| 质粒名称: | TVT7R(0,0) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 98631 |
| 存储实验室: | Andrew Ball, Karyn Johnson |
|
载体骨架基本信息 |
|
| 载体名称: | Andy Ball’s Vector 2,0 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | T7 |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | TVT7R(0,0) was a gift from Andrew Ball & Karyn Johnson (Addgene plasmid # 98631 ; http://n2t.net/addgene:98631 ; RRID:Addgene_98631) |
| 参考文献部分: | Characterization and construction of functional cDNA clones of Pariacoto virus, the first Alphanodavirus isolated outside Australasia. Johnson KN, Zeddam JL, Ball LA. J Virol. 2000 Jun;74(11):5123-32. PubMed 10799587 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS TVT7R(0,0) 3103 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Vector for cloning infectious RNA virus genomes.
ACCESSION .
VERSION .
KEYWORDS TVT7R(0,0)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3103)
AUTHORS Johnson KN, Zeddam JL, Ball LA
TITLE Characterization and construction of functional cDNA clones of
Pariacoto virus, the first Alphanodavirus isolated outside
Australasia.
JOURNAL J Virol. 2000 Jun;74(11):5123-32.
PUBMED 10799587
REFERENCE 2 (bases 1 to 3103)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3103
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 112..129
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(406..424)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 420..467
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(535..553)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(536..553)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(843..862)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(1072..1091)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1191..1213
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1251..1269)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1337..1441
/gene="bla"
/label=AmpR promoter
CDS 1442..2302
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1660..1679)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2473..3061
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2962..2981
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
61 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
121 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
181 attcattaat gcagggggat ctcgatcccg cgaaattaat acgactcact ataatgtctt
241 cgaattcgat atcctcgagg aagacccggg tcggcatggc atctccacct cctcgcggtc
301 cgacctgggc atccgaagga ggacgtcgtc cactcggatg gctaagggag agctcggatc
361 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
421 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
481 ctatatccgg atcgagatcc tctagagtcg acctgcaggc atgcaagctt gtattctata
541 gtgtcaccta aatcgtatgt gtatgataca taaggttatg tattaattgt agccgcgttc
601 taacgacaat atgtacaagc ctaattgtgt agcatctggc ttactgaagc agaccctatc
661 atctctctcg taaactgccg tcagagtcgg tttggttgga cgaaccttct gagtttctgg
721 taacgccgtc ccgcacccgg aaatggtcag cgaaccaatc agcagggtca tcgctagcca
781 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
841 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
901 cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
961 ctccttgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
1021 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
1081 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
1141 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
1201 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
1261 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
1321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
1381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
1441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
1501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
1561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
1621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
1681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
1741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
1801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
1861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
1921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
1981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
2041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
2101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
2161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
2221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
2281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
2341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
2401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
2461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
2521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
2581 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
2641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
2701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
2761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
2821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
2881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
2941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
3001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
3061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgc
//
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