| 质粒名称: | pAAV-hSyn-DIO-Synaptophysin-mRuby |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000849 |
| 产品用途: | express cre dependent Synaptophysin-mRuby |
| 存储实验室: | Wenjie BIAN Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | Synaptophysin-mRuby |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Adolescent sleep shapes social novelty preference in mice.
Bian, W. J., Brewer, C. L., Kauer, J. A. & de Lecea, L.
Nat. Neurosci. 2022 257 25, 912–923 (2022)
|
pAAV-hSyn-DIO-Synaptophysin-mRuby
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-hSyn-DIO-Synaptophysin-mRuby载体质粒基本信息
pAAV-hSyn-DIO-Synaptophysin-mRuby质粒图谱载体图谱和pAAV-hSyn-DIO-Synaptophysin-mRuby载体序列质粒序列多克隆位点信息
pAAV-hSyn-DIO-Synaptophysin-mRuby质粒载体简介
pAAV-hSyn-DIO-Synaptophysin-mRuby质粒序列载体序列
LOCUS pAAV-hSyn-DIO-Synaptophysin-mRuby 6550 bp DNA circular SYN 02-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6550)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6550
/mol_type="other DNA"
/organism="synthetic DNA construct"
source 845..2479
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 12..141
/label=AAV2 ITR
promoter 186..633
/label=hSyn promoter
/note="human synapsin I promoter; confers neuron-specific
expression (Kugler et al., 2003)"
primer_bind 282..304
/label=seq primer 20
protein_bind complement(709..742)
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind complement(805..838)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
CDS complement(848..1528)
/codon_start=1
/product="monomeric variant of the red fluorescent
proteineqFP611 (Kredel et al., 2009)"
/label=mRuby
/translation="MNSLIKENMRMKVVLEGSVNGHQFKCTGEGEGNPYMGTQTMRIKV
IEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVITV
MQDTSLEDGCLVYHAQVRGVNFPSNGAVMQKKTKGWEPNTEMMYPADGGLRGYTHMALK
VDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFA
GLGGG"
primer_bind 926..946
/label=seq primer 26
misc_feature complement(1556..2479)
/label=Synaptophysin
primer_bind complement(2206..2227)
/label=seq primer 27
regulatory 2476..2485
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
protein_bind 2492..2525
/label=lox2272
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GGATACTT). lox2272 sites are compatible with each
other, but incompatible with loxP or loxN sites."
protein_bind 2596..2629
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 2677..3265
/label=WPRE
/note="woodchuck hepatitis virus
posttranscriptionalregulatory element"
primer_bind complement(2730..2750)
/label=WPRE-R
/note="WPRE, reverse primer"
primer_bind complement(2730..2750)
/label=WPRE-R(通用)
CDS complement(3148..3159)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3297..3773
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3496..3515)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
primer_bind complement(3496..3515)
/label=seq primer 17
repeat_region 3813..3953
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 3813..3942
/label=AAV2 ITR
rep_origin 4028..4483
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication;
arrowindicates direction of (+) strand synthesis"
primer_bind complement(4115..4134)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4325..4346
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4343..4365)
/label=seq primer 9
primer_bind complement(4500..4519)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectablemarker"
primer_bind 4619..4641
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4679..4697)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site,
forwardprimer"
promoter 4765..4869
/gene="bla"
/label=AmpR promoter
CDS 4870..5730
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5083..5108
/label=seq primer 10
primer_bind complement(5088..5107)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
primer_bind complement(5088..5107)
/label=seq primer 12
rep_origin 5901..6489
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6390..6409
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind complement(6486..6507)
/label=seq primer 11
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtgtgtc tagactgcag agggccctgc
181 gtatgagtgc aagtgggttt taggaccagg atgaggcggg gtgggggtgc ctacctgacg
241 accgaccccg acccactgga caagcaccca acccccattc cccaaattgc gcatccccta
301 tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt gcgcactgcc agcttcagca
361 ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc accgccgcct cagcactgaa
421 ggcgcgctga cgtcactcgc cggtcccccg caaactcccc ttcccggcca ccttggtcgc
481 gtccgcgccg ccgccggccc agccggaccg caccacgcga ggcgcgagat aggggggcac
541 gggcgcgacc atctgcgctg cggcgccggc gactcagcgc tgcctcagtc tgcggtgggc
601 agcggaggag tcgtgtcgtg cctgagagcg cagtcgagaa ggtaccggat cctgtgctgt
661 ctcatcattt tggcaaagaa ttgatttgat accgcgggcc cggccgggat aacttcgtat
721 aaagtatcct atacgaagtt atatcaaaat aggaagacca atgcttcacc atcgacccga
781 attgccaagc atcaccatcg acacataact tcgtataatg tatgctatac gaagttatgt
841 cgacttaccc tccgcccagg ccggcgaact tggccacggc gtgctcccgc tgcaccacga
901 acatctcgtt gtcggactct tccagccttt ccagccggtg gtccacggcg tggatgccgg
961 gcatcttgat gttgcccacg gttttcttgg atcggtaggt ggtcacgaag ctgcagctca
1021 ggtgccctcc gccgtccacc ttcagggcca tgtgggtgta gcctctcagt ccgccgtcag
1081 cggggtacat catctcggtg ttgggctccc agcccttggt tttcttctgc atcacggcgc
1141 cgttgctggg aaaattcacg cccctgacct gggcatggta caccaggcag ccatcttcca
1201 ggctggtgtc ctgcatcacg gtgatcacgc cgccgtcctc gtatctggtc actctctccc
1261 aggtgaagcc ctcggggaag ctctgcttga agaaatcggg gatgcccttg gggtacttga
1321 tgaaggtccg gctgccgtac atgaaggatg tggccaggat gtcgaaggcg aagggcagag
