pGWB11

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相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

出品公司: Research Institute of Molecular Genetics
载体名称: pGWB11
质粒类型: 植物表达载体,Gateway双基因表达载体
高拷贝/低拷贝: 高拷贝
克隆方法: Gateway连接
启动子: 35S
载体大小: 17.2 kb
5’测序引物及序列: --
3’测序引物及序列: --
载体标签:
载体抗性: 卡那霉素
筛选标记: 潮霉素hygromycin
克隆菌株: DB3.1感受态细胞
稳定性: 瞬表达
组成型/诱导型: 组成型
病毒/非病毒: 非病毒
相关文档: pGWB载体操作手册
相关质粒: pGWB4
pGWB5
pGWB11
pGWB12
pGWB17
pGWB18
pGWB21
pGWB406
pGWB453
pGWB454
pGWB455
pGWB504

载体质粒图谱和多克隆位点信息

pGWB11载体图谱
pGWB11多克隆位点

载体简介

载体序列

LOCUS       pGWB11               17260 bp    DNA     circular SYN 16-AUG-2007
DEFINITION  Gateway binary vector pGWB11 DNA, complete sequence.
ACCESSION   AB289774
VERSION     AB289774.1
KEYWORDS    .
SOURCE      Gateway binary vector pGWB11
  ORGANISM  Gateway binary vector pGWB11
            other sequences; artificial sequences; vectors.
REFERENCE   1
  AUTHORS   Nakagawa,T., Kurose,T., Hino,T., Tanaka,K., Kawamukai,M., Niwa,Y.,
            Toyooka,K., Matsuoka,K., Jinbo,T. and Kimura,T.
  TITLE     Development of series of gateway binary vectors, pGWBs, for
            realizing efficient construction of fusion genes for plant
            transformation
  JOURNAL   J. Biosci. Bioeng. 104 (1), 34-41 (2007)
   PUBMED   17697981
REFERENCE   2  (bases 1 to 17260)
  AUTHORS   Nakagawa,T. and Kimura,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2007) Tsuyoshi Nakagawa, Shimane University,
            Center for Integrated Research in Science; 1060 Nishikawatsu,
            Matsue, Shimane 690-8504, Japan
            (E-mail:tnakagaw@life.shimane-u.ac.jp, Tel:81-852-32-6595,
            Fax:81-852-32-6109)
COMMENT     constructed using pBI121.
FEATURES             Location/Qualifiers
     source          1..17260
                     /organism="Gateway binary vector pGWB11"
                     /mol_type="other DNA"
                     /db_xref="taxon:419555"
     misc_feature    complement(2454..2478)
                     /note="T-DNA right border"
     regulatory      2519..2825
                     /regulatory_class="promoter"
                     /note="NOS promoter"
     gene            2838..3632
                     /gene="nptII"
     CDS             2838..3632
                     /gene="nptII"
                     /codon_start=1
                     /transl_table=11
                     /product="neomycin phosphotransferase II"
                     /protein_id="BAF44989.1"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     regulatory      4022..4277
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     regulatory      4974..5808
                     /regulatory_class="promoter"
                     /note="35S promoter"
     misc_feature    complement(5828..5952)
                     /note="attR1"
     gene            6073..6732
                     /gene="cat"
     CDS             6073..6732
                     /gene="cat"
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="BAF44990.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            7074..7379
                     /gene="ccdB"
     CDS             7074..7379
                     /gene="ccdB"
                     /codon_start=1
                     /transl_table=11
                     /protein_id="BAF44991.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     misc_feature    7420..7544
                     /note="attR2"
     misc_feature    7555..7578
                     /note="FLAG"
     regulatory      7600..7852
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     regulatory      complement(8281..8533)
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     gene            complement(9001..10026)
                     /gene="hpt"
     CDS             complement(9001..10026)
                     /gene="hpt"
                     /codon_start=1
                     /transl_table=11
                     /product="hygromycin phosphotransferase"
                     /protein_id="BAF44992.1"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
                     LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
                     HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
                     EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
                     SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
                     TRPRAKE"
     regulatory      complement(10030..10468)
                     /regulatory_class="promoter"
                     /note="35S promoter"
     misc_feature    complement(11123..11148)
                     /note="T-DNA left border"
ORIGIN      
        1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
       61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
      121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
      181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
      241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
      301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
      361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
      421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
      481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
      541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
      601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
      661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
      721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
      781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
      841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
     1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
     1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
     1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
