| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001568 |
| 产品用途: | 3' AAVLINK plasmid for ARID2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARID2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARID2-FLAG 6854 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6854)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 18, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6854
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3555
/codon_start=1
/label=ARID2
/translation="GLLATR*P*QLCQIRVLHLQL*VREMQLSSLLQQELP*AERRQEL
DFQYKRFQPLKHLLLDNHHVLLLLPHSKVIK*FAKRRRKQRKQQVYMFMNVKLKSWRTR
PADEEPQTPAMGIQRKMKCMWEVF*MGESTVTQVYLLQTQGKFKVRLISAH*SVMGHHW
N*VRMEHLGNRTQNK*TCKISKVI*ENR*LMESVILIKEMVLI*AKTFQIIKLPIM*EM
VRYLQWNHKGL*ISLSKILPKVIN*KEFLMDLY*LWVVHL*AVYRRLQMRQHSNLVVLI
CLMDL*LQV*IQMCLSNAQV*LSHHILQPLLYRDIKS*QFPTQDQKYPILLPYHLTFGL
QMAQQNAKL*RGQQRILIGKQSQEFQIK*ELELLQSVTPTMLAAAQQWLLCQQEQIQAL
*LK*Q*KVLFSKSNSIHQHMYRMWSRRTLLCHLHQLYKCRASLTVLSLLHSVDPVSLEI
Q*ENLDRTSCVCGSLVKSGFRHPHRFSTMQQLNMEEKMYIQGSVFGKVVSLFSDSGFLL
LPTCRISTVQRMPYLQD*NKMNQDKQEVRSLLPSSQL*GAQAQLLEHKRPL*IIPVLHL
WL*GEDQETLSFEILQMKKRDQ*LNTSD*QLP*Y*KILVNIQNVVADC*RDMKITYQC*
PLVTWKLPPPLPNAFMNLILQFRVRNKKKTQKCC"
misc_feature 1787..1792
/label=SDS
misc_feature 1790..1795
/label=SDS
misc_feature 1963..1968
/label=SDS
misc_feature 2345..2350
/label=SDS
CDS 3562..3627
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3643..4074
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3787..3792
/label=SDS
repeat_region 4117..4257
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4117..4246
/label=AAV2 ITR
rep_origin 4332..4787
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5069..5173
/gene="bla"
/label=AmpR promoter
CDS 5174..6034
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6205..6793
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggac ttctggcaac
1561 caggtaacca taacagttgt gccaaatacg agttttgcac ctgcaactgt gagtcaggga
1621 aatgcaactc agctcattgc tccagcagga attaccatga gcggaacgca gacaggagtt
1681 ggacttccag tacaaacgct tccagccact caagcatctc ctgctggaca atcatcatgt
1741 actactgcta ctcccccatt caaaggtgat aaaataattt gccaaaagga ggaggaagca
1801 aaggaagcaa caggtttaca tgttcatgaa cgtaaaattg aagtcatgga gaacccgtcc
1861 tgccgacgag gagccacaaa caccagcaat ggggatacaa aggaaaatga aatgcatgtg
1921 ggaagtcttt taaatgggag aaagtacagt gactcaagtc tacctccttc aaactcaggg
1981 aaaattcaaa gtgagactaa tcagtgctca ctaatcagta atgggccatc attggaatta
2041 ggtgagaatg gagcatctgg gaaacagaac tcagaacaaa tagacatgca agatatcaaa
2101 agtgatttga gaaaaccgct agttaatgga atctgtgatt ttgataaagg agatggttct
2161 catttaagca aaaacattcc aaatcataaa acttccaatc atgtaggaaa tggtgagata
2221 tctccaatgg aaccacaagg gactttagat atcactcagc aagatactgc caaaggtgat
2281 caactagaaa gaatttctaa tggacctgta ttaactttgg gtggttcatc tgtgagcagt
2341 atacaggagg cttcaaatgc ggcaacacag caatttagtg gtactgattt gcttaatgga
2401 cctctagctt caagtttgaa ttcagatgtg cctcagcaac gcccaagtgt agttgtctca
2461 ccacattcta caacctctgt tatacaggga catcaaatca tagcagttcc cgactcagga
2521 tcaaaagtat cccattctcc tgccctatca tctgacgttc ggtctacaaa tggcacagca
2581 gaatgcaaaa ctgtaaagag gccagcagag gatactgata gggaaacagt cgcaggaatt
2641 ccaaataaag taggagttag aattgttaca atcagtgacc ccaacaatgc tggctgcagc
2701 gcaacaatgg ttgctgtgcc agcaggagca gatccaagca ctgtagctaa agtagcaata
2761 gaaagtgctg ttcagcaaaa gcaacagcat ccaccaacat atgtacagaa tgtggtcccg
2821 cagaacactc ctatgccacc ttcaccagct gtacaagtgc agggccagcc taacagttct
2881 cagccttctc cattcagtgg atccagtcag cctggagatc caatgagaaa acctggacag
2941 aacttcatgt gtctgtggca gtcttgtaaa aagtggtttc agacaccctc acaggttttc
3001 taccatgcag caactgaaca tggaggaaaa gatgtatatc cagggcagtg tctttgggaa
3061 ggttgtgagc cttttcagcg acagcggttt tcttttatta cccacttgca ggataagcac
3121 tgttcaaagg atgccctact tgcaggatta aaacaagatg aaccaggaca agcaggaagt
3181 cagaagtctt ctaccaagca gccaactgta gggggcacaa gctcaactcc tagagcacaa
3241 aaggccattg tgaatcatcc cagtgctgca cttatggctc tgaggagagg atcaagaaac
3301 cttgtctttc gagattttac agatgaaaaa gagggaccaa taactaaaca catccgacta
3361 acagctgcct taatattaaa aaatattggt aaatattcag aatgtggtcg cagattgtta
3421 aagagacatg aaaataactt atcagtgcta gccattagta acatggaagc ttcctccacc
3481 cttgccaaat gcctttatga acttaatttt acagttcaga gtaaggaaca agaaaaagac
3541 tcagaaatgc tgcagtgtac agactacaaa gaccatgacg gtgattataa agatcatgac
3601 atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat aatcaacctc
3661 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3721 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3781 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3841 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
3961 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
4021 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
4081 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
4141 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
4201 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
4261 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4321 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4381 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
4441 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4501 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4561 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4621 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4681 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4741 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4801 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4861 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4921 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4981 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
5041 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
5101 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
5161 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5221 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5281 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5341 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5401 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5461 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5521 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5581 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5641 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5701 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5761 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5821 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5881 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5941 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
6001 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
6061 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
6121 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6181 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6241 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6301 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6361 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6421 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6481 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6541 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6601 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6661 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6721 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6781 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6841 ttttgctcac atgt
//
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