| 质粒名称: | pMo130-TelR |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 50799 |
| 存储实验室: | Kim Lee Chua |
|
载体骨架基本信息 |
|
| 载体名称: | pMo130 |
| 载体出品公司: | Martin Voskuil |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 6127 bp |
| 完整质粒大小: | 9392 bp |
| 载体修饰: | -- |
| 载体类型: | 自杀载体 |
| 筛选标记: | 亚碲酸盐 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | tellurite-resistance cassette,TelR |
| 物种来源: | RK2 plasmid |
| 插入大小: | 3266 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | PCs12 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XmaI(未破坏) |
| 3' 克隆位点: | XmaI(未破坏) |
| 5' 测序引物 | 5'-CGGGTACCGAGCTCGAATTA-3' |
| 3' 测序引物 | 5'-CCGGGTACCGAGCTCGAATT-3' |
|
文献引用方法 |
|
| 材料和方法部分: | pMo130-TelR was a gift from Kim Lee Chua (Addgene plasmid # 50799 ; http://n2t.net/addgene:50799 ; RRID:Addgene_50799) |
| 参考文献部分: | A method for generating marker-less gene deletions in multidrug-resistant Acinetobacter baumannii. Amin IM, Richmond GE, Sen P, Koh TH, Piddock LJ, Chua KL. BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158. 10.1186/1471-2180-13-158 PubMed 23848834 |
买家导航
pMo130TelR载体基本信息
pMo130TelR载体图谱质粒图谱和多克隆位点信息
pMo130TelR载体简介
pMo130TelR载体序列
LOCUS pMo130-TelR 9409 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Suicide vector for generating chromosomal deletions in
multidrug-resistant bacteria.
ACCESSION .
VERSION .
KEYWORDS pMo130-TelR
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9409)
AUTHORS Amin IM, Richmond GE, Sen P, Koh TH, Piddock LJ, Chua KL
TITLE A method for generating marker-less gene deletions in
multidrug-resistant Acinetobacter baumannii.
JOURNAL BMC Microbiol. 2013 Jul 13;13:158. doi: 10.1186/1471-2180-13-158.
PUBMED 23848834
REFERENCE 2 (bases 1 to 9409)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9409
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 972..1003
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
protein_bind complement(1067..1114)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
rep_origin complement(1293..1881)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(1373..1392)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
oriT 2181..2290
/note="incP origin of transfer"
terminator 3541..3586
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
protein_bind 5078..5094
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS complement(5153..5947)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5264..5283)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5874..5893
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(5948..6051)
/gene="bla"
/label=AmpR promoter
primer_bind 6119..6137
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(6175..6197)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
promoter complement(7240..7268)
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind complement(7319..7366)
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
RBS 7458..7480
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
1 atgaatgcca caaacaccga tgttttcgcc caggtaggcg gcctggaggc ccgaggcgcg
61 aagatgaaga agcggggcac ccgcttcctc atcgcggcgc tggcagtcct tgccattgcc
121 gggatcgggg cagtaacggg atgggcgatc agcccgagcg cgacgcccgg aagcattgac
181 gtgccgcagg tgctggcatc gacattcagc gaccaggtgc cgggcagtga gggcggcggc
241 ctgggtggcg gcctgccctt cacttcggcc gtcggggcat tcacggactt catggcgggg
301 ccggcaattt ttaccttggg cattcttggc atagtggtcg cgggtgccgt gctcgtgttc
361 gggggtgagt tctgcgggtt cgtgcgatcc gtctgcatga tggtgatagc cgtcagcatg
