| 质粒名称: | pEYK3.1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 17366 |
| 存储实验室: | George Daley |
|
载体骨架基本信息 |
|
| 载体名称: | pEYK3.1 |
| 载体出品公司: | -- |
| 载体抗性: | 博来霉素 |
| 空载体大小: | 3422 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 逆转录病毒 |
| 筛选标记: | 博来霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | GFP |
| 物种来源: | -- |
| 插入大小: | 719 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEYK3.1 was a gift from George Daley (Addgene plasmid # 17366 ; http://n2t.net/addgene:17366 ; RRID:Addgene_17366) |
| 参考文献部分: | Novel retroviral vectors to facilitate expression screens in mammalian cells. Koh EY, Chen T, Daley GQ. Nucleic Acids Res. 2002 Dec 15. 30(24):e142. 10.1093/nar/gnf142 PubMed 12490733 |
买家导航
pEYK3.1载体质粒基本信息
pEYK3.1质粒图谱载体图谱和pEYK3.1载体序列质粒序列多克隆位点信息
pEYK3.1质粒载体简介
pEYK3.1质粒序列载体序列
LOCUS pEYK3.1 4139 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pEYK3.1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4139)
AUTHORS Koh EY, Chen T, Daley GQ
TITLE Novel retroviral vectors to facilitate expression screens in
mammalian cells.
JOURNAL Nucleic Acids Res. 2002 Dec 15. 30(24):e142.
PUBMED 12490733
REFERENCE 2 (bases 1 to 4139)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4139
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..470
/note="long terminal repeat from Moloney murine leukemia
virus"
protein_bind complement(579..612)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(858..1446)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(938..957)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1479..1853)
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
promoter complement(1872..1919)
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS complement(2088..2807)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(2133..2154)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 2482..2501
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2741..2762
/label=EGFP-N
/note="EGFP, reverse primer"
regulatory 2804..2813
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 2853..3227
/label=pol region
/note="Moloney murine leukemia virus (MMLV) pol region
containing the splice acceptor site"
primer_bind complement(2899..2915)
/label=pBMN 5'
/note="MMLV sequence, for inserts in pBMN retroviral
vector"
CDS complement(3237..3653)
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind complement(3253..3269)
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
primer_bind complement(3285..3307)
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
misc_feature 3718..4075
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
ORIGIN
1 tgaaagaccc ccgctgacgg gtagtcaatc actcagagga gaccctccca aggaacagcg
61 agaccacaag tcggatgcaa ctgcaagagg gtttattgga tacacgggta cccgggcgac
121 tcagtcaatc ggaggactgg cgccccgagt gaggggttgt gggctctttt attgagctcg
181 gggagcagaa gcgcgcgaac agaagcgaga agcgaactga ttggttagtt caaataaggc
241 acagggtcat ttcaggtcct tggggcaccc tggaaacatc tgatggttct ctagaaactg
301 ctgagggctg gaccgcatct ggggaccatc tgttcttggc cctgagccgg ggcaggaact
361 gcttaccaca gatatcctgt ttggcccata ttcagctgtt ccatctgttc ttggccctga
421 gccggggcag gaactgctta ccacagatat cctgtttggc ccatattcag ctgttccatc
481 tgttcctgac cttgatctga acttctctat tctcagttat gtatttttcc atgccttgca
541 aaatggcgtt acttaagcta gaggcgcgcc ttaattaaat aacttcgtat aatgtatgct
601 atacgaagtt atgcggccgc gctagcttgc caaacctaca ggtggggtct ttcattcccc
661 cctttttctg gagactaaat aaaatctttt attttatcta tggctcgtac tctataggct
721 tcagctggtg atattgttga gtcaaaacta gagcctggac cactgatatc ctgtctttaa
781 caaattggac taatcgatat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
