| 质粒名称: | p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 83485 |
| 存储实验室: | Jennifer Doudna |
|
载体骨架基本信息 |
|
| 载体名称: | p2CT |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5960 bp |
| 完整质粒大小: | 9437 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达,CRISPR |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Lbu_C2c2_R472A_H477A_R1048A_H1053A |
| 物种来源: | Leptotrichia buccalis |
| 插入大小: | 3477 bp |
| 基因突变: | R472A, H477A, R1048A, H1053A |
| GenBank编号 | GI_506250229 |
| 启动子 | -- |
| 标签/融合蛋白: | His6 MBP（插入件上的N端子） |
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | MBP forward |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A was a gift from Jennifer Doudna (Addgene plasmid # 83485 ; http://n2t.net/addgene:83485 ; RRID:Addgene_83485) |
| 参考文献部分: | Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection. East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA. Nature. 2016 Sep 26. doi: 10.1038/nature19802. 10.1038/nature19802 PubMed 27669025 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A 9437 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Bacterial expression of L. buccalis C2c2 CRISPR effector (double
HEPN nuclease 1 and 2 inactive mutant)..
ACCESSION .
VERSION .
KEYWORDS p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9437)
AUTHORS East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian
R, Doudna JA
TITLE Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA
processing and RNA detection.
JOURNAL Nature. 2016 Sep 26. doi: 10.1038/nature19802.
PUBMED 27669025
REFERENCE 2 (bases 1 to 9437)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9437
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(18..36)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 104..208
/gene="bla"
/label=AmpR promoter
CDS 209..1069
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(427..446)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 1240..1828
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 1729..1748
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 1982..1999
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 2014..2156
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(2082..2101)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 2242..2264
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(2258..2449)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind 3907..3926
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind 4010..4029
/label=T7
/note="T7 promoter, forward primer"
promoter 4010..4028
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 4117..4139
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 4159..4176
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 4186..5286
/codon_start=1
/gene="malE (mutated)"
/product="maltose binding protein from E. coli"
/label=MBP
/note="This version of the gene does not encode a signal
sequence, so MBP will remain in the cytosol."
/translation="MKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
WYAVRTAVINAASGRQTVDEALKDAQT"
primer_bind 5260..5283
/label=MBP-F
/note="Maltose binding protein, forward primer"
CDS 5341..5361
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQS"
primer_bind complement(8973..8991)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 8987..9034
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(9400..9428)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
ORIGIN
1 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
61 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
121 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
181 gcttcaataa cattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
241 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
301 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
361 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
421 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg
481 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
541 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
601 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
661 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
721 accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact
781 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
841 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
901 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
961 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
1021 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
1081 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
1141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
