pLVX-DD-ZsGreen1 Reporter

价格:3500元

联系方式:I47-825O-882O

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载体基本信息

出品公司: Clontech
载体名称: pLVX-DD-ZsGreen1 Reporter
质粒类型: 慢病毒载体;无启动子载体;荧光报告载体
高拷贝/低拷贝: 高拷贝
克隆方法: 限制性内切酶,多克隆位点
启动子:
载体大小: 8500 bp
5' 测序引物及序列: --
3' 测序引物及序列: --
载体标签: DD-ZsGreen1 (C-端)
载体抗性: 氨苄青霉素
筛选标记: 嘌呤霉素(Puromycin)
克隆菌株: Stbl3
宿主细胞(系): 常规细胞系(293、CV-1、CHO等)
备注: 慢病毒载体pLVX-DD-ZsGreen1 Reporter  缺少启动子元件,用于研究启动子及其它顺式转录元件在细胞内的活性;
DD-ZsGreen1与ZsGreen1相比,N端融合了destabilization domain (DD)去稳定结构域,导致ZsGreen1在细胞内很快被蛋白酶降解,降低了背景荧光,是研究启动子活性的理想报告基因。
产品目录号: 631752
稳定性: 稳表达
组成型/诱导型: --
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLVX-DD-ZsGreen1 Reporter载体图谱



pLVX-DD-ZsGreen1 Reporter 多克隆位点

pLVX-DD-ZsGreen1 Reporter 载体特征

载体简介

载体序列

LOCUS       pLVX-DD-ZsGreen1_Reporter   8454 bp    DNA   circular             6-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT     Cat. No. 631751,631752
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label=5'\LTR
     misc_feature    685..822
                     /label=packaging\signal
     misc_feature    4422..5013
                     /label=WPRE
     rep_origin      complement(6322..6995)
                     /label=pUC\origin
     CDS             complement(7140..8136)
                     /label=Amp(R)
     misc_binding    1303..1536
                     /label=RRE
     misc_binding    636..653
                     /label=PBS
     misc_feature    2028..2151
                     /label=cPPT/CTS
     LTR             5216..5852
                     /label=3'\LTR
     misc_feature    3280..3788
                     /label=PGK\Promoter
     misc_feature    3809..4408
                     /label=Puro(R)
     misc_feature    2195..2234
                     /label=MCS
     CDS             2247..2570
                     /label=DD
     CDS             2577..3269
                     /label=ZsGreen1
BASE COUNT     2089 a      2198 c      2253 g      1914 t 
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 
     2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg 
     2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag 
     2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg 
     2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag 
     2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga 
     2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca 
     2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg 
     2581 cccagtccaa gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg 
     2641 tggacggcca caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc 
     2701 aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt 
     2761 ctgccgcctt catgtacggc aaccgcgtgt tcaccgagta tccccaggac atcgtcgact 
     2821 acttcaagaa ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg 
     2881 gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc 
     2941 acgagtccaa gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga 
     3001 ccgacaactg ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga 
     3061 agggcgacgt gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg 
     3121 acaccgtgta caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc 
     3181 acaagctgac ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc 
     3241 acgccatcgc ctccggctcc gccttgccct agtctagata attctaccgg gtaggggagg 
     3301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct 
     3361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc 
     3421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc 
     3481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc 
     3541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc 
     3601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg 
     3661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg 
     3721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg 
     3781 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc 
     3841 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc 
     3901 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga 
     3961 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc 
     4021 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat 
     4081 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg 
     4141 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc 
     4201 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc 
     4261 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta 
     4321 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg 
     4381 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca 
     4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 
     4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 
     4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 
     4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 
     4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 
     4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 
     4801 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga 
     4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 
     4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 
     4981 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga 
     5041 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct 
     5101 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc 
     5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga 
     5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca 
     5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac 
     5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa 
     5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag 
     5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct 
     5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg 
     5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg 
     5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct 
     5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 
     5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt 
     5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga 
     6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc 
     6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc 
     6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc 
     6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 
     6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 
     6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 
     6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 
     6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 
     6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 
     6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt 
     6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 
     6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 
     6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 
     6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc 
     6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 
     6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 
     6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 
     7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 
     7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 
     7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 
     7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 
     7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 
     7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 
     7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 
     7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 
     7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 
     7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 
     7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 
     7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 
     7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 
     7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 
     7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 
     7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 
     7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 
     8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 
     8101 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt 
     8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 
     8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 
     8281 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc 
     8341 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt 
     8401 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt 
//

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