ML851 pSP6-nsP1-nsP3 (with ALI mutations)-GFP-MEK1-P2A-PuroR

LOCUS      ML851 pSP6-nsP1-nsP3 (with ALI mutations)-GFP-MEK1-P2A-PuroR              11148 bp DNA     circular SYN 18-OCT-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    ML851 pSP6-nsP1-nsP3 (with ALI mutations)-GFP-MEK1-P2A-PuroR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11148)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Jul 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11148
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          6975..8153
                     /chromosome="15"
                     /map="15q22.31"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /organism="Homo sapiens"
     CDS             60..1679
                     /codon_start=1
                     /label=nsP1
                     /note="nsP1"
                     /translation="MEKPVVNVDVDPQSPFVVQLQKSFPQFEVVAQQVTPNDHANARAF
                     SHLASKLIELEVPTTATILDIGSAPARRMFSEHQYHCVCPMRSPEDPDRMMKYASKLAE
                     KACKITNKNLHEKIKDLRTVLDTPDAETPSLCFHNDVTCNTRAEYSVMQDVYINAPGTI
                     YHQAMKGVRTLYWIGFDTTQFMFSAMAGSYPAYNTNWADEKVLEARNIGLCSTKLSEGR
                     TGKLSIMRKKELKPGSRVYFSVGSTLYPEHRASLQSWHLPSVFHLKGKQSYTCRCDTVV
                     SCEGYVVKKITISPGITGETVGYAVTNNSEGFLLCKVTDTVKGERVSFPVCTYIPATIC
                     DQMTGIMATDISPDDAQKLLVGLNQRIVINGKTNRNTNTMQNYLLPIIAQGFSKWAKER
                     KEDLDNEKMLGTRERKLTYGCLWAFRTKKVHSFYRPPGTQTIVKVPASFSAFPMSSVWT
                     TSLPMSLRQKIKLALQPKKEEKLLQVPEELVMEAKAAFEDAQEESRAEKLREALPPLVA
                     DKGIEAAAEVVCEVEGLQADIAA"
     misc_feature    1674..1676
                     /label=G539A
                     /note="G539A"
     CDS             1680..4100
                     /codon_start=1
                     /label=nsP2
                     /note="nsP2"
                     /translation="ALVETPRGHVRIIPQANDRMIGQYIVVSPTSVLKNAKLAPAHPLA
                     DQVKIITHSGRSGRYAVEPYDAKVLMPAGSAVPWPEFLALSESATLVYNEREFVNRKLY
                     HIAMHGPAKNTEEEQYKVTKAELAETEYVFDVDKKRCVKKEEASGLVLSGELTNPPYHE
                     LALEGLKTRPAVPYKVETIGVIGTPGSGKSAIIKSTVTARDLVTSGKKENCREIEADVL
                     RLRGMQITSKTVDSVMLNGCHKAVEVLYVDEAFACHAGALLALIAIVRPRKKVVLCGDP
                     KQCGFFNMMQLKVYFNHPEKDICTKTFYKFISRRCTQPVTAIVSTLHYDGKMKTTNPCK
                     KNIEIDITGATKPKPGDIILTCFRGWVKQLQIDYPGHEVMTAAASQGLTRKGVYAVRQK
                     VNENPLYAITSEHVNVLLTRTEDRLVWKTLQGDPWIKQLTNVPKGNFQATIEDWEAEHK
                     GIIAAINSPAPRTNPFSCKTNVCWAKALEPILATAGIVLTGCQWSELFPQFADDKPHSA
                     IYALDVICIKFFGMDLTSGLFSKQSIPLTYHPADSARPVAHWDNSPGTRKYGYDHAVAA
                     ELSRRFPVFQLAGKGTQLDLQTGRTRVISAQHNLVPVNRNLPHALVPEHKEKQPGPVKK
                     FLSQFKHHSVLVVSEEKIEAPHKRIEWIAPIGIAGADKNYNLAFGFPPQARYDLVFINI
                     GTKYRNHHFQQCEDHAATLKTLSRSALNCLNLGGTLVVKSYGYADRNSEDVVTALARKF
                     VRVSAARPECVSSNTEMYLIFRQLDNSRTRQFTPHHLNCVISSVYEGTRDGVIA"
     exon            3855..3857
                     /note="P726L"
     misc_feature    4095..4097
                     /label=G806I
                     /note="G806I"
     CDS             4101..5762
                     /codon_start=1
                     /label=nsP3
                     /note="nsP3"
                     /translation="APSYRTKRENIADCQEEAVVNAANPLGRPGEGVCRAIYKRWPNSF