     1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
     1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
     1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
     1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
     1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
     1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
     1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
     1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
     1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
     1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
     1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
     2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
     2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
     2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
     2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
     2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
     2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
     2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
     2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
     2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
     2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
     2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
     2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
     2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
     2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
     3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
     3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
     3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
     3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
     4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
     4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
     4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
     4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
     4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
     4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
     4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
     4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
     4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc cccagattag
     4981 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
     5041 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
     5101 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
     5161 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
     5221 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
     5281 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
     5341 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
     5401 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
     5461 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
     5521 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
     5581 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
     5641 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
     5701 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
     5761 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
     5821 gttatcaaca agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa
     5881 tatattaaat tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat
     5941 ccagtcacta tggcggcccc tcgaggggcc gcattaggca ccccaggctt tacactttat
     6001 gcttccggct cgtataatgt gtggattttg agttaggatc cggcgagatt ttcaggagct
     6061 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     6121 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     6181 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     6241 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     6301 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     6361 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     6421 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     6481 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     6541 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     6601 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
     6661 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     6721 ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     6781 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     6841 agaggtgtgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     6901 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     6961 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     7021 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt
     7081 ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     7141 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     7201 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     7261 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     7321 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     7381 tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg gatatgttgt
     7441 gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat attgatattt
     7501 atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggttgataa cagcgactac
     7561 aaggatgacg atgacaaggc ttagagctcg aatttccccg atcgttcaaa catttggcaa
     7621 taaagtttct taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg
     7681 ttgaattacg ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg
     7741 gtttttatga ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag
     7801 cgcgcaaact aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta
     7861 gcttcatcaa cgcaagacat gcgcacgacc gtctgacagg agaggaattt ccgacgagca
     7921 cagaaaggac ttgctcttgg acgtaggcct atttctcagg cacatgtatc aagtgttcgg
     7981 acgtgggttt tcgatggtgt atcagccgcc gccaactggg agatgaggag gctttcttgg
