pLKO-DEST-EGFP

价格:1250元

联系方式:I47-825O-882O

买家导航

载体基本信息

出品公司: Addgene
载体名称: pLKO-DEST-EGFP
质粒类型: 慢病毒载体;RNAi载体;cDNA/shRNA gateway
高拷贝/低拷贝: 低拷贝
克隆方法: gateway
启动子: U6
载体大小: 8856 bp
5' 测序引物及序列: LKO.1 5' :GACTATCATATGCTTACCGT
3' 测序引物及序列: --
载体标签: --
载体抗性: 氨苄青霉素、氯霉素
筛选标记: Hygromycin
克隆菌株: 阳性筛选用DB3.1 ,阴性筛选用OmniMAX2-T1, 重组慢病毒载体的复制用Stbl3感受态细胞,以维持质粒的稳定性。
宿主细胞(系): 哺乳动物细胞
备注:
其它筛选标记 pLKO-DEST-puro
                        pLKO-DEST-YFP
                        pLKO-DEST-hygro
 
产品目录号: # 32684
稳定性: 稳表达
组成型/诱导型: 组成型
病毒/非病毒: 慢病毒

载体质粒图谱和多克隆位点信息

pLKO-DEST-EGFP载体图谱



载体简介

载体序列

LOCUS       pLKO-DEST-EGFP	8856 bp 	DNA	SYN
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="pLKO.DEST.EGFP"
                     /mol_type="other DNA"
     misc_feature    complement(114..221)
                     /label="attR1"
     misc_feature    114..214
                     /label="attR2"
     CDS             241..747
                     /label="ORF frame 1"
                     /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD
                     IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP
                     RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA
                     PQLASCRT*"
     misc_feature    322..337
                     /label="cPPT"
                     /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD
                     IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP
                     RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA
                     PQLASCRT*"
     misc_feature    complement(559..582)
                     /label="MSCV_rev_primer"
                     /translation="MGGRSRGPKLTREFSTSRQMAVFIHNFKRKGGIGGYSAGERIVD
                     IIATDIQTKELQKQITKIQNFRVYYRDSRDPLWPRLEGVGVAPFPRLEFYRVGEALFP
                     RQSGACALAAPLGTWRYTSGLWPRTHSTSTGRRQPAPFFGGPFAPPSTPPLVRKFPPA
                     PQLASCRT*"
     CDS             804..1928
                     /label="ORF frame 3"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLAYHDRVQAHGAPRHPRRRPQGRT
                     HPRRRVRRLPRHAPHRRSGPNHNHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGR
                     GRCHLRQADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRL
                     RPGAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQR
                     LYHGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPE
                     QRPQREARSHGPAGVRDRRRDHSRHGRAVQVKRPHMHRYL*"
     misc_feature    956..975
                     /label="mPGK_F_primer"
                     /translation="MEAGRPLGQRPIAALLLRFLGSEAGKGWVRGRAQGRAQGRGGRP
                     KVLRRPGILHASKAHVCRAVLLFLISGPFDLQPKLAYHDRVQAHGAPRHPRRRPQGRT
                     HPRRRVRRLPRHAPHRRSGPNHNHGEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGR
                     GRCHLRQADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRL
                     RPGAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQR
                     LYHGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPE
                     QRPQREARSHGPAGVRDRRRDHSRHGRAVQVKRPHMHRYL*"
     CDS             1048..1899
                     /label="ORF frame 1"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     gene            1183..1896
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    complement(1225..1246)
                     /label="EGFP_N_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    1363..1392
                     /label="Y66 (EGFP)"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    1833..1854
                     /label="EGFP_C_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    1972..1987
                     /label="cPPT"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    1972..1993
                     /label="U3PPT"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    1989..2041
                     /label="delta_U3"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    2042..2222
                     /label="HIV-1_5_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    2042..2222
                     /label="truncHIV-1_3_LTR"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     terminator      2297..2416
                     /label="SV40_PA_terminator"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    2385..2404
                     /label="EBV_rev_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     rep_origin      2434..2532
                     /label="SV40_origin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     misc_feature    2517..2536
                     /label="SV40pro_F_primer"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     rep_origin      2748..3054
                     /label="f1_origin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     promoter        3247..3275
                     /label="AmpR_promoter"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     gene            3317..4177
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRTTT
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK*"
     CDS             3317..4177
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      4297..4881
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5192..5210
                     /label="M13_reverse_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        5227..5246
                     /label="T3_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5500..5680
                     /label="HIV-1_5_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5500..5680
                     /label="truncHIV-1_3_LTR"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    5791..5835
                     /label="HIV-1_psi_pack"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     CDS             6223..7080
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    6345..6578
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     promoter        7114..7346
                     /label="hU6_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    7276..7295
                     /label="LKO1_5_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    7390..7514
                     /label="attR1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     misc_feature    complement(7414..7514)
                     /label="attR2"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     promoter        7539..7562
                     /label="lac_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     gene            7623..8282
                     /label="CAT/CamR"
                     /gene="CAT/CamR"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYRSRD*"
     CDS             7623..8282
                     /label="ORF frame 3"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
     misc_feature    8624..73
                     /label="ccdB"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A*"
ORIGIN
    1 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
   61 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gaccatagtg
  121 actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa atctaattta
  181 atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac aaagtggttg
  241 atgggcggcc gctctagagg gcccaagctt acgCGTGAAT TCTCGACCTC GAGACAAATG
  301 GCAGTATTCA TCCACAATTT TAAAAGAAAA GGGGGGATTG GGGGGTACAG TGCAGGGGAA
  361 AGAATAGTAG ACATAATAGC AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA
  421 AAAATTCAAA ATTTTCGGGT TTATTACAGG GACAGCAGAG ATCCACTTTG GCCGCGGCTC
  481 GAGGGGGTTG GGGTTGCGCC TTTTCCAAGG CTAGAATTCT ACCGGGTAGG GGaggcgctt
  541 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
  601 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
  661 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
  721 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
  781 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
  841 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
  901 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
  961 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
 1021 tcgacctgca gcccaagcta gcttaccatg accgagtaca agcccacggt gcgcctcgcc
 1081 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