                     TDSATETGTAKLTVCQGKKVIHAVGPDFRKHPEAEALKLLQNAYHAVADLVNEHNIKSV
                     AIPLLSTGIYAAGKDRLEVSLNCLTTALDRTDADVTIYCLDKKWKERIDAVLQLKESVT
                     ELKDEDMEIDDELVWIHPDSCLKGRKGFSTTKGKLYSYFEGTKFHQAAKDMAEIKVLFP
                     NDQESNEQLCAYILGETMEAIREKCPVDHNPSSSPPKTLPCLCMYAMTPERVHRLRSNN
                     VKEVTVCSSTPLPKYKIKNVQKVQCTKVVLFNPHTPAFVPARKYIEAPEQPAAPPAQAE
                     EAPEVAATPTPPAADNTSLDVTDISLDMEDSSEGSLFSSFSGSDNSITSMDSWSSGPSS
                     LEIVDRRQVVVADVHAVQEPAPVPPPRLKKMARLAAARMQEEPTPPASTSSADESLHLS
                     FGGVSMSFGSLFDGEMARLAAAQPPASTCPTDVPMSFGSFSDGEIEELSRRVTESEPVL
                     FGSFEPGEVNSIISSRSAVSFPPRKQRRRRRSRRTEY"
     regulatory      5912..5921
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5918..6634
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=enhanced GFP
                     /note="EGFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             6975..8153
                     /codon_start=1
                     /gene="MAP2K1"
                     /gene_synonym="CFC3; MAPKK1; MEK1; MKK1; PRKMK1"
                     /product="dual specificity mitogen-activated protein kinase
                     kinase 1"
                     /EC_number="2.7.12.2"
                     /label=MAP2K1
                     /note="MEK1"
                     /note="protein kinase, mitogen-activated, kinase 1 (MAP
                     kinase kinase 1); MEK 1; MAPKK 1; MAPK/ERK kinase 1; ERK 
                     activator kinase 1"
                     /db_xref="GI:5579478"
                     /db_xref="CCDS:CCDS10216.1"
                     /db_xref="GeneID:5604"
                     /db_xref="HGNC:HGNC:6840"
                     /db_xref="MIM:176872"
                     /protein_id="NP_002746.1"
                     /translation="MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQ
                     QRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIK
                     PAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPE
                     QILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANS
                     FVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEG
                     DAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNK
                     CLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV"
     CDS             8154..8210
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=2A peptide from porcine teschovirus-1
                     polyprotein
                     /note="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8211..8810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             8211..8807
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=pac from Streptomyces alboniger
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    9169..9252
                     /label=antigenomic ribozyme HDV
                     /note="antigenomic ribozyme HDV"
     promoter        9319..9423
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             9424..10284
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      10455..11043
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        join(11131..11148,1)
                     /note="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 attggcggcg tagtacacac tattgaatca aacagccgac caattgcact accatcacaa
