pYES6/CT

价格:1500元

联系方式:I47-825O-882O

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pYES6-CT载体基本信息

出品公司: Invitrogen
载体名称: pYES6-CT, pYES6/CT
质粒类型: 酿酒酵母蛋白表达载体
表达水平: 高拷贝
诱导方法: 半乳糖
启动子: GAL1
克隆方法: 多克隆位点,限制性内切酶
载体大小: 5839 bp
5' 测序引物及序列: GAL1-F: AATATACCTCTATACTTTAACGTC
3' 测序引物及序列: CYC1-R: GCGTGAATGTAAGCGTGAC
载体标签: C-His, C-V5
载体抗性: 氨苄, 杀稻瘟菌素
筛选标记: 杀稻瘟菌素(Blasticidin)
备注: 利用半乳糖诱导蛋白在酿酒酵母中表达。
产品目录号: V8254-20
稳定性: 稳定 Stable
组成型/诱导型: 诱导型
病毒/非病毒: 非病毒

pYES6-CT载体质粒图谱和多克隆位点信息

pYES6/CT载体图谱

pYES6/CT多克隆位点

pYES6-CT载体简介

pYES6/CT的是一个5.8 kb的载体,设计用来在酿酒酵母(Saccharomyces cerevisiae)中诱导表达重组蛋白。

该载体包含以下元素:

