| 质粒名称: | p16L1L2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 45291 |
| 存储实验室: | John Schiller |
|
载体骨架基本信息 |
|
| 载体名称: | custom |
| 载体出品公司: | -- |
| 载体抗性: | 博莱霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | HPV16L1+L2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | EM7 |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p16L1L2 was a gift from John Schiller (Addgene plasmid # 45291 ; http://n2t.net/addgene:45291 ; RRID:Addgene_45291) |
| 参考文献部分: | Production of papillomavirus-based gene transfer vectors. Buck CB, Thompson CD. Curr Protoc Cell Biol. 2007 Dec;Chapter 26:Unit 26.1. doi: 10.1002/0471143030.cb2601s37. 10.1002/0471143030.cb2601s37 PubMed 18228512 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p16L1L2 6388 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS p16L1L2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6388)
AUTHORS Buck CB, Thompson CD
TITLE Production of papillomavirus-based gene transfer vectors.
JOURNAL Curr Protoc Cell Biol. 2007 Dec;Chapter 26:Unit 26.1. doi:
10.1002/0471143030.cb2601s37.
PUBMED 18228512
REFERENCE 2 (bases 1 to 6388)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6388
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(8..28)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 13..342
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 193..328
/label=SV40 ori
/note="SV40 origin of replication"
intron 415..511
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
polyA_signal 2065..2186
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2076..2095)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 2130..2149
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
promoter 2387..2598
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2611..2879
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
polyA_signal 4477..5049
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
rep_origin 5220..5808
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5709..5728
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 5894..5941
/label=EM7 promoter
/note="synthetic bacterial promoter "
promoter 5933..5951
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 cccctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
61 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
181 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
241 tgactaattt tttttattta tacagaggcc gaggccgcct cggcctctga gctattccag
301 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttga ttgggatcca
361 ccggactcta gaggatccgg tactcgagga actgaaaaac cagaaagtta actggtaagt
421 ttagtctttt tgtcttttat ttcaggtccc ggatccggtg gtggtgcaaa tcaaagaact
481 gctcctcagt ggatgttgcc tttacttcta ggcctgtacg gaagtgttac ttctgctcta
541 aaagctgcgg aattgtaccc gcggccgctc tagagccacc atgaggcaca agaggagcgc
601 caagaggacc aagagggcca gcgccaccca gctgtacaag acctgcaagc aggccggcac
661 ctgccccccc gacatcatcc ccaaggtgga gggcaagacc atcgccgacc agatcctgca
721 gtacggcagc atgggcgtgt tcttcggcgg cctgggcatc ggcaccggca gcggcaccgg
781 cggcaggacc ggctacatcc ccctgggcac caggcccccc accgccaccg acaccctggc
841 ccccgtgagg ccccccctga ccgtggaccc cgtgggcccc agcgacccca gcatcgtgag
901 cctggtggag gagaccagct tcatcgacgc cggcgccccc accagcgtgc ccagcatccc
961 ccccgacgtg agcggcttca gcatcaccac cagcaccgac accacccccg ccatcctgga
1021 catcaacaac accgtgacca ccgtgaccac ccacaacaac cccaccttca ccgaccccag
1081 cgtgctgcag ccccccaccc ccgccgagac cggcggccac ttcaccctga gcagcagcac
1141 catcagcacc cacaactacg aggagatccc catggacacc ttcatcgtga gcaccaaccc
1201 caacaccgtg accagcagca cccccatccc cggcagcagg cccgtggcca ggctgggcct
1261 gtacagcagg accacccagc aggtgaaggt ggtggacccc gccttcgtga ccacccccac
1321 caagctgatc acctacgaca accccgccta cgagggcatc gacgtggaca acaccctgta
1381 cttcagcagc aacgacaaca gcatcaacat cgcccccgac cccgacttcc tggacatcgt
1441 ggccctgcac aggcccgccc tgaccagcag gaggaccggc atcaggtaca gcaggatcgg
1501 caacaagcag accctgagga ccaggagcgg caagagcatc ggcgccaagg tgcactacta
1561 ctacgacctg agcaccatcg accccgccga ggagatcgag ctgcagacca tcacccccag
1621 cacctacacc accaccagcc acgccgccag ccccaccagc atcaacaacg gcctgtacga
1681 catctacgcc gacgacttca tcaccgacac cagcaccacc cccgtgccca gcgtgcccag
1741 caccagcctg agcggctaca tccccgccaa caccaccatc cccttcggtg gcgcctacaa
1801 catccccctg gtgagcggcc ccgacatccc catcaacatc accgaccagg cccccagcct
1861 gatccccatc gtgcccggca gcccccagta caccatcatc gccgacgccg gcgacttcta
1921 cctgcacccc agctactaca tgctgaggaa gaggaggaag aggctgccct acttcttcag
1981 cgacgtgagc ctggccgcct gaaagctttt tgaattcttt ggatccacta gtgtcgacta
2041 gagggccgct tcgagcagac atgataagat acattgatga gtttggacaa accacaacta
2101 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
2161 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