       61 tggagaagcc agtagttaac gtagacgtag acccgcagag tccgtttgtc gtgcaactgc
      121 aaaagagctt cccgcaattt gaggtagtag cacagcaggt cactccaaat gaccatgcta
      181 atgccagagc attttcgcat ctggccagta aactaatcga gctggaggtt cctaccacag
      241 cgacgatttt ggacataggc agcgcaccgg ctcgtagaat gttttccgag caccagtacc
      301 attgcgtttg ccccatgcgt agtccagaag acccggaccg catgatgaaa tatgccagca
      361 aactggcgga aaaagcatgc aagattacga ataagaactt gcatgagaag atcaaggacc
      421 tccggaccgt acttgataca ccggatgctg aaacgccatc actctgcttc cacaacgatg
      481 ttacctgcaa cacgcgtgcc gagtactccg tcatgcagga cgtgtacatc aacgcacccg
      541 gaactattta ccatcaggct atgaaaggcg tgcggaccct gtactggatt ggcttcgata
      601 ccacccagtt catgttctcg gctatggcag gttcgtaccc tgcgtacaac accaactggg
      661 ccgacgaaaa agtcctcgaa gcgcgtaaca tcggactctg cagcacaaag ctgagtgaag
      721 gcaggacagg aaagttgtcg ataatgagga agaaggagtt gaagcccggg tcacgggttt
      781 atttctccgt tggatcgaca ctttacccag aacacagagc cagcttgcag agctggcatc
      841 ttccatcggt gttccacctg aaaggaaagc agtcgtacac ttgccgctgt gatacagtgg
      901 tgagctgcga aggctacgta gtgaagaaaa tcaccatcag tcccgggatc acgggagaaa
      961 ccgtgggata cgcggttaca aacaatagcg agggcttctt gctatgcaaa gttaccgata
     1021 cagtaaaagg agaacgggta tcgttccccg tgtgcacgta tatcccggcc accatatgcg
     1081 atcagatgac cggcataatg gccacggata tctcacctga cgatgcacaa aaacttctgg
     1141 ttgggctcaa ccagcgaatc gtcattaacg gtaagactaa caggaacacc aataccatgc
     1201 aaaattacct tctgccaatc attgcacaag ggttcagcaa atgggccaag gagcgcaaag
     1261 aagaccttga caatgaaaaa atgctgggta ccagagagcg caagcttaca tatggctgct
     1321 tgtgggcgtt tcgcactaag aaagtgcact cgttctatcg cccacctgga acgcagacca
     1381 tcgtaaaagt cccagcctct tttagcgctt tccccatgtc atccgtatgg actacctctt
     1441 tgcccatgtc gctgaggcag aagataaaat tggcattaca accaaagaag gaggaaaaac
     1501 tgctgcaagt cccggaggaa ttagtcatgg aggccaaggc tgctttcgag gatgctcagg
     1561 aggaatccag agcggagaag ctccgagaag cactcccacc attagtggca gacaaaggta
     1621 tcgaggcagc cgcggaagtt gtctgcgaag tggaggggct ccaggcggac atcgctgcag
     1681 cactcgtcga aaccccgcgc ggtcatgtaa ggataatacc tcaagcaaat gaccgtatga
     1741 tcggacagta catcgttgtc tcgccaacct ctgtgctgaa gaacgctaaa ctcgcaccag
     1801 cacacccgct agcagaccag gttaagatca taacgcactc cggaagatca ggaaggtatg
     1861 cagtcgaacc atacgacgct aaagtactga tgccagcagg aagtgccgta ccatggccag
     1921 aattcttagc actgagtgag agcgccacgc tagtgtacaa cgaaagagag tttgtgaacc
     1981 gcaagctgta ccatattgcc atgcacggtc ccgctaagaa tacagaagag gagcagtaca
     2041 aggttacaaa ggcagagctc gcagaaacag agtacgtgtt tgacgtggac aagaagcgat
     2101 gcgtcaagaa ggaagaagcc tcaggacttg tcctctcggg agaactgacc aacccgccct
     2161 atcacgaact agctcttgag ggactgaaga ctcgacccgc ggtcccgtac aaggttgaaa
     2221 caataggagt gataggcaca ccaggatcgg gcaagtcggc tatcatcaag tcaactgtca
     2281 cggcacgtga tcttgttacc agcggaaaga aagaaaactg ccgcgaaatt gaggccgatg
     2341 tgctacggct gaggggcatg cagatcacgt cgaagacagt ggattcggtt atgctcaacg
     2401 gatgccacaa agccgtagaa gtgctgtatg ttgacgaagc gttcgcgtgc cacgcaggag
     2461 cactacttgc cttgattgca atcgtcagac cccgtaagaa ggtagtgcta tgcggagacc
     2521 ctaagcaatg cggattcttc aacatgatgc aactaaaggt atatttcaac cacccggaaa
     2581 aagacatatg taccaagaca ttctacaagt ttatctcccg acgttgcaca cagccagtca