1. 酵母GAL1启动子,能够在酿酒酵母中被半乳糖高水平的诱导蛋白表达目的蛋白,同时能够被葡萄糖抑制表达

2.多克隆位点可以使用的10个限制酶切位点,和两个BstX I酶切位点,便于基因插入。

3.氨苄抗性基因能够方便在大肠杆菌中的进行载体筛选。

4.杀稻瘟菌素(Blasticidin)抗性基因能够方便在大肠杆菌和酵母中的进行载体筛选。

5. 载体C端编码V5抗原表位和His纯化标签,便于蛋白纯化和western blot检测。

6. 在酵母中,利用载体上的2μ复制子维持质粒的高拷贝。

pYES6-CT载体序列

LOCUS       pYES6/CT	5839 bp 	DNA     circular	SYN
DEFINITION  pYES6/CT
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="pYES6/CT"
                     /mol_type="other DNA"
     promoter        1..451
                     /label="GAL1_promoter"
     misc_feature    414..437
                     /label="GAL1_primer"
     promoter        475..493
                     /label="T7_promoter"
     terminator      716..961
                     /label="CYC1_terminator"
     misc_feature    complement(725..743)
                     /label="pYESTrp_rev_primer"
     misc_feature    complement(725..743)
                     /label="CYC1_primer"
     gene            complement(1963..2823)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(1963..2823)
                     /label="ORF frame 2"
     promoter        complement(3341..3408)
                     /label="EM7_promoter"
     rep_origin      3828..5299
                     /label="2micron_origin"
     rep_origin      complement(4418..5296)
                     /label="2micron2_origin"
ORIGIN
    1 ACGGATTAGA AGCCGCCGAG CGGGTGACAG CCCTCCGAAG GAAGACTCTC CTCCGTGCGT
   61 CCTCGTCTTC ACCGGTCGCG TTCCTGAAAC GCAGATGTGC CTCGCGCCGC ACTGCTCCGA
  121 ACAATAAAGA TTCTACAATA CTAGCTTTTA TGGTTATGAA GAGGAAAAAT TGGCAGTAAC
  181 CTGGCCCCAC AAACCTTCAA ATGAACGAAT CAAATTAACA ACCATAGGAT GATAATGCGA
  241 TTAGTTTTTT AGCCTTATTT CTGGGGTAAT TAATCAGCGA AGCGATGATT TTTGATCTAT
  301 TAACAGATAT ATAAATGCAA AAACTGCATA ACCACTTTAA CTAATACTTT CAACATTTTC
  361 GGTTTGTATT ACTTCTTATT CAAATGTAAT AAAAGTATCA ACAAAAAATT GTTAATATAC
  421 CTCTATACTT TAACGTCAAG GAGAAAAAAC CCCGGATCGG ACTACTAGCA GCTGTAATAC
  481 GACTCACTAT AGGGAATATT AAGCTTGGTA CCGAGCTCGG ATCCACTAGT AACGGCCGCC
  541 AGTGTGCTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC
  601 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
  661 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCCTAGA GGGCCGCATC ATGTAATTAG
  721 TTATGTCACG CTTACATTCA CGCCCTCCCC CCACATCCGC TCTAACCGAA AAGGAAGGAG
  781 TTAGACAACC TGAAGTCTAG GTCCCTATTT ATTTTTTTAT AGTTATGTTA GTATTAAGAA
  841 CGTTATTTAT ATTTCAAATT TTTCTTTTTT TTCTGTACAG ACGCGTGTAC GCATGTAACA
  901 TTATACTGAA AACCTTGCTT GAGAAGGTTT TGGGACGCTC GAAGGCTTTA ATTTGCAAGC
  961 TGCGGCCCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG
 1021 CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT
 1081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA
 1141 GAACATGTGA GCAAAAGGCC AGCAAAAGCC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC
 1201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG
 1261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT
 1321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG
 1381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG
 1441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG
 1501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC
 1561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG
 1621 GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT
 1681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG
 1741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC
 1801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT
 1861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT
 1921 TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG
 1981 TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT
 2041 CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC
 2101 GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC
 2161 CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG
 2221 GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC
 2281 AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG
 2341 ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC
 2401 TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT
 2461 GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC
