vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR

LOCUS        vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR               8930 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  For the auxin receiver that report on auxin..
ACCESSION   .
VERSION     .
KEYWORDS    vYM073_PB-pEF-osTIR1-T2A-mCherry-AID-Flag-BlastR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8930)
  TITLE     Synthetic mammalian signaling circuits for robust cell population 
            control
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(168..756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(248..267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(927..1787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1550..1569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1788..1892)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1918..2373)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2055..2076)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2267..2286
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2500..2522
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2514..2531
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2515..2531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2661..2723
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        3166..4344
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3397..4335
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4292..4312
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4367..6091
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             6092..6145
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6146..6850
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(6288..6306)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6556..6575
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6872..7075
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Kubota et al., 2013)"
                     /label=mini-AID
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
                     AAFVKVSMDGAPYLRKIDLRMYK"
     CDS             7088..7111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7127..7525
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7527..7648
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7564..7583)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7618..7637
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     repeat_region   8171..8205
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(8696..8712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8696..8712)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8709..8731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8720..8736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8744..8774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8789..8810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8927..14)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
       61 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      121 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca
     1081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
     1921 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     1981 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     2041 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     2101 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     2161 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     2221 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     2281 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     2341 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
     2401 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     2461 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     2521 cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag gacgggcaga
     2581 ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc gacacgctgc
     2641 agaacacgca gctagattaa ccctagaaag ataatcatat tgtgacgtac gttaaagata
     2701 atcatgtgta aaattgacgc atgtgtttta tcggtctgta tatcgaggtt tatttattaa
     2761 tttgaataga tattaagttt tattatattt acacttacat actaataata aattcaacaa
     2821 acaatttatt tatgtttatt tatttattaa aaaaaacaaa aactcaaaat ttcttctata
     2881 aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga tgtaattacg
     2941 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
     3001 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
     3061 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
     3121 acggggaaaa ggcctccacg gccagcacct gcgtgacgta cgtgaggctc cggtgcccgt
     3181 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     3241 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     3301 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     3361 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     3421 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     3481 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     3541 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     3601 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     3661 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     3721 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     3781 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     3841 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     3901 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     3961 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     4021 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     4081 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     4141 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     4201 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     4261 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     4321 tttcttccat ttcaggtgtc gtgaaccttc acgcaggtgg gccaccatga catactttcc