     2641 cggctattgt atcgacactg cattacgatg gaaaaatgaa aaccacaaac ccgtgcaaga
     2701 agaacatcga aatcgacatt acaggggcca cgaagccgaa gccaggggac atcatcctga
     2761 catgcttccg cgggtgggtt aagcaactgc aaatcgacta tcccggacat gaggtaatga
     2821 cagccgcggc ctcacaaggg ctaaccagaa aaggagtata tgccgtccgg caaaaagtca
     2881 atgaaaaccc gctgtacgcg atcacatcag agcatgtgaa cgtgctgctc acccgcactg
     2941 aggacaggct agtatggaaa actttacagg gcgacccatg gattaagcag ctcactaacg
     3001 taccaaaagg aaattttcaa gccaccatcg aggactggga agctgaacac aagggaataa
     3061 ttgctgcgat aaacagtccc gctccccgta ccaatccgtt cagctgcaag actaacgttt
     3121 gctgggcgaa agcactggaa ccgatactgg ccacggccgg tatcgtactt accggttgcc
     3181 agtggagcga gctgttccca cagtttgcag atgacaaacc acactcggcc atctacgccc
     3241 tggacgtaat ctgcattaag tttttcggca tggacttgac aagcggactg ttttccaaac
     3301 agagcatccc gttaacgtac catcctgccg attcagcgag gccagtagct cattgggaca
     3361 acagcccagg aacccgcaag tatgggtacg atcacgccgt tgccgccgaa ctctcccgta
     3421 gatttccggt gttccagcta gctgggaaag gcacacagct tgatttgcag acgggcagaa
     3481 ctagagttat ctccgcacag cataacttgg tcccagtgaa ccgcaatctc ccgcacgcct
     3541 tagtccccga gcacaaggag aaacaacccg gcccggtcaa aaaattcttg agccagttca
     3601 aacaccactc cgtacttgtg gtctcagagg aaaaaattga agctccccac aagagaatcg
     3661 aatggatcgc cccgattggc atagccggcg ctgataagaa ctacaacctg gctttcgggt
     3721 ttccgccgca ggcacggtac gacctggtgt ttatcaatat tggaactaaa tacagaaacc
     3781 atcactttca gcagtgcgaa gaccatgcgg cgaccttgaa aaccctctcg cgttcggccc
     3841 tgaactgcct taaccttgga ggcaccctcg tggtgaagtc ctacggttac gccgaccgca
     3901 atagtgagga cgtagtcacc gctcttgcca gaaaatttgt cagagtgtct gcagcgaggc
     3961 cagagtgcgt ctcaagcaat acagaaatgt acctgatctt ccgacaacta gacaacagcc
     4021 gcacacgaca attcaccccg catcatctga attgtgtgat ttcgtccgtg tacgagggta
     4081 caagagacgg agttattgcc gcaccgtcat accgcactaa aagggagaac attgctgatt
     4141 gtcaagagga agcagttgtc aatgcagcca atccgctggg cagaccaggc gaaggagtct
     4201 gccgtgccat ctataaacgt tggccgaaca gtttcaccga ttcagccaca gagaccggca
     4261 ccgcaaaact gactgtgtgc caaggaaaga aagtgatcca cgcggttggc cctgatttcc
     4321 ggaaacaccc agaggcagaa gccctgaaat tgctgcaaaa cgcctaccat gcagtggcag
     4381 acttagtaaa tgaacataat atcaagtctg tcgccatccc actgctatct acaggcattt
     4441 acgcagccgg aaaagaccgc cttgaagtat cacttaactg cttgacaacc gcgctagata
     4501 gaactgatgc ggacgtaacc atctactgcc tggataagaa gtggaaggaa agaatcgacg
     4561 cggtgctcca acttaaggag tctgtaacag agctgaagga tgaggatatg gagatcgacg
     4621 acgagttagt atggatccat ccggacagtt gcctgaaggg aagaaaggga ttcagtacta
     4681 caaaaggaaa gttgtattcg tactttgaag gcaccaaatt ccatcaagca gcaaaagata
     4741 tggcggagat aaaggtcctg ttcccaaatg accaggaaag caacgagcaa ctgtgtgcct
     4801 acatattggg ggagaccatg gaagcaatcc gcgaaaaatg cccggtcgac cacaacccgt
     4861 cgtctagccc gccaaaaacg ctgccgtgcc tctgcatgta tgccatgacg ccagaaaggg
     4921 tccacagact cagaagcaac aacgtcaaag aagttacagt atgctcctcc accccccttc
     4981 caaagtacaa aatcaagaac gttcagaagg ttcagtgcac aaaagtagtc ctgtttaacc
     5041 cgcatacccc tgcattcgtt cccgcccgta agtacataga agcgccagaa cagcctgcag
     5101 ctccgcctgc acaggccgag gaggcccccg aagttgcagc aacaccaaca ccacctgcag
     5161 ctgataacac ctcgcttgat gtcacggaca tctcactgga catggaagac agtagcgaag
     5221 gctcactctt ttcgagcttt agcggatcgg acaactctat tactagtatg gacagttggt