 1141 gccaCGCGcC ACACcGTCGA TCCGGACCGA ACCaCAacca tggtgagcaa gggcgaggag
 1201 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
 1261 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
 1321 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
 1381 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
 1441 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
 1501 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
 1561 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
 1621 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
 1681 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
 1741 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
 1801 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
 1861 gccgggatca ctctcggcat ggacgagctg tacaagtaaa gcggccgcat atgcatcggt
 1921 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
 1981 ggggggactg gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac
 2041 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
 2101 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
 2161 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
 2221 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
 2281 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
 2341 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
 2401 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
 2461 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
 2521 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
 2581 tttggaggcc tagggacgta cccaattcgc ctggcgttac ccaacttaat cgccttgcag
 2641 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
 2701 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
 2761 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
 2821 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
 2881 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
 2941 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
 3001 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
 3061 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
 3121 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
 3181 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
 3241 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
 3301 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
 3361 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
 3421 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
 3481 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
 3541 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
 3601 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
 3661 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
 3721 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
 3781 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
 3841 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
 3901 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
 3961 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
 4021 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
 4081 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
 4141 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
 4201 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
 4261 ttttgataat ctcatgagga agcggaagag cgctgcgcgt aatctgctgc ttgcaaacaa
 4321 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
 4381 cgaaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
 4441 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
 4501 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
 4561 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
 4621 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
 4681 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
 4741 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
 4801 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
 4861 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
 4921 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
 4981 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
 5041 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
 5101 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg
 5161 agttagctca ctcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
 5221 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct
 5281 tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa
 5341 ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta
 5401 ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc
 5461 agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag
 5521 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
 5581 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
 5641 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
 5701 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
 5761 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
 5821 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
 5881 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
 5941 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
 6001 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
 6061 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
 6121 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
 6181 cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga
 6241 agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag
 6301 gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt
 6361 gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag
 6421 gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag
 6481 gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc
 6541 ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga
 6601 aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa
 6661 cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
 6721 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
 6781 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
 6841 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
 6901 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
 6961 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
 7021 gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc acgagactag
 7081 cctcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc atatttgcat
 7141 atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa cacaaagata
 7201 ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc agttttaaaa
 7261 ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt cgatttcttg
 7321 gctttatata tcttgtggaa aggacgaaac accggTCCGC AGGTATGCAC GCGatcctcg
 7381 agcccatcaa caagtttgta caaaaaagct gaacgagaaa cgtaaaatga tataaatatc
 7441 aatatattaa attagatttt gcataaaaaa cagactacat aatactgtaa aacacaacat
 7501 atccagtcac tatggcggcc gcattaggca ccccaggctt tacactttat gcttccggct
 7561 cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct aaggaagcta
 7621 aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag
 7681 aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg
 7741 atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta
 7801 ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg
 7861 gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg
 7921 aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat
 7981 attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg
 8041 agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg
 8101 tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag
 8161 gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt gatggcttcc
 8221 atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag ggcggggcgt
 8281 aaagatctgg atccggctta ctaaaagcca gataacagta tgcgtatttg cgcgctgatt
 8341 tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa agaggtgtgc
 8401 tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc tcaaggcata
 8461 tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg
 8521 cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt
 8581 gaaatgaacg gctcttttgc tgacgagaac agggactggt gaaatgcagt ttaaggttta
 8641 cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg atattattga
 8701 cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt cagataaagt
 8761 ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca tgatgaccac
 8821 cgatatggcc agtgtgccgg tctccgttat cgggga
//

载体质粒图谱pdf版和相关资料下载

载体应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng