gatcgtccat gctagcatta gaaagcttct agagggcccg tttaaacccg ctgatcagcc
     3001 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     3061 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     3121 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     3181 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     3241 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     3301 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     3361 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     3421 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     3481 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     3541 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     3601 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     3661 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     3721 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     3781 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3841 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3901 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3961 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     4021 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     4081 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag
     4141 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg
     4201 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca
     4261 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc
     4321 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg
     4381 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg
     4441 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta
     4501 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag
     4561 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac
     4621 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg
     4681 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg
     4741 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg
     4801 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg
     4861 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg
     4921 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc
     4981 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     5041 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     5101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     5161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5221 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     5281 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     5341 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     5401 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     5461 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     5521 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     5581 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     5641 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5701 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5761 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5821 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     5881 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     5941 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6001 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6061 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     6121 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     6181 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6241 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6301 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6361 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6421 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6481 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6541 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6601 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6661 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6721 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6781 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6841 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     6901 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     6961 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7021 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7081 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7141 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7201 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7261 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7321 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7381 atttccccga aaagtgccac ctgacgtc
//