| 质粒名称: | Dendra2-Gamma-Tubulin-17 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 57721 |
| 存储实验室: | Michael Davidson |
|
载体骨架基本信息 |
|
| 载体名称: | Dendra2 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 4750 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Gamma-Tubulin,NM_001070.4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | Dendra2 |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Dendra2-Gamma-Tubulin-17 was a gift from Michael Davidson (Addgene plasmid # 57721 ; http://n2t.net/addgene:57721 ; RRID:Addgene_57721) |
| 参考文献部分: | A bright and photostable photoconvertible fluorescent protein. McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL. Nat Methods. 2009 Feb . 6(2):131-3. 10.1038/nmeth.1296 PubMed 19169260 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Dendra2-Gamma-Tubulin-17 6033 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Localization: Centrosomes, Excitation: 490 / 553, Emission: 507 /
573.
ACCESSION .
VERSION .
KEYWORDS Dendra2-Gamma-Tubulin-17
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6033)
AUTHORS McKinney SA, Murphy CS, Hazelwood KL, Davidson MW, Looger LL
TITLE A bright and photostable photoconvertible fluorescent protein.
JOURNAL Nat Methods. 2009 Feb . 6(2):131-3.
PUBMED 19169260
REFERENCE 2 (bases 1 to 6033)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6033
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 1947..2639
/codon_start=1
/product="monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya"
/label=Dendra2
/note="mammalian codon-optimized"
/translation="MNTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTA
NLTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKG
ICTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNIN
MALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARY
SPLPSQVW"
polyA_signal 2761..2882
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2798..2817)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2852..2871
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(2889..3344)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3026..3047)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 3238..3257
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3371..3475
/gene="bla"
/label=AmpR promoter
promoter 3477..3834
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(3500..3520)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 3685..3820
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3747..3766
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 3869..4663
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(3923..3942)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 4533..4552
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(4851..4870)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4895..4942
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5271..5859
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5760..5779
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcca
541 ccatgccgag ggaaatcatc accctacagt tgggccagtg cggcaatcag attgggttcg
601 agttctggaa acagctgtgc gccgagcatg gtatcagccc cgagggcatc gtggaggagt
661 tcgccaccga gggcactgac cacaaggacg tctttttcta ccaggcagac gatgagcact
721 acatcccccg ggccgtgctg ctggacttgg aaccccgggt gatccactcc atcctcaact
781 ccccctatgc caagctctac aacccagaga acatctacct gtcggaacat ggaggaggag
841 ctggcaacaa ctgggccagc ggattctccc agggagaaaa gatccatgag gacatttttg
901 acatcataga ccgggaggca gatggtagtg acagtctaga gggctttgtg ctgtgtcact
961 ccattgctgg ggggacaggc tctggactgg gttcctacct cttagaacgg ctgaatgaca
1021 ggtatcctaa gaagctggtg cagacatact cagtgtttcc caaccaggac gagatgagcg
1081 atgtggtggt ccagccttac aattcactcc tcacactcaa gaggctgacg cagaatgcag
1141 actgtgtggt ggtgctggac aacacagccc tgaaccggat tgccacagac cgcctgcaca
1201 tccagaaccc atccttctcc cagatcaacc agctggtgtc taccatcatg tcagccagca
1261 ccaccaccct gcgctaccct ggctacatga acaatgacct catcggcctc atcgcctcgc
1321 tcattcccac cccacggctc cacttcctca tgaccggcta cacccctctc actacggacc
1381 agtcagtggc cagcgtgagg aagaccacgg tcctggatgt catgaggcgg ctgctgcagc
1441 ccaagaacgt gatggtgtcc acaggccgag accgccagac caaccactgc tacatcgcca
1501 tcctcaacat catccaggga gaggtggacc ccacccaggt ccacaagagc ttgcagagga
1561 tccgggaacg caagttggcc aacttcatcc cgtggggccc cgccagcatc caggtggccc
1621 tgtcgaggaa gtctccctac ctgccctcgg cccaccgggt cagcgggctc atgatggcca
1681 accacaccag catctcctcg ctcttcgaga gaacctgtcg ccagtatgac aagctgcgta
1741 agcgggaggc cttcctggag cagttccgca aggaggacat gttcaaggac aactttgatg
1801 agatggacac atccagggag attgtgcagc agctcatcga tgagtaccat gcggccacac
1861 ggccagacta catctcctgg ggcactcagg agcagcgaat tctgcagtcg acggtaccgc
1921 gggcccggga tccaccggtc gccaccatga acaccccggg aattaacctg atcaaggagg
1981 acatgcgcgt gaaggtgcac atggagggca acgtgaacgg ccacgccttc gtgatcgagg
2041 gcgagggcaa gggcaagccc tacgagggca cccagaccgc caacctgacc gtgaaggagg
2101 gcgcccccct gcccttcagc tacgacatcc tgaccaccgc cgtgcactac ggcaaccggg
2161 tgttcaccaa gtaccccgag gacatccccg actacttcaa gcagagcttc cccgagggct
2221 acagctggga gcgcaccatg accttcgagg acaagggcat ctgcaccatc cgcagcgaca
2281 tcagcctgga gggcgactgc ttcttccaga acgtgcgctt caagggcacc aacttccccc
2341 ccaacggccc cgtgatgcag aagaagaccc tgaagtggga gcccagcacc gagaagctgc
2401 acgtgcgcga cggcctgctg gtgggcaaca tcaacatggc cctgctgctg gagggcggcg
2461 gccactacct gtgcgacttc aagaccacct acaaggccaa gaaggtggtg cagctgcccg
2521 acgcccactt cgtggaccac cgcatcgaga tcctgggcaa cgacagcgac tacaacaagg
2581 tgaagctgta cgagcacgcc gtggcccgct acagccccct gcccagccag gtgtggtaag
2641 cggccgcgac tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta
2701 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
2761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
2821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
2881 taaggcgtaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
2941 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
3001 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
3061 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
3121 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
3181 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
3241 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
3301 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg
3361 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
3421 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtcctga
3481 ggcggaaaga accagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
3541 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
3601 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
3661 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
3721 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
3781 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaagatcga
3841 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
3901 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
3961 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
4021 cgacctgtcc ggtgccctga atgaactgca agacgaggca gcgcggctat cgtggctggc
4081 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
4141 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
4201 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
4261 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
4321 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
4381 cgccaggctc aaggcgagca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
4441 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
4501 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
4561 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
4621 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
4681 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
4741 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
4801 cgcggggatc tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg
4861 aaggagacaa taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg
4921 cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc
4981 gataccccac cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc
5041 caccccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct
5101 gccatagcct caggttactc atatatactt tagattgatt taaaacttca tttttaattt
5161 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
5221 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
5281 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
5341 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
5401 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
5461 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
5521 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
5581 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
5641 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
5701 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
5761 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
5821 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
5881 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
5941 gattctgtgg ataaccgtat taccgccatg cattagttat taatagtaat caattacggg
6001 gtcattagtt catagcccat atatggagtt ccg
//
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