     5281 cgtcaggacc tagttcacta gagatagtag accgaaggca ggtggtggtg gctgacgtcc
     5341 atgccgtcca agagcctgcc cctgttccac cgccaaggct aaagaagatg gcccgcctgg
     5401 cagcggcaag aatgcaggaa gagccaactc caccggcaag caccagctct gcggacgagt
     5461 cccttcacct ttcttttggt ggggtatcca tgtccttcgg atcccttttc gacggagaga
     5521 tggcccgctt ggcagcggca caacccccgg caagtacatg ccctacggat gtgcctatgt
     5581 ctttcggatc gttttccgac ggagagattg aggagctgag ccgcagagta accgagtctg
     5641 agcccgtcct gtttgggtca tttgaaccgg gcgaagtgaa ctcaattata tcgtcccgat
     5701 cagccgtatc ttttccacca cgcaagcaga gacgtagacg caggagcagg aggaccgaat
     5761 actaattaat taaaggtaga caacatcaca cctgtcctgc tggcattgag aacttttgcc
     5821 cagagcaaaa gagcatttca agccatcaga ggggaaataa agcatctcta cggtggtcct
     5881 aaatagtcag catagtacat ttcgcgatcg cgccaccatg gtgagcaagg gcgaggagct
     5941 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     6001 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     6061 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     6121 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     6181 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     6241 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     6301 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     6361 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     6421 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     6481 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     6541 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     6601 cgggatcact ctcggcatgg acgagctgta caagtaacct gcaggaaaag gctgtttaag
     6661 ttgggtaaac cgctcccagc cgacgatgag caagacgaag acagaagacg cgctctgcta
     6721 gatgaaacaa aggcgtggtt tagagtaggt ataacagaca ccttagcagt ggccgtggca
     6781 actcggtatg aggtagacaa catcacacct gtcctgctgg cattgagaac ttttgcccag
     6841 agcaaaagag catttcaagc catcagaggg gaaataaagc atctctacgg tggtcctaaa
     6901 tagtcagcat agtacatttc atctgactaa taccacaaca ccaccagcgg ccgcatcgat
     6961 caccggtcgc caccatgccc aagaagaagc cgacgcccat ccagctgaac ccggcccccg
     7021 acggctctgc agttaacggg accagctctg cggagaccaa cttggaggcc ttgcagaaga
     7081 agctggagga gctagagctt gatgagcagc agcgaaagcg ccttgaggcc tttcttaccc
     7141 agaagcagaa ggtgggagaa ctgaaggatg acgactttga gaagatcagt gagctggggg
     7201 ctggcaatgg cggtgtggtg ttcaaggtct cccacaagcc ttctggcctg gtcatggcca
     7261 gaaagctaat tcatctggag atcaaacccg caatccggaa ccagatcata agggagctgc
     7321 aggttctgca tgagtgcaac tctccgtaca tcgtgggctt ctatggtgcg ttctacagcg
     7381 atggcgagat cagtatctgc atggagcaca tggatggagg ttctctggat caagtcctga
     7441 agaaagctgg aagaattcct gaacaaattt taggaaaagt tagcattgct gtaataaaag
     7501 gcctgacata tctgagggag aagcacaaga tcatgcacag agatgtcaag ccctccaaca
     7561 tcctagtcaa ctcccgtggg gagatcaagc tctgtgactt tggggtcagc gggcagctca
     7621 tcgactccat ggccaactcc ttcgtgggca caaggtccta catgtcgcca gaaagactcc
     7681 aggggactca ttactctgtg cagtcagaca tctggagcat gggactgtct ctggtagaga
     7741 tggcggttgg gaggtatccc atccctcctc cagatgccaa ggagctggag ctgatgtttg
     7801 ggtgccaggt ggaaggagat gcggctgaga ccccacccag gccaaggacc cccgggaggc
     7861 cccttagctc atacggaatg gacagccgac ctcccatggc aatttttgag ttgttggatt
     7921 acatagtcaa cgagcctcct ccaaaactgc ccagtggagt gttcagtctg gaatttcaag
     7981 attttgtgaa taaatgctta ataaaaaacc ccgcagagag agcagatttg aagcaactca
