| 出品公司: | Clontech |
|---|---|
| 载体名称: | pLVX-DD-ZsGreen1 Reporter |
| 质粒类型: | 慢病毒载体；无启动子载体；荧光报告载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 8500 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | DD-ZsGreen1 (C-端） |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 嘌呤霉素（Puromycin） |
| 克隆菌株: | Stbl3 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 备注: |
慢病毒载体pLVX-DD-ZsGreen1 Reporter 缺少启动子元件，用于研究启动子及其它顺式转录元件在细胞内的活性； DD-ZsGreen1与ZsGreen1相比，N端融合了destabilization domain (DD)去稳定结构域，导致ZsGreen1在细胞内很快被蛋白酶降解，降低了背景荧光，是研究启动子活性的理想报告基因。 |
| 产品目录号: | 631752 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | 慢病毒 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-DD-ZsGreen1_Reporter 8454 bp DNA circular 6-NOV-2009
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
COMMENT Cat. No. 631751,631752
FEATURES Location/Qualifiers
LTR 1..635
/label=5'\LTR
misc_feature 685..822
/label=packaging\signal
misc_feature 4422..5013
/label=WPRE
rep_origin complement(6322..6995)
/label=pUC\origin
CDS complement(7140..8136)
/label=Amp(R)
misc_binding 1303..1536
/label=RRE
misc_binding 636..653
/label=PBS
misc_feature 2028..2151
/label=cPPT/CTS
LTR 5216..5852
/label=3'\LTR
misc_feature 3280..3788
/label=PGK\Promoter
misc_feature 3809..4408
/label=Puro(R)
misc_feature 2195..2234
/label=MCS
CDS 2247..2570
/label=DD
CDS 2577..3269
/label=ZsGreen1
BASE COUNT 2089 a 2198 c 2253 g 1914 t
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgtcgagctc aagcttcgaa ttctgcagtc gacggtaccg
2221 cgggcccggg atcccgcgac tctagaatgg gagtgcaggt ggaaaccatc tccccaggag
2281 acgggcgcac cttccccaag cgcggccaga cctgtgtggt gcactacacc gggatgcttg
2341 aagatggaaa gaaagtcgat tcctcccggg acagaaacaa gccctttaag tttatgctag
2401 gcaagcagga ggtgatccga ggctgggaag aaggggttgc ccagatgagt gtgggtcaga
2461 gagccaaact gactatatct ccagattatg cctatggtgc cactgggcac ccaggcatca
2521 tcccaccaca tgccactctc gtcttcgatg tggagcttct aaaaccggaa agatctatgg
2581 cccagtccaa gcacggcctg accaaggaga tgaccatgaa gtaccgcatg gagggctgcg
2641 tggacggcca caagttcgtg atcaccggcg agggcatcgg ctaccccttc aagggcaagc
2701 aggccatcaa cctgtgcgtg gtggagggcg gccccttgcc cttcgccgag gacatcttgt
2761 ctgccgcctt catgtacggc aaccgcgtgt tcaccgagta tccccaggac atcgtcgact
2821 acttcaagaa ctcctgcccc gccggctaca cctgggaccg ctccttcctg ttcgaggacg
2881 gcgccgtgtg catctgcaac gccgacatca ccgtgagcgt ggaggagaac tgcatgtacc
2941 acgagtccaa gttctacggc gtgaacttcc ccgccgacgg ccccgtgatg aagaagatga
3001 ccgacaactg ggagccctcc tgcgagaaga tcatccccgt gcccaagcag ggcatcttga
3061 agggcgacgt gagcatgtac ctgctgctga aggacggtgg ccgcttgcgc tgccagttcg
3121 acaccgtgta caaggccaag tccgtgcccc gcaagatgcc cgactggcac ttcatccagc
3181 acaagctgac ccgcgaggac cgcagcgacg ccaagaacca gaagtggcac ctgaccgagc
3241 acgccatcgc ctccggctcc gccttgccct agtctagata attctaccgg gtaggggagg
3301 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
3361 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
3421 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
3481 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
3541 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
3601 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
3661 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
3721 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
3781 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
3841 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
3901 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
3961 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
4021 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
4081 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
4141 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
4201 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
4261 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
4321 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
4381 gtgcatgacc cgcaagcccg gtgcctgacc gcgtctggaa caatcaacct ctggattaca
4441 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
4501 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4561 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
4621 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
4681 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
4741 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
4801 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
4861 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
4921 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
4981 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga
5041 aaaacatgga gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct
5101 agaagcacaa gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc
5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
6961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
7021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
7081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
7141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
7201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
7261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
7321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
7381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
7441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
7501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
7561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
7621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
7681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
7741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
7801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
7861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
7921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
7981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
8041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
8101 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tcaacttgtt
8161 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
8221 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
8281 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
8341 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
8401 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