 2521 AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT
 2581 ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC
 2641 TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC
 2701 TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA
 2761 AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT
 2821 CATACTCTTC CTTTTTCAAT GGGTAATAAC TGATATAATT AAATTGAAGC TCTAATTTGT
 2881 GAGTTTAGTA GACATGCAGA TCTCCCGGGA CGTGTCAGTC CTGCTCCTCG GCCACGAAGT
 2941 GCTTAGCCCT CCCACACATA ACCAGAGGGC AGCAATTCAC GAATCCCAAC TGCCGTCGGC
 3001 TGTCCATCAC TGTCCTTCAC TATGGCTTTG ATCCCAGGAT GCAGATCGAG AAGAACCTGT
 3061 CGGCACCGTC CGCAGGGCTC AAGATGCCCC TGTTCTCATT TCCGATCGCG ACGATACAAG
 3121 TCAGGTTGCC AGCTGCCGCA GCAGCAGCAG TGCCCAGCAC CACGAGTTCT GCGCAAGGTC
 3181 CCCCAGTAAA ATGATATACA TTGACACCAG TGAAGATGCG GCCGTCGCTA GAGAGAGCTG
 3241 CGCTGGCGAC GCTGTAGTCT TCAGAGATGG GGATGCTGTT GATTGTAGCC GTTGCTCTTT
 3301 CAATGAGGGT GGATTCTTCT TGAGACAAAG GCTTGGCCAT GGTTTAGTTC CTCACCTTGT
 3361 CGTATTATAC TATGCCGATA TACTATGCCG ATGATTAATT GTCAACACCG CCCTTAGATT
 3421 AGATTGCTAT GCTTTCTTTC TAATGAGCAA GAAGTAAAAA AAGTTGTAAT AGAACAAGAA
 3481 AAATGAAACT GAAACTTGAG AAATTGAAGA CCGTTTATTA ACTTAAATAT CAATGGGAGG
 3541 TCATCGAAAG AGAAAAAAAT CAAAAAAAAA AATTTTCAAG AAAAAGAAAC GTGATAAAAA
 3601 TTTTTATTGC CTTTTTCGAC GAAGAAAAAG AAACGAGGCG GTCTCTTTTT TCTTTTCCAA
 3661 ACCTTTAGTA CGGGTAATTA ACGACACCCT AGAGGAAGAA AGAGGGGAAA TTTAGTATGC
 3721 TGTGCTTGGG TGTTTTGAAG TGGTACGGCG ATGCGCGGAG TCCGAGAAAA TCTGGAAGAG
 3781 TAAAAAAGGA GTAGAAACAT TTTGAAGCTA TGGTGTGTGG GGCTAGCTTA TCGATGATAA
 3841 GCTGTCAAAG ATGAGAATTA ATTCCACGGA CTATAGACTA TACTAGATAC TCCGTCTACT
 3901 GTACGATACA CTTCCGCTCA GGTCCTTGTC CTTTAACGAG GCCTTACCAC TCTTTTGTTA
 3961 CTCTATTGAT CCAGCTCAGC AAAGGCAGTG TGATCTAAGA TTCTATCTTC GCGATGTAGT
 4021 AAAACTAGCT AGACCGAGAA AGAGACTAGA AATGCAAAAG GCACTTCTAC AATGGCTGCC
 4081 ATCATTATTA TCCGATGTGA CGCTGCAGCT TCTCAATGAT ATTCGAATAC GCTTTGAGGA
 4141 GATACAGCCT AATATCCGAC AAACTGTTTT ACAGATTTAC GATCGTACTT GTTACCCATC
 4201 ATTGAATTTT GAACATCCGA ACCTGGGAGT TTTCCCTGAA ACAGATAGTA TATTTGAACC
 4261 TGTATAATAA TATATAGTCT AGCGCTTTAC GGAAGACAAT GTATGTATTT CGGTTCCTGG
 4321 AGAAACTATT GCATCTATTG CATAGGTAAT CTTGCACGTC GCATCCCCGG TTCATTTTCT
 4381 GCGTTTCCAT CTTGCACTTC AATAGCATAT CTTTGTTAAC GAAGCATCTG TGCTTCATTT
 4441 TGTAGAACAA AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT
 4501 TTTTACAGAA CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC
 4561 ATTTTTGTAA AACAAAAATG CAACGCGACG AGAGCGCTAA TTTTTCAAAC AAAGAATCTG
 4621 AGCTGCATTT TTACAGAACA GAAATGCAAC GCGAGAGCGC TATTTTACCA ACAAAGAATC
 4681 TATACTTCTT TTTTGTTCTA CAAAAATGCA TCCCGAGAGC GCTATTTTTC TAACAAAGCA
 4741 TCTTAGATTA CTTTTTTTCT CCTTTGTGCG CTCTATAATG CAGTCTCTTG ATAACTTTTT
 4801 GCACTGTAGG TCCGTTAAGG TTAGAAGAAG GCTACTTTGG TGTCTATTTT CTCTTCCATA
 4861 AAAAAAGCCT GACTCCACTT CCCGCGTTTA CTGATTACTA GCGAAGCTGC GGGTGCATTT
 4921 TTTCAAGATA AAGGCATCCC CGATTATATT CTATACCGAT GTGGATTGCG CATACTTTGT
 4981 GAACAGAAAG TGATAGCGTT GATGATTCTT CATTGGTCAG AAAATTATGA ACGGTTTCTT
 5041 CTATTTTGTC TCTATATACT ACGTATAGGA AATGTTTACA TTTTCGTATT GTTTTCGATT
 5101 CACTCTATGA ATAGTTCTTA CTACAATTTT TTTGTCTAAA GAGTAATACT AGAGATAAAC
 5161 ATAAAAAATG TAGAGGTCGA GTTTAGATGC AAGTTCAAGG AGCGAAAGGT GGATGGGTAG
 5221 GTTATATAGG GATATAGCAC AGAGATATAT AGCAAAGAGA TACTTTTGAG CAATGTTTGT
 5281 GGAAGCGGTA TTCGCAATGG GAAGCTCCAC CCCGGTTGAT AATCAGAAAA GCCCCAAAAA
 5341 CAGGAAGATT GTATAAGCAA ATATTTAAAT TGTAAACGTT AATATTTTGT TAAAATTCGC
 5401 GTTAAATTTT TGTTAAATCA GCTCATTTTT TAACGAATAG CCCGAAATCG GCAAAATCCC
 5461 TTATAAATCA AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT CCAACAAGAG
 5521 TCCACTATTA AAGAACGTGG ACTCCAACGT CAAAGGGCGA AAAAGGGTCT ATCAGGGCGA
 5581 TGGCCCACTA CGTGAACCAT CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC
 5641 AGTAAATCGG AAGGGTAAAC GGATGCCCCC ATTTAGAGCT TGACGGGGAA AGCCGGCGAA
 5701 CGTGGCGAGA AAGGAAGGGA AGAAAGCGAA AGGAGCGGGG GCTAGGGCGG TGGGAAGTGT
 5761 AGGGGTCACG CTGGGCGTAA CCACCACACC CGCCGCGCTT AATGGGGCGC TACAGGGCGC
 5821 GTGGGGATGA TCCACTAGT
//

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pYES6-CT载体应用举例

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