     8041 tggttcatgc ttttatcaag agatctgatg ctgaggaagt ggattttgca ggttggctct
     8101 gctccaccat cggccttaac cagcccagca caccaaccca tgctgctggc gtcgcaacaa
     8161 acttctcact actcaaacaa gcaggtgacg tggaggagaa tcccgggcct atgaccgagt
     8221 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8281 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8341 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8401 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8461 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8521 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8581 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8641 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8701 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8761 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctag ccaggagtgg
     8821 catcgatact agtacgcccc aatgacccga ccagcaaaac tcgatgtact tccgaggaac
     8881 tgatgtgcat aatgcatcag gctggtatat tagatccccg cttaccgcgg gcaatatagc
     8941 aacaccaaaa ctcgacgtat ttccgaggag gcgcagtgca taatgctgcg cagtgttgcc
     9001 aaataatcac tatattaacc atttattcag cggacgccaa aactcaatgt atttctgagg
     9061 aagcatggtg cataatgcca tgcagcgtct gcataacttt ttattatttc ttttattaat
     9121 caacaaaatt ttgtttttaa catttaaaaa aaaaaaaaaa aaaaaaaagg gtcggcatgg
     9181 catctccacc tcctcgcggt ccgacctggg catccgaagg aggacgcacg tccactcgga
     9241 tggctaaggg agggggatct cgaggggaat taattcttga agacgaaagg gccaggtggc
     9301 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     9361 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     9421 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     9481 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9541 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9601 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9661 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9721 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9781 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9841 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9901 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9961 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
    10021 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
    10081 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
    10141 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
    10201 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
    10261 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
    10321 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
    10381 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
    10441 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
    10501 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10561 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
    10621 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10681 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10741 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10801 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10861 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10921 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10981 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    11041 aaaaacgcca gcaacgcgag ctcgtatgga catattgtcg ttagaacgcg gctacaatta
    11101 atacataacc ttatgtatca tacacatacg atttaggtga cactatag
//