     8041 ggggcagtca gcagttcatt tcacaagaca gaggaacttg taaggagatg cactgattta
     8101 tcttggcgca aaccagcagg acgaattagt gggaatagcc cgcgaatatc taagttatgc
     8161 ctgtcggcat gagcagaaac ttccaattcg aaacagtttg gagaggttgt ttttgggcat
     8221 accttttgtt agtcagcctc tcgattgctc atcgtcatta cacagtaccg aagtttgatc
     8281 gatctagtaa catagatgac accgcgcgcg ataatttatc ctagtttgcg cgctatattt
     8341 tgttttctat cgcgtattaa atgtataatt gcgggactct aatcataaaa acccatctca
     8401 taaataacgt catgcattac atgttaatta ttacatgctt aacgtaattc aacagaaatt
     8461 atatgataat catcgcaaga ccggcaacag gattcaatct taagaaactt tattgccaaa
     8521 tgtttgaacg atctgcttcg acgcactcct tctttactcc accatctcgt ccttattgaa
     8581 aacgtgggta gcaccaaaac gaatcaagtc gctggaactg aagttaccaa tcacgctgga
     8641 tgatttgcca gttggattaa tcttgccttt ccccgcatga ataatattga tgaatgcatg
     8701 cgtgaggggt atttcgattt tggcaatagc tgcaattgcc gcgacatcct ccaacgagca
     8761 taattcttca gaaaaatagc gatgttccat gttgtcaggg catgcatgat gcacgttatg
     8821 aggtgacggt gctaggcagt attccctcaa agtttcatag tcagtatcat attcatcatt
     8881 gcattcctgc aagagagaat tgagacgcaa tccacacgct gcggcaacct tccggcgttc
     8941 gtggtctatt tgctcttgga cgttgcaaac gtaagtgttg gatcccggtc ggcatctact
     9001 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     9061 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     9121 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     9181 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     9241 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     9301 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     9361 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     9421 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     9481 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     9541 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     9601 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     9661 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     9721 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     9781 ttccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     9841 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     9901 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     9961 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
    10021 tttcatatcg gggtcgtcct ctccaaatga aatgaacttc cttatataga ggaagggtct
    10081 tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag tggagatatc acatcaatcc
    10141 acttgctttg aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg
    10201 ggtccatctt tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg
    10261 caatgatggc atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag
    10321 atagctgggc aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca
    10381 atagcccttt ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg
    10441 tgctccacca tgttgacgga tctctaggac gcgtcctaga agctaattca ctggccgtcg
    10501 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    10561 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    10621 agttgcgcag cctgaatggc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
    10681 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
    10741 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
    10801 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
    10861 ctcttgttcc aaactggaac aacactcaac cctatctcgg gctattcttt tgatttataa
    10921 gggattttgc cgatttcgga accaccatca aacaggattt tcgcctgctg gggcaaacca
    10981 gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc
    11041 ccgtctcact ggtgaaaaga aaaaccaccc cagtacatta aaaacgtccg caatgtgtta
    11101 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
    11161 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
    11221 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
    11281 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
    11341 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaaata tcatctccct
    11401 cgcagagatc cgaattatca gccttcttat tcatttctcg cttaaccgtg acaggctgtc
    11461 gatcttgaga actatgccga cataatagga aatcgctgga taaagccgct gaggaagctg
    11521 agtggcgcta tttctttaga agtgaacgtt gacgatatca actcccctat ccattgctca
    11581 ccgaatggta caggtcgggg acccgaagtt ccgactgtcg gcctgatgca tccccggctg
    11641 atcgacccca gatctggggc tgagaaagcc cagtaaggaa acaactgtag gttcgagtcg
    11701 cgagatcccc cggaaccaaa ggaagtaggt taaacccgct ccgatcaggc cgagccacgc
    11761 caggccgaga acattggttc ctgtaggcat cgggattggc ggatcaaaca ctaaagctac
    11821 tggaacgagc agaagtcctc cggccgccag ttgccaggcg gtaaaggtga gcagaggcac
    11881 gggaggttgc cacttgcggg tcagcacggt tccgaacgcc atggaaaccg cccccgccag
    11941 gcccgctgcg acgccgacag gatctagcgc tgcgtttggt gtcaacacca acagcgccac
    12001 gcccgcagtt ccgcaaatag cccccaggac cgccatcaat cgtatcgggc tacctagcag
    12061 agcggcagag atgaacacga ccatcagcgg ctgcacagcg cctaccgtcg ccgcgacccc
    12121 gcccggcagg cggtagaccg aaataaacaa caagctccag aatagcgaaa tattaagtgc
    12181 gccgaggatg aagatgcgca tccaccagat tcccgttgga atctgtcgga cgatcatcac
    12241 gagcaataaa cccgccggca acgcccgcag cagcataccg gcgacccctc ggcctcgctg
    12301 ttcgggctcc acgaaaacgc cggacagatg cgccttgtga gcgtccttgg ggccgtcctc
    12361 ctgtttgaag accgacagcc caatgatctc gccgtcgatg taggcgccga atgccacggc
    12421 atctcgcaac cgttcagcga acgcctccat gggctttttc tcctcgtgct cgtaaacgga
    12481 cccgaacatc tctggagctt tcttcagggc cgacaatcgg atctcgcgga aatcctgcac
    12541 gtcggccgct ccaagccgtc gaatctgagc cttaatcaca attgtcaatt ttaatcctct
    12601 gtttatcggc agttcgtaga gcgcgccgtg cgtcccgagc gatactgagc gaagcaagtg
    12661 cgtcgagcag tgcccgcttg ttcctgaaat gccagtaaag cgctggctgc tgaaccccca
    12721 gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc attgacccag
    12781 gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac ctgctcgcgc