1381 gtccgccctc gatcactttg atccgcatgg tctgggtgcc catgtagggg ttgccctcgc
1441 cctcgccggt gcacttgaac tggtggccgt tcacgctgcc ttccagcacc accttcatcc
1501 gcatgttttc tttgatcagg ctgttcatcg tacgggtggc gaccggtgga tccgacatct
1561 gattggagaa ggaggtgggc gcaccctgtt ggccgtagcc ttgctgccca tagtcgccct
1621 gaggcccgta gccaccgcca ccgccgctgg ctggctgccc gtaatcgggt tgataaccac
1681 cctgaggccc gtaggagtcc tggggcccat agcctccggg gccctgccca tagcccgcat
1741 cgccgtaggc atcgccagga gctggttgct tttctggggc gcctggaggt gcgcgcatga
1801 atggggcggc ccagcctgtc tccttgaaca cgaaccatag gttgccaacc cagagcacca
1861 ggttcaggaa gccaaacacc accgaggtgt tgagtcctga agtcacaggg tccctcagtt
1921 ccttgcatgt gtttcctgtc tggcggcaca taggcatctc cttgataatg ttctctgggt
1981 cagtggccat cttcacatcg gacaggcctt tggcccaggc ggatgagcta actagccaca
2041 tgaaagcgaa cactgctgtg gccaggaagt ccatcattgg ccctttgttg ttctctcggt
2101 acttgttctg caggaagatg taggtggcca gggcccccat ggagtagagg aaggcaaaca
2161 cagccacggt gacaaagaat tcagccgagg aggagtagtc accaactagg aagatcttgg
2221 tagtgccccc tttaacgcag gagggtgcat caaagtacac ttggtgcagc ctgaatgggt
2281 actcaaattc gacttcgatg ttgagggcac tctccgtctt gttggcacac tccacgctca
2341 gccgaagctc tccggtgtag ctgccgcacg tagcaaaggc gaagatggca aagacccact
2401 gcagcacctt cacgaagcca aggggctcct tgaccacccg gaactgaccc ccagccacca
2461 gctgattcac cacgtccatg gtggcgcgcc tataacttcg tataggatac tttatacgaa
2521 gttatcattg ggattcttcc tattttgatc caagcatcac catcgaccct ctagtccaga
2581 tctcaccatc gacccataac ttcgtatagc atacattata cgaagttata agcttatatg
2641 cggccgatcg atgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
2701 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
2761 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
2821 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
2881 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
2941 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
3001 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
3061 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
3121 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
3181 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
3241 atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag atctacgggt
3301 ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc actccagtgc
3361 ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag gtgtccttct
3421 ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg aagacaacct
3481 gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa tcttggctca
3541 ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc gagttgttgg
3601 gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag agacggggtt
3661 tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac ccaccttggc
3721 ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc cttctgattt
3781 tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
3841 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
3901 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
3961 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
4021 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4081 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
4141 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4201 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
4261 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4321 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg ctattctttt
4381 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
4441 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
4501 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
4561 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4621 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
4681 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
4741 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
4801 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
4861 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
4921 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
4981 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
5041 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
5101 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
5161 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
5221 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
5281 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
5341 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
5401 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
5461 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
5521 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
5581 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
5641 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
5701 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
5761 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
5821 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
5881 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
5941 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
6001 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
6061 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
6121 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
6181 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
6241 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
6301 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
6361 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
6421 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6481 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
6541 gctcacatgt
//
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