pCytERM-mfRFP2-N1

LOCUS       pCytERM-mfRFP2-N1               4818 bp DNA     circular SYN 22-NOV-2023
DEFINITION  Vector for fusing EGFP to the C-terminus of a partner protein. For 
            other reading frames, use pEGFP-N2 or pEGFP-N3.
ACCESSION   U55762
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4818)
  AUTHORS   Clontech (TaKaRa)
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..4818
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             615..701
                     /codon_start=1
                     /product="cytochrome P-450 IIC1, partial [Oryctolagus 
                     cuniculus]
                     Sequence ID: AAA31436.1Length: 56Number of Matches: 1"
                     /label=cytochrome P-450
                     /translation="MDPVVVLGLCLSCLLLLSLWKQSYGGGKL"
     regulatory      747..752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             753..1484
                     /codon_start=1
                     /label=mfRFP
                     /translation="MVSKGEELIKENMPLKLYMEGTVNNHHFKCTAEGEGKPYEGTQTQ
                     RIKVVEGGPLPFAFDILATSFMYGSHTFIDHTPGIPDFWKQSFPEGFTWERVTTYEDGG
                     VLIATQDTSLHDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEAHTELLYPTDGGLEGRTA
                     LALKLVGGGHLLCNFKTIYRSKKPAKNLKMPGVYYVDYRLERIEEADNETYVEQHEVAV
                     ARYCDLPTKLGHKLKGMDELYK"
     polyA_signal    1606..1727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1734..2189)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        2216..2320
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2322..2679
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2530..2665
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2714..3508
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin)"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     misc_feature    3740..3787
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     rep_origin      4116..4704
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctatggac cctgtggtgg tgctggggct ctgtctctcc tgtttgcttc
      661 tcctttcact ctggaaacag agctatgggg gagggaagct tcgaattctg cagtcgacgg
      721 taccgcgggc ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagctgatta
      781 aggagaacat gccactgaag ctgtacatgg agggcaccgt taacaatcac cacttcaagt
      841 gcacagctga gggcgaaggc aagccctacg agggcaccca gacccagaga atcaaggtgg
      901 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagtttc atgtacggca
      961 gtcacacctt catcgaccac accccgggca tccccgactt ctggaagcag tccttccccg
     1021 agggcttcac gtgggagaga gtcaccacat acgaagacgg gggcgtcctg atcgcgaccc
     1081 aggacaccag cctccatgac ggctgcctca tctacaacgt caagttgaga ggggtgaact
     1141 tcccatccaa cggccctgtg atgcaaaaga aaacactcgg ctgggaggca cacaccgagc
     1201 ttttgtaccc cactgacggc ggtctggaag gcagaaccgc gctggccctg aagcttgtgg
     1261 gcgggggcca cctattgtgt aacttcaaga ctatatacag atccaagaaa cccgctaaga
     1321 acctcaagat gcccggcgtc tactatgtgg actacagact ggaaagaatc gaggaggccg
     1381 acaatgagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc gacctcccta
     1441 ccaaactggg gcacaagctt aagggcatgg acgagctgta caaggcggcc gcgactctag
     1501 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1561 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1621 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     1681 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaaattgt
     1741 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     1801 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     1861 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     1921 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     1981 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     2041 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     2101 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     2161 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
     2221 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     2281 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
     2341 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     2401 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     2461 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     2521 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     2581 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     2641 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     2701 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     2761 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     2821 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     2881 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     2941 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     3001 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     3061 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     3121 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     3181 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     3241 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     3301 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     3361 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     3421 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     3481 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     3541 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     3601 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     3661 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     3721 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     3781 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     3841 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     3901 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     3961 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     4021 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     4081 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     4141 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     4201 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     4261 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     4321 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     4381 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     4441 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     4501 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     4561 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     4621 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     4681 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     4741 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     4801 cgtattaccg ccatgcat
//