     4381 tgaagaggtc gtcgaacaca tttttagctt cctgcctgca cagagagata gaaacacagt
     4441 gagcctggtc tgcaaagtgt ggtacgagat cgaacgcctg agccggagag gagtgttcgt
     4501 cggcaactgc tatgctgtga gagcaggcag ggtcgccgct aggtttccaa atgtgcgcgc
     4561 actgaccgtc aaggggaaac cccacttcgc cgactttaac ctggtgcccc ctgattgggg
     4621 aggatacgcc ggcccttgga tcgaggcagc cgctcgcggc tgtcatggac tggaggaact
     4681 gcgcatgaag cgaatggtgg tctctgacga aagtctggag ctgctggctc ggagcttccc
     4741 taggtttcgc gcactggtgc tgatttcttg cgaaggcttc agcaccgatg gactggcagc
     4801 cgtggcctcc cactgtaagc tgctgcggga gctggacctc caggagaatg aagtggagga
     4861 tagaggcccc agatggctgt cttgcttccc agactcatgt accagcctgg tgtccctgaa
     4921 ctttgcctgc atcaaaggcg aagtgaatgc tgggtccctg gagcggctgg tctcaagaag
     4981 ccccaacctg aggtctctgc ggctgaaccg gagcgtgagc gtggacactc tggctaagat
     5041 tctgctgaga acccctaacc tggaggatct gggaaccggc aatctgacag acgatttcca
     5101 gacagaatcc tactttaaac tgacttctgc cctggagaag tgtaaaatgc tgaggagtct
     5161 gtcaggattc tgggatgctt cacccgtgtg cctgagcttt atctaccctc tgtgtgcaca
     5221 gctgacaggc ctgaacctga gctatgcacc aaccctggac gccagtgatc tgacaaagat
     5281 gatctcacgc tgcgtgaaac tccagcgact gtgggtgctg gactgtattt ccgataaggg
     5341 gctccaggtg gtcgccagct cctgcaagga cctccaggag ctgagagtgt tcccatctga
     5401 tttttacgtg gccggatata gtgctgtcac tgaggaaggc ctggtggcag tctcactggg
     5461 atgcccaaag ctgaacagcc tgctgtattt ctgtcatcag atgactaatg ctgcactggt
     5521 gaccgtcgcc aagaactgcc ctaatttcac ccgatttcgg ctgtgtattc tggaaccagg
     5581 caaacccgac gtggtcacat cccagccact ggatgaaggg tttggagcta tcgtgagaga
     5641 gtgcaaggga ctccagaggc tgagcatttc cggcctgctg acagacaaag tgttcatgta
     5701 catcggcaag tatgctaagc agctggagat gctgagcatt gcatttgccg gagactccga
     5761 taagggcatg atgcacgtga tgaacgggtg taagaatctg cgaaaactgg aaatccggga
     5821 cagccctttc ggggatgccg ctctgctggg aaactttgcc agatacgaga caatgaggag
     5881 cctgtggatg tctagttgca atgtgactct gaagggctgt caggtcctgg ctagtaaaat
     5941 gcctatgctg aacgtggaag tcattaatga gcgggacggg tctaacgaaa tggaggaaaa
     6001 tcatggcgac ctgccaaagg tggagaaact gtatgtgtat cggaccaccg caggggcaag
     6061 agatgatgct cccaactttg tgaagattct ggagggcagg gggagcctcc taacttgcgg
     6121 agacgtcgag gagaatcctg gcccagtgag caagggcgag gaggataaca tggccatcat
     6181 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     6241 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     6301 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     6361 ctccaaggcc tacgtgaagc accccgccga cattcccgac tacttgaagc tgtccttccc
     6421 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     6481 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     6541 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     6601 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     6661 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     6721 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     6781 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     6841 gctgtacaag tctggatccg gtgcaggcgc caaggagaag agtgcttgtc ctaaagatcc
     6901 agccaaacct ccggccaagg cacaagttgt gggatggcca ccggtgagat cataccggaa
     6961 gaacgtgatg gtttcctgcc aaaaatcaag cggtggcccg gaggcggcgg cgttcgtgaa
     7021 ggtatcaatg gacggagcac cgtacttgag gaaaatcgat ttgaggatgt ataaaggagg
     7081 atccggtgac tataaggacg atgacgacaa gggtggtgga gggtccatgg ccaagccttt
     7141 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     7201 ctctgaagat tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     7261 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     7321 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     7381 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     7441 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     7501 ctctggttat gtgtgggagg gctgacaact tgtttattgc agcttataat ggttacaaat
     7561 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7621 gtttgtccaa actcatcaat gtatcttagg cccattggta tggctttttc cccgtatccc
     7681 cccaggtgtc tgcaggctca aagagcagcg agaagcgttc agaggaaagc gatcccgtgc
     7741 caccttcccc gtgcccgggc tgtccccgca cgctgccggc tcggggatgc ggggggagcg
     7801 ccggaccgga gcggagcccc gggcggctcg ctgctgcccc ctagcggggg agggacgtaa
     7861 ttacatccct gggggctttg ggggggggct gtccctgata tctataacaa gaaaatatat
     7921 atataataag ttatcacgta agtagaacat gaaataacaa tataattatc gtatgagtta
     7981 aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt gactcacgcg gtcgttatag
     8041 ttcaaaatca gtgacactta ccgcattgac aagcacgcct cacgggagct ccaagcggcg
     8101 actgagatgt cctaaatgca cagcgacgga ttcgcgctat ttagaaagag agagcaatat
     8161 ttcaagaatg catgcgtcaa ttttacgcag actatctttc tagggttaat ctagctgcat
     8221 caggatcata tcgtcgggtc ttttttccgg ctcagtcatc gcccaagctg gcgctatctg
     8281 ggcatcgggg aggaagaagc ccgtgccttt tcccgcgagg ttgaagcggc atggaaagag
     8341 tttgccgagg atgactgctg ctgcattgac gttgagcgaa aacgcacgtt taccatgatg
     8401 attcgggaag gtgtggccat gcacgccttt aacggtgaac tgttcgttca ggccacctgg
     8461 gataccagtt cgtcgcggct tttccggaca cagttccgga tggtcagccc gaagcgcatc
     8521 agcaacccga acaataccgg cgacagccgg aactgccgtg ccggtgtgca gattaatgac
     8581 agcggtgcgg cgctgggata ttacgtcagc gaggacgggt atcctggctg gatgccgcag
     8641 aaatggacat ggataccccg tgagttaccc ggcgggcgcg cttggcgtaa tcatggtcat
     8701 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8761 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8821 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8881 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
//