| 质粒名称: | EGFP-p65 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 111190 |
| 存储实验室: | Johannes A. Schmid |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | RELA |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | EGFP（主干上的N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | EGFP-p65 was a gift from Johannes A. Schmid (Addgene plasmid # 111190 ; http://n2t.net/addgene:111190 ; RRID:Addgene_111190) |
| 参考文献部分: | Dynamics of NF kappa B and Ikappa Balpha studied with green fluorescent protein (GFP) fusion proteins. Investigation of GFP-p65 binding to DNa by fluorescence resonance energy transfer. Schmid JA, Birbach A, Hofer-Warbinek R, Pengg M, Burner U, Furtmuller PG, Binder BR, de Martin R. J Biol Chem. 2000 Jun 2;275(22):17035-42. doi: 10.1074/jbc.M000291200. 10.1074/jbc.M000291200 PubMed 10747893 |
买家导航
EGFPp65载体基本信息
EGFPp65载体图谱质粒图谱和多克隆位点信息
EGFPp65载体简介
EGFPp65载体序列
LOCUS EGFP-p65 6359 bp ds-DNA circular SYN 07-AUG-2018
DEFINITION fluorescent fusion protein.
ACCESSION .
VERSION .
KEYWORDS EGFP-p65
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6359)
AUTHORS Schmid JA, Birbach A, Hofer-Warbinek R, Pengg M, Burner U,
Furtmuller PG, Binder BR, de Martin R
TITLE Dynamics of NF kappa B and Ikappa Balpha studied with green
fluorescent protein (GFP) fusion proteins. Investigation of GFP-p65
binding to DNa by fluorescence resonance energy transfer.
JOURNAL J Biol Chem. 2000 Jun 2;275(22):17035-42. doi:
10.1074/jbc.M000291200.
PUBMED 10747893
REFERENCE 2 (bases 1 to 6359)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 7, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6359
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 119..422
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 423..626
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 577..597
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 665..674
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 671..1387
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(716..737)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(977..996)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1324..1345
/label=EGFP-C
/note="EGFP, forward primer"
CDS 2702..3073
/codon_start=1
/product="transcriptional activation domain of human RelA,
also known as p65 (O'Shea and Perkins, 2008)"
/label=RelA (p65) AD
/translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
EDFSSIADMDFSALLSQISS"
polyA_signal 3205..3326
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3242..3261)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3296..3315
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3333..3788)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3470..3491)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3682..3701
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3815..3919
/gene="bla"
/label=AmpR promoter
promoter 3921..4278
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3944..3964)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4129..4264
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4191..4210
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4313..5107
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4367..4386)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4977..4996
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5295..5314)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5339..5386
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5715..6303
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6204..6223
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta
61 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
121 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
181 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
241 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
301 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
361 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
421 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
481 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
541 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
601 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc
661 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
721 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
781 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
841 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
901 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
961 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
1021 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
1081 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
1141 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
1201 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
1261 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
1321 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
1381 gtacaagtcc ggactcagat ctcgagctca agcttccacc atggacgaac tgttccccct
1441 catcttcccg gcagagccag cccaggcctc tggcccctat gtggagatca ttgagcagcc
1501 caagcagcgg ggcatgcgct tccgctacaa gtgcgagggg cgctccgcgg gcagcatccc
1561 aggcgagagg agcacagata ccaccaagac ccaccccacc atcaagatca atggctacac
1621 aggaccaggg acagtgcgca tctccctggt caccaaggac cctcctcacc ggcctcaccc
1681 ccacgagctt gtaggaaagg actgccggga tggcttctat gaggctgagc tctgcccgga
1741 ccgctgcatc cacagtttcc agaacctggg aatccagtgt gtgaagaagc gggacctgga
1801 gcaggctatc agtcagcgca tccagaccaa caacaacccc ttccaagttc ctatagaaga
1861 gcagcgtggg gactacgacc tgaatgctgt gcggctctgc ttccaggtga cagtgcggga
1921 cccatcaggc aggcccctcc gcctgccgcc tgtcctttct catcccatct ttgacaatcg
1981 tgcccccaac actgccgagc tcaagatctg ccgagtgaac cgaaactctg gcagctgcct
2041 cggtggggat gagatcttcc tactgtgtga caaggtgcag aaagaggaca ttgaggtgta
2101 tttcacggga ccaggctggg aggcccgagg ctccttttcg caagctgatg tgcaccgaca
2161 agtggccatt gtgttccgga cccctcccta cgcagacccc agcctgcagg ctcctgtgcg
2221 tgtctccatg cagctgcggc ggccttccga ccgggagctc agtgagccca tggaattcca
2281 gtacctgcca gatacagacg atcgtcaccg gattgaggag aaacgtaaaa ggacatatga
2341 gaccttcaag agcatcatga agaagagtcc tttcagcgga cccaccgacc cccggcctcc
2401 acctcgacgc attgctgtgc cttcccgcag ctcagcttct gtccccaagc cagcacccca
2461 gccctatccc tttacgtcat ccctgagcac catcaactat gatgagtttc ccaccatggt
2521 gtttccttct gggcagatca gccaggcctc ggccttggcc ccggcccctc cccaagtcct
2581 gccccaggct ccagcccctg cccctgctcc agccatggta tcagctctgg cccaggcccc
2641 agcccctgtc ccagtcctag ccccaggccc tcctcaggct gtggccccac ctgcccccaa
2701 gcccacccag gctggggaag gaacgctgtc agaggccctg ctgcagctgc agtttgatga
2761 tgaagacctg ggggccttgc ttggcaacag cacagaccca gctgtgttca cagacctggc
2821 atccgtcgac aactccgagt ttcagcagct gctgaaccag ggcatacctg tggcccccca
2881 cacaactgag cccatgctga tggagtaccc tgaggctata actcgcctag tgacaggggc
2941 ccagaggccc cccgacccag ctcctgctcc actgggggcc ccggggctcc ccaatggcct
3001 cctttcagga gatgaagact tctcctccat tgcggacatg gacttctcag ccctgctgag
3061 tcagatcagc tccttggatc caccggatct agataactga tcataatcag ccataccaca
3121 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
3181 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
3241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
3301 ttgtccaaac tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa
3361 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
3421 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
3481 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
3541 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
3601 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
3661 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
3721 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
3781 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
3841 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
3901 atattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
3961 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
4021 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
4081 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
4141 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
4201 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
4261 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
4321 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
4381 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
4441 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
4501 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
4561 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
4621 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
4681 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
4741 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
4801 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
4861 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
4921 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
4981 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
5041 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
5101 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
5161 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
5221 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
5281 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
5341 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
5401 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
5461 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
5521 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
5581 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
5641 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
5701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
5761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
5821 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
5881 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
5941 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6001 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6061 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
6121 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
6181 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
6241 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
6301 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctca
//
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