    12841 cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc cagcttgagc
    12901 gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt cggcgacagc
    12961 ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag cacgacgatt
    13021 tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag gacgcggaag
    13081 cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa gcccatcgcc
    13141 gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc attgatcgac
    13201 cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc gataggggtg
    13261 cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc ccgcagctcg
    13321 acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt gttttgcagc
    13381 gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc cgtgtcgttt
    13441 ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag ctgcatttcc
    13501 ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct gacctgtttt
    13561 gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg cgtgtcgatg
    13621 gtcatcgact tcgccaaacc tgccgcctcc tgttcgagac gacgcgaacg ctccacggcg
    13681 gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc gatcttggcc
    13741 gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc acgcatgacg
    13801 gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat cagttcttgc
    13861 ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat tgccccgact
    13921 cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt ggtgtccaga
    13981 taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc cttctcgtac
    14041 ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa atacttgccg
    14101 tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg ctcctgcttg
    14161 tcgccggcat cgttgcgcca catctaggta ctaaaacaat tcatccagta aaatataata
    14221 ttttattttc tcccaatcag gcttgatccc cagtaagtca aaaaatagct cgacatactg
    14281 ttcttccccg atatcctccc tgatcgaccg gacgcagaag gcaatgtcat accacttgtc
    14341 cgccctgccg cttctcccaa gatcaataaa gccacttact ttgccatctt tcacaaagat
    14401 gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc tcttcgggct tttccgtctt
    14461 taaaaaatca tacagctcgc gcggatcttt aaatggagtg tcttcttccc agttttcgca
    14521 atccacatcg gccagatcgt tattcagtaa gtaatccaat tcggctaagc ggctgtctaa
    14581 gctattcgta tagggacaat ccgatatgtc gatggagtga aagagcctga tgcactccgc
    14641 atacagctcg ataatctttt cagggctttg ttcatcttca tactcttccg agcaaaggac
    14701 gccatcggcc tcactcatga gcagattgct ccagccatca tgccgttcaa agtgcaggac
    14761 ctttggaaca ggcagctttc cttccagcca tagcatcatg tccttttccc gttccacatc
    14821 ataggtggtc cctttatacc ggctgtccgt catttttaaa tataggtttt cattttctcc
    14881 caccagctta tataccttag caggagacat tccttccgta tcttttacgc agcggtattt
    14941 ttcgatcagt tttttcaatt ccggtgatat tctcatttta gccatttatt atttccttcc
    15001 tcttttctac agtatttaaa gataccccaa gaagctaatt ataacaagac gaactccaat
    15061 tcactgttcc ttgcattcta aaaccttaaa taccagaaaa cagctttttc aaagttgttt
    15121 tcaaagttgg cgtataacat agtatcgacg gagccgattt tgaaaccaca attatgggtg
    15181 atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc agtggcttct
    15241 gtgtctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa cggcaaaagc
    15301 accgccggac atcagcgcta tctctgctct cactgccgta aaacatggca actgcagttc
    15361 acttacaccg cttctcaacc cggtacgcac cagaaaatca ttgatatggc catgaatggc
    15421 gttggatgcc gggcaacagc ccgcattatg ggcgttggcc tcaacacgat tttacgtcac
    15481 ttaaaaaact caggccgcag tcggtaacct cgcgcataca gccgggcagt gacgtcatcg
    15541 tctgcgcgga aatggacgaa cagtggggct atgtcggggc taaatcgcgc cagcgctggc
    15601 tgttttacgc gtatgacagt ctccggaaga cggttgttgc gcacgtattc ggtgaacgca
    15661 ctatggcgac gctggggcgt cttatgagcc tgctgtcacc ctttgacgtg gtgatatgga
    15721 tgacggatgg ctggccgctg tatgaatccc gcctgaaggg aaagctgcac gtaatcagca
    15781 agcgatatac gcagcgaatt gagcggcata acctgaatct gaggcagcac ctggcacggc
    15841 tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct gcatgacaaa gtcatcgggc
    15901 attatctgaa cataaaacac tatcaataag ttggagtcat tacccaatta tgatagaatt
    15961 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
    16021 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
    16081 tacatacggc gatatcttct atataaaaga tatattatct tatcagtatt gtcaatatat
    16141 tcaaggcaat ctgcctcctc atcctcttca tcctcttcgt cttggtagct ttttaaatat
    16201 ggcgcttcat agagtaattc tgtaaaggtc caattctcgt tttcatacct cggtataatc
    16261 ttacctatca cctcaaatgg ttcgctgggt ttatcgcacc cccgaacacg agcacggcac
    16321 ccgcgaccac tatgccaaga atgcccaagg taaaaattgc cggccccgcc atgaagtccg
    16381 tgaatgcccc gacggccgaa gtgaagggca ggccgccacc caggccgccg ccctcactgc
    16441 ccggcacctg gtcgctgaat gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg
    16501 tcgcgctcgg gctgatcgcc catcccgtta ctgccccgat cccggcaatg gcaaggactg
    16561 ccagcgctgc catttttggg gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg
    16621 atgggaggcc cgcgttagcg ggccgggagg gttcgagaag ggggggcacc ccccttcggc
    16681 gtgcgcggtc acgcgcacag ggcgcagccc tggttaaaaa caaggtttat aaatattggt
    16741 ttaaaagcag gttaaaagac aggttagcgg tggccgaaaa acgggcggaa acccttgcaa
    16801 atgctggatt ttctgcctgt ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg
    16861 tcagcactct gcccctcaag tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc
    16921 ctcaagtgtc aataccgcag ggcacttatc cccaggcttg tccacatcat ctgtgggaaa
    16981 ctcgcgtaaa atcaggcgtt ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc
    17041 cgaaatcgag cctgcccctc atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg
    17101 tcaacgtccg cccctcatct gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc
    17161 cggcggccgc ggtgtctcgc acacggcttc gacggcgttt ctggcgcgtt tgcagggcca
    17221 tagacggccg ccagcccagc ggcgagggca accagcccgg
//

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