421 attttcgtgt cgtcgaactt ggtgaagggc attctcggcg gcgatcacga cgccggccct
481 gcggagcctt cgccgcgtgc gcgattcatg gcggccgtgg aggccaagga tttcgcgcga
541 gtgcaagagc tgatcgaggc gcgtggagcc aagtcggcgg ctgattatgt ccttgcgcag
601 ctcgccgtgg ccgaaggtct ggaccgcaag cctggtgcgc gcgtcgtggt cgggaaagcg
661 gcgggcagca tggcaatgcc gcctgcggcg ctgggtttta cgccaagggg agaagcggca
721 tacgccatcg agcggtcagc ctatggtgag ccgaggtcca gcattgcgaa gcagtaccag
781 caggaatgga accggaaggc ggcgacctgg tgggcgatgg ccggtgtggc cggcatcatc
841 ggcgcgatcc tggcggcggc ggcaaccggc tttgttgggc tggcagtgtc gatccgcaac
901 cgagtgaagc gcgtgcgcga cctgttggtg atggagccgg gtgcagagcc ataagggatc
961 gatccccctc aagtcaaaag cctccggtcg gaggcttttg actttctgct cgaggagcta
1021 attccattga gtaagttttt aagcacatca gcttcaaaag cgctctgaag ttcctatact
1081 ttctagagaa taggaacttc ggaataggta cttcaagatc cccaattcga gctcggtacc
1141 cgggaagatc tggctagcgg ggccctgata tcagctcact caaaggcggt aatacggtta
1201 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
1261 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
1321 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
1381 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
1441 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
1501 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
1561 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
1621 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
1681 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
1741 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
1801 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
1861 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
1921 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
1981 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
2041 tggtctgaca gttattaatt aatcctcaat cgctcttcgt tcgtctggaa ggcagtacac
2101 cttgataggt gggctgccct tcctggttgg cttggtttca tcagccatcc gcttgccctc
2161 atctgttacg ccggcggtag ccggccagcc tcgcagagca ggattcccgt tgagcaccgc
2221 caggtgcgaa taagggacag tgaagaagga acacccgctc gcgggtgggc ctacttcacc
2281 tatcctgccc ggctgacgcc gttggataca ccaaggaaag tctacacgaa ccctttggca
2341 aaatcctgta tatcgtgcga aaaaggatgg atataccgaa aaaatcgcta taatgacccc
2401 gaagcagggt tatgcagcgg aaaagcgctg cttccctgct gttttgtgga atatctaccg
2461 actggaaaca ggcaaatgca ggaaattact gaactgaggg gacaggcgag agacgatgcc
2521 aaagagctac accgacgagc tggccgagtg ggttgaatcc cgcgcggcca agaagcgccg
2581 gcgtgatgag gctgcggttg cgttcctccg cggcagatgt ctgcctgttc atccgcgtcc
2641 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
2701 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
2761 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
2821 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
2881 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
2941 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
3001 ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag
3061 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
3121 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
3181 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
3241 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
3301 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
3361 gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg
3421 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
3481 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgga attctcagat
