| 质粒名称: | pCDNA3.1-Nanog |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 28221 |
| 存储实验室: | Linzhao Cheng |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 5428 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | iNanog |
| 物种来源: | H、 智者（人类） |
| 插入大小: | 918 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | unknown (unknown if destroyed) |
| 3' 克隆位点: | unknown (unknown if destroyed) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pCDNA3.1-Nanog was a gift from Linzhao Cheng (Addgene plasmid # 28221 ; http://n2t.net/addgene:28221 ; RRID:Addgene_28221) |
| 参考文献部分: | Efficient human iPS cell derivation by a non-integrating plasmid from blood cells with unique epigenetic and gene expression signatures. Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA, Tong J, Cheng L. Cell Res. 2011 Jan 18. ():. 10.1038/cr.2011.12 PubMed 21243013 |
买家导航
pCDNA3.1Nanog载体基本信息
pCDNA3.1Nanog载体图谱质粒图谱和多克隆位点信息
pCDNA3.1Nanog载体简介
pCDNA3.1Nanog载体序列
LOCUS pCDNA3.1-Nanog 6803 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCDNA3.1-Nanog
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6803)
AUTHORS Chou BK, Mali P, Huang X, Ye Z, Dowey SN, Resar LM, Zou C, Zhang YA,
Tong J, Cheng L
TITLE Efficient human iPS cell derivation by a non-integrating plasmid
from blood cells with unique epigenetic and gene expression
signatures.
JOURNAL Cell Res. 2011 Jan 18. ():.
PUBMED 21243013
REFERENCE 2 (bases 1 to 6803)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6803
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 2031..2142
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
polyA_signal 2231..2455
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2501..2929
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2588..2607)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 2798..2819
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(2938..2958)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 2943..3272
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3123..3258
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3185..3204
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3321..4346
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
polyA_signal 4476..4597
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4513..4532)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4567..4586
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4646..4662)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4646..4662)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4659..4681)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4670..4686
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4694..4724)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4739..4760
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4877..4894)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5048..5636)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5128..5147)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5807..6667)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6430..6449
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6668..6772)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaacgg gccctctagt aacggccgcc agtgtgctgg aattcgccct tgtggatcca
961 tgagtgtgga tccagcttgt ccccaaagct tgccttgctt tgaagcatcc gactgtaaag
1021 aatcttcacc tatgcctgtg atttgtgggc ctgaagaaaa ctatccatcc ttgcaaatgt
1081 cttctgctga gatgcctcac acggagactg tctctcctct tccctcctcc atggatctgc
1141 ttattcagga cagccctgat tcttccacca gtcccaaagg caaacaaccc acttctgcag
1201 agaatagtgt cgcaaaaaag gaagacaagg tcccagtcaa gaaacagaag accagaactg
1261 tgttctcttc cacccagctg tgtgtactca atgatagatt tcagagacag aaatacctca
1321 gcctccagca gatgcaagaa ctctccaaca tcctgaacct cagctacaaa caggtgaaga
1381 cctggttcca gaaccagaga atgaaatcta agaggtggca gaaaaacaac tggccgaaga
1441 atagcaatgg tgtgacgcag aaggcctcag cacctaccta ccccagcctc tactcttcct
1501 accaccaggg atgcctggtg aacccgactg ggaaccttcc aatgtggagc aaccagacct
1561 ggaacaattc aacctggagc aaccagaccc agaacatcca gtcctggagc aaccactcct
1621 ggaacactca gacctggtgc acccaatcct ggaacaatca ggcctggaac agtcccttct
1681 ataactgtgg agaggaatct ctgcagtcct gcatgcagtt ccagccaaat tctcctgcca
1741 gtgacttgga ggctgctttg gaagctgctg gggaaggcct taatgtaata cagcagacca
1801 ctaggtattt tagtactcca caaaccatgg atttattcct aaactactcc atgaacatgc
1861 aacctgaaga cgtgtgagga tccgtaaggg cgaattctgc agatatccat cacactggcg
1921 gccgctcgag cggccgccac tgtgctggat atctgcagaa ttccaccaca ctggactagt
1981 ggatccgagc tcggtaccaa gcttaagttt aaaccgctga tcagcctcga ctgtgccttc
2041 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
2101 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
2161 tcattccaca ctggactagt ggatccgagc tcggtaccaa gcttaagttt aaaccgctga
2221 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
2281 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
2341 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
2401 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
2461 gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca
2521 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
2581 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
2641 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
2701 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
2761 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
2821 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
2881 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
2941 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
3001 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
3061 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
3121 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
3181 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
3241 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
3301 tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
3361 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
3421 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
3481 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
3541 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
3601 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
3661 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
3721 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
3781 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
3841 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
3901 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
3961 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
4021 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
4081 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
4141 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
4201 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
4261 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
4321 gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc accgccgcct
4381 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
4441 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
4501 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
4561 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
4621 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
4681 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
4741 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
4801 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
4861 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
4921 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
4981 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
5041 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
5101 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
5161 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
5221 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
5281 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
5341 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
5401 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
5461 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
5521 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
5581 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
5641 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5701 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5761 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5821 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5881 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5941 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
6001 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
6061 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
6121 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
6181 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
6241 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
6301 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
6361 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
6421 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
6481 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
6541 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
6601 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6661 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6721 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6781 cccgaaaagt gccacctgac gtc
//
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