miniCGBE1-SpRY(pBM1571)

LOCUS       miniCGBE1-SpRY(pBM1571)       9195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CMV promoter expression plasmid for 
            rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald
            J, Joung JK
  TITLE     CRISPR C-to-G base editors for inducing targeted DNA transversions 
            in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 
            10.1038/s41587-020-0609-x.
  PUBMED    32690971
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 
            10.1038/s41587-020-0609-x."
COMMENT     Sequence Label: miniCGBE1-SpRY (pBM1571)
FEATURES             Location/Qualifiers
     source          1..9195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(510..1301,1302..1370))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1133..1152
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1371..1475)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1554..1573)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1745..2124
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2125..2328
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2279..2299
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2370..2389
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2370..2388
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2438..2458
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3239..7339
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7382..7402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7421..7477
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             join(7478..7480,7481..7483,7484..8197)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(7478..7480,7481..7483,7484..8194)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7784..7803)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8131..8152
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8203..8220
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8243..8260)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8249..8473
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(8544..8560)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8544..8560)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8557..8579)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8568..8584
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8592..8598,8599..8616,8617..8622))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8637..8658
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8775..8792)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(8946..9195,1..339))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9026..9045)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
       61 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      121 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      181 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      241 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      301 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      361 acggggtctg acactcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      421 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
      481 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
      541 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
      601 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
      661 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
      721 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
      781 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
      841 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
      901 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
      961 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1021 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1081 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1141 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     1201 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     1261 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     1321 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     1381 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     1441 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     1501 gacgtcgacg gatcgggaga tcgatctccc gatcccctag ggtcgactct cagtacaatc
     1561 tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct
     1621 gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg
     1681 aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg
     1741 cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
     1801 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
     1861 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     1921 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     1981 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2041 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2101 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
     2161 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
     2221 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
     2281 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
     2341 cgtcagatcc gctagagatc cgcggccgct aatacgactc actataggga gagccgccac
     2401 catgaaacgg acagccgacg gaagcgagtt cgagtcacca aagaagaagc ggaaagtctc
     2461 ctcagagact gggcctgtcg ccgtcgatcc aaccctgcgc cgccggattg aacctcacga
     2521 gtttgaagtg ttctttgacc cccgggagct ggccaaggag acatgcctgc tgtacgagat
     2581 caactgggga ggcaggcact ccatctggag gcacacctct cagaacacaa ataagcacgt
     2641 ggaggtgaac ttcatcgaga agtttaccac agagcggtac ttctgcccca ataccagatg
     2701 tagcatcaca tggtttctga gctggtcccc ttgcggagag tgtagcaggg ccatcaccga
     2761 gttcctgtcc agatatccac acgtgacact gtttatctac atcgccaggc tgtatcacca
     2821 cgcagaccca aggaataggc agggcctgcg cgatctgatc agctccggcg tgaccatcca
     2881 gatcatgaca gagcaggagt ccggctactg ctggcggaac ttcgtgaatt attctcctag
     2941 caacgaggcc cactggccta ggtacccaca cctgtgggtg cgcctgtacg tgctggagct
     3001 gtattgcatc atcctgggcc tgcccccttg tctgaatatc ctgcggagaa agcagcccca
     3061 gctgaccttc tttacaatcg ccctgcagtc ttgtcactat cagaggctgc caccccacat
     3121 cctgtgggcc acaggcctga agtctggagg atctagcgga ggatcctctg gcagcgagac
     3181 accaggaaca agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcga
     3241 caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac
     3301 cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag
     3361 catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgagagaac
     3421 ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct
     3481 gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga
     3541 agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat
     3601 cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact
     3661 ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat
     3721 caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga
     3781 caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa
     3841 cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct
     3901 ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat
     3961 tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc
     4021 caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat
     4081 cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct
     4141 gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat
     4201 caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca
     4261 gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta
     4321 cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa
     4381 gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca
     4441 gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat
     4501 tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa
     4561 gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt
     4621 cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt
     4681 ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct
     4741 gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa
     4801 cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg
     4861 cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa
     4921 gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg
     4981 cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa
     5041 ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac
     5101 cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct
     5161 gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct
     5221 gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt
     5281 cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct
     5341 gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga
     5401 gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa
     5461 ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga
     5521 aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa
     5581 gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga
     5641 aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat
     5701 gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt
     5761 gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa
     5821 gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta
     5881 ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa
     5941 ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt
     6001 ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa
     6061 gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct
     6121 ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca
     6181 ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc
     6241 taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat
     6301 cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat
     6361 catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct
     6421 gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac
     6481 cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac
     6541 aggcggcttc agcaaagagt ctatcagacc caagaggaac agcgataagc tgatcgccag
     6601 aaagaaggac tgggacccta agaagtacgg cggcttcctg tggcccaccg tggcctattc
     6661 tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga
     6721 gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct
     6781 ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc
     6841 cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccaagc agctgcagaa
     6901 gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta
     6961 tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca
     7021 caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct
     7081 ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat
     7141 cagagagcag gccgagaata tcatccacct gtttaccctg accagactgg gagcccctag
     7201 agccttcaag tactttgaca ccaccatcga ccccaagcag tacagaagca ccaaagaggt
     7261 gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct
     7321 gtctcagctg ggaggtgact ctggcggctc aaaaagaacc gccgacggca gcgaattcga
     7381 gcccaagaag aagaggaaag tcggaggcgg tggaagcggc gcaacaaact tctctctgct
     7441 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gtgagcaagg gcgaggagct
     7501 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     7561 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     7621 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     7681 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     7741 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     7801 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     7861 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     7921 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     7981 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     8041 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     8101 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     8161 cgggatcact ctcggcatgg acgagctgta caagtgaccg gtcatcatca ccatcaccat
     8221 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     8281 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     8341 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     8401 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     8461 gtgggctcta tggcttctga ggcggaaaga accagctggg gctcgatacc gtcgacctct
     8521 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8581 acaattccac acaacatacg agccggaagc ataaagtgta aagcctaggg tgcctaatga
     8641 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8701 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8761 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8821 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8881 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8941 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9001 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9061 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9121 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9181 cgctccaagc tgggc
//