| 质粒名称: | pLH-sgRNA1-PP7-boxB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75393 |
| 存储实验室: | Thoru Pederson |
|
载体骨架基本信息 |
|
| 载体名称: | pLKO.1 |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8000 bp |
| 完整质粒大小: | 8318 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | sgRNA1-2XPP7-boxB |
| 物种来源: | Synthetic |
| 插入大小: | 116 bp |
| 启动子 | U6 |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Sbf I(未破坏) |
| 3' 克隆位点: | EcoR I (未破坏) |
| 5' 测序引物 | cccttcaccgagggcctatttccc |
| 3' 测序引物 | CTATTCTTTCCCCTGCACTGTACCC |
|
文献引用方法 |
|
| 材料和方法部分: | pLH-sgRNA1-PP7-boxB was a gift from Thoru Pederson (Addgene plasmid # 75393 ; http://n2t.net/addgene:75393 ; RRID:Addgene_75393) |
| 参考文献部分: | Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T. Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526. 10.1038/nbt.3526 PubMed 27088723 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLH-sgRNA1-PP7-boxB 8307 bp ds-DNA circular SYN 11-FEB-2020
DEFINITION sgRNA1-PP7-boxB.
ACCESSION .
VERSION .
KEYWORDS pLH-sgRNA1-PP7-boxB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8307)
AUTHORS Ma H, Tu LC, Naseri A, Huisman M, Zhang S, Grunwald D, Pederson T
TITLE Multiplexed labeling of genomic loci with dCas9 and engineered
sgRNAs using CRISPRainbow.
JOURNAL Nat Biotechnol. 2016 Apr 18. doi: 10.1038/nbt.3526.
PUBMED 27088723
REFERENCE 2 (bases 1 to 8307)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 11, 2020 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8307
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 123..142
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 376..393
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 510..531
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 546..576
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 584..600
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 589..611
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 608..624
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 608..624
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 643..663
/label=T3
/note="T3 promoter, forward primer"
promoter 645..663
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 691..917
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 918..1098
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1145..1270
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1763..1996
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
promoter 2523..2763
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
primer_bind 2523..2543
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 2694..2713
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
CDS 3112..3417
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3296..3315
/label=ccdB-fwd
/note="ccdB gene, forward primer"
misc_feature 3602..3719
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 3768..4278
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
primer_bind 4197..4215
/label=hPGK-F
/note="Human PGK promoter, forward primer"
CDS 4368..5393
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
primer_bind complement(5417..5433)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5418..5434)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5516..5749
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5821..5942
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5858..5877)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5912..5931
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin 5982..6117
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6044..6063
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(6137..6156)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6138..6156)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6166..6183)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6166..6182)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6175..6197)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6324..6779
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6411..6430)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6621..6642
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6805..6909
/gene="bla"
/label=AmpR promoter
CDS 6910..7770
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7128..7147)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7941..222
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
61 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
601 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
661 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
721 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
781 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
841 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
901 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
961 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
1021 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
1081 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
1141 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
1201 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
1261 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
1321 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
1381 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
1441 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
1501 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
1561 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
1621 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
1681 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
1741 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
1801 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
1861 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
1921 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
1981 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
2041 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
2101 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
2161 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
2221 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
2281 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
2341 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg
2401 acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat
2461 tagtgaacgg atctcgacgg tatcgatcac gagactagcc tcgagcggcc gcccccttca
2521 ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
2581 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
2641 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
2701 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
2761 gacgaaacac cggtgtcttc gaggcttact aaaagccaga taacagtatg cgtatttgcg
2821 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
2881 aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
2941 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
3001 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
3061 ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga aatgcagttt
3121 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
3181 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
3241 gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
3301 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
3361 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaacc
3421 ctgcaggacg aagacccgtt tgagagctac cggagcagac gatatggcgt cgctccggta
3481 gcaagttcaa ataaggctag tccgttatca acttgggccc tgaagaaggg cccaagtggc
3541 accgagtcgg tgcttttttt gaattctcga cctcgagaca aatggcagta ttcatccaca
3601 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
3661 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
3721 gggtttatta cagggacagc agagatccac tttggccgcg gctcgagggg gttggggttg
3781 cgccttttcc aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc
3841 gggaaacgca gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc
3901 acccggatct tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta
3961 agtcgggaag gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt
4021 ctcactagta ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga
4081 tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg
4141 gcggtgcggg aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt
4201 tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc
4261 accgacctct ctccccaggg ggatccaccg gatcaagctt gccagctggg gcgccctctg
4321 gtaaggttgg gaagccctgc agatctgatc ccggggggca atgagatatg aaaaagcctg
4381 aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc
4441 tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg
4501 gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc
4561 ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg gaatttagcg
4621 agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gatctgcctg
4681 aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg
4741 ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca
4801 ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg
4861 tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg
4921 ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc
4981 tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt
5041 cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc
5101 agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt
5161 atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg
5221 atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg
5281 ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac
5341 tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa tagagtagat
5401 gccgaccggg atctatcgat accgtcgacc tcgagggggg gcccggtacc tttaagacca
5461 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
5521 gggctaattc actcccaacg aagaaaagat ctgctttttg cttgtactgg gtctctctgg
5581 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
5641 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
5701 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
5761 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
5821 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
5881 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
5941 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
6001 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
6061 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
6121 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
6181 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
6241 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
6301 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
6361 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
6421 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
6481 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
6541 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
6601 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
6661 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
6721 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
6781 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
6841 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
6901 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
6961 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
7021 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
7081 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
7141 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
7201 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
7261 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
7321 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
7381 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
7441 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
7501 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
7561 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
7621 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
7681 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
7741 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
7801 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
7861 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
8281 cccagcttgg agcgaacgac ctacacc
//
