pE194

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ATCC37128载体基本信息

出品公司: ATCC
载体名称: pE194
产品编号: 37128
生长条件: 30°C 1 day(s)
生物安全等级: 1级
宿主菌: 金黄色葡萄球菌芽孢杆菌
载体类型: 质粒
复制子: pE194 (S.aureus)
抗性: 红霉素
启动子:  
建议宿主菌:  
载体大小(kb): 3.7
多克隆位点:
备注:
克隆的限制摘要给出以下大小(kb):acci-3.8;psti-3.8。温度敏感,’对于完全诱导,质粒需要一段时间通过红霉素的亚抑制浓度诱导。金黄色葡萄球菌天然分离物,’对大环内酯、林考酰胺和B型链霉素“MLS”抗生素产生耐药性。
参考文献:
P1 
Gryczan T, et al. 1980. Characterization of chimeric plasmid cloning vehicles in Bacillus subtilis J. Bacteriol. 141: 246-253. 
P12 
Horinouchi, S., and B. Weisblum. 1980. Posttranscriptional modification of mRNA conformation : mechanism that regulates erythromycin-induced resistance. Proc. Natl. Acad. Sci. U. S. A. 77: 7079-7083. 
P454 
Iordanescu S. 1977. Relationships between cotransducible plasmids in Staphylococcus aureus. J. Bacteriol. 129: 71-75. 
P455 
Weisblum B, et al. 1979. Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J. Bacteriol. 137: 635-643. 
P456 
Horinouchi S, Weisblum B. 1982. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide and streptogramin type B antibiotics. J. Bacteriol. 150: 804-814. 
P48 
Gryczan, T. J., and D. Dubnau. 1978. Construction and properties of chimeric plasmids in Bacillus subtilis. Proc. Natl. Acad. Sci. U. S. A. 75: 1428-1432. 

ATCC37128载体图谱质粒图谱和多克隆位点信息

pE194载体图谱.png

ATCC37128载体简介

ATCC37128载体序列

LOCUS       pE194               3728 bp    DNA     circular CON 09-JUN-2022
DEFINITION  Staphylococcus aureus plasmid pE194, complete sequence.
ACCESSION   NC_005908 J01755 J01756 J01757 J01758 V01279 V01280
VERSION     NC_005908.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN14229318
KEYWORDS    RefSeq; circular; drug resistance gene; methylase; origin of
            replication; unidentified reading frame.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Horinouchi,S. and Weisblum,B.
  TITLE     Nucleotide sequence and functional map of pE194, a plasmid that
            specifies inducible resistance to macrolide, lincosamide, and
            streptogramin type B antibodies
  JOURNAL   J Bacteriol 150 (2), 804-814 (1982)
   PUBMED   6279574
COMMENT     REFSEQ INFORMATION: The reference sequence is identical to
            V01278.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 06/09/2022 03:03:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.1
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Genes (total)                     :: 5
            CDSs (total)                      :: 5
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:1280"
                     /plasmid="pE194"
     gene            join(3150..3728,1..633)
                     /locus_tag="HXH62_RS00005"
                     /old_locus_tag="pE194p1"
     CDS             join(3150..3728,1..633)
                     /locus_tag="HXH62_RS00005"
                     /old_locus_tag="pE194p1"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_011178348.1"
                     /translation="MSHSILRVARVKGSSNTNGIQRHNQRENKNYNNKDINHEETYKN
                     YDLINAQNIKYKDKIDETIDENYSGKRKIRSDAIRHVDGLVTSDKDFFDDLSGEEIER
                     FFKDSLEFLENEYGKENMLYATVHLDERVPHMHFGFVPLTEDGRLSAKEQLGNKKDFT
                     QLQDRFNEYVNEKGYELERGTSKEVTEREHKAMDQYKKDTVFHKQELQEVKDELQKAN
                     KQLQSGIEHMRSTKPFDYENERTGLFSGREETGRKILTADEFERLQETISSAERIVDD
                     YENIKSTDYYTENQELKKRRESLKEVVNTWKEGYHEKSKEVNKLKRENDSLNEQLNVS
                     EKFQASTVTLYRAARANFPGFEKGFNRLKEKFFNDSKFERVGQFMDVVQDNVQKVDRK
                     REKQRTDDLEM"
     gene            1011..1178
                     /locus_tag="HXH62_RS00010"
     CDS             1011..1178
                     /locus_tag="HXH62_RS00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cop-6 protein"
                     /protein_id="WP_006308237.1"
                     /translation="MVVDRKEEKKVAVTLRLTTEENEILNRIKEKYNISKSDATGILI
                     KKYAKEEYGAF"
     gene            1259..1859
                     /locus_tag="HXH62_RS00015"
                     /pseudo
     CDS             1259..1859
                     /locus_tag="HXH62_RS00015"
                     /inference="COORDINATES: protein motif:HMM:NF013849.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
     gene            complement(2004..2738)
                     /gene="erm(C)"
                     /locus_tag="HXH62_RS00020"
                     /old_locus_tag="pE194p5"
     CDS             complement(2004..2738)
                     /gene="erm(C)"
                     /locus_tag="HXH62_RS00020"
                     /old_locus_tag="pE194p5"
                     /EC_number="2.1.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(C)"
                     /protein_id="WP_007410443.1"
                     /translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHF
                     TLELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNI
                     PYNISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPR
                     EYFHPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKH
                     AGIDDLNNISFEQFLSLFNSYKLFNK"
     gene            complement(2798..2857)
                     /gene="ermCL"
                     /locus_tag="HXH62_RS00025"
     CDS             complement(2798..2857)
                     /gene="ermCL"
                     /locus_tag="HXH62_RS00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="WP_002497446.1"
                     /translation="MGIFSIFVISTVHYQPNKK"
ORIGIN      
        1 gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
       61 ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
      121 tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
      181 atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
      241 gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
      301 aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
      361 agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
      421 tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
      481 gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
      541 cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
      601 gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
      661 tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
      721 taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
      781 gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
      841 tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
      901 taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
      961 ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag
     1021 atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
     1081 tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
     1141 taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
     1201 ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
     1261 gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
     1321 atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
     1381 cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
     1441 aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
     1501 aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
     1561 ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
     1621 tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
     1681 ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
     1741 gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
     1801 actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
     1861 ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
     1921 gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
     1981 atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
     2041 aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
     2101 ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
     2161 taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
     2221 agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
     2281 gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
     2341 ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
     2401 ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
     2461 tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
     2521 acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
     2581 ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
     2641 ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
     2701 ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
     2761 atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
     2821 gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
     2881 gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
     2941 ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
     3001 ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
     3061 cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
     3121 tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
     3181 ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
     3241 ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
     3301 aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
     3361 gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
     3421 tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
     3481 tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
     3541 tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
     3601 aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
     3661 atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
     3721 aagcgatg
//

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