| 出品公司: | Invitrogen |
|---|---|
| 载体名称: | pYES6-CT, pYES6/CT |
| 质粒类型: | 酿酒酵母蛋白表达载体 |
| 表达水平: | 高拷贝 |
| 诱导方法: | 半乳糖 |
| 启动子: | GAL1 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5839 bp |
| 5' 测序引物及序列: | GAL1-F: AATATACCTCTATACTTTAACGTC |
| 3' 测序引物及序列: | CYC1-R: GCGTGAATGTAAGCGTGAC |
| 载体标签: | C-His, C-V5 |
| 载体抗性: | 氨苄, 杀稻瘟菌素 |
| 筛选标记: | 杀稻瘟菌素(Blasticidin) |
| 备注: | 利用半乳糖诱导蛋白在酿酒酵母中表达。 |
| 产品目录号: | V8254-20 |
| 稳定性: | 稳定 Stable |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pYES6-CT载体基本信息
pYES6-CT载体图谱质粒图谱和多克隆位点信息
pYES6-CT载体简介
pYES6/CT的是一个5.8 kb的载体，设计用来在酿酒酵母（Saccharomyces cerevisiae）中诱导表达重组蛋白。
该载体包含以下元素：
1. 酵母GAL1启动子，能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白，同时能够被葡萄糖抑制表达
2.多克隆位点可以使用的10个限制酶切位点，和两个BstX I酶切位点，便于基因插入。
3.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。
4.杀稻瘟菌素(Blasticidin)抗性基因能够方便在大肠杆菌和酵母中的进行载体筛选。
5. 载体C端编码V5抗原表位和His纯化标签，便于蛋白纯化和western blot检测。
6. 在酵母中，利用载体上的2μ复制子维持质粒的高拷贝。
pYES6-CT载体序列
LOCUS pYES6/CT 5839 bp DNA circular SYN
DEFINITION pYES6/CT
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..5839
/organism="pYES6/CT"
/mol_type="other DNA"
promoter 1..451
/label="GAL1_promoter"
misc_feature 414..437
/label="GAL1_primer"
promoter 475..493
/label="T7_promoter"
terminator 716..961
/label="CYC1_terminator"
misc_feature complement(725..743)
/label="pYESTrp_rev_primer"
misc_feature complement(725..743)
/label="CYC1_primer"
gene complement(1963..2823)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(1963..2823)
/label="ORF frame 2"
promoter complement(3341..3408)
/label="EM7_promoter"
rep_origin 3828..5299
/label="2micron_origin"
rep_origin complement(4418..5296)
/label="2micron2_origin"
ORIGIN
1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
541 AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
601 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
661 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG
721 TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG
781 TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA
841 CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA
901 TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC
961 TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
1141 GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
2821 CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT
2881 GAGTTTAGTA GACATGCAGA TCTCCCGGGA CGTGTCAGTC CTGCTCCTCG GCCACGAAGT
2941 GCTTAGCCCT CCCACACATA ACCAGAGGGC AGCAATTCAC GAATCCCAAC TGCCGTCGGC
3001 TGTCCATCAC TGTCCTTCAC TATGGCTTTG ATCCCAGGAT GCAGATCGAG AAGAACCTGT
3061 CGGCACCGTC CGCAGGGCTC AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG
3121 TCAGGTTGCC AGCTGCCGCA GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCGCAAGGTC
3181 CCCCAGTAAA ATGATATACA TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG
3241 CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT
3301 CAATGAGGGT GGATTCTTCT TGAGACAAAG GCTTGGCCAT GGTTTAGTTC CTCACCTTGT
3361 CGTATTATAC TATGCCGATA TACTATGCCG ATGATTAATT GTCAACACCG CCCTTAGATT
3421 AGATTGCTAT GCTTTCTTTC TAATGAGCAA GAAGTAAAAA AAGTTGTAAT AGAACAAGAA
3481 AAATGAAACT GAAACTTGAG AAATTGAAGA CCGTTTATTA ACTTAAATAT CAATGGGAGG
3541 TCATCGAAAG AGAAAAAAAT CAAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA
3601 TTTTTATTGC CTTTTTCGAC GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA
3661 ACCTTTAGTA CGGGTAATTA ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC
3721 TGTGCTTGGG TGTTTTGAAG TGGTACGGCG ATGCGCGGAG TCCGAGAAAA TCTGGAAGAG
3781 TAAAAAAGGA GTAGAAACAT TTTGAAGCTA TGGTGTGTGG GGCTAGCTTA TCGATGATAA
3841 GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA TACTAGATAC TCCGTCTACT
3901 GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG GCCTTACCAC TCTTTTGTTA
3961 CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA TTCTATCTTC GCGATGTAGT
4021 AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG GCACTTCTAC AATGGCTGCC
4081 ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT ATTCGAATAC GCTTTGAGGA
4141 GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC GATCGTACTT GTTACCCATC
4201 ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA ACAGATAGTA TATTTGAACC
4261 TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT GTATGTATTT CGGTTCCTGG
4321 AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC GCATCCCCGG TTCATTTTCT
4381 GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC GAAGCATCTG TGCTTCATTT
4441 TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT
4501 TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC
4561 ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA TTTTTCAAAC AAAGAATCTG
4621 AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA ACAAAGAATC
4681 TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC TAACAAAGCA
4741 TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG ATAACTTTTT
4801 GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT CTCTTCCATA
4861 AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC GGGTGCATTT
4921 TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG CATACTTTGT
4981 GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA ACGGTTTCTT
5041 CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT GTTTTCGATT
5101 CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT AGAGATAAAC
5161 ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT GGATGGGTAG
5221 GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG CAATGTTTGT
5281 GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA
5341 CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC
5401 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG CCCGAAATCG GCAAAATCCC
5461 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT CCAACAAGAG
5521 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAAGGGTCT ATCAGGGCGA
5581 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC
5641 AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
5701 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG GCTAGGGCGG TGGGAAGTGT
5761 AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT AATGGGGCGC TACAGGGCGC
5821 GTGGGGATGA TCCACTAGT
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