pAAVLINKv1-pCALM1-Cre-ATM-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-ATM-C-FLAG                7118 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 20, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7118
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3831
                     /codon_start=1
                     /gene="ATM"
                     /gene_synonym="AT1; ATA; ATC; ATD; ATDC; ATE; TEL1; TELO1"
                     /product="serine-protein kinase ATM isoform a"
                     /EC_number="2.7.11.1"
                     /label=ATM
                     /note="isoform a is encoded by transcript variant 2; TEL1, 
                     telomere maintenance 1, homolog; AT mutated; serine-protein
                     kinase ATM; A-T mutated; ataxia telangiectasia mutated; 
                     serine/threonine kinase ATM"
                     /db_xref="GI:71902540"
                     /db_xref="CCDS:CCDS31669.1"
                     /db_xref="GeneID:472"
                     /db_xref="HGNC:HGNC:795"
                     /db_xref="MIM:607585"
                     /protein_id="NP_000042.3"
                     /translation="AIFQIKQYNSVSCGVSEWQLEEAQVFWAKKEQSLALSILKQMIKK
                     LDASCAANNPSLKLTYTECLRVCGNWLAETCLENPAVIMQTYLEKAVEVAGNYDGESSD
                     ELRNGKMKAFLSLARFSDTQYQRIENYMKSSEFENKQALLKRAKEEVGLLREHKIQTNR
                     YTVKVQRELELDELALRALKEDRKRFLCKAVENYINCLLSGEEHDMWVFRLCSLWLENS
                     GVSEVNGMMKRDGMKIPTYKFLPLMYQLAARMGTKMMGGLGFHEVLNNLISRISMDHPH
                     HTLFIILALANANRDEFLTKPEVARRSRITKNVPKQSSQLDEDRTEAANRIICTIRSRR
                     PQMVRSVEALCDAYIILANLDATQWKTQRKGINIPADQPITKLKNLEDVVVPTMEIKVD
                     HTGEYGNLVTIQSFKAEFRLAGGVNLPKIIDCVGSDGKERRQLVKGRDDLRQDAVMQQV
                     FQMCNTLLQRNTETRKRKLTICTYKVVPLSQRSGVLEWCTGTVPIGEFLVNNEDGAHKR
                     YRPNDFSAFQCQKKMMEVQKKSFEEKYEVFMDVCQNFQPVFRYFCMEKFLDPAIWFEKR
                     LAYTRSVATSSIVGYILGLGDRHVQNILINEQSAELVHIDLGVAFEQGKILPTPETVPF
                     RLTRDIVDGMGITGVEGVFRRCCEKTMEVMRNSQETLLTIVEVLLYDPLFDWTMNPLKA
                     LYLQQRPEDETELHPTLNADDQECKRNLSDIDQSFNKVAERVLMRLQEKLKGVEEGTVL
                     SVGGQVNLLIQQAIDPKNLSRLFPGWKAWV"
     misc_feature    2742..2747
                     /label=SDS
     CDS             3838..3903
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3907..4338
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4051..4056
                     /label=SDS
     repeat_region   4381..4521
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4381..4510
                     /label=AAV2 ITR
     repeat_region   4382..4522
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4596..5051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5333..5437
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5438..6298
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6469..7057
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaatatt tcaaattaaa
     1501 cagtacaatt cagttagctg tggagtctct gagtggcagc tggaagaagc acaagtattc
     1561 tgggcaaaaa aggagcagag tcttgccctg agtattctca agcaaatgat caagaagttg
     1621 gatgccagct gtgcagcgaa caatcccagc ctaaaactta catacacaga atgtctgagg
     1681 gtttgtggca actggttagc agaaacgtgc ttagaaaatc ctgcggtcat catgcagacc
     1741 tatctagaaa aggcagtaga agttgctgga aattatgatg gagaaagtag tgatgagcta
     1801 agaaatggaa aaatgaaggc atttctctca ttagcccggt tttcagatac tcaataccaa
     1861 agaattgaaa actacatgaa atcatcggaa tttgaaaaca agcaagctct cctgaaaaga
     1921 gccaaagagg aagtaggtct ccttagggaa cataaaattc agacaaacag atacacagta
     1981 aaggttcagc gagagctgga gttggatgaa ttagccctgc gtgcactgaa agaggatcgt
     2041 aaacgcttct tatgtaaagc agttgaaaat tatatcaact gcttattaag tggagaagaa
     2101 catgatatgt gggtattccg actttgttcc ctctggcttg aaaattctgg agtttctgaa
     2161 gtcaatggca tgatgaagag agacggaatg aagattccaa catataaatt tttgcctctt
     2221 atgtaccaat tggctgctag aatggggacc aagatgatgg gaggcctagg atttcatgaa
     2281 gtcctcaata atctaatctc tagaatttca atggatcacc cccatcacac tttgtttatt
     2341 atactggcct tagcaaatgc aaacagagat gaatttctga ctaaaccaga ggtagccaga
     2401 agaagcagaa taactaaaaa tgtgcctaaa caaagctctc agcttgatga ggatcgaaca
     2461 gaggctgcaa atagaataat atgtactatc agaagtagga gacctcagat ggtcagaagt
     2521 gttgaggcac tttgtgatgc ttatattata ttagcaaact tagatgccac tcagtggaag
     2581 actcagagaa aaggcataaa tattccagca gaccagccaa ttactaaact taagaattta
     2641 gaagatgttg ttgtccctac tatggaaatt aaggtggacc acacaggaga atatggaaat
     2701 ctggtgacta tacagtcatt taaagcagaa tttcgcttag caggaggtgt aaatttacca