3541 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgaaag ctactattat
3601 ttgttaactg ttaattgtcc ttgttcaagg atgctgtctt tgacaacaga tgttttcttg
3661 cctttgatgt tcagcaggaa gctcggcgca aacgttgatt gtttgtctgc gtagaatcct
3721 ctgtttgtca tatagcttgt aatcacgaca ttgtttcctt tcgcttgagg tacagcgaag
3781 tgtgagtaag taaaggttac atcgttagga tcaagatcca tttttaacac aaggccagtt
3841 ttgttcagcg gcttgtatgg gccagttaaa gaattagaaa cataaccaag catgtaaata
3901 tcgttagacg taatgccgtc aatcgtcatt tttgatccgc gggagtcagt gaacaggtac
3961 catttgccgt tcattttaaa gacgttcgcg cgttcaattt catctgttac tgtgttagat
4021 gcaatcagcg gtttcatcac ttttttcagt gtgtaatcat cgtttagctc aatcataccg
4081 agagcgccgt ttgctaactc agccgggcgt tttttatcgc tttgcagaag tttttgactt
4141 tcttgacgga agaatgatgt gcttttgcca tagtatgctt tgttaaataa agattcttcg
4201 ccttggtagc catcttcagt tccagtgttt gcttcaaata ctaagtattt gtggccttta
4261 tcttctacgt agtgaggatc tctcagcgta tggttgtcgc ctgagctgta gttgccttca
4321 tcgatgaact gctgtacatt ttgatacgtt tttccgtcac cgtcaaagat tgatttataa
4381 tcctctacac cgttgatgtt caaagagctg tctgatgctg atacgttaac ttgtgcagtt
4441 gtcagtgttt gtttgccgta atgtttaccg gagaaatcag tgtagaataa acggattttt
4501 ccgtcagatg taaatgtggc tgaacctgac cattcttgtg tttggtcttt taggatagaa
4561 tcatttgcat cgaatttgtc gctgtcttta aagacgcggc cagcgttttt ccagctgtca
4621 atagaagttt cgccgacttt ttgatagaac atgtaaatcg atgtgtcatc cgcattttta
4681 ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat agtttgcgac agtgccgtca
4741 gcgttttgta atggccagct gtcccaaacg tccaggcctt ttgcagaaga gatattttta
4801 attgtggacg aatcaaattc agaaacttga tatttttcat ttttttgctg ttcagggatt
4861 tgcagcatat catggcgtgt aatatgggaa atgccgtatg tttccttata tggcttttgg
4921 ttcgtttctt ccgcaaacgc ttgagttgcg ccgtcgctct gcgcgtgcgc cgacatgcaa
4981 gcggctgcgg tgagcgcggc ggcgctcagg gccaaacgtt tgtgtctacg ggaaacggct
5041 tgtttggagt acgtcatcgt ttcctcctaa cgcttaattg ttatccgctc acaattccac
5101 acattaaact agattcgatg attaattgtc aacagctcac tagtttaatt aatcagaaga
5161 actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa
5221 gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca
5281 acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa
5341 agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat
5401 cctcgccgtc gggcatccgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct
5461 gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc
5521 gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca
5581 gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca
5641 ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa
5701 cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct
5761 cgtcttggag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc
5821 cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt
5881 catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt
5941 caatcatact cttccttttt caaattattg aagcatttat cagggttatt gtctcatgag
6001 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
6061 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
6121 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
6181 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
6241 agcccgtcgc atgcatctag agggatccgc tgcagggcgg ccgctcaggt cagcacggtc
6301 atgaatcgtt cgttgagaat gcggtcgtgg taaaagatcg ccttgcccag ctggtcggtg
6361 gtccaggtca ccggtttgtg gtccgggtag ttgtaatctc ccccgcagaa cacttcgttg
6421 cggttaccgg acgggtcgaa gaagtagatg gtcttgccgt gagtgaggcc gtggcgggtt
6481 gggccgatat cgatagatgt gtcggtcatg gagatcaggt cggcggcgcg aagcaagtct