1021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
1081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
1141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
1201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
1261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
1321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
1381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
1441 tctcaagaag atcctttgat cttttctacg ggacgcgttc agtcctgctc ctcggccacg
1501 aagtgcacgc agttgccggc cgggtcgcgc agggcgaact cccgccccca cggctgctcg
1561 ccgatctcgg tcatggccgg cccggaggcg tcccggaagt tcgtggacac gacctccgac
1621 cactcggcgt acagctcgtc caggccgcgc acccacaccc aggccagggt gttgtccggc
1681 accacctggt cctggaccgc gctgatgaac agagtcacgt cgtcccggac cacaccggcg
1741 aagtcgtcct ccacgaagtc ccgggagaac ccgagccggt cggtccagaa ctcgaccgct
1801 ccggcgacgt cgcgcgcggt gagcaccgga acggcactgg tcaacttggc catggtttag
1861 ttcctcacct tgtcgtatta tactatgccg atatactatg ccgatgatta attgtcaaca
1921 gtcgaccact gtgctggcgg ccggccgctc gagtttaaat agtacataat ggatttcctt
1981 acgcgaaata cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac
2041 tggtaatggt agcgaccggc gctcagctgg aattcttaat taattcatta cttgtacagc
2101 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
2161 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
2221 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
2281 ctgccgtcct cgatgttgtg gcggatcttg aagttcgcct tgatgccgtt cttctgcttg
2341 tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg
2401 ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc
2461 tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc
2521 tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg
2581 gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc
2641 acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg
2701 ccctcgccct cgccagacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc
2761 aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgttaatt
2821 aagaattccc gtaccaccac actgggatcc ggcagtctag aggatggtcc acccccgggg
2881 tcggcagcct tcacgtgggc ggcgtgtatc caagctgcga tgccgtctac tttgagggcg
2941 gtgggggtgg tcagcaggac tgtgtaaggt cctttccagc gaggttctag gttcttagtc
3001 tggtgtcggc ggacccacac tgtgtcgccg actcggtaag ggtgaggtac caccggtcgg
3061 tccagttgtt cttggtaggc tgccgccaga ggtctccaga cttcgtgctg gactaagtag
3121 agagcctgta agtgagcttg gagagagggg ctgttagtaa ctcttgtctt gtcagggtca
3181 gggaagttta caaggggcgg gggtgcccca tataagatct catatggcca tatgggggcg
3241 cctagagaag gagtgagggc tggataaagg gaggatcgag gcggggtcga acgaggaggt
3301 tcaaggggga gagacggggc ggatggagga agaggaggcg gaggcttagg gtgtacaaag
3361 ggcttgaccc agggaggggg gtcaaaagcc aaggcttccc aggtcacgat gtaggggacc
3421 tggtctgggt gtccatgcgg gccaggtgaa aagaccttga tcttaacctg ggtgatgagg
3481 tctcggttaa aggtgccgtc tcgcggccat ccgacgttaa aggttggcca ttctgcagag
3541 cagaaggtaa cccaacgtct cttcttgaca tctaccgact ggttgtgagc gatccgctcg
3601 acatctttcc agtgacctaa ggtcaaactt aagggagtgg taacagtctg gcccgggccc
3661 ttattttcag acaaatacag aaacacagtc agacagagac aacacagaac gatgctgcag
3721 cagacaagac gcgcggcgcg gcttcggtcc caaaccgaaa gcaaaaattc agacggaggc
3781 gggaactgtt ttaggttctc gtctcctacc agaaccacat atccctcctc taaggggggt
3841 gcaccaaaga gtccaaaacg atcgggattt ttggactcag gtcgggccac aaaaacggcc
3901 cccgaagtcc ctgggacgtc tcccagggtt gcggccgggt gttccgaact cgtcagttcc
3961 accacgggtc cgccagatac agagctagtt agctaactag taccgacgca ggcgcataaa
4021 atcagtcata gacactagac aatcggacag acacagataa gttgctggcc agcttacctc
4081 ccggtggtgg gtcggtggtc cctgggcagg ggtctcccga tcccggacga gcccccaaa
//
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