     2761 aaaataatag attgtgtagg ttccgatggc aaggagagga gacagcttgt taagggccgt
     2821 gatgacctga gacaagatgc tgtcatgcaa caggtcttcc agatgtgtaa tacattactg
     2881 cagagaaaca cggaaactag gaagaggaaa ttaactatct gtacttataa ggtggttccc
     2941 ctctctcagc gaagtggtgt tcttgaatgg tgcacaggaa ctgtccccat tggtgaattt
     3001 cttgttaaca atgaagatgg tgctcataaa agatacaggc caaatgattt cagtgccttt
     3061 cagtgccaaa agaaaatgat ggaggtgcaa aaaaagtctt ttgaagagaa atatgaagtc
     3121 ttcatggatg tttgccaaaa ttttcaacca gttttccgtt acttctgcat ggaaaaattc
     3181 ttggatccag ctatttggtt tgagaagcga ttggcttata cgcgcagtgt agctacttct
     3241 tctattgttg gttacatact tggacttggt gatagacatg tacagaatat cttgataaat
     3301 gagcagtcag cagaacttgt acatatagat ctaggtgttg cttttgaaca gggcaaaatc
     3361 cttcctactc ctgagacagt tccttttaga ctcaccagag atattgtgga tggcatgggc
     3421 attacgggtg ttgaaggtgt cttcagaaga tgctgtgaga aaaccatgga agtgatgaga
     3481 aactctcagg aaactctgtt aaccattgta gaggtccttc tatatgatcc actctttgac
     3541 tggaccatga atcctttgaa agctttgtat ttacagcaga ggccggaaga tgaaactgag
     3601 cttcacccta ctctgaatgc agatgaccaa gaatgcaaac gaaatctcag tgatattgac
     3661 cagagtttca acaaagtagc tgaacgtgtc ttaatgagac tacaagagaa actgaaagga
     3721 gtggaagaag gcactgtgct cagtgttggt ggacaagtga atttgctcat acagcaggcc
     3781 atagacccca aaaatctcag ccgacttttc ccaggatgga aagcttgggt gtgtacagac
     3841 tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac
     3901 aagtaactcg agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     3961 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4021 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg
     4081 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     4141 gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     4201 ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4261 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4321 atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc
     4381 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4441 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     4501 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     4561 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     4621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     4681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4801 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4921 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     4981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5041 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5101 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5161 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5221 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5281 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5341 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5401 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5461 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5521 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5581 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5641 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5701 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5761 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5821 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5881 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5941 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6001 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6061 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6121 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6181 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6241 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6301 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6361 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6421 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6481 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6541 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6601 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6661 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6721 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6781 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6841 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6901 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6961 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7021 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7081 acggttcctg gccttttgct ggccttttgc tcacatgt
//