1201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
1261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
1321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
1381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
1441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
1501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
1561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
1621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
1681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
1741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
1801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
1861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
1921 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
1981 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
2041 tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc
2101 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
2161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
2221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
2281 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga
2341 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc
2401 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg
2461 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca
2521 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac
2581 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg
2641 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga
2701 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga
2761 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc
2821 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg
2881 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga
2941 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg
3001 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc
3061 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg
3121 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt
3181 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt
3241 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg
3301 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat
3361 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc
3421 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt
3481 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat
3541 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg
3601 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg
3661 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc
3721 cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc
3781 cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc
3841 tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc
3901 cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc
3961 ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc
4021 actataggga gaccacaacg gtttccctct agtgccggct ccggagagct ctttaattaa
4081 gcggccgccc tgcaggactc gagttctaga aataattttg tttaacttta agaaggagat
4141 atacatatga aatcttctca ccatcaccat caccatggtt cttctatgaa aatcgaagaa
4201 ggtaaactgg taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt
4261 aagaaattcg agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa
4321 gagaaattcc cacaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac
4381 gaccgctttg gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg
4441 ttccaggaca agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt
4501 gcttacccga tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac
4561 ccgccaaaaa cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag
4621 agcgcgctga tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac
4681 gggggttatg cgttcaagta tgaaaacggc aagtacgaca ttaaagacgt gggcgtggat
4741 aacgctggcg cgaaagcggg tctgaccttc ctggttgacc tgattaaaaa caaacacatg
4801 aatgcagaca ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg
4861 accatcaacg gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta
4921 acggtactgc cgaccttcaa gggtcaacca tccaaaccgt tcgttggcgt gctgagcgca
4981 ggtattaacg ccgccagtcc gaacaaagag ctggcaaaag agttcctcga aaactatctg
5041 ctgactgatg aaggtctgga agcggttaat aaagacaaac cgctgggtgc cgtagcgctg
5101 aagtcttacg aggaagagtt ggcgaaagat ccacgtattg ccgccactat ggaaaacgcc
5161 cagaaaggtg aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt
5221 actgcggtga tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg
5281 cagactaatt cgagctcgaa caacaacaac aataacaata acaacaacct cgggatcgag
5341 gaaaacctgt acttccaatc caatgcaatg aaagtgacga aggtaggagg catttcgcat
5401 aagaagtaca cgtccgaagg ccgcttagtg aagtcagaat cggaagaaaa tcgcacagac
5461 gaacgtctgt cggcgttgct taatatgcgc cttgacatgt atatcaagaa tcccagcagc