2221 ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa
2281 tcgataagga tccgggctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
2341 agttgcggtg gagaagagca tgcgtgaggc tccggtgccc gtcagtgggc agagcgcaca
2401 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
2461 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
2521 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
2581 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc
2641 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt
2701 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc
2761 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg
2821 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc
2881 aagctgtgac cggcccgctc tagagccacc atgagcctgt ggctgcccag cgaggccacc
2941 gtgtacctgc cccccgtgcc cgtgagcaag gtggtgagca ccgacgagta cgtggccagg
3001 accaacatct actaccacgc cggcaccagc aggctgctgg ccgtgggcca cccctacttc
3061 cccatcaaga agcccaacaa caacaagatc ctggtgccca aggtgagcgg cctgcagtac
3121 agggtgttca ggatccacct gcccgacccc aacaagttcg gcttccccga caccagcttc
3181 tacaaccccg acacccagag gctggtgtgg gcctgcgtgg gcgtggaggt gggcaggggc
3241 cagcccctgg gcgtgggcat cagcggccac cccctgctga acaagctgga cgacaccgag
3301 aacgccagcg cctacgccgc caacgccggc gtggacaaca gggagtgcat cagcatggac
3361 tacaagcaga cccagctgtg cctgatcggc tgcaagcccc ccatcggcga gcactggggc
3421 aagggcagcc cctgcaccaa cgtggccgtg aaccccggcg actgcccccc cctggagctg
3481 atcaacaccg tgatccagga cggcgacatg gtggacaccg gcttcggcgc catggacttc
3541 accaccctgc aggccaacaa gagcgaggtg cccctggaca tctgcaccag catctgcaag
3601 taccccgact acatcaagat ggtgagcgag ccctacggcg acagcctgtt cttctacctg
3661 aggagggagc agatgttcgt gaggcacctg ttcaacaggg ccggcgccgt gggcgagaac
3721 gtgcccgacg acctgtacat caagggcagc ggcagcaccg ccaacctggc cagcagcaac
3781 tacttcccca cccccagcgg cagcatggtg accagcgacg cccagatctt caacaagccc
3841 tactggctgc agagggccca gggccacaac aacggcatct gctggggcaa ccagctgttc
3901 gtgaccgtgg tggacaccac caggagcacc aacatgagcc tgtgcgccgc catcagcacc
3961 agcgagacca cctacaagaa caccaacttc aaggagtacc tgaggcacgg cgaggagtac
4021 gacctgcagt tcatcttcca gctgtgcaag atcaccctga ccgccgacgt gatgacctac
4081 atccacagca tgaacagcac catcctggag gactggaact tcggcctgca gccccccccc
4141 ggcggcaccc tggaggacac ctacaggttc gtgaccagcc aggccatcgc ctgccagaag
4201 cacacccccc ccgcccccaa ggaggacccc ctgaagaagt acaccttctg ggaggtgaac
4261 ctgaaggaga agttcagcgc cgacctggac cagttccccc tgggcaggaa gttcctgctg
4321 caggccggcc tgaaggccaa gcccaagttc accctgggca agaggaaggc cacccccacc
4381 accagcagca ccagcaccac cgccaagagg aagaagagga agctgtgaaa gctacccacg
4441 gccgaatagc cgtgagccgg aatcctgcac gctagcatta tccctaatac ctgccacccc
4501 actcttaatc agtggtggaa gaacggtctc agaactgttt gtttcaattg gccatttaag
4561 tttagtagta aaagactggt taatgataac aatgcatcgt aaaaccttca gaaggaaagg
4621 agaatgtttt gtggaccact ttggttttct tttttgcgtg tggcagtttt aagttattag
4681 tttttaaaat cagtactttt taatggaaac aacttgacca aaaatttgtc acagaatttt
4741 gagacccatt aaaaaagtta aatgagaaac ctgtgtgttc ctttggtcaa caccgagaca
4801 tttaggtgaa agacatctaa ttctggtttt acgaatctgg aaacttcttg aaaatgtaat
4861 tcttgagtta acacttctgg gtggagaata gggttgtttt ccccccacat aattggaagg
4921 ggaaggaata tcatttaaag ctatgggagg gtttctttga ttacaacact ggagagaaat
4981 gcagcatgtt gctgattgcc tgtcactaaa acaggccaaa aactgagtcc ttgggttgca
5041 tagaaagctt catgttgcta aaccaatgtt aagtgaatct ttggaaacaa aatgtttcca
5101 aattactggg atgtgcatgt tgaaacgtgg gttaattaac tagccatgac caaaatccct
5161 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5221 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5281 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5341 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5401 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5461 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5521 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
5581 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
5641 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
5701 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
5761 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
5821 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt cttaattaaa
5881 tttttcaaaa gtagttgaca attaatcatc ggcatagtat atcggcatag tataatacga
5941 ctcactatag gagggccatc atggccaagt tgaccagtgc tgtcccagtg ctcacagcca
6001 gggatgtggc tggagctgtt gagttctgga ctgacaggtt ggggttctcc agagattttg
6061 tggaggatga ctttgcaggt gtggtcagag atgatgtcac cctgttcatc tcagcagtcc
6121 aggaccaggt ggtgcctgac aacaccctgg cttgggtgtg ggtgagagga ctggatgagc
6181 tgtatgctga gtggagtgag gtggtctcca ccaacttcag ggatgccagt ggccctgcca
6241 tgacagagat tggagagcag ccctggggga gagagtttgc cctgagagac ccagcaggca
6301 actgtgtgca ctttgtggca gaggagcagg actgaggata agaattgtaa caaaaaaccc
6361 cgccccggcg gggttttttg ttaattaa
//