6541 tcccaggttt cgaggtggaa ggacacatga tggaggcggc ctttttccgg atggtgaatg
6601 aaggccacgt cgtgggcctt ggtcgacaga ctgagaaact gggcgacgcg cgtgccattt
6661 tcgtccagca cctgttcggc cagatagaaa ccgagcacct tggtgaacag gtcataggtc
6721 gccggcaatt cgtcgccata catgagggcg tggtcgaaac gcacagccgc catacctttc
6781 agatcgcgcg gccatgcctc gggattgacg tcattcaaac cccactttcc agtatattcc
6841 ttgtctgcat acaactcgaa gtgatgcccg gagggggcct ggaagcgcac gcgccggcca
6901 caactgttca gttcacctgc gggtagctgc tcaacggcac agccatatgc catcagatcc
6961 cgctccagtt gccggagagc atcctcatcc acaaccttga aacccataaa atccatgccc
7021 ggctcgtcag cctcgcgtag caccagggaa aacttatcca cttcggtcca agccttcaga
7081 tagacacggc cctggtcgtc acggtccatc tcgatcaggc ccagcaactc gacgtagtgt
7141 tccagggcct tgctcatgtc cagtacacgc agctgcacat ggcccggtcg cattacacct
7201 ttgttcatag tattctgtct ccttctgtga tattccacac attatacgag ccgatgatta
7261 atggtcaaca gctcaagctt cccgggtacc gagctcgaat tagcttcaaa agcgctctga
7321 agttcctata ctttctagag aataggaact tcggaatagg aacttcaaga tcccctgatt
7381 ccctttgtca acagcaatgg atatcagccg ttgacttagt tggtatttcc ggaatatcat
7441 gctgggttcc gaataatttt gtttaacttt aagaaggaga tataccatgg aagaacaagc
7501 gtgaacatgg cgcgattgaa gggggaggtt ttgcccgccc tcttcgcgtc gccggcgacg
7561 attggcgagt acggggccgg catcgacggg gcggattccc tcaacgagct gtcgaatctg
7621 atggagcacg gcgcagttgc cgcgctggcc gacaaaatca gccagatcgt ggcgaagctg
7681 gtcgacgcgg acccccgcaa gatcgcggaa aagcctacct ggttcgagaa gatgcttggc
7741 cgtgaggttg aacgccaggt gaggtatcag gtcgcccgca agacgctcga ccagttgctg
7801 gacgaagccg agggcgtagc gcagcgcgtg cgggacacgt tgcgcgcctt ggatgacatg
7861 ctcaatacgc atgaggccga ggtagaccgg ctcagagcct acattcaagc cgggcgcgag
7921 ttcctggacg agaaccccga ggccggcgcg gccaaggccg gcgtgatcga gttcgacaag
7981 ccgcgcgaac gcttcgcgcg caagctcgcc aacctggcaa ccctcatggc gtcccatgaa
8041 atgagcgtca ctcagatgaa gctcacgcgg gcgcaggccg tggacatgct ggaccgcttc
8101 tctgaaacgg catccgtcct ggtgcccgtc tggcgtcagc acaccctcgc gctcatcacc
8161 accaagaaca tgaatccggc aatggtcgcc gaggcggcca aagctcacca ggcgctcatg
8221 cggagccttt cgcagagcct ggaaggcatc aaccaataac acggcgggag aaccctatga
8281 acgcactgaa aacgacgcac gacgccaagg cccctatcgt cgcctttgac atgaccccgg
8341 caaccctgcg cgagctgggc ttgcaggaaa gcgacgtgcc ggaagtccat gcggtcgcgc
8401 agcggatcga ggtcggcagt ccgcagaccg ttgccgagtt cggccgcgac gtggccgagc
8461 acacgtcccg ctacgccgat agcctgctgg accaggtgcg caacagcgac ctggacgaag
8521 caggcgagaa actgacccag gttgtcgcca aggcccgttc cctgaacgtc ggccctttgt
8581 ccgacaaccg ttcccgcctg cccctgattg gcccgctgat cgaccgctcc cgcgtccgtt
8641 cgacgggctt catggcgcgc ttcgacacga cccgcgagca gatcgaacac ctggtcagcg
8701 aagtgcagac cacccagcaa ggcatcgcgc agcgcaatgc ctcgctcgac gaaatgttcg
8761 cagccgtgcg cgaggaacac cgccttcttg gcgtccacat cgcggccggc aaggtccgcc
8821 ttgccgagct gcgcgagcag gccgagggtc tgcgcggcaa tgtcgggaac gaccctggcc
8881 gcgtgcagga gctggccgac ctcgatgcga tggttgccaa cctggacaag cgcatcggcg
8941 acctgatcgc cttgcaacat tcggccatgc agagcctgcc gaccatccgc atgatccagg
9001 ccaacaacca gatgctggtc gataaattcc acaccatccg cgaaatcacc gtgccggcgt
9061 ggaagcggca attcatgctg gccttgagcc tcaacgagca gaagaacgcc gtcgaactgg
9121 ccacggccat cgacgacacc accaacgacc tgatgaagcg caatgcggcc ctgctgcatc
9181 gcacgtccgt cgagacggcg aaggagaacc aacgcctggt gatcgacgtg gacacgctca
9241 agcaggttca gacgacgctc atcaagaccg tcgaggacgt tattcgcatc cagcaggaag
9301 gcgtgcagaa gcgcaaggat gccgagaagc agatcgccgc aatgcgtggc gatcttcaag
9361 ccaagctgac ccgccagccc gtgcgcgagc tggcccaaca ggagtccgt
//
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