5521 acggaaacca aggaaaatca aaaacgcatt gggaaattaa agaaattctt ctcaaacaaa
5581 atggtctatc ttaaagacaa taccttgagt ttgaagaatg ggaaaaagga gaacattgat
5641 cgtgagtatt ctgagactga catccttgag agcgatgtcc gtgacaagaa aaacttcgcc
5701 gtgttgaaaa agatctatct gaatgaaaac gtgaactcgg aggaattgga agtttttcgt
5761 aacgacatta agaagaaact gaacaaaatc aacagcctga agtactcatt tgaaaagaat
5821 aaggcgaatt atcaaaagat taatgagaat aacatcgaga aggttgaagg taagtcaaag
5881 cgtaacatta tttacgatta ttatcgtgag tcagcgaaac gtgacgctta tgtaagcaat
5941 gtgaaagaag cctttgataa gctttacaag gaagaggaca ttgcaaaact tgttcttgaa
6001 attgagaacc ttacgaagtt agagaaatac aagattcgcg agttctacca cgaaattatt
6061 ggacgtaaga atgacaagga aaactttgca aaaatcatct acgaagaaat ccagaatgtt
6121 aataacatga aagagttgat cgagaaggta ccggacatga gtgagttgaa aaagagccaa
6181 gtattttaca agtattactt agacaaagaa gagttgaacg acaagaacat caaatacgcg
6241 ttttgtcatt tcgtggaaat cgaaatgagt cagttgctga agaactacgt atataagcgc
6301 ttaagtaata tctcgaatga caaaattaag cgtatctttg aataccagaa cttgaaaaaa
6361 ttgatcgaaa ataagctgtt aaacaaactt gacacgtacg tccgtaattg tggaaagtat
6421 aattattatt tgcaagacgg cgaaattgcc acttcagatt tcatcgcccg caaccgtcag
6481 aatgaagcgt ttcttcgcaa catcattggg gtgtcatctg tggcctactt ttctcttcgc
6541 aacattcttg aaacggagaa cgagaatgat attactgggc gtatgcgcgg caaaacagtt
6601 aagaacaata aaggtgaaga gaagtacgtg tccggagaag ttgataagat ctataatgaa
6661 aataagaaga acgaggttaa ggagaactta aaaatgttct attcgtacga tttcaatatg
6721 gacaacaaga atgaaatcga agatttcttc gccaacatcg acgaggcgat ttcttccatc
6781 gctcacggta ttgtcgcctt caacttggaa ttagaaggta aggatatctt tgcgttcaag
6841 aacattgcgc catccgaaat ctcaaagaag atgtttcaga atgagattaa cgagaaaaaa
6901 ctgaaattga agatctttcg tcaactgaac tctgccaacg tgttccgcta tctcgaaaag
6961 tataaaattc tgaattacct taaacgtaca cgcttcgagt ttgtcaataa aaatatccca
7021 ttcgtcccgt ctttcaccaa attatattcg cgcattgatg acctgaagaa tagtcttggg
7081 atttactgga aaactccgaa aacaaacgac gacaataaga ctaaggagat tattgatgcc
7141 caaatctatt tgcttaaaaa catctattac ggggagttcc tgaattattt catgtcgaac
7201 aatggtaatt tctttgagat ttctaaagaa atcatcgaat tgaacaagaa cgataaacgc
7261 aacttaaaga ctgggtttta caagctgcaa aagtttgaag acatccagga gaagattcca
7321 aaggaatact tggcgaatat ccagtccctg tacatgatta atgccggtaa tcaggacgaa
7381 gaagaaaagg acacttatat tgatttcatt caaaagatct tcttaaaggg atttatgacg
7441 tatcttgcta ataacggtcg tttaagtctg atttacatcg gctcggatga agaaacaaat
7501 acgtcattag cagaaaagaa gcaagagttt gacaagttct tgaagaagta cgagcagaac
7561 aataatatca agatccccta tgagatcaat gaattcctgc gtgagatcaa actgggaaac
7621 atcctgaagt atactgagcg tttaaacatg ttctacctta tcttaaagct tttgaatcac
7681 aaggagctga caaatctgaa gggtagtctt gaaaaatatc agtctgccaa taaggaagaa
7741 gcgttctctg accaattgga gttaattaac ctgcttaacc ttgacaacaa ccgcgtgacg
7801 gaagacttcg aattagaggc cgacgagatt ggaaaatttc ttgatttcaa tggcaacaaa
7861 gttaaggata acaaggaact gaaaaagttc gatacaaaca agatctactt tgacggcgag
7921 aacattatca aacaccgtgc cttctacaat attaagaaat atggcatgtt aaacttactg
7981 gagaaaattg ccgacaaggc tggatacaag atctcgatcg aagagctgaa gaaatactcc
8041 aataaaaaga atgagatcga gaagaaccat aagatgcagg aaaatctgca ccgcaaatac
8101 gctcgtcccc gtaaagacga gaagtttaca gatgaggact atgaaagtta caagcaagct
8161 attgagaata ttgaggagta cacccacctt aagaacaagg tagaattcaa tgagctgaat
8221 ttactgcagg gcctgttgct gcgcatttta catcgtttag tcggatatac ctcaatttgg
8281 gaacgcgatc tgcgcttccg ccttaaaggt gagttcccag aaaaccaata catcgaagag
8341 atcttcaact ttgaaaataa gaagaacgtg aagtacaaag ggggtcagat tgtagagaaa
8401 tacattaaat tctacaagga attacatcaa aatgatgaag ttaagatcaa caagtacagt
8461 tccgcgaata tcaaggtgtt gaagcaagaa aagaaggacc tttatattgc taattacatc
8521 gccgcattca attatattcc tcacgccgag atctcactgc tggaagtcct tgaaaatttg
8581 cgtaaattgc tgtcctacga tcgcaaactg aaaaatgccg taatgaaatc agtagttgat
8641 atccttaagg agtatggttt tgtagccaca ttcaaaatcg gggcggacaa gaagatcggt
8701 attcagacac tggagagcga aaaaatcgtg catcttaaga atcttaagaa gaagaagtta
8761 atgactgacc gcaattccga ggaactttgc aaattggtga agattatgtt tgaatacaaa
8821 atggaagaga aaaagtctga aaactaataa cattggaagt ggataacgga tccgcgatcg
8881 cggcgcgcca cctggtggcc ggccggtacc acgcgtgcgc gctgatccgg ctgctaacaa
8941 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
9001 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata
9061 tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag
9121 cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata
9181 gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg
9241 aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
9301 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
9361 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa
9421 gctgtcaaac atgagaa
//
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